Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

대장내시경검사의 전처치로서 항콜린제 사용에 관한 연구 : 전향적, 이중맹검법으로

박경남,한동수,이민호,최호순,박준용,손주현,이오영,함준수,전용철,송승찬,기춘석,윤병철,이종희 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.3

Background: Use of antispasmodic medication prior to colonoscopy is controversial but someone believes antispasmodic may improve visualization of colonic mucosa and ease colonoscope insertion. So, we designed a study to assess the effect of premedication with the antispasmodic, hyoscine-N-butyl bromide(Buscopan') on the performance of colonoscopy. Methods: This study was prospective, double blinded, randomized, controlled study, One hundred three consecutive patients were randomized to receive intravenous buscopan lml(n=52) or placebo(n=51) combined with our standard initial medication(me- peridine 50 mg and midazolam 2 mg). Insertion of colonoscopy was timed, and 100 mm visual analogue scales (VAS) were used for asscssing difficulty of procedure, colonic motility, frequency of positional change, frequency of external compression, difficulty of assistance and degree of discomfort experienced by the patients. Results: There were no significant differences of intubation time between buscopan group(mean time, 7.23 min., range 2~15) and placebo group(7.07 min., range 3-25), (p=0.83) and withdrawal time between buscopan group (6.46 min., range 2-22) and placebo group(6.76 min., range 2 25), (p=0.69). Also, there was no significant differences in intubation time between males and females(buscopan; males 7.00 min., females 7.60 min., p=0.34, placebo; males 7.0~5 min., females 7.08 min., p 0.44). The VAS scores checked by endoscopist(p=0.29), assistant(p=0.32) and patient (p=0.15) were not significantly different in both groups. There were no significant differences in intubation time, VAS scores nf endoscopist, assistant, and patients. Conclusion: Premedication with intravenous bu.opan has no advantage on colonoscopy procedure. Use of antispasmodic medication prior to colonoscopy was not considered as recommendable agent.

적송 (Pinus densiflora) 림내 송이 (Tricholoma matsutake) 발생지와 미발생지의 토양 균류의 수직 분포

송현순,민강희 한국균학회 1991 韓國菌學會誌 Vol.19 No.2

The vertical distribution of the fungal population for the soil samples from two sites of producing and nonproducing of Tricholoma matsutake, song-yi mushroom, were examined at Yang-yang and Myung-joo, Gangweon province . By the dilution plate method, a total number of propagnles of fungi per gram of soil was observed to be low at the song-yi producing sites but high at the song-yi nonproducing sites under the communities of Pinus densiflora. The tendency of the number of fungal propagules were decreased with the increasing vertical depth. In the incubation method st 42℃, six genera and nineteen species of the fungi were isolated from two sites; Aspergillus fumigatus, Acremonium sp., Talaromyces stipitatus, Penicillium lilacinum, P. axalicum and Westerdykella multisporo. The most dominant species by this method was A. fumigates. From heat treatment method at 70℃, seven genera and nineteen species were isolated; Aspergillus fumigatus, Alternaria alternata, Neurospora sitophila and Mucor sp.. In the ethanol treatment method, one genera and one species was isolated Mortierella sp.. From the three isolation methods, it was found that the total number of the soil fungi and the frequency of species appeared were the highest at the soil of upper layer whereas the lowest at the soils of lower layer in its vertical distribution.

GO-41 : Single port laparoscopic trans-umbilical extra-peritoneal para-aortic lympadenectomy

( Min Jong Song ),( Joo Hee Yoon ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

