자바리, Epinephelus bruneus의 난 발생과 부화에 미치는 수온의 영향

양문호,최영웅,정민민,구학동,오봉세,문태석,이창훈,김경민,한석중,Yang, Moon-Ho,Choi, Young-Ung,Jung, Min-Min,Ku, Hag-Dong,Oh, Bong-Sae,Moon, Tae-Seok,Lee, Chang-Hoon,Kim, Kyong-Min,Han, Seock-Jung 한국발생생물학회 2007 발생과 생식 Vol.11 No.2

This study examined temperature effect in egg development and hatching of longtooth grouper, Epinephelus bruneus. Fertilized embryos was not growth after morula stage at $15^{\circ}C$, at 18, 21, 24 and $27^{\circ}C$, the required time from fertilized embryos to hatching were 70 h. 30 min., 44 h. 10 min., 29 h. 10 min. and 24 h. 30 min., respectively. The hatching rates at $24^{\circ}C$ were higher than the other conditions and the hatching was not occurred at $15^{\circ}C$. These results suggest that the water temperature range of egg development and hatching was $18{\sim}27^{\circ}C$. 이 연구는 실내 사육수조에서 자연산란 후 수정된 난을 대상으로 수온에 따른 난 발생속도와 부화율을 조사하였다. 부화에 이르기까지 각 수온조건에서 소요된 시간은 $15^{\circ}C$에서 상실기 이후 발생이 진행되지 않았고, 18, 21, 24, $27^{\circ}C$에서 각각 70시간 30분, 44시간 10분, 29시간 10분 그리고 24시간 30분이 소요되었다. 부화율은 $15^{\circ}C$에서 0%였고, 18, 21, 24 그리고 $27^{\circ}C$에서 각각 $8.3{\pm}1.2%$, $18.0{\pm}6.2%$, $24.0{\pm}4.0%$ 그리고 $17.0{\pm}7.2%$로 뚜렷한 차이 없이 $24^{\circ}C$에서 다소 높았고 21와 $27^{\circ}C$에서 비슷한 경향이었으며 $18^{\circ}C$에서 가장 낮게 나타났다. 이러한 결과에서 자바리의 난발생 수온범위는 $18{\sim}27^{\circ}C$로 제안된다.

본태성 고혈압 환자의 적혈구 Na+-Li+ Countertransport 와 적혈구막 지방산 구성에 대한 연구

이영우,고경수,고재준,박경수,김원배,한은경,김성연,조보연,이홍규,고창순,민현기 한국지질학회 1991 韓國脂質學會誌 Vol.1 No.1

Since the changes of Na^+-Li^+ countertransport (SLC) Vmax in essential hypertension have been reported. it has been known that hyperlipidemia could affect SLC independently of blood pressure. To reveal the relation between SLC Vmax and RBC membrane fatty acid conposition in the hypertensive patients, we divided patients into two groups according to their levels of SLC Vmax of erythrocytes(elevated SLC Vmax: n=4, normal SLC Vamx: n=6). The results were as follows: 1) There was no difference in body mate index, waist-hip ratio, blood pressure, serum cholesterol level between two groups. Triglyceride were higher in elevated group (278.0±180.0 ㎎/㎗) than normal group (124.3±60.7 ㎎/㎗) but there was no statistical significance. HDL-cholesterol levels were significantly lower in elevated group (32.0±3.6 vs. 44.8±8.9 ㎎/㎗) and free fatty acid concentrations, intracellular (RBC) Na^+ concentrations were similar between two groups. 2) The results of oral glucose tolerance test showed no differences in the glucose and insulin area, however the incremental insulin areas of elevated group (108.8±31.3, μU. min/㎖×10²) were significantly higher compared with those of normal group (56.3±20.7 μU. min/㎖×10²). 3) In the elevated group, palmitic acid proportions of erythrocytes (22.1±0.7%) were higher than those of normal group (20.1±0.7%) and SLC Vmax showed significant correlation (r=0.81, p$lt;0.01) with palmitic acid proportion. These results suggest that the abnormal lipid metabolism may change the RBC Na^+ transport system through the alterations of RBC membrane fatty acid composition.

