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- 저자 : ( Myung Chull Rhee ) (검색결과 14 건)
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Rhee, Myung-chull The Korean Society for Integrative Biology 1998 Korean journal of biological sciences Vol.2 No.4
Various heterodimers of the thyroid hormone receptor (TR) with other nuclear hormone receptors confer a wide range of transcriptional activities on thyroid hormone response elements (TREs) in the presence of the thyroid hormone ($T_3$). The present study analyzed the potential roles of retinoid X receptor (RXR) isoforms $\alpha$ and $\beta$ in $T_3$-mediated transcription on a well characterized TRE, a direct repeat of AGGTCA separated by four nucleo-tides (DR4), using electrophoretic mobility shift assays and transient transfection in CV-1 cells. We demonstrated that RXR$\alpha$ supressed liganded $TR_{\alpha}$-induced transcription while $RXR_{\beta}$ did not although both $TR_{\alpha}/RXR_{\alpha}$ and $TR_{\alpha}/RXR_{\beta}$ heterodimers were the predominant forms bound to the TRE-DR4 in the presence of $T_3$. We further demonstrated using Scatchard analysis that the two heterodimers had similar affinities for the TRE-DR4. All these observations suggest that the TRE-DR4 accomodates different types of TR/RXR heterodimers for a more finely tuned transcriptional response to $T_3$.
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Case Report : Two Cases of Linear Alopecia on the Occipital Scalp
Chin Ho Rhee,Seong Min Kim,Myung Hwa Kim,Yong Woo Cinn,Chull Wan Ihm 대한피부과학회 2009 Annals of Dermatology Vol.21 No.2
Alopecia of a scalp shows various shapes and extents of hair loss, from a small round patch to polymorphous patches or total global alopecia. But alopecia of a linear shape is very rare. Only a few such cases have currently been reported in the medical literature. We recently had the chance to observe and treat two cases of linear alopecia that developed on the occipital scalp. The lesions themselves were like alopecia areata that shows a smooth bald area without any abnormality except the hair loss, but histopathologically, the lesions were compatible with lupus erythematosus profundus. (Ann Dermatol 21(2) 159~163, 2009)
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Spatiotemporal Expression Pattern of Chordin , a Neural Inducer in Zebrafish Embryos
Ro, Hyun Joo,Rhee, Myung Chull 한국유전학회 1997 Genes & Genomics Vol.19 No.4
Along the differentiation of a vertebrate embryo to an adult, Spemann's organizer molecules located at the blastopore lip of Xenopus gastrula turn on a series of genes triggering body pattern formation as well as neural induction. Spemann's organizer molecules are composed of secreted signal molecules such as Vg-1, Activin and Wnt, and transcriptional factors such as goosecoid, Xliml and Xnot. While the expression of goosecoid is stimulated by Activin, Goosecoid-induced gene encoding a secreted signal peptide, chordin has been cloned. Injection of chordin mRNA into ventral side of Xenopus gastrula created another complete body axis at the injected site, suggesting that Chordin is one of molecules involved in body pattern formation triggered by Spemann's orgnizer molecules. To understand molecular mechanisms wherein Chordin initiates vertebrate neural induction, spatio-temporal expression pattern of chordin gene in rebrafish embryos at three different developmental stages (one cell, cleavage and neurula) was analysed with whole-mount in situ hybridization technique. Interestingly, chordin mRNA was detected at low level from one cell and cleavage stage embryos apparently without any preponderance of spatial distribution. In contrast, it is specifically restricted to the dorsal side of neurula stage embryos with highly elevated concentration, which is consitant with previous foundings from other species. At this point, we are performing anti-sense RNA injection experiment to examine the possible function of Chordin along embryonic developmental stages.
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Identification and Expression Patterns of kif3bz during the Zebrafish Embryonic Development
Lee, A-Ram,Rhee, Myung-Chull The Korean Society for Integrative Biology 2009 Animal cells and systems Vol.13 No.4
We are reporting the identification, expression patterns, and possible biological functions of zebrafish kif3b (kif3bz) encoding 475 amino acids. Kif3Bz contains the kinesin motor domain, catalytic domain, KISc domain, and one single coiled coil domain. Phylogenetic analysis indicates that kif3bz is a highly conserved gene among the tested vertebrates. First of all, both maternal and zygotic messages of kif3bz were evenly distributed in the blastomeres at 2-cell stage. Its ubiquitous expression throughout the blastomeres continued till 40% epiboly. However, kif3bz transcripts became restricted in Kupffer's vesicle at tailbud and 6-somite stages. At 13-somite stage, kif3bz expression pattern became specific to the telencephalon, diencephalon, trigeminal placode, and somites. Such expression patterns were further intensified in the telencephalon, diencephalons, hind brain, pronephric ducts, optic vesicles, and spinal cord neurons in the 23-somite stage embryos, and last till 24 hpf. We discussed possible functions of Kif3Bz related to the vertebrate embryonic development.
