소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

골수염에 대한 Fucidin의 임상경험

문명상,이인주,정구효 최신의학사 1985 最新醫學 Vol.28 No.12

生産工程의 最適工程平均의 決定

李敏九,張重淳,金明洙 西原大學校 經營科學硏究所 1997 經營硏究 Vol.6 No.1

전침 자극에 의한 오디 괄약근 운동성 변화에 관한 연구

이성구,김명완,김홍자,서동환,이상수,김동일,유교상,주연호,민병일,김지훈 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

Background/Aims: This study was designed to evaluated the effects of electroacupuncture on the sphincter of Oddi (SO) motility in humans and to correlate the manometric findings with plasma cholecystokinin (CCK) levels. Methods: Eleven patients (M: F= 5: 6) who had various kinds of biliary disorders were enrolled. SO motility was monitored with a conventional low-compliant continuously perfused technique using ERCP (n=9) or percutaneous transhepatic cholangioscope (n=2). After baseline monitoring for phasic wave contractions of SO, electroacupuncture was applied at a specific acupoint, GB 34, in these 11 patients. A nonspecific acupoint 5 cm away from GB 34 was selected as a control. Manometric parameters of SO were also checked in 6 subjects during stimulation of the control acupoint. CCK plasma levels were measured during the time course of electroacupuncture stimulation. Result: All the manometric parameters including the basal pressure of SO, amplitude, frequency and duration of phasic wave contraction of SO were significantly decreased (p< 0.05) during electroacupuncture stimulation. The inhibition of SO contractility was accompanied by increased CCK plasma levels. After removal of electroacupuncture stimulation, restoration of amplitude and duration to basal condition were noted. A tendency towards the return of SO contractility was also observed in basal pressure and frequency. Stimulation of the control acupoint, however, did not affect the SO contractility and plasma CCK levels. Conclusions: Electroacupuncture stimulation of acupoint GB 34 showed reversible inhibition of SO contraction in humans. We speculate that the response of SO to electroacupuncture stimulation may be mediated by some neurohormonal mechanisms including CCK release. (Korean Journal of Gastrointestinal Motility 2000;6:44-41)

o-Tolidine에 의한 잠혈시험의 흡광광도법에 관한 연구

이명구 충북대학 대학원 1978 大學院論文集 Vol.4 No.-

본문참고

담도 및 췌장 악성종양에서의 CAl9-9의 진단적 가치에 대한 평가

명승재,최승목,김은옥,최호순,이성구,김명환,민영일,류진숙 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.3

먹파리 교상 1예

김명환,박현정,이준영,조백기,이인용,이원구 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.4

통신망의 효율적 통합관리를 위한 예비비트 활용방안

강양구,김명조,이성로,김경호 木浦大學校 應用科學硏究院 2001 應用科學硏究誌 Vol.1 No.-

In this paper, a new method for improve the performance of was presented. This method based on the use of an idle overhead in the TCP/IP protocol that used in syhchronous network structure of TMN system. Two type of modeling methodes were executed to show the performance improvements. First, it materializes the functions of the network condition scan and the remote control login. Second, this paper shows the control of a subsystem by the convert process of protocol which uses a signal conversion.

영양제로서 결정 NH₄H₂PO₄를 첨가한 피혁 공장 폐수의 생물학적 처리

전명순,임병란,최기덕,이수구 서울산업대학교 산업대학원 1993 大學院論文集 Vol.2 No.-

피혁 공장 폐수의 생물학적 처리에 있어서 유입 폐수의 급격한 부하변동, 독성물질의 유입 및 미생물의 성장에 필요한 각종 영양소의 불균형 등으로 인해 처리효율이 낮고 운전관리상 어려움이 많다. 따라서 본 연구에서는 피혁공장 폐수의 생물학적처리효율을 높이기 위하여 각종 영양제의 주입에 따른 처리효과에 관한 실험을 실시하여 적절한 영양제를 선정하고, 최적 주입량 등을 결정 하였다. 실험대상 폐수는 반월공단내의 피혁 공단 폐수로서 중화 및 응집참전 밥법으로 전처리 한 후 포기조에 유입되는 폐수를 대상으로 하였다. 영양제로는 회분식 실험에서 피혁폐수의 처리효율이 가장 우수한 것으로 나타난 결정 APM (ammonium phosphate monobasic, NH₄H₂PO₄)을 이용하였다. 연속식 활성 슬러지 공장에 있어서 폐수의 유량, 슬러지 반송율, 결정 APM 주입량 등을 변화시켜 그에 따른 처리효율에 미치는 영향을 실험 고찰하였으며, 생물 반응공학적인 반응해석을 통하여 설계에 필요한 반응상수 등을 구하였다. 연속식 활성슬러지법에서체류시간 12시간의 조건에서 영양제 미주입시 BOD 제거효율은 68%에 불과하였으나, 영양제로서 요소와 인산을 주입한 경우 73%로 증가하였으며 결정APM을 주입한 경우 90% 이상의 높은 처리율을 얻을 수 있었다. 결정 APM의 최적 주입량은 회분식 실험의 경우 300㎎/L이었으나, 연속식 실험의 경우 50㎎/L로 낮게 나타났는데, 이는 반송되는 슬러지내의 높은 인농도와 미량원소에 기인한다. 결정 APM을 50㎎/L 주입한 후 수리학적 체류시간을 6∼30 시간의 범위로 변화시켜 실험한 결과, BOD 제거효율은 체류시간 6시간에서는 67%에 불과하였으나, 12시간의 경우 93%, 30시간의 경우 98%이었다. 따라서 BOD제거효율 90%이상을 얻기 위해서는 수리학적 체류시간은 11시간 이상, F/M비는 0,65 ㎏ BOD/㎏ MLVSS·d 이하로 운전하여야 하는 것으로 나타났다. This study was performed to enhance the treatment efficiency of leather wastewater adding APM(ammonium phosphate monobasic, NH₄H₂PO₄) crystal as nutrient which contained the sufficient mineral components. The effect of APM crystal on biological treatment were studied and its optimum dosage and kinetic coefficients were determined. In the continuous activated sludge system for field wastewater of Banwol leather complex, BOD removal efficiency could be obtained over 90% with adding APM crystal, while it was 68% without nutrients and 73% with urea and phosphoric acid in the condition of HRT 12hr and SRT 3 days. The optimum dosage of APM crystal was 50㎎/L which was low compared with 300㎎/L in batch test due to recycling sludge contained high content of phosphate and mineral nutrients. In the condition of adding APM crystal 50㎎/L and hydraulic retention time in the range 6∼30hr, BOD removal efficiencies were 67% for HRT 6hr, 93% for 12hr and 98% for 30hr. In order to obtain over 90% of BOD removal efficiency, HRT should be maintained over 11 hr and F/M ratio within 0.65 ㎏ BOD/㎏ MLVSS·d.

기능성 위장관 장애에서 혈청 IL-1β, IL-2,IL-6의 변화 : 우울증 및 스트레스와의 상관성

박시성,이상신,박무인,구자영,박제민,김명정 한국정신신체의학회 2002 정신신체의학 Vol.10 No.2