A 59 year old woman affected by squamous carcinoma of the cervix, FIGO stage IIB, with enlarged external iliac and common iliac lymph nodes at a CT scan with, was scheduled to extra peritoneal paraaortic lymphadenectomy to rule out para-aortic lymph nodes metastasis and decide treatment modality. A 2 cm vertical incision was made at the midline of umbilicus. Open access of umbilicus wall was performed and the surgeon`s index finger and 10mm indicator were inserted into retroperitoneal space to bluntly develop it. After the introduction of Glove port disposable single port device, 12 mm Hg pressure of carbon dioxide gas was insufflated into retroperitoneal space. A 5 mm, 30° laparoscope was inserted into the right lower port, dissector into the left upper port and harmonic scalpel into the left lower poert. After identification retroperitoneal structure such as the psoasmuscle, iliac vessels, and ureter, development of the retroperitoneal space was continued till the identification of the inferior mesenteric artery. The lymphadenectomy specifically targeted the left-sided infra-mesenteric para-aortic spaces to exclude para-aortic lymph node metastasis. The operation was performed successfully without complication and any placement of retroperitoneal drain. Operative time was 130 min. 16 lymph nodes were removed and no metastasis was detected. Ambulation and dietary intake was started in day 0. Patient was discharged on day two. This report suggests that single port laparoscopic trans-umbilical extra-peritoneal para-aortic lymphadenectomy for surgical staging of locally advanced cervical cancer is feasible. Furthermore this procedure may reduce the chance of port site hernia and allow the patients to be taken following treatment.

이산화티타늄계 광촉매를 이용한 톨루엔의 처리 특성

전의찬,송민종,김전희 동신대학교 환경연구소 2000 환경연구 Vol.5 No.1

각종 급ㆍ만성의 건강장해를 일으킬 뿐만 아니라, 광화학 반응에 의해 오존을 형성하는 것으로 알려진 휘발성유기화합물 중 대표적인 물질인 톨루엔을 광촉매산화법으로 처리할 경우 최적 처리조건을 분석하고자 광촉매의 결정구조, 광원의 세기, 전자수용체로서 산소의 유무 등에 따른 톨루엔의 처리효율을 분석하였다. 실험결과 루틸령 결정구조에 비해 아나타제형 결정구조를 갖는 이산화티타늄의 톨루엔 처리효율이 높게 나타났으며, 광원의 세기가 높을수록 전자수용체로서 산소가 존재할 경우 높은 처리효율을 나타내었다. 시간과 공간적인 제약으로 물, 오존 그리고 증기와 같은 다양한 전자수용체가 존재할 경우의 톨루엔의 처리효율을 파악하지 못하였으며, 온도, 습도 및 촉매활성에 영향을 주는 여러 가지 인자들을 대상으로 그것들의 변화에 따른 처리효율의 고찰을 못하였다. 앞으로 보다 효율적인 이산화티타늄계 광촉매의 제조방법 개발과 이산화티타늄계 광촉매를 이용한 다양한 VOC 처리방법의 개발이 요구된다. An available methods for abating VOC(including Toluene) are thermal or catalytic incineration, liquid absorption, solid adsorption, condensing, membranes, biodegradation and plasma discharge, but a number of defects are these VOC abating methods, including ; requires a auxiliary fuel, generate secondary pollutants(waste catalysts, waste absorbents, waste adsorbents, ozone, etc.), a large scale, etc.. But photoactive catalysts, when illuminated with UV-light, generate highly reactive radicals that can oxidize the organic contaminants in gas. One methods to increase the efficiency of the process, and thereby reduce the light energy requirement, is by developing more active catalysts. Because of, ball type TiO2 catalyst were obtained commercially and/or prepared in laboratory were examined for their photoacivity, and they are TiO2 added some annex(Al203, SiO2, KsO, P205, etc.). The organic compounds used to identify the best photocatalysis condition were toluene. This study also examined the impact of catalyst structure, light intensity, existence of oxygen as an electron acceptor and Toluene initial concentration on the reaction kinetics. Labratory experiments were performed to investigate TiO2 photocataysis for treating test gas contaminated with Toluene. and TiO2 photocatalysis efficiency for mineralization of Toluene is also evaluated. The photooxidation of Toluene was carried out in a photocatalytic reactor at room temperature. I performed Toluene photocatalytic oxidation experiments using a single-pass annular reactor. The organic-contaminated gas was prepared in teflon sampling bag(25L) and passing through photocatalytic reactor by vacuum pump which is located reactor outlet. The Toluene diluted with mixture air or nitrogen gas. Flow rates for test gas stream were controlled by micro flow meter going out the reactor. The reactor illuminated with 15W near-UV lamp, BLB lamp or Fluorescent lamp. The light intensity at the catalyst surface were estimated to be 0.8 to 6㎽ ㎝-2s-1. The inner cell of the reactor was packed with ball type titanium dioxide(φ 6㎜). Toluene was determined by GC-FID of gas samples taken from the a glass sampling bulb which was located at reactor outlet by gas-tight syringe. TiO2 photocataysis for treating test gas contaminated with Toluene. and TiO2 photocatalysis efficiency for mineralization of Toluene is also evaluated. Using the anatase type photocatalyst, Touene conversion rate is greater than that of rutile(86.1% conversion of 100ppmv Toluene using anatase type photocatalyst, 78% conversion of 100ppmv Touene using rutile type photocatalyst at 0.5L/min) Effect of light intensity on the rate of Toluene photocatalytic decomposition was investigated by three type lamps within the reactor. When light intensity to increase, the Toluene conversion rate to increase. Using the BLB lamp showed higher activity than the Fluorescent lamp, but UV lamp showed the hight photocatalytic activity(19.1% conversion of 100ppmv Toluene with Fluorescent lamp, 60.4% conversion of 100ppmv Toluene with BLB lamp, 86.1% conversion of 100ppmv Toluene with UV lamp at 0.5L/min). Experiments with added oxygen as an electron acceptor improved the reaction rate considerably(86.1% conversion of 100ppmv Toluene without oxygen, 62.5% conversion of same Toluene concentration with mixture air at 0.5L/min).

