과열증기처리 반탄화 추출물의 항산화 효과 검증에 관한 연구

오근혜(Geun Hye Oh),남정빈(Jeong Bin Nam),양승민(Seung Min Yang),정원희(Won Hee Joung),정진산(Jin San Jeong),신종민(Jong Min Shin),강석구(Seog Goo Kang) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.2

The antioxidant properties of the extracts from torrefied wood subjected to superheated steam treatment were investigated. Total polyphenolics, total flavonoids, DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity, reducing power, and FRAP (Ferric reducing antioxidant power) were determined to evaluate antioxidant activity. The total polyphenol content was 879.67±40.41 ㎎/mL at a concentration of 25 g/mL when the extract was subjected to a temperature of 300℃ for 10 min, and 759.67±25.17 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value of at 300℃ was 15.79% higher than that obtained at 350℃. Total flavonoid content was 111.18±3.55 ㎎/mL at a concentration of 25 g/mL at 300℃ for 10 min, and 80.58±2.58 ㎎/mL at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 37.97% higher than that obtained at 350℃. For the DPPH free radical scavenging activity, the highest scavenging activity was observed at a concentration of 6 g/mL. DPPH free radical scavenging activity at a concentration of 6 g/mL at 300℃ for 10 min was 89.83±0.03%, whereas at 350℃ for 10 min at the same concentration, it was found to be 87.99±0.1%. The value at 300℃ was 2.09% higher than that obtained at 350℃. Reducing power was determined to be 3.59±0.04 at a concentration of 25 g/mL at 300℃ for 10 min, and 2.92±0.1 at a concentration of 25 g/mL at 350℃ for 10 min. The value at 300℃ was 22.94% higher than that obtained at 350℃. FRAP was 1742±37 μM at a concentration of 25 g/mL at 300℃ for 10 min, and 1106±8 μM at a at 350℃ for 10 min. At 300℃, the FRAP value was 57.50% higher than that obtained at 350℃. Based on these results, we suggest that torrefied wood treated with superheated steam can be used in various applications because of its effective antioxidant properties.

Highly mesoporous activated carbon fibers using high density polyethylene precursor with a multi-step stabilization technique for automobile carbon canister

Jeong-Rae Ahn,Hye-Min Lee,Byung-Joo Kim 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

In this study, activated carbon fibers (ACFs) for automobile carbon canisters were produced usinghigh-density polyethylene (HDPE) fibers treated with a multistep stabilization process as the precursor. The stabilization of the HDPE fibers involved the following sequential steps: (1) electron beamirradiation, (2) mild sulfonation, and (3) dry oxidation. The stabilized fibers were carbonized at 900℃in a nitrogen atmosphere. The carbonized fibers were activated at 900℃ in a water–vapor atmospherefrom 30-60 min. The textural properties of the activated carbon fibers were determined using N2/77 Kadsorption–desorption isotherms. The specific surface area ranged from 1090 to 1980 m2/g, and the totalpore volume ranged from 0.56 to 1.11 cm3/g. The specific surface area, micropore volume, and mesoporevolume increased with increasing activation time. The fractions of micropores and mesopores were similaracross all conditions, whereas the yield decreased gradually until 50 min, and then exhibited a sharpdecline at 60 min. The butane working capacity increased as much as 56.24% as the increase in activationtime. This can be attributed to the increase in butane activity owing to the increase in micropore volume,along with a decrease in butane resistivity caused by the increase in mesopore volume at a specificdiameter.

Dose-linear pharmacokinetics of oleanolic acid after intravenous and oral administration in rats

Jeong, Dong Won,Kim, Young Hoon,Kim, Hui Hyun,Ji, Hye Young,Yoo, Sun Dong,Choi, Won Rack,Lee, Soo Min,Han, Chang-Kyun,Lee, Hye Suk WILEY PUBLISHERS 2007 BIOPHARMACEUTICS AND DRUG DISPOSITION Vol.28 No.2

