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Lee, Jienny,Byeon, Jeong Su,Lee, Keum Sil,Gu, Na-Yeon,Lee, Gyeong Been,Kim, Hee-Ryang,Cho, In-Soo,Cha, Sang-Ho Springer-Verlag 2016 Veterinary research communications Vol.40 No.1
<P>Mesenchymal stem cells (MSCs) have the ability to differentiate into multi-lineage cells, which confers great promise for use in regenerative medicine. In this study, canine adipose MSCs (cAD-MSCs) were isolated from canine adipose tissue. These cells clearly represented stemness (Oct4, Sox2, and Nanog) and differentiation potential into the mesoderm (adipocytes, chondrocytes, and osteoblasts) at early passages. The aim of this study was to evaluate the effects of hypoxia on the differentiation potential into mesoderm, and the expression of anti-apoptotic genes associated with cell survival for the optimal culturing of MSCs. We observed that the proliferation of the cAD-MSCs meaningfully increased when cultured under hypoxic condition than in normoxic condition, during 7 consecutive passages. Also, we found that hypoxia strongly expressed anti-senescence related genes such as HDAC1 (histone deacetylase 1), DNMT1 (DNA (cytosine-5)-methyltransferase 1), Bcl-2 (inhibitor of apoptosis), TERT (telomerase reverse transcriptase), LDHA (lactate dehydrogenase A), SLC2A1 (glucose transporter), and DKC1 (telomere holoenzyme complex) and differentiation potential of cAD-MSCs into chondrocytes, than seen under the normoxic culture conditions. We also examined the multipotency of hypoxic conditioned MSCs using quantitative real-time RT-PCR. We found that the expression levels of stemness genes such as Oct-4, Nanog, and Sox-2 were increased in hypoxic condition when compared to the normoxic condition. Collectively, these results suggest that hypoxic conditions have the ability to induce proliferation of MSCs and augment their chondrogenic potential. This study suggests that cell proliferation of cAD-MSC under hypoxia could be beneficial, when considering these cells for cell therapies of canine bone diseases.</P>
신생아 중환자실 개방면회와 개방면회에 대한 부모 불안, 간호사의 태도
이유나(Yu-nah Lee),김은숙(Eunsook Kim),박소현(Sohyun Park),김안나(An Na Kim),이지윤(Jiyoon Lee),이금문(Keum Moon Lee),최수정(Sujung Choi),이은정(Eun-jung Lee) 한국근거기반간호학회 2014 근거와 간호 Vol.2 No.1
Purpose: The purpose of this study was to survey the visitation conditions in the Neonatal Intensive Care Unit (NICU) and to evaluate parents’ anxiety and nurses’ attitude about the open visitation policy. Methods: One group pre-posttest design was used. After the approval of the institutional review board was obtained, 22 parents and 58 nurses in the NICU participated in the survey between January and February in 2014.The visitation conditions were the number of patients who had visitors in the NICU for a whole day, total visitation hours, mean visitation hours and total number of visitation in a day. Before and after the open visitations were applied, the anxiety level of the patients and attitudes of the nurses were measured. Results: After the open visitation, all visitation conditions significantly increased. Parents’ anxiety score also significantly decreased after experiencing the open visitation (p=.023). However, nurses’ attitude was not different (p=.851). Conclusion: The open visitation can relive parents’ anxiety in the NICU but still nurses have some conflicts for applying the open visitation. It is necessary to prepare environmental infrastructure and educate nurses and parents to activate the open visitation in the NICU.
( Chan Hyuk Park ),( Jung Hwa Lee ),( Na Keum Lee ),( Yong Chan Lee ),( Sang Kil Lee ) 대한간학회 2016 Gut and Liver Vol.10 No.5
Background/Aims: Although LIN28A is known to potentially play a role in the oncogenesis of various cancers, whether LIN28A expression is a predictor of poor prognosis in patients with gastric cancer has not been fully explored. We sought to evaluate clinicopathological characteristics according to the expression of LIN28A in numerous gastric cancer tissue samples. Methods: LIN28A expression was evaluated by immunohistochemical (IHC) analysis of a tissue microarray comprising 288 gastric cancer tissues and 288 adjacent normal tissues. Clinicopathological characteristics, including overall survival, were compared according to LIN28A expression. Results: The IHC staining score was lower for the cancer tissues than the normal tissues (p<0.001). However, no significant differences were observed in the clinicopathological characteristics between the low and high LIN28A expression groups. In addition, the 5-year overall survival rate did not differ between the two groups: 75.3% (95% confidence interval [CI], 69.3% to 81.7%) versus 71.6% (95% CI, 63.3% to 80.9%) for low versus high expression, respectively. Conclusions: The expression of LIN28A did not appear to play a distinct role in predicting the clinicopathological characteristics of patients with gastric cancer. In addition, LIN28A expression was not an independently associated factor for overall survival in patients with gastric cancer. (Gut Liver 2016;10:714-718)
( Chan Hyuk Park ),( Jung Hwa Lee ),( Na Keum Lee ),( Yong Chan Lee ),( Sang Kil Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Gastric cancer is one of the major causes of cancer death worldwide; however, the mechanism of carcinogenesis is complex and poorly understood. Recently long non-coding was emerged as promoters of metastasis in various cancers including gastric cancer. Here we investigated the impact of lung adenocarcinoma transcript-1 (MALAT-1) on apoptosis, cell proliferation and cell cycle to dissect the carcinogenesis of gastric cancer and examined mechanism of invasion and metastasis. Methods: Expression of MALAT-1 and U6 was determined by SYBR qRT-PCR in twenty two gastric cancer cell lines and fi fty fresh cancer tissues and adjacent tissues. Down regulation of MALAT-1 was confi rmed by two different siRNAs. Cell counts and proliferation was determined after treatment of siRNAs. FACS using PI/Annexin-V staining carried out. Scratch wound healing assay and the matrigel invasion assay was done. Results: The expression of MALAT-1 was signifi cantly elevated in various gastric cancer cell lines and gastric cancer tissues compared to normal (P<0.01). SiR-MALAT-1 significantly reduced viable AGS cell and induces apoptosis (p<0.05). Deep invasion of tumor (advanced T stages) was more common in the high level of MALAT-1 group (p<0.05). siR-MALAT-1 signifi cantly decreased the AGS cell invasiveness and migration. siR-MALAT-1 reduced expression of snail and N-cadherin and elevated E-cadherin. Conclusions: MALAT-1 was involved in gastric carcinogenesis via inhibition of apoptosis and promoted invasiveness via epithelial-to-mesenchymal transition.
