물리적 처리에 의한 강력분 밀가루 Gliadin의 항원성 변화

강보경(Bo-Kyeong Kang),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박시우(Si-Woo Bark),박원민(Won-Min Pak),김보람(Bo-Ram Kim),안나경(Na-Kyung Ahn),최연욱(Yeon-Uk Choi),최정수(Jung-Su Choi),최호덕(Ho-Duk Choi),안동현(Dong-Hyun A 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.4

본 연구에서는 가압가열 및 microwave 처리가 gliadin의 항원성에 미치는 영향을 살펴보기 위해 강력분에 가압가열과 microwave를 단독 또는 병행으로 처리하여 Ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였다. 가압가열 처리의 경우 시간이 길어질수록 IgG와의 결합력이 감소하였으며, 특히 50분 처리구에서 약 87%로 가장 낮은 결합력을 보였다. 또한 SDS-PAGE와 immunoblotting 결과에서도 무처리구에서 강하게 보였던 gliadin band가 가압가열처리에 의해 거의 소실되고 항체와 반응하지 않았다. 가압가열 및 microwave를 병행 처리 시도 마찬가지로 gliadin의 결합력이 감소하였으며, 처리구 중 가압가열 50분, microwave 5분 처리구에서 약 93%로 가장 낮은 결합력을 보였다. 반면 microwave를 단독으로 처리한 경우에는 일부 단백질의 변화는 관찰되었으나, 항원성 감소에는 효과가 없음을 확인하여 단백질 변화가 항원성에는 큰 효과를 준 것 같지 않다. 이상의 결과를 통해 가압가열 단독 처리 및 가압가열 및 microwave의 병행처리 시 gliadin의 항원성이 감소함을 확인하였다. This study was conducted to evaluate the effects of physical treatments on the antigenicity of gliadin in strong wheat flour. Strong wheat flour was treated with an autoclave (5, 10, 30, 50 min), a microwave (1, 5, 10 min), or both (10, 30, 50 min/ 5, 10 min), followed by SDS-PAGE, immunoblotting, and Ci-ELISA using anti-gliadin IgG. The results indicated that the binding ability of IgG to gliadin in strong wheat flour slightly decreased after autoclaving or autoclaving/microwaving. In particular, the binding ability was reduced to about 87% after autoclaving for 50 min and to 89% after autoclaving/microwaving (50/5 min). In addition, gliadin bands in the 50 min autoclaved group disappeared in both SDS-PAGE and immunoblotting. On the other hand, the antigenicity of gliadin was unaffected by microwaving alone. In conclusion, the results of this study suggest that autoclaving may reduce the antigenicity of gliadin in strong wheat flour.

총경동맥 폐쇄시간에 따르는 국소 뇌혈류 변화 : 실험적 연구 An Experimental Cat Model

강준기,성태경,조병일,백민우,김문찬,허춘웅,하영수,송진언 대한신경외과학회 1983 Journal of Korean neurosurgical society Vol.12 No.3

The microvasculature of the brain is also quite susceptible to ischemic insult, and substantial portions of the brain are not reperfused after restoration of the blood supply following overtime of critical ischemic periods. The purpose of this series of experiments was to determine the effects of ischemia on subsequential regional cerebral blood flow measurements and cortical electric activities following reperfusion after ischemia and also to define the proper time of vascular occlusion without irreversible neural damage. Cerebral ischemia was induced in cat by bilateral common carotid occlusions for periods of 10, 30, to 60 minutes, and the blood supply was reperfused for 3 hours after clamp-off. Regional cerebral blood flow(rCBF) was measured by hydrogen clearance technique following ischemia, restoration of blood supply and electroencephalogram recovery could be predicted according to the rCBF. Forty adult cats weighing 2.7 to 4.0㎏ were used in this study. The animals were divided into 4 groups of 10 cats each : normal control, 10 min-clamped, 30 min-clamped, and 60 min-clamped groups. The results obtained were as follows : 1) The mean rCBF was 24.6±7.0㎖/100g/min in control group. 2) Bilateral carotid occlusions resulted in a reduction of the rCBF(12.4±4.1㎖/100g/min) to 50% of control flow on both hemispheres. 3) Sequential changes of the rCBF after reperfusion : (1) There was restored the rCBF(21.3±5.1㎖/100g/min) to control flow in the 10 minutes-clamped group. (2) There was a 85% recovery of control flow in the 30 minutes-clamped group. (3) There was a only 25% recovery of control flow in the 60 minutes-clamped group. 4) A close correlation was found between cortical electrical activity and rCBF suggesting a threshold relationship. (1) The changes of cortical electric activity began to notice at rCBF less than 17.4±4.7㎖/100g/min. (2) The recovery of cortical electric activity noted at rCBF more than 10.2±2.3㎖/100g/min. 5) There was no evidence of ischemic involvement at the cortex, white matter and basal ganglia in the 10 minutes clamped group, but demonstrated a dense wedge shaped infarct at the cortex and uncus herniation in the 60 minutes clamped group. The rCBF and cortical electric activity restored to normal values in reperfusion within 10 minutes after occlusion of both common carotid arteries.

