Regulation of UVB-Induced IL-8 and MCP-1 Production in Skin Keratinocytes by Increasing Vitamin C Uptake via the Redistribution of SVCT-1 from the Cytosol to the Membrane

Kang, Jae Seung,Kim, Ha Na,Jung, Da Jung,Kim, Jee Eun,Mun, Ga Hee,Kim, Yeong Seok,Cho, Daeho,Shin, Dong Hoon,Hwang, Young-Il,Lee, Wang Jae Williams & Wilkins 2007 The Journal of investigative dermatology Vol.127 No.3

It is well known that UVB (290–320 nm) induces inflammation in skin by the transcription and release of cytokines and chemokines from skin keratinocytes. In addition, it is considered that intracellular reactive oxygen species (ROS) plays an important role in UVB-induced inflammatory response in the skin. Therefore, we investigated the effect of vitamin C, a potent antioxidant, on the regulation of UVB-induced skin inflammation via the modulation of chemokines production. Vitamin C uptake into keratinocytes is increased by UVB irradiation in a time- and dose-dependent manner through the translocation of sodium-dependent vitamin C transporter-1 (SVCT-1), a vitamin C-specific transporter, from the cytosol to the membrane. To evaluate the effect of vitamin C on the chemokine mRNA expression, we performed RNase protection assay. As a result, there was a remarkable change in chemokine mRNA expression, especially IL-8 and monocyte chemoattractant protein (MCP)-1 expression. In addition, increased IL-8 and MCP-1 mRNA expressions were suppressed by vitamin C treatment. We also confirmed the results of protein levels measured by ELISA. Taken together, vitamin C uptake is increased in UVB-irradiated keratinocytes through the translocation of SVCT-1 and regulates inflammatory response in the skin via the downregulation of IL-8 and MCP-1 production.Journal of Investigative Dermatology (2007) 127, 698–706. doi:10.1038/sj.jid.5700572; published online 28 September 2006

골수이식 후 사이토카인과 골교체 생화학적표지자의 변화 및 상관관계

민우성,강무일,한제호,강성구,오기원,이원영,김혜수,문성대,손현식,신완식,김춘추,윤건호,차봉연,이광우,손호영 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.1

Background : Loss of bone mass is usually detected after BMT. The causes of bone loss are related with gonadal dysfunction and immunosuppressants. Cytokines, especially IL-6, play an important role in the pathogenesis of postmenopausal osteoporosis. However, the pathogenetic role of cytokines in post-BMT bone loss is unknown and data on the changes of cytokines in accordance with bone turnover markers are scarce. The aim of this study is to assess the relationship of bone turnover markers and cytokines of peripheral blood and bone marrow before and after allogeneic BMT. Methods : This prospective study included two analyses. The first was a study of 46 BMT recipients, examining the relationship between bone turnover markers and cytokines of serum which were measured before and 1, 2, 3, 4 week and 3 months after BMT. The second was a study of 14 BMT patients, measuring bone marrow plasma cytokines such as IL-6 and TNF-? at post-BMT 3 week and bone turnover marker at the same time to assess the relationship beween two parameters. Results : Serum ICTP, bone resorption marker, increased progressively until 4 weeks (peak) after BMT and then decreased thereafter. Serum osteocalcin, bone formation marker, decreased progressively until 3 weeks after BMT and then increased thereafter. There was positive correlation between serum ICTP and bone marrow IL-6 levels at the post-BMT 3 week with a statistical significance, but the correlation between bone turnover markers and bone marrow TNF-? or peripheral blood cytokines was not found. Conclusion : Our data suggest that the progressive increase of bone resorption after BMT is related with the increase of bone marrow IL-6, which is a potent stimulator of bone resorption in vivo(J Kor Soc Endocrinol 15:85-96, 2000).

이미지 검색을 위한 관심영역의 요소추출과 데이터 변환 연구

강문주,양동일,김덕은,최형진 강원대학교 정보통신연구소 2006 정보통신논문지 Vol.10 No.-

In this paper we designed the content-based image system. It discovers the characteristic value of color area for the existing image, when any user establishes the special interesting region for the question images. And we designed the system to be able to search the similar image of the interesting region, compared with the table that saved the characteristic value of moment which is unchangeable for the size and the rotation of form. In this paper, we suggest the image search model based on neural network. This is able to seek for the aimed image through the feedback to search the nonlinear relations by the method of searching content-based image using SOM neural network. We make use of the Precision and the Recall that are used for the system evaluation using the similar matching method in general. As a result of the evaluation of the content-based image system, we can reduce the time and memory to find out the feature value of the image that is meaningless in the general images.

