Erratum to “A Study of the Anti-inflammatory Effect of Protein Derived from Tenebrio molitor Larvae”

Minchul Seo(서민철),Hwa Jeong Lee(이화정),Joon Ha Lee(이준하),Minhee Baek(백민희),In-Woo Kim(김인우),Sun Young Kim(김선영),Jae-Sam Hwang(황재삼),Mi-Ae Kim(김미애) 한국생명과학회 2019 생명과학회지 Vol.29 No.10

Identification of novel cell migration‐promoting genes by a functional genetic screen

Seo, Minchul,Lee, Won‐,Ha,Suk, Kyoungho Federation of American Society for Experimental Bi 2010 The FASEB Journal Vol.24 No.2

Osteoblastogenic Activity of Tenebrio molitor Larvae Oil on the MG-63-Osteoblastic Cell

Minchul Seo,Minhee Baek,Joon Ha Lee,Hwa Jeong Lee,In-Woo Kim,Sun Young Kim,Jae-Sam Hwang,Mi-Ae Kim 한국식품영양과학회 2019 한국식품영양과학회 학술대회발표집 Vol.2019 No.10

Central Administration of Tenebrio molitor Larvae Extract Regulates Food Intake and Body Weight in Obesity Mice

Minchul Seo,Joon Ha Lee,Minhee Baek,Hwa Jeong Lee,Jae-Sam Hwang,Mi-Ae Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

We recently reported the in vitro and in vivo antiobesity effects of Tenebrio molitor larvae, a traditional food in manycountries, but it remains unknown how the larvae affect appetite regulation in mice with diet-induced obesity. We hypothesizedthat the extract of T. molitor larvae mediates appetite by regulating neuropeptide expression. We investigated T. molitorlarvae extract's (TME's) effects on anorexigenesis and endoplasmic reticulum (ER) stress–induced orexigenic neuropeptideexpression in the hypothalami of obese mice. Central administration of TME suppressed feeding by down-regulating theexpression of the orexigenic neuropeptides neuropeptide Y and agouti-related protein. T. molitor larvae extract significantlyreduced the expression of ER stress response genes. These results suggest that TME and its bioactive components arepotential therapeutics for obesity and ER stress–driven disease states.

A novel role for earthworm peptide Lumbricusin as a regulator of neuroinflammation

Seo, Minchul,Lee, Joon Ha,Baek, Minhee,Kim, Mi-Ae,Ahn, Mi-Young,Kim, Seong Hyun,Yun, Eun-Young,Hwang, Jae-Sam Elsevier 2017 Biochemical and biophysical research communication Vol.490 No.3

<P><B>Abstract</B></P> <P>Recently, we reported that Lumbricusin, an antimicrobial peptide isolated from earthworm <I>Lumbricus terrestris</I>, enhanced neuronal proliferation and ameliorated motor dysfunction and dopaminergic neurodegeneration. Accumulating evidence suggests that neurodegeneration is the primary pathological feature of acute or chronic inflammation mediated by microglia, the resident macrophage of the central nervous system. Therefore, microglial activation inhibitors may be useful as therapeutic agents for neurodegenerative diseases. To determine whether Lumbricusin ameliorates neuroinflammation through inhibition of microglial activation by lipopolysaccharides (LPS), we newly synthesized 9-mer Lumbricusin analogues based on the amino acid sequence of Lumbricusin. One of these, Lumbricusin Analogue 5 (LumA5; QLICWRRFR-NH<SUB>2</SUB>), markedly reduced expression of enzymes (COX-2, iNOS), cytokines (IL-6, IL-1β, TNF-α), and signal transduction factors (AKT, MAPKs, NF-κB) involved in inflammation triggered by LPS <I>in vitro</I> and <I>in vivo</I>. In addition, LumA5 inhibited the cytotoxicity of conditioned medium prepared by LPS-activated BV-2 microglia to neuronal SH-SY5Y cells and improved cell viability. These results indicate that LumA5 may be a potential therapeutic agent for the treatment of various neuroinflammatory conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lumbricusin, an antimicrobial peptide, showed anti-inflammatory properties. </LI> <LI> LumA5, an analogue of Lumbricusin, reduced cytokines in activated microglia. </LI> <LI> LumA5 reduced the proinflammatory phosphorylation of MAPKs and AKT. </LI> <LI> LumA5 attenuated neurotoxicity <I>in vitro</I> and neuroinflammation <I>in vivo.</I> </LI> </UL> </P>

<i>Tenebrio molitor</i> Larvae Inhibit Adipogenesis through AMPK and MAPKs Signaling in 3T3-L1 Adipocytes and Obesity in High-Fat Diet-Induced Obese Mice

Seo, Minchul,Goo, Tae-Won,Chung, Mi Yeon,Baek, Minhee,Hwang, Jae-Sam,Kim, Mi-Ae,Yun, Eun-Young MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.3