굴 스파게티 소스의 개발

강경태(Kyung Tae Kang),허민수(Min Soo Heu),김진수(Jin-Soo Kim) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.1

굴을 보다 효율적으로 이용하고자 신세대 기호에 맞는 굴 스파게티 소스의 제조를 시도하였다. 유기산, 색조, 점도 및 관능검사의 결과로 미루어 보아 스파게티 소스의 굴 최적 첨가량은 11%로 판단되었고, 저장성 부여를 위한 최적 F? value는 4분이라고 판단되었다. 최적조건에서 제조한 굴 스파게티 소스의 수분, 단백질, 지방 및 회분은 각각 71.2%, 2.8%, 6.9% 및 3.2%이었다. 굴 스파게티 소스의 관능검사결과 향과 조직감의 경우 시판 스파게티 소스보다 좋았으며, 색의 경우 차이가 없었다. 굴 스파게티 소스의 총 아미노산 함량은 2,532.2 ㎎/100 g이었고 주요 아미노산으로는 glutamic acid, aspartic acid 및 phenylalanine 등이었다. 굴 스파게티 소스는 칼슘/인의 비율이 1.88로 칼슘이 흡수되기 좋은 비율로 구성되었다. 유리아미노산 함량은 시판 스파게티소스(1,165.5 ㎎/100 g)가 굴 스파게티 소스(1,040.2 ㎎/100 g)에 비하여 높았고, taste value 또한 시판 스파게티 소스(189.35)가 굴 스파게티 소스(151.26)보다 높았다. Taste value의 결과로 미루어 보아 맛에 관여하는 주 아미노산은 aspartic acid와 glutamic acid로 판단되었다. The study was carried out to prepare spaghetti sauce with oyster (SSO) and the food components character-istics of the SSO were also compared to those of commercial spaghetti sauces (CSS). The optimal addition ratio of oyster for preparing SSO was 11% based on 100 g of SSO according to the results of organic acid content, Hunter color value, viscosity, and sensory evaluation. The reasonable F? value for the keeping storage of SSO was about 4 min. The proximate composition of SSO prepared under the optimal processing condition was 71.2% moisture, 2.8% protein, 6.9% crude lipid, and 3.2% crude ash. The results of sensory evaluation suggested that the quality of SSO was superior to that of CSS. However, there was no significant difference (p<0.05) in sensory evaluation on color between CSS and SSO. The total amino acid content (2,532.2 ㎎/100 g) of SSO was higher than that of CCS (2,305.7 ㎎/100 g). The contents of calcium and phosphorus of SSO were 25.7 ㎎/100 g and 48.7 ㎎/100 g, respectively. The calcium content/phosphorus content showed a suitable ratio for absorbing calcium. The total free amino content and the taste value were 1,040.2 mg/100 g and 151.26, respectively. The major taste?active amino acids were glutamic acid and aspartic acid.