이미지 검색을 위한 관심영역의 오소추출과 데이터 변환 연구

강문주,양동일,김덕은,최형진 강원대학교 정보통신연구소 2006 정보통신논문지 Vol.10 No.-

In this paper we designed the content-based image system. It discovers the characteristic value of color area for the existing image, when any user establishes the special interesting region for the question images. And we designed the system to be able to search the similar image of the interesting region, compared with the table that saved the characteristic value of moment which is unchangeable for the size and the rotation of from. In this paper, we suggest th image search model based on neural network. This is able to seek for the aimed image through the feedback to search the nonlinear relations by th method of searching content-based image using SOM neural network. We make use of the Precision and the recall that are used for the system evaluation using the similar matching method in general. As a result of the evaluation of the content-based image system, we can reduce the time and memory to find out the feature value of the image that is meaningless in the general images.

플루오르토파즈와 휘스커 뮬라이트의 제조와 특성평가

심일용,이홍림,문종수,강종봉 경남대학교 신소재연구소 2002 신소재연구 Vol.14 No.-

본 연구에서는 1200℃의 저온에서 침상형 뮬라이트를 합성하고, 이러한 침상형 뮬라이트를 생성하는 메카니즘을 관찰함과 동시에 메카니즘을 활용한 제어된 미세구조를 가진 뮬라이트를 합성하고자 하였다. Al원으로 사용되는 원료로서는 Al(OH)_3를 사용한 경우가 Al_2O_3를 사용한 결과보다 더욱 발달된 휘스커상의 뮬라이트를 얻을 수 있었다. 각 온도구간에서 열처리한 시편은 미세구조 관찰과 상분석을 통하여 플루오르토파즈와 휘스커상의 뮬라이트의 생성을 관찰하였으며, 토파즈상을 거쳐간 휘스커 뮬라이트의 생성은 하나의 토파즈 상에서 여러 방향으로 휘스커상의 뮬라이트가 성장되는 것을 관찰할 수 있었다. 1200℃에서 생성된 휘스커상의 뮬라이트는 [001] 방향으로 성장하였으며, (110)면의 면간거리는 5.34Å이었다. 과량의 Al(OH)_3를 참가하였을 경우 기존의 8:7:4 원료조합보다 11:7:4의 경우에 조대한 단일상의 휘스커 뮬라이트를 관찰할 수 있었으나, 그 이상의 Al(OH)_3의 첨가는 미반응 Al_2O_3로 인하여 단일상의 뮬라이트를 얻지 못하였다. Whiskered mullites were synthesized at relatively low temperature of 1200℃, and mullite forming mechanism was studied in this experiment. Al(OH)_3 as a raw materials was better in getting whiskered mullite than Al_2O_3. Microstructure and phase analysis were done for the specimens heat treated at various temperatures. It was found that the synthesized mullite had grown to [001] direction, and that the distance of (110) plane was 5.34Å. Al(OH)_3 addition resulted in larger mullite whisker than normal 8:7:4 ratio, but excessive Al(OH)_3 didn't affect the growth of mullite whisker.

핵간안근마비를 보인 중추신경계 크립토콕쿠스병 1례

이지영,김재일,김기선,강문철,이창민,양대웅,이근호,김상준 대한신경과학회 1997 대한신경과학회지 Vol.15 No.5

효소형 분석에 의한 국내 등줄쥐(Apodemus agrarius coreae)와 흰넓적다리붉은쥐(Apodemus peninsulae peninsulae)의 특성 조사

