http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
Seo, Min-Seock,Hwang, Kyung-Gyun,Kim, Hyong-Bum,Baek, Seung-Ho The Korean Academy of Conservative Dentistry 2012 Restorative Dentistry & Endodontics Vol.37 No.3
Objectives: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization. Materials and Methods: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data. Results: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated. Conclusions: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.
Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
Min-Seock Seo,Kyung-Gyun Hwang,Hyongbum Kim,Seung-Ho Baek 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.3
Objectives: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization. Materials and Methods: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RTPCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data. Results: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated. Conclusions: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast. (Restor Dent Endod 2012;37(3):142-148)
Pectinase-treated <i>Panax ginseng</i> protects heat stress-induced testicular damage in rats
Kim, Min Kyoung,Cha, Kyu-Min,Hwang, Seock-Yeon,Park, Un-Kyu,Seo, Seok Kyo,Lee, Sang-Ho,Jeong, Min-Sik,Cho, SiHyun,Kopalli, Spandana Rajendra,Kim, Si-Kwan BioScientifica Ltd 2017 Reproduction Vol.153 No.6
<P>Testicular hyperthermia is well studied to cause impaired spermatogenesis. In the present study, the protective effect of enzymatically modified (pectinase-treated) Panax ginseng (GINST) against intermittent sub-chronic heat stress-induced testicular damage in rats was investigated. Male Sprague-Dawley rats were divided into four groups: normal control (NC), heat-stressed control (HC), heat- stressed plus GINST-100mg/kg/day (HG100) and heat- stressed plus GINST-200mg/kg/day (HG200) treatment groups. GINST (100 and 200mg/kg/day) was mixed separately with a regular pellet diet and was administered orally for 8 weeks starting from 1 week before heat exposure. Parameters such as organ weight, blood chemistry, sperm kinetic values, expression of antioxidant enzymes, spermatogenesis molecules and sex hormone receptors levels were measured. Data revealed that kidney and epididymis weight were significantly (P < 0.05) decreased with heat stress and recovered by GINST treatment. Further, the altered levels of blood chemistry panels and sperm kinetic values in heat stress-induced rats were attenuated when GINST was administered (P < 0.05). Furthermore, the expression levels of antioxidant-related enzymes (GSTM5 and GPX4), spermatogenesis-related proteins (CREB1 and INHA) and sex hormone receptors (androgen receptor, luteinizing hormone receptor and follicle-stimulating hormone receptor) were reduced by heat stress; however, GINST treatment effectively ameliorated these changes. In conclusion, GINST was effective in reducing heat-induced damage in various male fertility factors in vivo and has considerable potential to be developed as a useful supplement in improving male fertility.</P>
Seoul Environmental health policy - people and their environment, human health and well-being
Seung-Mi KWON,Cheol-Min LEE,Kwang-Rae KIM,Chang-Mo KIM,Soo-Seock CHO,Min-Jeong SEO,Mi-Hee JANG,Jin-Sol PARK,Ho-Jun Rhee,Eun-Sun PARK,Byung-Chul MIN,Sang-Hoon LEE,Myung-Kyu PARK,Min-Chul KIM,Jin-Ho SHI 한국유통과학회 2023 International Forum on Business Convergence (IFBC) Vol.2023 No.-
Cytokinin Stimulates Expression of the Chloroplast ATP Synthase VI Subunit Gene(atpl)
Lee, Eung Gwan,Seo, Jeong Seock,Cha, Min Ho,Suh, Mi Chung,Sim, Woong Seop 한국식물학회 2002 Journal of Plant Biology Vol.45 No.2
To investigate the signal transduction pathway of cytokinin, we incubated seven-day-old maize leaves in distilled water with or without 0.1mM benzylaminopurine (BAP). Several gene fragments were either induced or repressed by this exogenous treatment, and were then isolated by differential display-polymerase chain reaction (DD-PCR). One fragment (IBC4) had significant sequence homology with the N-terminal portion of the chloroplast ATP synthase Ⅳ subunit gene (atpl) of higher plants. After the stimulated expression of IBC4 by BAP was confirmed using orthern blot hybridization, we isolated the full-length gene, including IBC4, from the chloroplast genome. We verified it as an atpl gene, using BLAST search analysis. This atpl gene, designated as Zm-atpl (Zea mays-atpl), is 744 nucleotides long and encodes 247 amino acids. Its deduced amino acid sequence does not vary among maize cultivars, and it shares >90% identity with the atpl genes from Oryza sativa, Triticum aestivum, Nicotiana tabacum, Spinacia oleracea, and Pisum sativum. We also confirmed, by northern blot analysis, that expression of Zm-atpl transcripts was significantly stimulated by the exogenous application of BAP.
Lee, Hee Jin,Seo, An Na,Kim, Eun Joo,Jang, Min Hye,Suh, Koung Jin,Ryu, Han Suk,Kim, Yu Jung,Kim, Jee Hyun,Im, Seock-Ah,Gong, Gyungyub,Jung, Kyung Hae,Park, In Ae,Park, So Yeon American Society for Clinical Pathology 2014 American journal of clinical pathology Vol.142 No.6
<P><B>Objectives:</B></P><P>Heterogeneity of <I>HER2</I> gene amplification is found in a subset of breast cancers. We investigated the impact of <I>HER2</I> heterogeneity on trastuzumab responses and clinical outcomes in 112 patients with HER2-positive metastatic breast cancer.</P><P><B>Methods:</B></P><P>Regional and genetic heterogeneity of <I>HER2</I> gene amplification was determined in three different areas of each tumor by immunohistochemistry and silver in situ hybridization. We also assessed the overall levels of <I>HER2</I> amplification and the proportion of tumor cells with a <I>HER2</I>/CEP17 ratio of more than 2.2 or strong and complete membranous (3+) expression of HER2 protein.</P><P><B>Results:</B></P><P><I>HER2</I> regional and genetic heterogeneity based on the <I>HER2</I>/CEP17 ratio was confirmed in 8.7% and 2.7% of cases, respectively. Poor response to trastuzumab was associated with overall low-level or equivocal amplification, <I>HER2</I> regional heterogeneity by the <I>HER2</I>/CEP17 ratio, the <I>HER2</I>/CEP17 ratio of more than 2.2 in less than 80% of tumor cells, and <I>HER2</I> immunohistochemical expression of 3+ in less than 75% of tumor cells. In survival analyses, low-level or equivocal <I>HER2</I> amplification, <I>HER2</I> regional heterogeneity based on the <I>HER2</I>/CEP17 ratio, and the <I>HER2</I>/CEP17 ratio of more than 2.2 in less than 80% of tumor cells were associated with shorter time to progression and lower overall survival in univariate and multivariate analyses.</P><P><B>Conclusions:</B></P><P>These results suggest that accurate assessment of <I>HER2</I> status, including <I>HER2</I> heterogeneity, is important in predicting trastuzumab responses and outcomes in patients with HER2-positive metastatic breast cancer.</P>