Active Balancing of Li-Ion Battery Cells Using Transformer as Energy Carrier

Lee, Kyung-Min,Lee, Sang-Won,Choi, Yoon-Geol,Kang, Bongkoo Institute of Electrical and Electronics Engineers 2017 IEEE transactions on industrial electronics Vol.64 No.2

<P>A circuit for balancing Li-ion battery cells is proposed. This circuit requires one small transformer and N + 3 bilateral switches to equalize the charging states of N serially connected battery cells. The transformer works as an energy carrier, and the switches select two unbalanced cells that require an energy transfer from one to the other cell. The circuit was tested for a 12-cell Li-ion battery under static, cyclic, and dynamic charging/discharging conditions. Under static condition, the power-transfer efficiency was measured as 80.4% at a balancing power of 0.78W; two 4400-mA . h battery cells at a state of charge (SOC) = 70 and 80% were equalized after 78 min. The results of cyclic and dynamic charging/discharging conditions show that the circuit is appropriate for balancing the Li-ion battery cells for vehicles and energy storage systems.</P>

치과용 임플란트 적용을 위한 항균력을 가진 티타늄 표면의 평가

강민경 ( Min Kyung Kang ),문승균 ( Seung Kyun Moon ),김경남 ( Kyoung Nam Kim ) 한국치위생과학회 2011 치위생과학회지 Vol.11 No.5

The aim of this study was to evaluate antibacterial effect of Cl coated titanium. To coat the Cl on the titanium, first, the titanium was modified by blasting treatment with hydroxyapatite and alumina powder. Anodization process was completed using electrolyte solution of 0.04 M β-glycerol phosphate disodium salt n-hydrate, 0.4 M calcium acetate n-hydrate and 1 M NaCl on the condition of 250 voltages for 3 min. Surface morphology and elements` observation were performed with scanning electron microscopy and energy dispersive spectroscopy and surface profiler was used to analyze the surface roughness. Antibacterial effect was evaluated by film adhesion method. The anodized titanium after blasting showed dimpled surface contained the Cl. Surface average roughness of these surfaces had significantly higher compared to polished titanium. Result of antibacterial test showed that anodized titanium after blasting had an enhanced antibacterial effect compared to the polished titanium. Therefore, these results suggested that titanium contained Cl by anodization after blasting had a rough surface as well as antibacterial effect.

생식용 굴(Crassostrea gigas) 작업장의 위생안전성에 대한 모니터링

강경태 ( Kyung Tae Kang ),박선영 ( Sun Young Park ),최종덕 ( Jong-duck Choi ),김민주 ( Min Joo Kim ),허민수 ( Min Soo Heu ),김진수 ( Jin-soo Kim ) 한국수산과학회 2017 한국수산과학회지 Vol.50 No.2

This study assessed the safety of raw oysters (Crassostrea gigas) for consumption during processing in a processing plant. Bacterial contamination (e.g., viable cell counts, coliform groups, and pathogenic bacteria) and chemical contamination (e.g., heavy metals and shellfish toxins) were measured on raw oysters, a processing equipment, employees, and work areas. No total mercury, lead, paralytic shellfish poison, diarrheic shellfish poison, or norovirus was detected in any post-harvested oyster samples. However, the cadmium level ranged from 0.1-0.2 mg/kg. The viable cell count and Escherichia coli and coliform group levels in post-harvested oysters ranged from 4.00-4.54 log CFU/g, ND-210 MPN/100 g, and 110-410 MPN/100 g, respectively. The viable contaminating cell counts on employees, equipment, and work areas were in the range of 0.90-3.46 log CFU/100 cm². Airborne bacteria in the work areas ranged from 0.60 to 1.81 log CFU/plate/15 min. Thus, no significant health risks were detected in the processing plant.

대장직장 종양에 대한 내시경 점막하 절제술의 연대순 임상 결과와 학습 곡선

이상민 ( Sang Min Lee ),김은수 ( Eun Soo Kim ),박경식 ( Kyung Sik Park ),조광범 ( Kwang Bum Cho ),김동춘 ( Dong Choon Kim ),강유진 ( Yu Jin Kang ),이유진 ( Yoo Jin Lee ),이정민 ( Jung Min Lee ),최은성 ( Eun Sung Choi ),최재혁 ( Ja 대한소화기학회 2014 대한소화기학회지 Vol.64 No.4

