숯첨가 사료투여가 넙치의 면역반응에 미치는 영향

권문경,이윤호,박상언,김봉석,박수일 한국어병학회 2002 한국어병학회지 Vol.15 No.1

숯첨가 사료 투여가 넙치에 미치는 영향을 알기 위하여, 넙치에 숯 1%, 3%, 5% 첨가사료를 8주간 투여하면서 성장, 혈액성분, 비특이적면역능을 분석하였으며 어병세균인 Edwardsiella tarda 균의 공격 시험에 대한 방어능을 조사하였다. 숯 첨가 사료 투여는 넙치의 성장과 라이소자임 활성에서는 유의적인 차이를 나타내지 못했지만, 5% 숯첨가구에서 혈액 중의 total protein과 cholesterol의 농도가 대조구에 비하여 낮게 나타났다. 그리고, 숯 3% 첨가구에서 두신 식세포의 활성 및 병원성 세균인 E. tarda에 대한 방어능도 가장 높게 나타났다. The charcoal effects on the immune responses of flounder, Paralichthys olivaceus were studied. Fish were fed an experimental diet supplemented with 1%, 3% or 5% of charcoal to a commercial diet. Several factors such as weight gain, hematological parameters, nonspecific immune responses and resistance against Edwardsiella tarda were evaluated 4 and 8 weeks after the administraion. The changes of body weight and lysozyme activities were not signigicant among tested groups. But cholesterol and total protein concentration in the blood were significantly lower in the 5% supplemented group than in the control. The production of superoxide by macrophage from the head kidney, examined by NBT reduction, was significantly higher in the 3% charcoal supplemented groups than in the control. The survival rate after an artificial challenge with 1×10^exp(7) cells of E. tarda per fish was significantly the highest in the 3% charcoal supplemented group.

넙치, Paralichthys olivaceus의 수온 자극 스트레스에 대한 사료첨가제 투여 효과

권문경,박상언,방종득,조병열,이상민,박수일 한국어병학회 2003 한국어병학회지 Vol.16 No.3

본 연구는 넙치의 수온 스트레스에 대한 사료첨가제 (미역 5%, 미역 10%, 어보산 0.5%, 고추냉이 잎 2%, 고추냉이 줄기 2%)의 영향을 혈액학적, 면역학적 측면과 Edwardsiella tanda를 사용하여 질병에 대한 저항성으로서 평가하였다. 혈장의 글루코즈와 콜티졸 농도는 1차 수온 변동 후 대조구에 비하여 미역 5%와 고추냉이잎 2% 첨가구에서 유의적으로 낮게 나타났다(P〈0.05). 1차 수온 변동 후 혈장 라이소자임 활성과 E. tarda의 공격 시험 후 생존율은 미역 5%첨가구에서 대조구에 비하여 유의적으로 높게 나타났다(P〈0.05). 본 실험 결과 미역 5% 첨가 사료 투여는 넙치의 스트레스 상태에서 질병 감염에 대한 저항성을 높일 수 있을 것으로 사료된다. The effect of each dietary supplements (Undaria 5%, Undaria l0%, Obosan 0.5%, Wasabi leaf 2%, Wasabi stem 2%) on the water temperature fluctuation in juvenile olive flounder (Puralichthys olivaceus) was investigated. The response to stress was assessed in terms of effects on haematological and immunological, and resistance against Edwurdsiella tarda infection. Plasma glucose and cortisol levels were significantly lower in 5% undaria and 2% wasabi leaf supplement groups than the controls after the first change of water temperature (P<0.05). The plasma lysozyme activities and the survival rates from E. tarda infection were significantly higher in the 5% undaria supplement groups than the control (P<0.05). These results suggest that the 5% undaria supplement seems to be contributable to the increased disease resistance on olive flounder.

Molecular cloning and expression analysis of a C-type Lectin in the rock bream Oplegnathus fasciatus

( Mun Gyeong Kwon ),( Ju Won Kim ),( Myoung Ae Park ),( Jee Youn Hwang ),( Hyung Jun Park ),( Chan Il Park ) 한국어병학회 2012 한국어병학회지 Vol.25 No.1

C-type lectins are crucial for pathogen recognition, innate immunity, and cell-cell interactions. In this study, a C-type lectin gene was cloned from the rock bream. The full-length RbCTL cDNA was 729 bp with a 429 bp ORF encoding a 164-residue protein. The deduced amino acid sequence of RbCTL had all of the conserved features crucial for its fundamental structure, including the four cysteine residues involved in sulfide bridge formation and potential Ca2+/carbohydrate-binding sites. RbCTL contains a signal peptide one single carbohydrate recognition domain. It showed 29.4% similarity to the C-type lectin of rainbow trout. RbCTL mRNA was predominately expressed in gill and head-kidney tissue and expressed less in peripheral blood leukocytes, trunk-kidney, spleen, liver, intestine and muscle. Expression of RbCTL was differentially upregulated in rock bream stimulated with LPS, Con A/PMA and poly I:C.