High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb

( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

알코올이 Dynamic Posturography에 미치는 영향

이진춘,고의경,장민호,이병주,백무진,노환중,왕수건,전경명 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.2

가토에서 Vecuronium bromide 의 용량에 따른 근육이완 효과

김동원,이동호,민경호,전세웅,유희구,김경헌,승익상,김교상,서정국 대한마취과학회 1987 Korean Journal of Anesthesiology Vol.20 No.6

The introduction of muscle relaxants was an epoch making event in the development of clinical anesthesia and their use has hecame essential to anesthesia practice. Vecuronium, one of the newest muscle relaxants, many advantages; it is .apid acting, non-accumulating, has minimals side reactions and is promptly antagonized by anticholinesterases. However, its pharmacological effect, like other conventional muscle relaxants depends upon the dose and is influenced br many factors including body temperature, blood flow, interaction with other drugs, antibiotics, diuretics and so forth, To obtain a dose·response relationship which is important for clinical use, the effect of the vecuronium in various doses on the neuromuscular block in rabbits was investigated. The results were as follows. 1) In the vecuronium 0.025 mg/kg group, maximal twitch depression was varied between 75 to 92% with a mean value of 84,9%. The spontaneous recovery index was 141.0 seconds (2.35 min). 2) In the vecuranium 7.75 mg/kg group, thmean maximum twitch depression and spontaneous recovery index were 98.4% and 218.6 seconds (3.64 min.) respectively. 3) In the vecuronium 7.1 mg/kg group, maximum twitch depression was 100% and the spontaneous recovery index was markedly prolonged to 627.8 seconds (10.35 min).

고등어(Scomber japonicus ) 치어의 마취제로서 Clove oil, MS-222 및 2-Phenoxyethanol의 평가

한석중 ( Seock Jung Han ),김경민 ( Kyong Min Kim ),최낙중 ( Nack Jung Choi ),구준호 ( Jun Ho Koo ),박충국 ( Chung Kug Park ),이원교 ( Won Gyo Lee ),지승철 ( Seung Chul Ji ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6

The efficiency of clove oil, MS-222, and 2-phenoxyethanol was evaluated as anesthetics in juvenile Scomber japonicus . Stage A5 of anesthesia was assumed to be sufficient for conducting routine aquaculture procedures in less than 3 min, with recovery (stage R5) in less than 5 min. The lowest effective doses of the three anesthetics were 50 mg L-1 clove oil (anesthetic time of 71.3 s and recovery time of 167.0 s), 100 mg L-1 MS-222 (anesthetic time of 70.7 s and recovery time of 115.7 s), and 400 mg L-1 2-phenoxyethanol (anesthetic time of 86.7 s and recovery time of 95.0 s). Anesthetic times decreased with increasing doses for all three anesthetic agents, and fish anesthetized with clove oil exhibited the longest recovery times. After 30 min, the highest plasma cortisol and lactate levels were detected with the use of clove oil, whereas the lowest values were observed with 2-phenoxyethanol. In addition, high glucose levels were maintained during recovery with clove oil, but the treatments did not significantly differ. The most effective of the three anesthetic agents was 2-phenoxyethanol, although all were considered acceptable for use in cultures of juvenile Scomber japonicus

일부농촌지역의 농약사용실태 및 농약중독 요인 조사

이경민,송주희,장재혁,심수정,강양화,안재경,이숭호,박미영,정문호 대한보건협회 2002 대한보건연구 Vol.28 No.4

Object The usage of agricultural chemical is on the increasing. Use of pesticides has increased agricultural production. However, negative externalities from such use have increased too. Pesticide poisoning is a major public problem. The purpose if this study is to find out the relationship between keeping the safety rules or protective equipments or attending safety education or dose of pesticides use , and poisoning experience. Methods For Gu, H.M. and Pack, S.G. study , we obtained questionnaire data concerning pesticides poisoming. A person interviews on 203 were conducted in two villages in Sinmeari and woulbonri Chunchun , Kangwon province, July 2002. Result 1. 144 people(70.9%) have sprayed pesticides and the rata of experiences of pesticide poisoning was 26% among 144. Spraying time of pesticides was 1-2 hours in 63.9% of farmers. Rate of using the protective equipment was 25.7% (protective clothes), 49.3% (Mask), 48.6 (Gloves), 7.0 (Protective glasses), 64.6% (boots) respectively. 2. Chi-square and multiple logistic regression analysis showed that Duration of spraying per day (<2hr) and using of mask(protective equipment)are significantly association with poisoning experience.(x^2=5.2684 p=0.0217, CI=0.140-0.5853 OR=0.346 p=0.0211 respectively). But no association between poisoning experience and keeping safety rule, attending of safety education. 3. Spraying pesticides. Duration of spraying pesticides, reading manuals and no spraying at fatigue (keeping safety rule) are significantly association with symptom experience(x^2=14.0621 p=0.0002, x^2=7.0639 p=0.0079, CI01.395-10.950 OR=3.908 p=0.0095, CI=0.101-0.726 OR=0.271 0.0094 respectively). But no association between symptom experience and protective equipments, attending of safety education.