Background/Aims: Endoscopic submucosal dissection (ESD) is an effective procedure for en-bloc curative resection of the colorectal tumor. As it requires high technical skills and experience in therapeutic endoscopy, it is important to understand learning curve of ESD technique. The aim of this study was to retrospectively describe the clinical results of ESD and to evaluate learning curve for the ESD of colorectal tumors. Methods: A total of 90 patients with 90 colorectal neoplasms, who had undergone ESD at a tertiary referral hospital from July 2009 to December 2012, were enrolled. The ESD was performed by a single endoscopist. All ESD cases were divided into three periods: first, cases 1-30; second, cases 31-60; and third, cases 61-90. Results: The en-bloc resection rates in third period (100%) was significantly higher than that of the first (93.3%) and second period (80%) (p=0.025). The perforation rate in third period (0%) also significantly decreased compared with that of the first (13.3%) and second period (20%) (p=0.032). To calibrate the difference of tumor size among periods, proficiency was calculated, as the procedure time per specimen area (min/cm2). The proficiency in third period (4.3) was significantly shorter than that of the first (16.8) and second period (10.2) (p=0.004). Conclusions: The learning curve of colorectal ESD in our study shows that at least 60 cases of ESD have to be conducted to acquire sufficient skill of degree without perforation. (Korean J Gastroenterol 2014;64:198-205)

Pharmacokinetics and Biodistribution of a pGT2-VEGF Plasmid DNA After Administration in Rats

Son, Mi-Kyung,Choi, Jae-Hoon,Lee, Dong-Sop,Kim, Chae-Young,Choi, Seul-Min,Kang, Kyung-Koo,Byun, Jonghoe,Kim, Duk-Kyung,Kim, Byong-Moon Lippincott Williams Wilkins, Inc. 2005 Journal of cardiovascular pharmacology Vol.46 No.5

Intramyocardial administration of gene therapy vectors expressing angiogenic factors have been attempted as an alternative to conventional surgical methods for the management of myocardial ischemia. In this study, we have developed the pGT2-VEGF, a plasmid DNA vector expressing human VEGF165, for the management of ischemic cardiovascular disease and investigated in vivo pharmacokinetics and tissue distribution of pGT2-VEGF after intramyocardial and intravenous administration in rats. A high concentration of pGT2-VEGF was observed in the heart after intramyocardial injection of 300 μg, which is in line with the assumption that direct intramyocardial delivery enables extended localization at the administration site. Leakage of the pGT2-VEGF to the blood circulation was observed after intramyocardial injection, with an area under the curve (AUC) of 3.8 μg min/mL, as compared with 37.3 μg min/mL after intravenous injection of the same dose. The pGT2-VEGF concentration in blood peaked at 5 minutes after intramyocardial administration and declined rapidly to undetectable levels by 2 hours post-administration. In tissue distribution studies, pGT2-VEGF peaked at 5 minutes post-administration in various organs but was undetectable at 2 hours in all organs except heart, lung, and liver. Taken together, the results suggest that intramyocardial-delivered pGT2-VEGF was degraded rapidly in vivo and mainly persisted in target tissues, the heart. In addition, intramyocardial-administered pGT2-VEGF was expressed for longer periods than the persistence of the pGT2-VEGF plasmid DNA in a target tissue. Therefore, a direct myocardial injection of pGT2-VEGF might be useful for local therapeutic angiogenesis.

P278 : Nonablative fractional laser as a tool to facilitate skin penetration of 5-aminolevulinic acid with minimal skin disruption

( In Jung Kang ),( Jeong Hwee Choi ),( Min Jae Gwak ),( Hee Kyeong Lim ),( Mu Hyoung Lee ),( Min Kyung Shin ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Effective penetration of photosensitizer is an essential step in photodynamic therapy (PDT). There have been trials of several methods, including laser treatment, to facilitate prompt and sufficiently deep transdermal drug delivery. Objectives: This study was performed to evaluate the effects of nonablative fractional laser pretreatment on aminolevulinic acid (ALA) penetration of the skin. Methods: Twelve identical treatment areas of 1 × 1 cm2 on the backs of 10 healthy male subjects were mapped. Each area received laser treatment with a different energy and incubation with ALA for different times. Laser treatment was performed with a 1550 nm fractional erbium glass laser, and the laser energy was set to 20 and 50 mJ with a spot density of 50/cm2. ALA incubation time was set to 30, 60, or 180 min. Porphyrin fluorescence was measured. Results: Nonablative fractional laser-pretreated areas showed significantly increased porphyrin fluorescence compared to non-pretreated areas. Laser energy strength and ALA incubation time were positively correlated with ALA absorption. Conclusion: Nonablative fractional laser treatment effectively enhanced ALA skin penetration. Pretreatment with a nonablative fractional laser can be used for ALA-PDT to achieve higher therapeutic efficacy and shortened ALA incubation times with minimal skin barrier disruption compared to ablative laser.