이산화티타늄계 광촉매를 이용한 벤젠의 처리 특성

전의찬,송민종,김전희 동신대학교 환경연구소 1999 환경연구 Vol.4 No.1

An available methods for abating VOC(including Benzene) are thermal or catalytic incineration, liquid absorption, solid adsorption, condensing, membranes, biodegradation and plasma discharge, but a number of defects are these VOC abating methods, including ; requires a auxiliary fuel, generate secondary pollutants(waste catalysts, waste absorbents, waste adsorbents, ozone, etc.), a large scale, etc.. But photoactive catalysts, when illuminated with UV-light, generate highly reactive radicals that can oxidize the organic contaminants in gas. One methods to increase the efficiency of the process, and thereby reduce the light energy requirement, is by developing more active catalysts. Because of, ball type TiO₂ catalyst were obtained commercially and/or prepared in laboratory were examined for their photoacivity, and they are TiO₂ added some annex(Al₂O₃, SiO₂, KsO, P₂O_(5), etc.). The organic compounds used to identify the best photo catalysis condition were benzene. This study also examined the impact of catalyst structure, light intensity, existence of oxygen as an electron acceptor and Benzene initial concentration on the reaction kinetics. Labratory experiments were performed to investigate TiO₂ photo catalysis for treating test gas contaminated with Benzene. and TiO₂ photo catalysis efficiency for mineralization of Benzene is also evaluated. The photo oxidation of Benzene was carried out in a photo catalytic reactor at room temperature. I performed BTX photo catalytic oxidation experiments using a single-pass annular reactor. The organic-contaminated gas was prepared in Teflon sampling bag(25L) and passing through photo catalytic reactor by vacuum pump which is located reactor outlet. The BTX diluted with mixture air or nitrogen gas. Flow rates for test gas stream were controlled by micro flow meter going out the reactor. The reactor illuminated with 15W near-UV lamp, BLB lamp or Fluorescent lamp. The light intensity at the catalyst surface were estimated to be 0.8 to 6mW cm-2s-1. The inner cell of the reactor was packed with ball type titanium dioxide(Ø 6mm). Benzene was determined by GC-FID of gas samples taken from the a glass sampling bulb which was located at reactor outlet by gas-tight syringe. TiO₂ photo catalysis for treating test gas contaminated with Benzene. and TiO₂ photo catalysis efficiency for mineralization of Benzene is also evaluated. Using the anatase type photo catalyst, Benzene conversion rate is greater than that of rutile(92% conversion of 100ppmv Benzene using anatase type photo catalyst, 80% conversion of 100ppmv Benzene using rutile type photo catalyst at 0.5L/min) Effect of light intensity on the rate of Benzene photo catalytic decomposition was investigated by three type lamps within the reactor. When light intensity to increase, the Benzene conversion rate to increase. Using the BLB lamp showed higher activity than the Fluorescent lamp, but UV lamp showed the hight photo catalytic activity(26% conversion of 100ppmv Benzene with Fluorescent lamp, 63.5% conversion of 100ppmv Benzene with BLB lamp, 92% conversion of 100ppmv Benzene with UV lamp at 0.5L/min). Experiments with added oxygen as an electron acceptor improved the reaction rate considerably(92% conversion of 100ppmv Benzene without oxygen, 66.1% conversion of same Benzene concentration with mixture air at 0.5L/min). The Benzene conversion rate begins to decrease while Benzene initial concentration begins to increase. We have thus concentrated our effects on the study of Benzene degradation kinetics in vary initial Benzene concentration.