<P>The pharmacokinetics of oleanolic acid was evaluated in vitro and in vivo. From Caco-2 cell permeation studies, oleanolic acid was a low permeability compound with no directional effects, suggesting a low in vivo absorption mediated by a passive diffusion. Oleanolic acid was metabolically unstable following incubation with rat liver microsomes in the presence of NADPH. After intravenous injection at doses of 0.5, 1 and 2 mg/kg doses, oleanolic acid showed dose-linear pharmacokinetics as evidenced by unaltered CL (28.6–33.0 ml/min/kg), V<SUB>ss</SUB> (437–583 ml/kg), dose-normalized AUC (16.0–17.9 µg min/ml based on 1 mg/kg) and t<SUB>1/2</SUB> (41.9–52.7 min). Following oral administration of oleanolic acid at doses of 10, 25 and 50 mg/kg, T<SUB>max</SUB>, t<SUB>1/2</SUB>, dose-normalized C<SUB>max</SUB> (66–74 ng/ml based on 25 mg/kg) and dose-normalized AUC (5.4–5.9 µg min/ml based on 25 mg/kg) were comparable between 25 and 50 mg/kg dose, but the plasma concentrations at 10 mg/kg dose were not measurable as they were below the limit of quantitation (2 ng/ml). The absolute oral bioavailability was 0.7% for oral doses of 25 and 50 mg/kg. The extent of urinary excretion was minimal for both i.v. and oral doses. The very low oral bioavailability of oleanolic acid could be due to a poor absorption and extensive metabolic clearance. Copyright © 2007 John Wiley & Sons, Ltd.</P>

인체혈장 중 에탐부톨의 HPLC 분석법의 검증 및 단일용량 투여에 의한 약물동태 연구

곽혜선,박경호,최준식,송진아,성민경,장정옥,이화정 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

An HPLC method was employed for the determination of ethambutol in human plasma. After addition of internal standard (IS, octylamine, 2 μg/mL) and alkalinization of the plasma with 5 M sodium hydroxide, the drug and IS were extracted into the mixture of chloroform and diethyl ether (40:60, v/v). Following a 15-min vortex-mixing and a 10-min centrifugation, the organic phase was spiked with 100 pL of phenylethylisocyanate (2000 μg/mL) for chemical derivatization, mixed for 5 min and evaporated to dryness under a stream of nitrogen. The residue was reconstituted with 100 μL of mobile phase and 20 pL was injected into Cl8 column with a mobile phase consisting of methanol:water (70:30, v/v). The samples were detected utilizing an ultraviolet detector at 200 nm. The method was specific and validated with a limit of 0.15 μg/mL. Infra- and inter-day precision and accuracy were acceptable for all quality control samples including the lower limit of quantification. The applicability of this method was demonstrated by analysis of human plasma after oral administration of a single 1200-mg dose to 20 healthy subjects. From the plasma ethambutol concentration vs. time curves, the mean AUC was 9.61 ± 1.64 μg hr/mL. and Cmax of 2.68 μg/mL reached 2.73 hr after administration. The mean biological half-life of ethambutol was 3.46 ± 1.21 hr. Based on the results, this simple and validated assay could readily be used in any pharmacokinetic studies using humans.

반응표면분석에 의한 노랑느타리버섯 추출물의 추출조건 최적화

이혜진(Hye-Jin Lee),도정룡(Jeong-Ryong Do),정민유(Min-Yu Chung),김현구(Hyun-Ku Kim) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.10

노랑느타리버섯의 항산화 활성을 측정하여 효율적 추출조건을 예측하기 위해 마이크로웨이브 에너지, 에탄올 농도, 추출시간의 요인변수를 중심합성법에 따라 설정하여 반응표면분석을 통해 최적화 조건을 설정하였다. 노랑느타리버섯 추출물의 수율, 전자공여작용, SOD 유사 활성, 총 폴리페놀 함량에 대한 반응표면의 회귀식 R² 값이 각각 0.91, 0.87, 0.86 및 0.87로 분석되었고, 수율은 P<0.01, 항산화 활성이 P<0.05에서 유의적 수준을 나타냈다. 노랑느타리버섯은 요인변수에 따라 수율, 전자공여작용, 총 폴리페놀 함량의 경우 마이크로웨이브 에너지의 영향을 가장 많이 받았고, SOD유사 활성의 경우 에탄올 농도에 따른 유의적 차이를 보였다. 추출물의 각 특성을 모두 만족시키는 최적의 추출조건의 범위는 superimposing 하여 마이크로웨이브 에너지 71.48~92.84 watts, 에탄올 농도 55.01~71.66%, 추출시간 3~9분으로 예측되었다. Response surface methodology (RSM) was used to optimize extraction conditions of Pleurotus cornocopiae. Coefficients of determination (R²) for dependent variables ranged from 0.86 at 0.91. Maximum extraction yield was 40.81% under the following conditions: microwave power of 60.08 watts, ethanolic concentration of 12.33%, and extraction time of 6.86 min. Maximum extraction electron donating ability was 35.72% at 44.13 watts, 19.30%, and 4.21 min. Maximum extraction superoxide dismutase (SOD)-like activity was 34.87% at 114.01 watts, 65.88%, and 1.56 min. Maximum extraction total polyphenol content was 31.77 mg/g at 50.52 watts, 23.00% and 2.90 min. Based on the superimposition of four dimensional RSM with respect to extraction yield, electron donating ability, SOD-like activity, and total polyphenol content obtained under various extraction conditions, the optimum ranges of extraction conditions were as follows: microwave power of 71.48~92.84 watts, ethanol concentration of 55.01~71.66%, and extraction time of 3~9 min.