수확 후 산채류의 미생물 제어를 위한 이산화염소수와 유기산 및 Blanching 병합 처리

강지훈(Ji Hoon Kang),박신민(Shin Min Park),김현규(Hyun Gyu Kim),손현정(Hyun Jung Son),이가연(Ka Yeon Lee),강길남(Kil-Nam Kang),박종태(Jong Tae Park),송경빈(Kyung Bin Song) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.2

수확 후 산채류의 미생물학적 안전성을 확보하기 위해 선정된 산채류인 취나물과 곤드레에 이산화염소수와 유기산 용액 병합 처리 및 이산화염소수, 유기산 용액, blanching 병합처리 후 미생물 제어 효과를 비교 분석하였다. 50 ppm 이산화염소수와 0.5% citric acid 용액의 병합 처리는 취나물과 곤드레의 총 호기성 세균 수를 2.80~3.64 log CFU/g, 효모 및 곰팡이 수는 2.02~2.67 log CFU/g 감소시켰다. 50 ppm이산화염소수와 0.5% fumaric acid 용액의 병합 처리 후 총 호기성 세균 수는 대조구와 비교하여 3.62~3.82 log CFU/g 감소하였으며, 효모 및 곰팡이의 경우에는 2.47~3.02 log CFU/g 만큼 감소하여 이산화염소수와 citric acid 용액의 병합 처리보다 fumaric acid 용액과의 병합 처리가 더 효과적인 병합 처리 조건이라고 생각된다. 이산화염소수와 fumaric acid 병합 처리 후 blanching 처리된 취나물의 총 호기성 세균 수는 대조구보다 5.12 log CFU/g 더 낮게 검출되었으며, 효모 및 곰팡이는 검출되지 않았다. 곤드레의 경우에도 효모 및 곰팡이는 검출되지 않았으며, 총 호기성 세균 수는 대조구와 비교하여 4.59 log CFU/ g 감소하였다. 따라서 본 연구 결과 이산화염소수와 유기산 용액 전처리 후 blanching 병합 처리가 산채류의 미생물학적 안전성을 확보하는 가장 효과적인 방법이라고 판단된다. To improve the microbiological safety of wild vegetables after harvest, Aster scaber and Cirsium setidens Nakai were treated with combinations of 50 ppm aqueous chlorine dioxide (ClO₂)/0.5% citric acid or fumaric acid, and 50 ppm ClO₂/0.5% fumaric acid/blanching at 90°C for 2 min. Combined treatment of 50 ppm ClO₂ and 0.5% citric acid reduced populations of total aerobic bacteria, yeast, and molds in Aster scaber and Cirsium setidens Nakai by 2.80∼3.64 and 2.02∼2.67 log CFU/g, respectively, compared to those of the control. Combined treatment of 50 ppm ClO₂ and 0.5% fumaric acid reduced total aerobic bacteria, yeast and molds populations by 3.62∼3.82 and 2.47∼3.02 log CFU/g, respectively. Based on the results, combined treatment of ClO₂ and fumaric acid was more effective in controlling microorganisms in the wild vegetables than either ClO₂ or citric acid. In addition, combined treatment of ClO₂/fumaric acid/blanching reduced the populations of total aerobic bacteria by 4.59∼5.12 log CFU/g, and populations of yeast and molds were not detected by treatment. These results suggest that combined treatment of ClO₂/fumaric acid/blanching is the most effective method for improving microbiological safety of wild vegetables after harvest.