김희선,강문일,변정옥,한동운 한국수의공중보건학회 2002 예방수의학회지 Vol.26 No.4

경기, 강원, 충청, 경상 그리고 전라지역 에서 225마리의 들쥐를 포획하였더니 Apodemus agrarius coreae가 200마리로 전국적인 분포를 보였고, Apodemus peninsulae peninsulae는 25마리로 강원, 경상 및 전라지역에서만 포획되었다. 전기영동법을 이용하여 이들 두 종에 대한 간장 조직에서 조사하였던 G6PDH, ADA, PEP-A, PEP-B, AAT, SOD, LDH, MDH, PNP, GAPDH 등 10종 모두가 검출되었다. 등줄쥐에서는 전기영동분석 결과 32종의 표현형이, 흰넓적다리붉은쥐에서 8가지가 검출되었다. 두 종의 locus의 평균 유전학적인 다양성은 0.130으로 나타났고, 유전학적인 차이는 0.100~0.600으로 상대적인 차이를 보였으며, 등줄쥐는 0.150~0.398사이에 네 개의 집단으로 구분되었던 반면 흰넓적다리붉은쥐는 0.100~0.275의 차이로 확인되었다. 경기지역의 안성, 여주 그리고 전라지역의 완주에서 포획된 등줄쥐를 제외하고는 지역에 따라서 6~11개의 조직내 효소 표현형이 중복되어 있었다. 흰넓적다리붉은쥐 역시 한 가지 이상의 표현형이 있었고 이들은 지역적인 차이를 보였으며, 등줄쥐와 조직내 효소형과 중복되어 있었다. Two hundred of Apodemus agarius coreae and twenty five of Apodemus peninsulae peninsulae were caught from Kyonggi, Kangwon, Chungchong, Jeolla and Kyongssang provinces across the country. Isoenzyme patterns were analyzed by gel electrophoresis with liver homogenates of A. a. coreae and A. p. peninsulae. Using 10 enzyme systems, 32 electrophoretic types (ETs) were identified from two species of Apodemus including 8 ETs from A. p. peninsulae with genetic diversity per locus between 0.00 and 0.54 (mean 0.13). Genetic distances among ETs of A. a. coreae and A. p. peninsulae was between 0.100 and 0.600. Thirty two ETs of both species of Apodemus were classified to four major groups with genetic distance from 0.150 to 0.398. Eight ETs of A. p. peninsulae were grouped into four subgroups with genetic distance from 0.100 to 0.275. All ETs of A. p. peninsulae were belong to those of A. a. coreae with enzymatic variation. There was only one ET in A. a. coreae from Ansong an Yoju counties in Kyonggi and Wanju county in Jeolla province. However, all samples collected from the rest were identified to have 6 to 11 ETs on local enzymatic variance. Enzymatic overlap between both species was confirmed with at least one ET depending on the sites of collection.

Platelet-Rich Plasma (PRP)가 토끼 척골의 분절 결손의 치료로 이식된 동종 장관골의 골유합에 미치는 효과

송주현,정진영,강용구,이한용,손문익,서상일 대한골절학회 2003 대한골절학회지 Vol.16 No.3

목적: 골종양 환자나 외상, 골수염, 인공관절 치환술 등의 환자 등의 환자 치료에 많이 사용하는 장관골의 동종 이식에 대한 PRP (Platelet-Rich Plasma)의 효과를 토끼를 이용한 실험으로 알아보고자 하였다. 대상 및 방법: 20마리의 토끼를 대상으로 각각에 일련번호를 붙이고, 각 토끼의 양측 척골에 약 2㎝ 길이의 골편을 얻어 장관골 결손을 만들고 동시에 동종 장관골을 얻어, 홀수 토끼의 척골 골편을 짝수 토끼의 골결손 분위에 이식하고, 반대로 짝수 토끼의 척골 골편을 홀수 토끼의 골결손 분위에 이식하였다. 그 후 각 토끼의 좌측 척골 이식 부위에는 아무런 처치를 하지 않고 대조군으로 삼고, 우측 척골 이식부에는 PRP 0.7cc를 처치하여 실험군으로 삼았다. 술 후 0, 4, 8, 12주에 골이식부에 대한 방사선 촬영을 통하여 골유합 성적을 평가 비교하였다. 결과: 실험군의 척골 분절 결손부에서는 대조군에서보다 동종 분절골 이식부의 골유합이 방사선학적으로 향상된 것을 관찰 할 수 있었다. 결론: 동종 장관골 분절 이식시 PRP를 병용하여 사용할 경우 더욱 효과적인 신생조직 형성을 유도할 수 있으며, 임상에도 유용하게 적용할 수 있으리라 사료된다. Purpose: The purpose of this study was to evaluate the effect of Platelet-Rich Plasma(PRP) on the healing of the allograft for the treatment of the segmental bone defect of the ulna in Rabbits. Materials and Methods: About 2 ㎝-sized segmental bone defects were created on both ulna of twenty rabbits. The rabbits were divided into two groups, even and odd number groups after numbering them from 1 to 20. The segmental bone from the odd numbered animal was transplanted to the even numbered animal, and the even numbered to the odd numbered. The left side of the ulna was grafted with a segmental allograft only. The right side of the ulna was grafted with a segmental allograft and 0.7cc of PRP. Radiographs obtained at 0, 4, 8, and 12 weeks postoperatively were graded for radiologic union. Result: The use of the combination of PRP and segmental allograft demonstrated improved healing on radiographic study compared with that demonstrated after use of allograft alone. Conclusion: The results of the study suggests that the use of the combination of PRP and segmental allograft can be considered as an alternative method to manage the segmental defect of the long bone.

