독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

부산시 범죄피해조사 1996/97

文章一,金慶和 동아대학교 법학연구소 1997 東亞法學 Vol.- No.23

고등학교 야구선수들의 경쟁상태불안에 관한 연구

김상규,김성문,김경석,권판근 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.3

The Purpose of this study is to investigate the surbordinate elements of the competition state anxiety-cognitive state anxiety, somatic state anxiety, and state self-confidence- before the game according to their grades, positions and rols, and to present some useful guiding principles of effective coaching and the basic data of conduct guide to improve the performance ability and control competition anxiety of high school baseball players. A total of 410 high school baseball player who participated in the 57th Chungryonggi National High School Baseball Tournament(2002. 5. 1-5. 8) took part in the CSAI-Ⅱ. The collected data were analyzed with descriptive statistics and one-way ANOVA. Result were as followings: First, in the analysis of the competition state anxiety according to the grade, there isn't any significant difference in the somatic state anxiety statistically. However, there are significant difference in the cognitive state anxiety and state self-confidence statistically (p<.05). Second, in the analysis of the competition state anxiety according to the position, there isn't any significant difference in the cognitive state anxiety and state self-confidence statistically(p<.05). Third, in the analysis of the competition state anxiety according to the game performance ability, there isn't any significant difference in the cognitive state anxiety and somatic state anxiety statistically. However, there are significant difference in the state self-confidence statistically(p<.001). Forth, in the analysis of the competition state anxiety according to the roles, there isn't any significant difference in the cognitive state anxiety, somatic state anxiety, and state self-confidence statistically.

[논문]병렬 유전 알고리즘을 이용한 발전기 기동정지계획

김형수,문경준,박준호 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

본 논문에서는 병렬 유전 알고리즘을 이용한 발전기 기동정지계획을 제안한다. 최소 기동 및 정지 시간 등과 같은 다양한 발전기의 제약조건을 만족시키면서 발전기 기동정지계획을 수립하는 문제는 비선형적이며 많은 국부해가 존재하므로 최적해를 탐색하는데 많은 시간이 소요된다. 이러한 문제점을 해결하기 위해 16개 의 프로세서를 가진 병렬 시스템과 이를 이용한 병렬 유전 알고리즘을 제안하였으며, 제안한 방법의 효용성을 검토하기위해 10기 및 26기의 전력계통에 적용하여 시율레이션을 시행하였다. 시율레이션 결과 기존의 방법에 비해 탐색속도를 개선하였고 우수한 해를 구할 수 있었다.

한국인 태아 및 사산아에서 얼굴신경에 대한 형태학적 연구

강양수,김종중,김복,정상봉,문영민,설경수,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

It is critical that the accurate anatomy of the facial nerve IS known for uncomplicated successful facial surgery. Such an operation is hazardous because of the intimate relationship between the facial nerve and the expressional muscles. It is hoped that such basic information will be helpful to those who treat conditions involving the facial region. In order to define the detail of this relationship, many studies have been undertaken on dissected adult specimens. This study was based on Korean fetus and stillborn infant cadaveric dissections, and describes anatomical variations of the peripheral branches of the facial nerve that pose a potential danger in a number of surgical procedure on the face. The branching patterns were classified into six types: the frequencies of occurrence were: type I, 4.9%; type II, 24.4%; type III, 34.1%; type IV, 19.5%; type V, 12.2%; and type VI, 4.9%. Types II, III and IV together accounted for almost 80% of the specimens. In the discussion we compare the results of the present study with those of others and try to clarify the anatomical characteristics of the facial nerve in infants, which demand that special attention be paid to the avoidance of injuries during facial surgery.

유전알고리즘을 이용한 모델추종형 퍼지제어기 설계

황기현,문경준,김문수,원태현 동의공업대학 1998 論文集 Vol.24 No.1

In this paper, a reference model following control system using a fuzzy logic controller (FLC) is proposed. A reference model whose response does not overshoot and has a fast rise time is designed. A FLC is designed to follow as close as possible the response of the reference model. The proposed design method has the robustness and the optimal tracking property under modeling error, disturbance and parameter perturbations. The proposed designing method is compared using a PD controller throughout the experiment. The effectiveness of the proposed designing method is verified through computer simulation and experiments for the second-order and the first-order reference model.

'유아교육론'강좌의 성격 탐구

윤기영,표경남,류문숙,김현식 서원대학교 교육대학원 2003 교육논총 Vol.7 No.-

The purpose of this study is to provide desirable directions for early childhood teacher education. To serve this purpose, the character on 'early childhood education theories' lectures are studied and examine the actual conditions of the 'subject education theories' lectures in university or college early childhood education Dept. And analysis the theories related subject matter. The 'early childhood education theories' lecture was characterized are as followers ; First, 'early childhood education theories' lecture means whole child education as not subject matter education. Second, 'early childhood education theories' lecture means school level education as primary(elementary) school level education. Third, 'early childhood education theories' lecture means generate subject education as like integrated curriculum, not like seperate subject education curriculum.