<P>Despite the increasing interest in insect-based bioactive products, the biological activities of these products are rarely studied adequately. Larvae of <I>Tenebrio molitor</I>, the yellow mealworm, have been eaten as a traditional food and provide many health benefits. Therefore, we hypothesized that <I>T. molitor</I> larvae might influence adipogenesis and obesity-related disorders. In the present study, we investigated the anti-adipogenic and antiobesity effects of <I>T. molitor</I> larvae in vitro and in vivo. The lipid accumulation and triglyceride content in mature adipocytes was reduced significantly (up to 90%) upon exposure to an ethanol extract of <I>T. molitor</I> larvae, without a reduction in cell viability. Exposure also resulted in key adipogenic and lipogenic transcription factors. Additionally, in adipogenic differentiation medium the extract induced phosphorylation of adenosine monophosphate (AMP)-activated protein kinase and mitogen-activated protein kinases. Daily oral administration of <I>T. molitor</I> larvae powder to obese mice fed high-fat diet attenuated body weight gain. We also found that the powder efficiently reduced hepatic steatosis as well as aspartate and alanine transaminase enzyme levels in mice fed a high-fat diet. Our results suggest that <I>T. molitor</I> larvae extract has an antiobesity effect when administered as a food supplement and has potential as a therapeutic agent for obesity.</P>

Role of the p55-γ subunit of PI3K in ALK-induced cell migration: RNAi-based selection of cell migration regulators

Seo, Minchul,Kim, Jong-Heon,Suk, Kyoungho LANDES BIOSCIENCE 2017 Cell adhesion & migration Vol.11 No.3

Antioxidant Activity and Hair Growth Promoting Effect of Mealworm (Tenebrio molitor larvae) Extract

Minchul Seo,Mi-Ae Kim,Eun-Young Yun,Jae-Sam Hwang,Minhee Baek 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

The insects have been investigated as novel sources for foods and biomaterials in several recent studies. However,its effects on hair growth have not been sufficiently researched. To develop novel and natural materials for preventingalopecia and promoting hair growth, we investigated the antioxidant activity and hair growth promoting effect of Tenebriomolitor larvae extract (TME). As a result, DPPH radical scavenging activity was 81.17%, and nitrite scavenging activitywas 43.69%, which were similar to blueberry extract. And TME promoted the proliferation of human DPCs and NIH3T3cells, concentration dependently. In addition, the TME prevented not only DHT-induced DPCs cytotoxicity but also actionof TBM as a potassium channel blocker in NIH3T3 cells. These results suggest that TME can be used as a functionalmaterial for alopecia therapeutic reagent by preventing hair loss and promoting hair growth.

BV-2 미세아교세포에서 왕귀뚜라미 유래 Teleogryllusine의 신경염증 억제 효과

서민철(Minchul Seo),신용표(Yong Pyo Shin),이화정(Hwa Jeong Lee),백민희(Minhee Baek),이준하(Joon Ha Lee),김인우(In-Woo Kim),황재삼(Jae-Sam Hwang),김미애(Mi-Ae Kim) 한국생명과학회 2020 생명과학회지 Vol.30 No.11

최근 중추신경계에서 면역기능을 담당하는 미세아교세포(microglia)의 염증반응을 효율적으로 조절하는 것은 알츠하이머 병, 파킨슨 병 및 헌팅턴 병과 같은 퇴행성 뇌질환의 치료를 위한 중요한 타겟으로 인식되고 있다. 왕귀뚜라미(Teleogryllus emma)는 다양한 치료효능으로 인해 세계적으로 널리 이용되고 있으며, 본 연구팀에서는 최근 왕귀뚜라미의 전사체 분석을 통하여 항균활성을 가지는 다양한 종류의 새로운 항균 펩타이드(antimicrobial peptide; AMP) 후보들을 선별한 바 있다. 항균 펩타이드는 미생물에서부터 포유류까지 매우 다양한 종으로부터 발견되었으며, 현재는 항균활성뿐만 아니라 염증반응과 같은 다양한 질병의 치료제 개발을 위한 후보 물질로 관심을 받고 있다. 본 연구에서는 선행연구를 통하여 선별된 왕귀뚜라미 유래 항균 펩타이드들 중에서 Teleogryllusine (VKWKR-LNNNKVLQKIYFVKI-NH₂)으로 명명된 항균 펩타이드의 신경염증 억제 효능을 관찰하였다. Teleogryllusine의 신경염증 억제 효능을 관찰 하기 위하여 immortalized mouse microglia 세포주인 BV-2 세포에 Teleogryllusine을 1시간 전처리 한 후 LPS를 이용하여 BV-2 세포의 염증 반응을 유도하였다. 그 결과 Teleogryllusin은 최대 처리 농도인 80 μg/ml까지 세포독성 없이 nitric oxide (NO) 생성을 현저히 감소시킴을 확인할 수 있었다. 또한 염증반응 매개인자인 iNOS와 COX-2 및 cytokine (Il-6, TNF-α)의 발현을 유전자 수준과 단백질 수준에서 확인한 결과 Teleogryllusine 처리농도에 의존적으로 감소됨을 확인할 수 있었다. 또한 Teleogryllusine의 신경염증 억제작용 기작을 확인한 결과 mitogen activated protein kinases (MAPKs)와 IκB의 인산화 및 proteosome에 의한 IκB의 분해를 억제함으로서 BV-2 세포의 신경염증반응이 조절됨을 확인할 수 있었다. 이러한 결과로 보아 왕귀뚜라미 유래 Teleogryllusine 펩타이드는 신경염증반응에 의해 유도되는 퇴행성 뇌질환 치료 및 예방을 위한 의료용 소재로 사용될 수 있을 것으로 기대된다. The suppression of neuroinflammatory responses in microglial cells, well known as the main immune cells in the central nervous system (CNS), are considered a key target for improving the progression of neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease. Teleogryllus emma is widely consumed around the world for its broad-spectrum therapeutic effect. In a previous work, we performed transcriptome analysis on T. emma in order to obtain the diversity and activity of its antimicrobial peptides (AMPs). AMPs are found in a variety of species, from microorganisms to mammals. They have received much attention as candidates oftherapeutic drugs for the treatment of inflammation-associated diseases. In this study, we investigated the anti- neuroinflammatory effect of Teleogryllusine (VKWKRLNNNKVLQKIYFVKI-NH₂) derived from T. emma on lipopolysaccharide (LPS) induced BV-2 microglia cells. Teleogryllusine significantly inhibited nitric oxide (NO) production without cytotoxicity, and reducing pro-inflammatory enzymes expression such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, Telegryllusine also inhibited the expression of pro-inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) through down-regulation of the mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) signaling pathway. These results suggest that T. emma- derived Teleogryllusine could be a good source of functional substances that prevent neuroinflammation and neurodegenerative diseases.

