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- 저자 : ( Muhammad Aamer Mehmood ) (검색결과 5 건)
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Wang Ning,Li Linman,Zhang Puyu,Mehmood Muhammad Aamer,Lan Chaohua,Gan Tian,Li Zaixin,Zhang Zhi,Xu Kewei,Mo Shan,Xia Gang,Wu Tao,Zhu Hui 한국영양학회 2023 Nutrition Research and Practice Vol.17 No.4
BACKGROUND/OBJECTIVES: Tibetan tea is a kind of dark tea, due to the inherent complexity of natural products, the chemical composition and beneficial effects of Tibetan tea are not fully understood. The objective of this study was to unravel the composition of Tibetan tea using knowledge-guided multilayer network (KGMN) techniques and explore its potential antioxidant and hypolipidemic mechanisms in mice. MATERIALS/METHODS: The C57BL/6J mice were continuously gavaged with Tibetan tea extract (T group), green tea extract (G group) and ddH2O (H group) for 15 days. The activity of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in mice was detected. Transcriptome sequencing technology was used to investigate the molecular mechanisms underlying the antioxidant and lipid-lowering effects of Tibetan tea in mice. Furthermore, the expression levels of liver antioxidant and lipid metabolism related genes in various groups were detected by the real-time quantitative polymerase chain reaction (qPCR) method. RESULTS: The results showed that a total of 42 flavonoids are provisionally annotated in Tibetan tea using KGMN strategies. Tibetan tea significantly reduced body weight gain and increased T-AOC and SOD activities in mice compared with the H group. Based on the results of transcriptome and qPCR, it was confirmed that Tibetan tea could play a key role in antioxidant and lipid lowering by regulating oxidative stress and lipid metabolism related pathways such as insulin resistance, P53 signaling pathway, insulin signaling pathway, fatty acid elongation and fatty acid metabolism. CONCLUSIONS: This study was the first to use computational tools to deeply explore the composition of Tibetan tea and revealed its potential antioxidant and hypolipidemic mechanisms, and it provides new insights into the composition and bioactivity of Tibetan tea.
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Crop Residues Management for Rice-Wheat Cropping System in Saline-Sodic Soil
Ahmed, Khalil,Qadir, Ghulam,Jami, Abdul-Rehman,Rafa, Hafeezullah,Mehmood, Muhammad Aamer,Han, Kyung-Hwa,Ibrahim, Muhammad Korean Society of Soil Science and Fertilizer 2014 한국토양비료학회지 Vol.47 No.4
Series of field experiments were conducted to evaluate the long term effect of gypsum and crop residue on crop yield and soil health in rice-wheat crop rotation system in salt affected soil. A saline-sodic field having $EC_e$ (electrical conductivity of the saturation extract) 4.77 ($dSm^{-1}$); pH ($H_2O$) 8.96; SAR 43.78 ($mmol\;L^{-1}$) and gypsum requirement (G.R.) 2.86 (Mg $acre^{-1}$) was selected on Soil Salinity Research Institute Farm. Five treatments consisting of ($T_1$) control, ($T_2$) gypsum at 100% G.R., ($T_3$) gypsum at 25% G.R. + wheat straw at $3Mg\;ha^{-1}$, ($T_4$) gypsum at 25% G.R. + rice straw at $3Mg\;ha^{-1}$, ($T_5$) gypsum at 25% G.R.+ rice and wheat straw at $3Mg\;ha^{-1}$ were replicated four times under completely randomized block design. The data indicated that grain and straw yield of rice and wheat was significantly (P<0.05) increased by all the amendments used either single or in combination. $T_2$ (gypsum at 100% G.R.) significantly (P<0.05) increased grain and straw yield of rice and wheat crops followed by $T_3$ (gypsum at 25% G.R. + wheat straw at $3Mg\;ha^{-1}$) when compared with control. Soil properties were also improved by used amendments, pronounced decreased in $EC_e$, $pH_s$ and SAR were recorded in $T_2$ followed by $T_3$. The efficiency of the treatments could be arranged in following order gypsum at 100% G.R.> gypsum at 25% G.R. + wheat straw at $3Mg\;ha^{-1}$ > gypsum at 25% G.R. + rice and wheat straw at $3Mg\;ha^{-1}$ > gypsum at 25% G.R. + rice straw at $3Mg\;ha^{-1}$ > control.