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Zic3z Defines the Dorsal and Vegetal Neuroectoderm in the Zebrafish Embryonic Development
Lee, Kyu-Sun,Huh, Tae-Lin,Lee, Chang-Joong,Rhee, Myung-Chull The Korean Society for Integrative Biology 2008 Animal cells and systems Vol.12 No.1
The Zic family is a group of genes encoding zinc finger proteins that are highly expressed in the mammalian cerebellum. Zic genes are the vertebrate homologue of Drosophila pair-rule gene, odd-paired(opa), which plays important roles in the parasegmental subdivision as well as in the visceral mesoderm development of Drosophila embryos. Recent studies on human, mouse, frog, fish and ascidian Zic homologues support that Zic genes are involved in a variety of developmental processes, including neurogenesis, myogenesis, skeletal patterning, and left-right axis establishment. In an effort to explore possible functions of Zic proteins during vertebrate embryogenesis, we initially examined more detailed expression pattern of zebrafish homologue of zic3(zic3z). zic3z transcripts are detected in the neuroectoderm, neural plate, dorsal neural tube, and brain regions including eye field during early embryonic development. Marker DNA studies found that zic3z transcription is modulated by BMP, Wnt, and Nodal signals particularly in the dorsal and vegetal neuroectoderm at gastrula. Interfering with zic3z translation with zic3z-specific morpholino causes abnormal brain formation and expansion of the optic stalk cells. Retinal ganglion cells(RGCs) undergo abnormal neuronal differentiation. These findings suggest that zic3z defines the dorsal and vegetal neuroectoderm to specify brain formation and retinal neurogenesis during early embryonic development.
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So Yun Park,Yong Tae Park,Kyoon Eon Kim,Myung Chull Rhee,조희중,Dong Sun Kim 한국분자세포생물학회 2002 Molecules and cells Vol.13 No.2
The expression of the N-type voltage-gated calcium channel α1B gene is restricted to neurons by a 5′- upstream region (-3992 to -1788) that contains negative regulatory element(s) that are active in nonneuronal cells. A 39 bp DNA element, which is repeated nine times in a head-to-tail fashion, was found within the same region. To examine whether this direct repeat (DR) may function as a negatively acting cisregulatory element, several fusion plasmids, DR- 110α1BLUC (1X), DR-SV40LUC (1X, 2X), in which one or two copies of the DR fragment were subcloned upstream of the homologous and heterologous promoters, were transiently transfected into HeLa and NS20Y cells. The promoter activity of DR-110α1BLUC (1X) decreased to approximately 17% of the 110α1BLUC construct in HeLa cells. The expression of the DRSV40LUC (1X) and DR-SV40LUC (2X) plasmids was also reduced to 50 to 23% of the levels that were observed in the pGL2-Promoter in the same cells. However, no repression of the DR constructs was observed in NS20Y cells. An electrophoretic mobility shift assay showed that two DR-specific complexes were detected in HeLa cells, but not in NS20Y cells. In addition, Southwestern blotting revealed the presence of approximately 33 and 43 kDa proteins in HeLa cells. Overall, these results suggest that a 39 bp DNA element might act as repressor in non-neuron cells through the specific interactions of the DNA-proteins.
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Zebrafish 발생 과정에서 chk1 의 기능 분석
김동선,김균언,장미숙,이명철 한국유전학회 2000 Genes & Genomics Vol.22 No.3
As an initial approach to examine the function of cell cycle checkpoint kinase 1(chk1) in embryonic development, a 1.461 Kb long cDNA encoding a zebrafish chk1(Zchk1) was isolated from the 12-day-old zebrafish cDNA library. DNA sequences of the cDNA shares 87% homology with human chk7 (hchk1). However, the cDNA contains an incomplete open reading frame lacking approximately 100 by at the 3'-end region. RT-PCR analysis showed that the level of Zchk1 mRNAs was present from 1 cell stage throughout the embryonic development. Whole mount in situ hybridization demonstrated that the mRNA persisted at low level through 50% epiboly, and became restricted in the anterior part of antero-posterior axis in 3 somites, and only in the tail region of primodia 11. Inhibition of the Zchk1 expression by microinjection of Zchk1 anti-sense RNA into 1-4 cell stage embryos caused retardation of embryonic development at late somite stage, which in turn resulted in dorsally rolled tail. Taken together, these results indicate that Zchk1 may be involved in the regulation of cell cycle during embryogenesis as well as in the formation of the dorsoventral axis in the posterior region.
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