젖소 말초혈액 림프구로부터 소백혈병 바이러스 배양 및 전자현미경적 관찰

윤순식,박중원,변재원,강문일,유한상,한홍율 韓國電子顯微鏡學會 2005 Applied microscopy Vol.35 No.1

국내 젖소의 54.2%가 BLV에 감염되어 있지만 현재까지 국내에서는 소백혈병 바이러스 (BLV) 입자를 확인한 연구 보고가 없기 때문에 BLV 항체 양성 소의 말초혈액림프구를 배양, BLV를 발현시켜 전자현미경으로 바이러스 입자를 검출하였고 배양조건에 따른 바이러스 발현율 및 발현 시간을 비교하였다. 검사 결과 전형적인 C-형 바이러스를 확인할 수 있었으며 BLV 단크론 항체를 이용한 면역염색결과 BLV 항원 양성으로 확인되었다. BLV 는 대부분 세포 외부에 분포하고 있었으며 세포질 막에서 생성, 발아되어 나오는 것도 관찰되었다. 전체 바이러스의 크기는 90~100 nm였으며 nucleocapsid는 40~60nm였다. 소태아혈청 (FBS)과 T- 및 B-림프구 분열촉진물질(mitogen)을 각각 첨가하여 배양한 결과 두 군 모두에서 BLV 발현이 확인되었다. Lipopolysaccharide 첨가군은 배양 12시간, Conconavalin A 첨가군은 배양 24시간에 각각 림프구의 10%에서 바이러스가 관찰되었다. 또한 FBS만 첨가한 군과 FBS와 mitogen을 모두 첨가하지 않은 군에서도 관찰되었으나 바이러스의 수는 적었다. 본 연구에서 확립된 BLV 배양 기법을 활용하면 BLV에 감염된 소 중 바이러스를 발현하는 소, 즉 전파능이 있는 개체를 찾아내어 우선적으로 도태할 수 있기 때문에 BLV 감염으로 인한 피해를 막는데 효과적으로 이용될 수 있을 것으로 사료된다. Many studies have been performed on the bovine leukemia virus (BLV) since bovine leukosis had been reported in 1968 in Korea. However, there was no report on the ultrastructural examination of BLV. An attempt to detect C-type viral particles in the cultured peripheral blood lymphocytes of Holstein-Friesian dairy cattle, was made to determine whether in vitro viral expression might be used as a reliable method to identify the cow which is likely to transmit BLV. In transmission electron microscopic (TEM) examination, the virus particles were found predominantly outside of the lymphocytes even though a few particles were also observed within the membrane bound cytoplasmic vacuoles. All of them were C-type particles consisting of a central, electron-dense core separated by a clear area from a limiting envelope with a unit membrane structure. Virus particles were easily detected in the lymphocyte which was cultured with medium supplemented with either T-lymphocyte mitogen (conconavalin A) or B-lymphocyte mitogen (lipopolysaccharide). Identical viral particles, although fewer, were also consistently present in the lymphocytes cultured with medium which was containing foetal bovine serum (FBS) only and which was containing neither FBS or mitogen. By contrast, no virus particle was detected in extensive examination of lymphocytes before culture. In conclusion, the BLV cultivation and detection methods established in this study could be used as a tool to identify and eliminate the cattle which can transmit the BLV.

Proceedings of The 2(nd) Asian Society of Veterinary Pathology Symposium and 2005 Annual Meeting of The Korean Society of Veterinary Pathology : Opening Ceremony ; Greetings 2

( Mun Il Kang ) 한국수의병리학회 2005 한국수의병리학회지 Vol.9 No.2