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Crop Residues Management for Rice-Wheat Cropping System in Saline-Sodic Soil
Khalil Ahmed,Ghulam Qadir,Abdul-Rehman Jami,Hafeezullah Rafa,Muhammad Aamer Mehmood,Kyung-Hwa Han,Muhammad Ibrahim 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.4
Series of field experiments were conducted to evaluate the long term effect of gypsum and crop residue on crop yield and soil health in rice-wheat crop rotation system in salt affected soil. A saline-sodic field having ECe (electrical conductivity of the saturation extract) 4.77 (dS m<SUP>-1</SUP>); pH (H₂O) 8.96; SAR 43.78 (mmol L<SUP>-1</SUP>) and gypsum requirement (G.R.) 2.86 (Mg acre<SUP>-1</SUP>) was selected on Soil Salinity Research Institute Farm. Five treatments consisting of (T₁) control, (T₂) gypsum at 100% G.R., (T₃) gypsum at 25% G.R. + wheat straw at 3 Mg ha<SUP>-1</SUP>, (T₄) gypsum at 25% G.R. + rice straw at 3 Mg ha<SUP>-1</SUP>, (T?) gypsum at 25% G.R.+ rice and wheat straw at 3 Mg ha<SUP>-1</SUP> were replicated four times under completely randomized block design. The data indicated that grain and straw yield of rice and wheat was significantly (P<0.05) increased by all the amendments used either single or in combination. T₂ (gypsum at 100% G.R.) significantly (P<0.05) increased grain and straw yield of rice and wheat crops followed by T₃ (gypsum at 25% G.R. + wheat straw at 3 Mg ha<SUP>-1</SUP>) when compared with control. Soil properties were also improved by used amendments, pronounced decreased in ECe, pHs and SAR were recorded in T₂ followed by T₃. The efficiency of the treatments could be arranged in following order gypsum at 100% G.R.> gypsum at 25% G.R. + wheat straw at 3 Mg ha<SUP>-1</SUP>> gypsum at 25% G.R. + rice and wheat straw at 3 Mg ha<SUP>-1</SUP>> gypsum at 25% G.R. + rice straw at 3 Mg ha<SUP>-1</SUP>> control.
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( Xiang Xiao ),( Feng Ping Wang ),( Xu Ke ),( Xi Xiang Tang ),( Ying Bao Gai ),( Muhammad Aamer Mehmood ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.3
A psychrotrophic bacterium, Arthrobacter sp. ON14, isolated from Antarctica, was shown to exhibit a high β-galactosidase activity at a low temperature. A genomic library of ON14 was constructed and screened for β-galactosidase genes on functional plates containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) as the substrate. Two different β-galactosidase genes, named as galA, galB, were found in ON14. Computational analyses of the genes revealed that the encoded protein GalA belongs to family 2 of glycosyl hydrolysases and is a cold-active protein, whereas GalB belongs to family 42 of glycosyl hydrolysases and is a mesophilic protein. Reverse transcription analyses revealed that the expression of galA is highly induced at a low temperature (4℃) and repressed at a high temperature (28℃) when lactose is used as the sole carbon source. Conversely, the expression of galB is inhibited at a low temperature and induced at a high temperature. The purified GalA showed its peak activity at 15℃ and pH 8. The mineral ions Na+, K+, Mg2+, and Mn2+ were identified as enzyme activators, whereas Ca2+ had no influence on the enzyme activity. An enzyme stability assay revealed that the activity of GalA is significantly decreased when it is incubated at 45℃ for 2 h, and all its activity is lost when it is incubated at 50℃.
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