Determination of Authentic Chiisanoside in Acanthopanax senticousus by High Performance Liquid Chromatography

Lee, Jeong-Min,Kim, Min-Hye,Lee, Sang-Hyun The Plant Resources Society of Korea 2007 한국자원식물학회지 Vol.20 No.3

High performance liquid chromatography (HPLC) was used for the analysis of chiisanoside in each stem and root of Acanthopanax senticosus collected from South Korea, North Korea, China and Russia. A reverse-phase system using a gradient of H$_{2}$O and acetonitrile as the mobile phase was developed and detection was at 210nm. The analysis was successfully carried out within 30 min. Chiisanoside was measured in the stem and root of A. senticosus collected from various countries.

중소병원 간호사의 재직의도 영향요인 연구

김미혜(Mi-Hye Kim),박정민(Jeong-Min Park) 한국엔터테인먼트산업학회 2020 한국엔터테인먼트산업학회논문지 Vol.14 No.2

본 연구는 중소병원 간호사의 소명의식, 자기효능감 및 직장 내 괴롭힘이 재직의도에 미치는 영향요인을 살펴봄으로써, 간호조직의 업무환경 개선을 위한 기초자료를 제공하기 위해 시도된 서술적 조사연구이다. 자료 수집은 2018년 7월 17일에서 30일까지로 G광역시에 위치한 7개의 중소병원에서 6개월 이상 근무한 간호사 224명을 대상으로 하였으며, 수집된 자료는 SPSS / WIN 22.0 프로그램을 사용하여 분석하였다. 측정도구로 소명의식은 Dik, Eldrige와 Steger (2008)가 개발한 소명척도(Calling & Vocational Questionnaire: CVQ)를 윤치호(2017)가 수정한 도구를, 자기효능감은 Sherer 등(1982)이 개발하고 정애순(2007)이 수정하여 간호사를 대상으로 한 사용한 도구를 사용하였다. 직장 내 괴롭힘은 Einarsen, Raknes와 Matthiesen (1994)이 노르웨이어로 개발한 부정적 행동경험 설문지(NAQ)를 남웅 등(2010)이 한국어로 번안한 도구를, 재직의도는 Cowin (2002)이 개발한 NRI (Nurses’Retention Index)를 김민정(2006)이 번안하여 사용한 도구를 사용하였다. 재직의도에 영향을 미치는 요인을 분석한 결과 자기효능감(β=.24, p<.001), 소명의식(β=.23, p<.001), 연봉(β=.21, p=.001)은 통계적으로 유의한 양의 상관관계를, 직장 내 괴롭힘(β=-.16, p=.008)은 통계적으로 유의한 음의 상관관계를 미치는 것으로 나타났으며, 설명력은 28.6%이었습니다. 본 연구결과를 종합하면 중소병원에 근무하는 간호사의 재직의도에 긍정적 영향 요인은 소명의식, 자기효능감 및 연봉 수준이었고, 부정적인 영향 요인은 직장 내 괴롭힘 임을 확인하였다. Purpose: This study was a descriptive study to investigate on the effect of calling, self-efficacy, workplace bullying on retention intention among nurses in small and medium-sized hospitals. Methods: The research subjects were 224 nurses selected through convenient sampling from among those who have worked six months or longer in seven small and medium-sized hospitals located in G City. The data were collected using a structured questionnaire from July 17 to 30, 2018. The data was analyzed by descriptive statics, a t-test, ANOVA, Scheffé test, Pearson’s correlation coefficient, and a stepwise multiple regression using SPSS/WIN 22.0 program. As a measuring tool, Calling was the Calling & Vocational Questionnaire (CVQ) developed by Dik, Eldrige and Steger (2008). Self-efficacy was developed by Sherer et al. (1982) and modified by Jeong Ae-soon (2007). Workplace bullying was developed by Einarsen, Raknes and Matthiesen (1994) in the Norwegian Negative Behavioral Experience Questionnaire (NAQ), and by Nam Woong et al. (2010). The retention intention was to use NRI (Nurses’ Retention Index) developed by Cowin (2002) as a tool for Kim Min-jung (2006). Results: Factors affecting nurses’ retention were analyzed. The analysis showed that retention intention was positively affected by self-efficacy (β=.24, p<.001), sense of calling (β=.23, p<.001) and annual wage (β=.21, p=.001) and negatively affected by workplace bullying (β=-.16, p=.008) on a statistically significant level, with an explanatory power of 28.6%.

