꿀벌의 日週收蜜活動에 關與하는 諸要因에 關한 硏究

李明熱,吳賢宇,崔承允,禹建錫 서울대학교농과대학농업개발연구소 1989 서울대농학연구지 Vol.14 No.1

In order to employ the honeybees (Apis mellifera) profitably as insect-pollinators, the factors governing their diurnal foraging activity, the patterns of diurnal flight activity at the hive entrance and the seasonal fluctuation of colony size were examined. The results obtained are summarized as follows. 1. There was a maximum flight activity at the hive entrance between 11h and 15h during the spring and fall but it was between 08h and 09h or 16h and 17h during the summer. This activity highly correlated with the solar energy(Mj/m²) during the spring and fall. 2. The brood area and colony weight developed to the maximum in early July but total number of flying bees at the hive entrance reached the peak early in May, and decreased in June but increased again late in July. 3. The higher the degree of flowering developed, the more active the foraging behavior. Though the degree of flowering was similar in different varieties, the foraging preference in the apple blossoms differed with varieties. 4. The amount of nectar per flower was variable with time hours, age of flowers and plant varieties. 5. The number of honeybees collecting only the nectar in the apple blossoms increased in the afternoon. Foraging behavior in the pear blossoms changed with the varieties and the numbers of honeybees collecting only the pollen (66%) or only the nectar (31%) did not change with time in the peach blossoms. 6. The diurnal foraging activities of honeybees on eight honey plants highly correlated with the solar energy but temperature, relative humidity and wind speed were not.

Detection of sacbrood virus (SBV) from the honeybee in Korea

Yong Soo Choi,Myeong Lyeol Lee,Man Young Lee,Hye Kyung Kim,Kwang Gill Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Sacbrood virus(SBV) causes a fatal disease(sacbrood) of honeybee larvae, which fail to pupate, change color and shape, and finally die. The complete nucleotide sequence of SBV has recently been determined, and with these data, we now report a Reverse Transcription-PCR(RT-PCR) test for the direct, rapid, and sensitive detection of these viruses. To detect the SBV infection in Korea, we collect beekeepers from various apiaries, which the RT-PCR technique was used. And we designed SBV specific primers in conserved region of the viral genome in the GenBank database. We confirmed the SBV amplicon using cloning and sequence. Homology between determined sequences of SBV korean strain and published virus sequences were 97% in DNA sequence, and 100% in amino acid sequence. We describe the first time that presence of sacbrood virus(SBV) in Korea honey bee colonies using RT-PCR. We also developed and validated a RT-PCR assay for the detection of SBV in Korea.

Occurrence of seven honeybee viruses in Korean apiaries

Yong Soo Choi,Myeong Lyeol Lee,Man Young Lee,Hye Kyung Kim,Kwang Gill Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Mortality of honeybees is a serious problem that beekeepers have to face periodically in Korea and worldwide. The presence of RNA viruses, in addition to other pathogens may be one of its possible causes. In this work, we detected Deformed wing virus(DWV), Israle Acute Paralysis Virus (IAPV), Black queen cell virus (BQCV), Cloudy wing virus(CWV), Kashmir bee virus(KBV), Sacbrood virus(SBV), Chronic bee paralysis virus(CBPV) in samples of korea honeybees with or without Varroa destructor and Nosema apis. The detection of viruses in all provinces, simultaneous co-infection of colonies by several viruses and the fact that 96.3% of the samples were infected with one or more virus, indicates they are widely spread in the region. Using uniplex and multiplex RT-PCR we screened honey bee colonies for the presence of several bee viruses, including DWV, IAPV, BQCV, KBV, CWV, and described the detection of mixed virus infections in bees from these colonies. Conclusively, investigated disease of the bee, and confirmed new virus that lead to bee disease, this is thought by valuable thing as data for development of beekeeping industry such as CCD(Colony Collapse Disorder)'s cause searching examination.