The Effects of Supplementary diets on the Water Temperature Stress in Olive Flou

Kwon, Mun-Gyeong 부경대학교 수산과학연구소 2004 硏究業績集 Vol.28 No.-

Molecular identification and expression analysis of bactericidal permeability-increasing protein/ LPS-binding protein (BPI/LBP) from Black rockfish Sebastes schlegeli

Kwon, Mun-Gyeong,Kim, Ju-Won,Park, Myoung-Ae,Hwang, Jee-Youn,Park, Hyung-Jun,Baeck, Gun-Wook,Park, Chan-Il The Korean Society of Fish Pathology 2010 한국어병학회지 Vol.23 No.3

Bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP) are important components of the mammalian innate defence system against Gram-negative infections. The BPI/LBP cDNA was identified from the black rockfish ConA/PMA or LPS stimulated leukocyte cDNA library. The full-length BR-BPI/LBP cDNA was 2118 bp long and contained an open reading frame (ORF) of 1422 bp that encoded 473 amino-acid residues. The 5' UTR had a length of 57 bp, and the 3' UTR 639 bp. The molecular weight and theoretical isoelectric point (pI) values were calculated 51.4 kDa and 9.72, respectively. Compared with other known BPI or BPI/LBP peptide sequences, the most conserved regions of the black rockfish BPI/LBP peptide were found to be the BPI1 N-terminal, BPI2 C-terminal domains and a LPS binding domain. Phylogenetic analysis based on the deduced amino acid sequence revealed a homologous relationship between the BPI/LBP sequence of black rockfish and that of other teleosts. The black rockfish BPI/LBP gene was predominantly expressed in the PBLs, head kidney, trunk kidney and spleen. The expression of the black rockfish BPI/LBP molecule was induced in the peripheral blood leukocytes (PBLs) from 1 to 24 h following LPS stimulation, with a peak at 12 h post-stimulation.

Molecular identification and expression analysis of bactericidal permeability-increasing protein/ LPS-binding protein (BPI/LBP) from black rockfish Sebastes schlegeli

( Mun Gyeong Kwon ),( Ju Won Kim ),( Myoung Ae Park ),( Jee Youn Hwang ),( Hyung Jun Park ),( Gun Wook Baeck ),( Chan Il Park ) 한국어병학회 2010 한국어병학회지 Vol.23 No.3

Bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP) are important components of the mammalian innate defence system against Gram-negative infections. The BPI/LBP cDNA was identified from the black rockfish ConA/PMA or LPS stimulated leukocyte cDNA library. The full-length BR-BPI/LBP cDNA was 2118 bp long and contained an open reading frame (ORF) of 1422 bp that encoded 473 amino-acid residues. The 5` UTR had a length of 57 bp, and the 3` UTR 639 bp. The molecular weight and theoretical isoelectric point (pI) values were calculated 51.4 kDa and 9.72, respectively. Compared with other known BPI or BPI/LBP peptide sequences, the most conserved regions of the black rockfish BPI/LBP peptide were found to be the BPI1 N-terminal, BPI2 C-terminal domains and a LPS binding domain. Phylogenetic analysis based on the deduced amino acid sequence revealed a homologous relationship between the BPI/LBP sequence of black rockfish and that of other teleosts. The black rockfish BPI/LBP gene was predominantly expressed in the PBLs, head kidney, trunk kidney and spleen. The expression of the black rockfish BPI/LBP molecule was induced in the peripheral blood leukocytes (PBLs) from 1 to 24 h following LPS stimulation, with a peak at 12 h post-stimulation.

Molecular cloning and expression analysis of a C-type lectin in the rock bream, Oplegnathus fasciatus

Kwon, Mun-Gyeong,Kim, Ju-Won,Park, Myoung-Ae,Hwang, Jee-Youn,Park, Hyung-Jun,Park, Chan-Il The Korean Society of Fish Pathology 2012 한국어병학회지 Vol.25 No.1

C-type lectins are crucial for pathogen recognition, innate immunity, and cell-cell interactions. In this study, a C-type lectin gene was cloned from the rock bream. The full-length RbCTL cDNA was 729 bp with a 429 bp ORF encoding a 164-residue protein. The deduced amino acid sequence of RbCTL had all of the conserved features crucial for its fundamental structure, including the four cysteine residues involved in sulfide bridge formation and potential $Ca^2+$/carbohydrate-binding sites. RbCTL contains a signal peptide one single carbohydrate recognition domain. It showed 29.4% similarity to the C-type lectin of rainbow trout. RbCTL mRNA was predominately expressed in gill and head-kidney tissue and expressed less in peripheral blood leukocytes, trunk-kidney, spleen, liver, intestine and muscle. Expression of RbCTL was differentially upregulated in rock bream stimulated with LPS, Con A/PMA and poly I:C.

The immune-adjuvant effect and safety of recombinant CC chemokine 1 (rRbCC1) in rock bream, Oplegnathus fasciatus

Kwon, Mun-Gyeong,Kim, Ju-Won,Hwang, Seong-Don,Kim, Eun-Gyeong,Park, Dae-Won,Park, Chan-Il The Korean Society of Fish Pathology 2013 한국어병학회지 Vol.26 No.3

Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.

EFFECTS OF IRON-LIMITATION ON THE PATHOGENICITY AND IMMUNOGENICITY OF Photobacterium damselae subsp. damselae

Mun Gyeong Kwon,Myoung Ae Park,Soo-Il Park 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

The immune-adjuvant effect and safety of recombinant CC chemokine 1 (rRbCC1) in rock bream, Oplegnathus fasciatus

( Mun Gyeong Kwon ),( Ju Won Kim ),( Seong Don Hwang ),( Eun Gyeong Kim ),( Dae Won Park ),( Chan Il Park ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3

Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.