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

Improving the subcritical water extraction of flavonoids narirutin and hesperidin from Citrus unshiu peel by pulsed electric fields

Hui-Ju Kim,Mi-Ri Kwon,Hye-Ji Kang,Na-Yeon Kim,Hee-Jeong Hwang,Min-Jung Ko,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Citrus fruit is important source of flavonoids, mainly flavanones which are narirutin and hesperidin. Those citrus flavonoids have been found to have health-related properties including antioxidant, anticancer, and anti-inflammatory. The main purpose of this study was to verify that the extraction of narirutin and hesperidin from Citrus peel can be more effective by combining pulsed electric field (PEF) pre-treatment and subcritical water extraction (SWE). Citrus unshiu peels were treated with PEF under conditions of electric field strength (3 kV/cm) and times (1 and 2 min). Subsequent SWE was conducted by using a Dionex Accelerated Solvent Extractor (ASE, Model 350) at extraction temperature 170°C for 10 min. The total flavonoids content was measured by using the aluminum chloride colorimetric method and the antioxidant capacity was analyzed by the Ferric reducing antioxidant power (FRAP) assay using spectrophotometer. The concentrations of narirutin and hesperidin were increased as PEF pre-treatment time increased. The highest concentrations of narirutin and hesperidin were 13.41 mg narirutin/g dry citrus peel and 141.16 mg hesperidin/g dry citrus peel at PEF pre-treatment condition of 3 kV/cm and 2 min. The total flavonoids contents of the extracts increased 105.2% and 123.1% for citrus peel PEF treated at 1 and 2 min, respectively. In addition, compared to the untreated sample, PEF pre-treatments of 1 and 2 min increased the antioxidant capacity of the extracts 109.2% and 160.8%, respectively. Therefore, the results demonstrate the potential of PEF pre-treatment to improve the SWE of flavonoids from citrus unshiu peel.

원산지별 감초추출물의 항산화활성 증가를 위한 효율적인 추출조건 탐색

하지훈 ( Ji Hoon Ha ),이혜미 ( Hye Mi Lee ),권순식 ( Soon Sik Kwon ),김해수 ( Hae Soo Kim ),김문진 ( Moon Jin Kim ),전소하 ( So Ha Jeon ),정유민 ( Yoo Min Jeong ),황준필 ( Jun Pil Hwang ),박종호 ( Jong-ho Park ),최영기 ( Yung-key 대한화장품학회 2013 대한화장품학회지 Vol.39 No.4

본 연구에서는 한국 및 중국산 감초(Glycyrrhiza uralensis), 그리고 우즈베키스탄산 감초(Glycyrrhiza glabra)를 대상으로 추출용매, 추출온도, 추출시간 등 추출조건별 추출물을 제조하고 이들 추출물들의 추출 수율과 항산화 활성을 비교하여 최적의 추출조건을 선정하였다. 항산화 활성 중 자유 라디칼(1,1-phenyl-2-pic-rylhydrazyl, DPPH) 소거활성은 85% 에탄올로 60 ℃에서 6 h 동안 추출한 조건에서 한국 감초로부터 얻은 추출물이 가장 높은 활성(46.05%)을 나타내었다. 루미놀 발광법을 이용한 추출물들의 총 항산화능 측정 실험과 피부 광노화에 중요한 ¹O₂으로 유도된 세포막 손상에 대한 추출물들의 세포 보호 효과를 측정한 실험 모두에서도 위의 조건에서 가장 높은 항산화 활성을 나타내었다. 특히, 한국 감초는 τ₅₀이 116.4 min으로 비교 물질인(+)-α-tocopherol (28.5 min)보다 약 4 배나 높은 세포 보호 효과를 나타내었으며, 추출 수율은 18.75%로 우즈베키스탄 및 중국 감초보다 각각 1.2 배 및 2.5 배의 추출 수율을 나타내었다. 따라서, 본 연구 결과는 항산화 소재로 화장품에 응용하기 위하여 감초로부터 추출물을 얻기 위한 최적의 조건은 85% 에탄올로 60 ℃에서 6시간 동안 추출하는 것임을 보여주었다. In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent, temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra (G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidative activities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scav-enging activity (46.05%) under the extraction condition of 85% ethanol at 60 ℃ for 6 hours. The prominent ROS (reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protective effect against ¹O₂ induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (τ₅₀ = 116.4 min) than (+)-α-tocopherol (τ₅₀ = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2 times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extraction condition of 85% ethanol at 60 ℃ for 6 hours is optimal to prepare licorice extracts, which can be applicable as antioxidative cosmetic materials.