Antioxidant capacity of chestnut (Castanea crenata Sieb. et Zucc) honey produced in Korea

Hye-Kyung Kim,Myeong-Lyeol Lee,Man-Young Lee,Yong-Soo Choi,Nam-Suk Kim,In-Pyo Hong,Kwang Gill, Lee,Byung Rae Jin 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

This study aimed to evaluate the antioxidant activity of chestnut honey which were harvested at various areas in South Korea. First at all, we measured the total phenols content through a spectrophotometric determination with a modified Folin-Ciocalteu method and total flavonoids content determined with aluminium chloride. Total phenolic compounds was highest in Sunchang of Chestnut honey(2.21mg/ml)and flavonoids contents was also the highest in Sunchang of Chestnut honey(1.02mg/ml) than other samples. For measured the antioxidant activity of chestnut honey, we performed DPPH(2,2-diphenyl-1-picrylhydrazyl) test and FRAP(ferric reducing-antioxidant assay)test. DPPH scavenging activity highest in Sunchang of Chestnut honey more than 50% DPPH scavenging activitywhile other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 25% DPPH scavenging activity. The ferric reducing-antioxidant assay (FRAP) is based on the reduction of ferric 2,4,6-tris(2-pyridyl)-1,3,5-triazine [Fe(III)-TPTZ] by spectrophotometric analysis. Sunchang were found to have more than 532μM FRAP activity while other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 300μM FRAP activity. The results suggested that chestnut honey strong antioxidant activity and it could be utilized as a source of natural antioxidant.

Detection of chronic bee paralysis virus (CBPV) by minus-strand-specific RT-PCR

Yong Soo Choi,Myeong Lyeol Lee,Man Young Lee,Hye Kyung Kim,Kwang Gill Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Viruses of the honeybee, Apis mellifera L. are known to reside at low levels in colonies, typically showing no apparent signs of infection. Chronic paralysis virus(CBPV) is known to induce significant losses in honey bee colonies. The pathology is characterized by clusters of trembling, flightless, crawling bees and by individual bees, sometimes hairless, standing at the hive entrance. A minusstrand-specific RT-PCR was used to assess viral replication. This is the first report on the infection of CBPV in Korea. Using (-)RT-PCR, 27 apiaries in korea were screened for the honeybee viruses, with positive colonies being analysed for viral genetic diversity. We got 550-nt PCR product from CBPV genomic RNA. Nucleotide sequences were aligned to the complete CBPV genomic RNA sequence deposited in the GenBank database and was revealed 96%(AM-CBPV) identity, respectively. Sequence comparison with other CBPV and honeybee virus.

Effect of Climate Change on Life Span and Disease Susceptibility in Honey Bees, Apis mellifera L.

Myeong-Lyeol Lee,Man-young Lee,Gyu-Ho Byeon,Yong-Soo Choi,Hye-Kyung Kim 한국양봉학회 2016 한국양봉학회 학술대회 자료집 Vol.2016 No.3

Phylogeny and Diagnostic Markers of Six Tetranychus Species (Acarina: Tetranychidae) in Korea Based on the Mitochondrial Cytochrome Oxidase Subunit I

Lee, Myeong-Lyeol,Lee, Soo-Jong,Kwon, Yong-Jung Korean Society of Applied Entomology 1999 Journal of Asia-Pacific Entomology Vol.2 No.2

To understand the evolutionary relationship among six Tetranychus species in Korea, 598 nucleotides of cytochrome oxidase subunit I(COI) were sequenced and analyzed. The sequences were extremely rich in A+T(77% on average). The nucleotide differences in pairwise comparison among species ranged from 6.7% to 14.9%. Nucleotide substitutions based on all pairwise comparison showed 55% of transversions. The largest genetic distance was found between species T.viennensis and T.piercei. The inferred phylogenetic tree indicates that T. urticae, T. cinnabarinus and T.kanzawai are clustered together and showing sister-group relationship with T. truncatus at the highest boostrap support. T. viennensis showed the largest genetic distance from all other species. T.piercei, a species recently found in Korea, was also distinct fro the group composed of four species and also from T.viennensi. The restriction sites of Alu I, Dde I and Sau 3A were searched in the sequences of COI. The restriction fragment length polymorphisms (RFLPs) in COI genes and intraspecific variabilities of three restriction sites in diverse colonies of each species collected from different locations and various host plants were shown.