완전 생분해성과 광분해성을 갖는 필름에 대한 연구

이상일,서석훈,홍경민,신용섭,장상희,신부영 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1

Bio-and-photodegradable polymer composites were prepared by blending biodegradable aliphatic polyester and photodegradable master batch(M/B). Photodegradability, biodegradability, thermal properties and mechanical properties were investigated. The biodegradability of EnPol(G4600) composites decreased wish increasing the amount of M/B, while that of EnPol(G8000) composites increased. Tensile strength and elongation decreased with increasing the time of outdoor exposure and the amount of M/B but modulus comparatively maintained. The mechenical properties decreased as the content of M/B increased, while the melting temperature unchanged.

나라꽃 무궁화의 어문학적 고찰

鄭珉,李慶善,李明奎 한양대학교 한국학연구소 1986 韓國學論集 Vol.9 No.-

"Mugunghwa", or Hibiscus syriacus is regared as the natinal flower of korea. Every nation generally has the national flower. It represents characteristics of the nation and the race. Therefore it is their symbol. The relatin of "Mugunghwa" and our race has been a longtime in history. It goes was back to about two thousand years ago in literature. Particulaly, in the Japanese imperialism period when our race was suffered, "Mugunghwa" is a unfogettabl, precious, and proud flower to our race. But nowadays, our interest on this flower is very faint. The reason is not only the intentional fabrication of the idea by Japanese imperialism but also the deficiency of our understanding and affection on it. In this study, firstly, the relation of "Mugunghwa" and our race was examined through the research on the old literature. And in the third chapter, names of "Mugunghwa" were linguitically examined through the research on the old literature and dialetcts. As the result, we know that the word "Mugunghwa" is derived from korean language. The fact that our literary works is silent on "Mugunghwa" has been the obstacle of the study on "Mugunghwa". In the fourth chapter, I divide songs and literary works on "Mugunghwa" into thirteen articles, and explanin them. Thus I know our racial affection on "Mugunghwa" in about seventy works of the Korean verse or shijo, 時調, the Chinese poem and Poetry and novels in Korean civilzed period. Finally, in the fifth chapte, I synthesized and examined pro-blems concerning "Mugunghwa". This is to discuss the adequacy of argument on chage of the national flower and the reasonableness of "Mugunghwa" as the national flower that has been frequently sugge-sted. The aim of this study is synthetically to examine problems on "Mugunghwa". Through this study, we will know the feature of "Mugunghwa" as the national flower and the concrete method of affection for it. This knowledge is connected wth our patiotism and love for our race.

ECAP 공정을 거친 1050 Al 합금 판재의 전기화학 특성에 관한 연구

정민경,최윤석,김정구,김영만,이재철,석현광 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.8

The effect of equal channel angular pressing (ECAP) pass number on the electrochemical properties of AA 1050 (UNS A91050) in 0.1M Na₂SO₄ solution containing 100 ppm chloride were investigated by electrochemical techniques (potentiodynamic test, potentiostatic test, electrochemical impedance spectroscopy (EIS) and surface analyses (OM, SEM, EDS). The ECAPed specimens that have a various numbers of passes from N=l to N=5 were selected for electrochemical experiments in this study. The results of potentiodynamic test indicated that the breakdown potential increased with increasing the ECAP pass number. EIS measurements also showed that the corrosion resistance of the AA 1050 increased with increasing the ECAP pass number at the passivation and breakdown states. The results of surface analyses (SEM, EDS) showed that silicon-containing impurities were observed on the surface of the alloy. It was found that the size of these Si-containing impurities decreased with increasing the ECAP pass number. The Si-containing impurities induced the microgalvanic reaction(i) between the Al matrix and the Si-containing mixed oxide and (ii) between the Al matrix and the Si-containing impurities. Due to its ultrafine-grained microstructure, ECAPed AA 1050 had smaller Si-containing impurities, and reduced microgalvanic currents. Thus, in the case of ECAPed AA 1050, it has increasing corrosion resistance with increasing ECAP pass number because of its small cathodic impurities.