만경강 유역내 하천의 특성별 영향 평가

윤순강,이종식,정구복,김민경,김선종,고문환,엄기철 한국환경농학회 2002 한국환경농학회지 Vol.21 No.4

본 연구는 만경강 유역의 수질 보전을 위한 기초자료를 얻기 위하여 만경강 본류와 이에 유입되는 지천을 선정하여 2001년 5월부터 11월까지 매월 수질 모니터링을 실시하였다. 만경강 유역내 본류 및 지천의 유기물과 영양염류 함량을 조사한 결과, 본류의 T-N은 3.78~12.68 ㎎/L였으며, 지천중 축산폐수가 유입되는 익산천의 농도가 가장 높았다. 본류의 T-P의 BOD는 각각 0.043~0.864 ㎎/L와 2.59~13.29 ㎎/L였으며, 지천중 익산천이 가장 높았다. 본류의 COD는 12.9~119.5 mg/L였으며, 지천중 도시생활하수가 유입되는 추천이 가장 높았다. 만경강 유역 수질저하의 주 원인은 축산폐수였으며, 도시생활하수가 그 다음으로 중요한 원인으로 평가되었다. 만경강 본류의 T-N과 T-P는 하천의 유량이 증가하는 홍수기인 7~8월보다 장마선 갈수기인 5~6월에 높았고, COD는 장마전 갈수기보다 홍수기에 높았으며 장마가 완전히 끝난 영농후 갈수기인 9~11월에도 수질저하가 지속되었다. 만경강에 유입되는 지천중 전반적으로 수질오염이 심각한 익산천과 추천은 영농후 갈수기에 수질이 가장 저하되었고 상대적으로 홍수기에는 양호하였다. A survey on four tributaries along with Mankyeong River was carried out to get the information for the water quality improvement and control. Typical paddy farming were major agricultural practices in Kosancheon and Soyangcheon. Iksancheon was livestock raising watershed. Chucheon was urban watershed. Water quality in six sites of main stream and four sites of tributaries in Mankyeong River were investigated from May to August in 2001. The concentration of nutrients in main stream of Mankyeong River were in the range of 3.78~12.68 mg/L for total nitrogen, 0.043~0.864 mg/L for total phosphorus, 2.59~13.29 mg/L for BOD and 12.9~119.5 mg/L for COD, respectively. Water quality of Mankyeong River mostly exceeded the standard water quality criteria of Korea. Major causes of water pollution were evaluated as sewage of swine and urban area. Among the four tributaries, water quality in agricultural practices, Kosancheon and Soyangcheon was relatively less polluted. While, the highest level of water pollution measured in Iksancheon was due to livestock The water quality of Iksancheon and Chucheon was generally more polluted in the dry period than in rainy period.

친환경농업 시범마을에 대한 환경영향평가

이남종,고병구,노기안,한민수,김민경,곽한강,박문희 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

1999년부터 2001년까지 3년간 친환경농업 시범마을에 적용되고 있는 INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) 실천에 따른 추진효과를 분석하기 위하여 시범마을로 지정된 옥천과 양평지역의 농가 포장에서 시비량, 양분유실 및 생태계 변화 등을 조사 분석하였다. 3요소 시비량은 토양검정시비로 인하여 농가관행시비와 비교하여 28.6~39.4% 절감할 수 있었으며, 쌀 수량도 시비량이 비교적 적은 토양검정 시비구에서 3~10% 증수하였다. 영농기간동안 토양 침투수중 NO₃-N 농도는 농가관행 시비구 2.9 ㎎/L에 비하여 토양검정 시비구는 1.5 ㎎/L로 감소하였으며, 시범마을 논으로 유입되는 관개수의 COD, NH₄-N, NO₃-N 농도는 각각 15.0, 0.67, 1.39 ㎎/L, 시범마을 논에서 배출된 용수는 각각 12.4, 0.29, 2.42 ㎎g/L로 감소되는 경향이었다. 처리별 수서곤충, 환형동물, 패류 및 감각류 등의 개체수는 농가관행구 100에 대하여 토양검정 시비구 293, 토양검정과 개량제 혼용구 276, 무비구 90으로 토양검정 시비구에서 많았으며 수서 무척추 동물은 관행농업을 수행하고 있는 지역을 100으로 볼 때 친환경농업마을은 242와 443으로 INM과 IPM을 실천하는 시범마을에서 생물종이 다양하고 그 개체수가 많은 것으로 나타났다. 친환경농업 시범마을 조성 후 작물 추천시비량 준수와 적기 예찰을 통하여 필요이상 사용되는 비료와 농약을 절감하여 생태계의 개선과 토양이 건전화된 것으로 평가되었다. In order to preserve the soundness of ago-ecosystem and products safety in agriculture, this study was evaluated environmental impact in relation to the INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) at paddy fields at Ok-Chun and Yang-Pyung region. By introduction of INM with the application of BB(bulk-blanded) fertilizer based on soil analysis and EM, the application rate of fertilizer was reduced to about 28.6-39.4% and the yield of brown rice was increased to about 3-10% compared to conventional practices. The concentrations of COD, NH4-N, and NO3-N in irrigation water flowed to the environmental-friendly agriculture practices were 15.0, 0.67, and 1.39 ㎎/L, respectively. The concentrations of COD, NH₄-N, and NO₃-N from paddy fields in drainage water were 12.4, 0.29, and 2.42 ㎎/L, respectively. The total number of the freshwater invertebrates was higher in field treated with fektilhtion by prescription with soil testing. Also, the population density of aquatic insects was higher than the other fields at both demonstration villages. In conclusion, it was possible to reduce the amount of fertilizer and agricultural chemicals application, and increase the yield of rice by application of the environmental-friendly agriculture practices.

A New Lodging Tolerant and High-Yielding Glutinous Rice Cultivar "Dongjinchalbyeo"

Mun Sik Shin,Hyun Tak Shin,Jae Kil Lee,Kyu Seong Lee,Bo Kyeong Kim,Jae Kwuon Ko,Tae Hwan Noh,Ki Yong Ha,Young Doo Kim,Bo Kap Yang,Yang Ho Oh,Chong Seok Kim,Seung Yeob LeeHyun 한국육종학회 2003 한국육종학회지 Vol.35 No.4

“Dongjinchalbyeo” is a new japonica glutinous rice cultivar developed from a cross between Milyang95 and SR11155-4-2/Toyonishiki by the rice breeding team of the National Honam Agricultural Experiment Station (NHAES), RDA, in 1998. This cultivar has a sho