Distribution and control strategies of honeybee parasitic mites, Varroa destructor Anderson & Trueman, in South Korea

Myeong-Lyeol Lee,Yong Soo Choi,Won Tae Kim 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Varroa destructor Anderson & Trueman is the most injurious parasitic pest of honeybee in the world. Varroa mites had been originally external parasites of Asian honeybee (Apis cerana Fab.) in south eastern Asia. They jumped to European honeybees (Apis mellifera L.) by 1963. Since then they have killed millions of European honeybee colony, which might be susceptible to them, in Asia, Europe, America, and Africa. Also in Korea since Varroa mites were first found in 1968, they have been destructive pests in most of A. mellifera apiaries. Varroa destructor commonly infesting the European honeybees was classified in 2000 as a different species from the Varroa jacobsoni originally identified on Asian honeybees. Varroa mites not only feed the haemolymph of bees, but also introduce virulent viral diseases, and interrupt the development of bee colony. The other external parasitic mite, Tropilaelaps clarea Delfinado & Baker, which was introduced in 1994 from China, has widely spread and also brought damages on honeybees.

Characterization of Different Biotypes of Bemisia tabaci (Gennadius) (Homoptera; Aleyrodidae) in South Korea Based on 16S Ribosomal RNA Sequences

Lee, Myeong-Lyeol,De Barro, Paul J. 한국곤충학회 2000 Entomological Research Vol.30 No.2

최근 산청의 콩(Glycine max; KRG), 고양의 포인세티아(Euphorbia pulcherrima.; KRP), 진천의 장미(Rosa hybrida; KRR)에서 발견된 담배가루이 (Bemisa tabaci)의 biotype을 미토콘드리아 16S rRNA의 염기서열 분석을 통해 판별하였다. 일본, 이스라엘, 호주, 미국 등 전세계 담배가루이 7개체군의 염기서열과 비교한 결과, 포인세티아(KRP)와 장미(HRR)개체군은 미국의 아리조나, 호주, 이스라엘, 일본에 분포하는 B biotype과 동일한 염기서열을 나타내었다. 콩(KRG)에서 채집된 담배가루이는 일본의 시코쿠섬에서 발생한 담배가루이와 같은 염기서열을 보였는데, 한국과 일본의 이들 개체군은 기존의 연구결과에서 중국의 하이난, 터키, 인도북부의 개체군들과 같은 그룹에 위치하는 non-B biotype이었다. 일본 이시가키섬의 담배가루이는 타 개체군들과 다른 제3의 유전형을 보유하였다. 16S rRNA의 염기서열에 의한 이들 전체 11개 개체군들의 상호간 유전적 유사도와 계통 유연관계가 추정되었다 The identity of biotypes of Bemisia tabaci, recently found in Korea, were determined using a partial sequence of its mitochondrial 16S rRNA. The samples of B. tabaci collected from Gtycine max (KRG), Euphorbia pulchenima (KRP), and Rosa hybpida (KBR) were compared with seven previously identified populations from Japan, Israel, Australia, and the USA. The sequences from both KRP and KRR were identical to those of B biotypes distributed in Arizona, Australia, Israel, and Japan. The sequence obtained from KRG was identical to that obtained from a population on Shikoku Island, Japan. These two populations are non-B biotype and in an earlier study have been shown to belong to the same clade as populations from Hainan, Turkey and northern India. A third distinct genotype was also found in a population from Ishigaki Island, Japan. The genetic distance in pairwise comparison and a phylogenetic tree inferred from 16S rRNA sequences are shown.

Selection of Triple Crossed Honeybee (Apis mellifera L.) Enhanced Honey Production

Myeong-lyeol Lee,Man-young Lee,Yong-soo Choi,Hye-kyung Kim,In-suk Kim,Hyo-yeol Choi 한국양봉학회 2016 한국양봉학회 학술대회 자료집 Vol.2016 No.3