암호화된 DB에서 대칭키 기반 검색기법 구현

정민경(Jung, Min-Kyoung),송희정(Song, Hee-Jeong),신승수(Shin, Seung-Soo),한군희(Han Kun-Hee) 한국산학기술학회 2009 한국산학기술학회 학술대회 Vol.- No.-

최근 개인정보유출사건으로 프라이버시에 대한 관심이 급증하면서, 데이터베이스의 내용을 암호화할 필요성이 요구된다. 초기에는 문서 전체의 복호화를 통해서만 검색이 가능하기 때문에 효율성이 떨어져 암호화기법이 거의 사용되지 않았다. 최근에는 복호화 하지 않고 암호화된 데이터로부터 특정 키워드를 포함하는 정보를 효율적으로 검색하고자 하는 연구가 시작되었다. Song의 연구를 시작으로 점차 효율적인 검색 기법이 제안되어졌다. 본 논문에서는 데이터베이스내의 암호화된 데이터를 검색하는 기법에 대한 설계 및 구현하고, 그에 따른 정확도 및 오류율을 분석한다.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

온풍 조건에서 수분 탈락 정도에 따른 피부 분류 및 개선 방안에 대한 연구

권오선 ( Oh Sun Kwon ),강현종 ( Hyun Jong Kang ),한승민 ( Seung Min Han ),윤지선 ( Ji S Eon Yoon ),조웅희 ( Woong Hee Cho ),오주영 ( Joo Young Oh ),임준만 ( Jun Man Lim ),송영숙 ( Young Sook Song ),박선규 ( Sun Gyoo Park ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.2

피부를 탄력있고 부드럽게 하는 역할은 각질층에 존재하는 수분량에 의해 좌우된다. 피부 수분량은 냉온풍, 건조환경 등 다양한 환경 변화에 의해 영향을 받음이 알려져 있으나, 개인 피부 차이에 따른 피부수분량 변화와 회복 정도에 대해서는 많은 연구가 이루어 지지 않은 실정이다. 본 연구에서는 온풍 조건하에서 피시험자들의 피부 수분 탈락 및 회복 정도를 비교 평가하여 새로운 피부 타입을 제시하고, 온풍 조건에서 저하되는 피부 수분량을 개선 시켜주는 효능 물질을 개발하고자 하였다. 온풍 환경 조성을 위해, 건강한 피험자(남: 10 명, 여: 39 명, 25 세 - 63 세)의 전완부에 온풍(30 cm, 40 ℃, 6 m/s)을 30 min 간 피부에 노출시켜, 피부 수분량의 변화를 평가하였다. 26명(남: 4 명, 여: 22 명, 평균 연령: 42.7 ± 9.4)이 온풍 노출전에 비하여 온풍 노출 후 수분량이 유의하게 감소하며, 노출 후 30 min이 지나도 회복이 되지 않았다. 온풍노출 후 수분량이 떨어지는 피험자(여: 10 명)를 대상으로 보수력이 높은 크림을 3 주간 전완부에 적용한 이후 동일 온풍 조건하에서 피부 수분량을 측정한 결과, 노출 30 min 후 피부 수분량이 온풍 노출 전 수준으로 회복됨을 확인하였다. 본 연구를 통하여 피부는 건조 조건에서 쉽게 수분을 잃어버리는 피부(탈수형 피부)가 존재하는 것을 확인하였다. 이는 앞으로 화장품 개발을 보습 기능뿐만 아니라, 이러한 환경변화에 따른 피부수분이 쉽게 빠져나가는 피부(탈수형 피부)의 특성에 맞는 제품의 효능 개발이 필요함을 보여준다. Elasticity and softness of the skin depend on the level of moisture present in the stratum corneum, which is known to be affected by various environmental changes, such as cold and hot winds and dry environments. However, not many studies have been conducted on changes in skin moisture and the degree of recovery due to individual skin differences. In the present study, we aimed to investigate the effect of warm air heating on skin hydration levels and develop moisturizing formulas to improve lowered skin hydration levels. In order to deliver warm air heating condition, heating dryer (40 ℃, 6 m/s, 30 cm apart from forearm) was applied into inner forearm of healthy subjects (male: 10, female: 39, age: 25 - 63) Among 49 subjects, 26 subjects showed significantly lowered skin hydration levels until 30 min after warm air heating exposure (lowered group). In addition, moisturizing cream with high water holding capacity was applied to forearm of 10 subjects in lowered group for 3 weeks and skin hydration levels after warm air heating were significantly improved at the levels of before application of warm air heating. From this study, we found out that there is a skin type that skin hydration levels are significantly decreased under warm air heating condition (dehydrated skin) and this dehydrated skin can be improved by moisturizing formulas with high water holding capacity.