Bioactivities of the Herb Extracts Used for Gamhongroju, a Korean Liqueur
Lee, Sae-Rom,Jung, Ha-Na,Cho, Hyunn-Ho,Jhin, Chang-Ho,Hwang, Keum-Taek,Jeong, Seok-Tae,Kim, Tae-Young The Korean Society of Food Science and Nutrition 2011 Preventive Nutrition and Food Science Vol.16 No.4
In this study, antioxidative and anti-inflammatory activities of the herb (cinnamon, clove, glehnia root, ginger, violet-root cromwell, licorice, citrus peel and longan) extracts used for gamhongroju, one of the popular liqueurs in Korea, were investigated. Twenty grams of individual herbs were extracted in 60% purified ethanol and freeze-dried. A mixture of the individual herb extracts (HEM) was separately prepared. Cytotoxicity of the individual extracts and HEM on murine RAW264.7 macrophage cells were examined along with their recovering activity on $H_2O_2$-treated RAW264.7 cells. Antioxidant and anti-inflammatory activities of the extract-treated cells were determined by measuring superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, and Trolox equivalent antioxidant capacity (TEAC), nitric oxide (NO) and prostaglandin E2 (PGE2) levels. Violet-root cromwell extract showed the least cytotoxicity in terms of treated concentration. Most of the extracts, below levels of cytotoxicity, recovered the $H_2O_2$-treated cells. Treatment with some of the extracts increased SOD and GPx activities and TEAC levels while a majority inhibited the production of NO and PGE2 in lipopolysaccharide (LPS)-treated cells.
Lee Hye Mi,Hyeon Hee Lee,Young Cheon Na,Choi Keum Ha 대한창상학회 2024 Journal of Wound Management and Research Vol.20 No.1
Background: Keloid and hypertrophic scars are prominent scars that are excessively repaired with upregulated synthesis, deposition, and accumulation of collagen. Topical agents are used to reduce inflammation and fibrotic changes via reduced transepithelial water loss. Increased mechanical pressure applied through scar massage can accelerate scar maturation by inducing fibroblast proliferation, which enhances the remodeling of connective tissue matrices and collagen degradation.Methods: This study comparatively analyzed the effectiveness of topical agents applied to post-burn wounds on the dorsal surface of Sprague-Dawley rats through twice-daily massaging. Postoperative histological analysis of the tissues was performed after surgical en bloc removal of the treatment area at 4, 10, and 16 weeks.Results: Histological analyses revealed larger amounts of fibroblasts in Mepiform and Scarnos gel-treated tissue than in Vaseline-treated tissue. Granulation was prevented in scars treated with the topical agents.Conclusion: Mepiform Ultra scar gel and Scarnos gel, accompanied by massaging, may be effective anti-scarring topical agents to alleviate contact burn scars in Sprague-Dawley rats.
헤스페리딘을 함유한 락타이드 글리콜라이드 공중합체 지지체에서 섬유륜 세포의 증식과 거동 평가
장나금(Na Keum Jang),고현아(Hyun Ah Ko),차세롬(Se Rom Cha),이선의(Seon Eui Lee),정현기(Hyun Ki Jeong),김수영(Su Young Kim),신재훈(Jae Hun Shin),김은영(Eun Young Kim),송정은(Jeong Eun Song),강길선(Gilson Khang) 한국고분자학회 2015 폴리머 Vol.39 No.5
항산화와 항염증으로 잘 알려진 헤스페리딘은 디스크 재생을 위해 효과적인 지지체로 제작하기 위해 사용하였다. 지지체는 poly(lactide-co-glycolic acid) (PLGA)에 헤스페리딘(0, 3, 5, 10%)을 첨가하여 염 침출법으로 제작하였고, 섬유륜 세포(AF)를 파종한 후 세포의 변화를 연구하였다. SEM, WST 그리고 RT-PCR 분석을 통해 세포의 부착과 증식, 세포의 표현형 분석을 실시하였다. 또한 면역조직화학염색을 통하여 AF세포의 생체 내 거동을 확인하였다. 그 결과, 5% 헤스페리딘이 함유된 PLGA 지지체가 가장 좋은 세포의 형태와 생체 적합성을 보여, 디스크 재생을 위한 지지체로서의 활용 가능성을 확인하였다. Hesperidin, a well-known antioxidant and anti-inflammatory agent, was smartly used for designing efficient scaffolds for disc regeneration. The scaffolds were synthesized via a salt-leaching method by adding hesperidin (0, 3, 5, & 10%) to poly(lactide-co-glycolic acid) (PLGA) followed by a cellular behaviors study by seeding annulus fibrosus (AF) cells. The cellular adhesion, proliferation and phenotypes were further evaluated by SEM, WST and RT-PCR, respectively. Additionally, AF cell behavior was studied by immunohisto-chemical staining in vivo. Results showed that PLGA scaffold with 5% hesperidin are best suited for disc regeneration, showing good cellular morphology and biocompatibility.