갈색거저리 유충 오일이 MG-63 조골세포 분화에 미치는 영향

서민철(Minchul Seo),백민희(Minhee Baek),이준하(Joon Ha Lee),이화정(Hwa Jeong Lee),김인우(In-Woo Kim),김선영(Sun Young Kim),황재삼(Jae-Sam Hwang),김미애(Mi-Ae Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.9

현재 우리나라는 고령화 사회에 접어들었으며, 이와 함께 골 대사 질환이 큰 문제로 대두되고 있다. 그 중 가장 흔하게 발생하는 골다공증은 여러 요인에 의해 발생하고 있으며 이의 예방 및 치료를 위한 소재 개발 연구가 활발하게 진행되고 있다. 그러나 곤충을 소재로 한 골다공증 예방 및 치료 연구는 미흡한 실정이다. 따라서 본 연구에서는 골 형성 촉진 효능을 가진 새로운 소재 개발을 위해 갈색거저리 유충 오일의 MG-63 조골세포의 분화 촉진효과를 연구하였다. 조골세포에서 갈색거저리 유충 오일의 독성 및 증식 효과를 평가하기 위하여 MTS assay를 진행한 결과 80 μg/ml 농도까지 세포 독성이 나타나지 않았으며, 48시간 배양했을 때 40-80 μg/ml 농도에서 120% 정도의 세포 증식 효능을 확인 하였다. 갈색거저리 유충 오일이 조골세포 분화에 미치는 영향을 확인하기 위하여 5일간 갈색거저리 유충 오일을 MG-63 조골세포에 처리한 후 ALP 활성을 측정하였다. 그 결과 80 μg/ml 농도에서 무처리 군에 비해 약 180%의 조골세포 분화 촉진 효능을 관찰 하였다. 이 결과는 ALP staining에서도 유사하게 나타났다. mRNA 발현량의 변화를 측정한 결과, Alpl과 Runx2 유전자 발현량이 증가하였고, 단백질 발현량을 측정했을 때에도 유사한 결과를 확인하였다. 이를 통해 ALP와 Runx2 유전자 및 단백질 발현에 의해서 ALP 활성이 증가하고 조골세포 분화가 촉진되었을 것으로 판단되며, 갈색거저리 유충 오일을 이용한 골 형성촉진에 따른 골다공증 예방 및 치료 기능성 소재 개발에 대한 가능성을 확인하였다. Recently, Korea has seen a rapid increase in the elderly population. As a result, osteoporosis, a geriatric disease, has become a social problem. To investigate the novel and natural materials for promoting osteoblastogenesis, we investigated the osteoblastogenic activity of Tenebrio molitor larvae oil (TMO) on the MG-63 preosteoblast cells. The cytotoxicity and proliferation effects of TMO on MG-63 cells were measured by MTS assay. There was no cytotoxicity up to 80 μg/ml. At 40 and 80 μg/ml of TMO (treated for 48 hr), cell proliferation was elevated about 120% compared to the control. The osteoblastogenic activity of TMO was measured with alkaline phosphatase (ALP) activity at 5 days. Doses of 5 to 80 μg/ml of TMO increased ALP activities significantly compared with the control. In addition, expression of ALP and Runx2 (osteoblastogenic markers) were markedly increased after treatment of TMO for 5 days. These results provide evidence that TMO promotes osteoblastogenesis by increasing the gene and protein expression of ALP and Runx2, and they suggest that TMO may be a potential agent for bone formation and preventing osteoporosis.