Lactobacillus pentosus 발효에 의한 담쟁이덩굴 줄기 추출물의 항산화 및 세포보호 효과

박소현 ( So Hyun Park ),성준섭 ( Joon Seob Seong ),이건수 ( Keon Soo Lee ),박영민 ( Young Min Park ),현송화 ( Song Hua Xuan ),차미연 ( Mi Yeon Cha ),강희철 ( Hee Cheol Kang ),박수남 ( Soo Nam Park ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

본 연구에서는 담쟁이덩굴 줄기 70% 에탄올 추출물과 발효균주 Lactobacillus pentosus를 이용하여 발효시킨 담쟁이덩굴 줄기 발효추출물에 대하여 항산화 및 세포보호 효과를 측정하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH)를 이용한 자유라디칼 소거 활성(FSC₅₀)은 담쟁이덩굴 줄기 추출물 및 발효추출물이 각각 42.3 및 34.5 μ g/mL로 발효 후의 라디칼 소거활성이 약 18.4% 더 높게 나타났다. Lumiol-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂계에서의 총 항산화능(OSC₅₀) 평가에서도 담쟁이덩굴 줄기 추출물과 발효추출물은 각각 2.6 및 2.5 μ g/mL로 발효 후가 약 4.2% 정도 더 높은 총 항산화능을 나타냈다. ¹O₂로 유도된 적혈구 세포손상에 있어서 추출물 및 발효추출물의 세포 보호 효과(τ₅₀)는 50 μ g/mL에서 각각 126.4 및 173.0 min을 나타내어 발효 후 세포 보호 효과가 약 34.0% 더 높게 나타났다. 발효추출물은 지용성 항산화제로 알려진 (+)-α-tocopherol (43.4 min)보다도 3.9배 높은 세포 보호 활성을 보여주었다. 사람 섬유아세포인 Hs68을 대상으로 elastase 저해 활성을 조사하였다. Elastase 저해 활성(IC₅₀)은 담쟁이덩굴 줄기 추출물과 발효추출물에서 각각 873.6 및 687.8 μ g/mL로 발효 후에 elastase 저해 활성이 약 21.3% 더 높은 것으로 나타났다. 이상의 결과들은 담쟁이덩굴 줄기 발효추출물이 항산화 작용과 더불어 주름개선 효과를 가지는 천연 화장품 소재로써 응용 가능성이 있음을 시사한다. In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities (FSC₅₀) of non-fermented and fermented extracts were 42.3 and 34.5 μg/mL, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) in Fe³⁺-EDTA/H₂O₂ system of non-fermented and fermented extracts were 2.6 and 2.5 μg/mL, respectively. The activity after fermentation was approximately 4.2% higher. In the ¹O₂-induced cellular damage of erythrocytes, the cellular protective effects (τ₅₀) of non-fermented and fermented extracts were 126.4 and 173.0 min at 50 μg/mL, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than (+)-α-tocopherol (τ₅₀ = 43.4 min), known as a lipophilic antioxidant at 50 μg/mL. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities (IC₅₀) of non-fermented and fermented extracts were 873.6 and 687.8 μg/mL, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.