프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

기계적 자극을 이용한 인체 중간엽줄기세포로부터 유사-섬유모세포로의 분화 유도

최경민 ( Kyung Min Choi ),서영권 ( Young Kwon Seo ),윤희훈 ( Hee Hoon Yoon ),권순용 ( Soon Yong Kwon ),이화성 ( Hwa Sung Lee ),박용순 ( Yong Soon Park ),손영숙 ( Young Sook Son ),송계용 ( Kye Yong Song ),김영진 ( Young Jin Kim 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4

Recently, in vitro reconstruction of anterior cruciate ligament(ACL) has been tried using tissue engineering technique, especially mesenchymal stem cell(MSC) is considered as a good cell source. In this study, we tried to find the effect of mechanical stimulus on the differentiation of bone marrow-derived MSC into ACL fibroblastlike cell using a cell training bioreactor imposing cyclic mechanical tension whose parameters were 120 min/day, 1 Hz, and 5% strain. After 2 weeks of culture, MSCs under cyclic tension showed more regularly oriented alignment and lower expression of CD73 and CD105 than control by microscopy and FACS analysis. In transcriptional level, type I collagen, type II collagen, fibronectin, elastin, α-smooth muscle actin mRNA expressions of MSCs under cyclic tension were higher than control and similar to native ACL. In conclusion, it is thought that mechanical cyclic tension induced the loss of stemness of MSC, therefore improved the differentiation into ACL fibroblast-like cell.

Effects of Mechanical Stimulation on the Proliferation of Bone Marrow-derived Human Mesenchymal Stem Cells

Choi, Kyung-Min,Seo, Young-Kwon,Yoon, Hee-Hoon,Song, Kye-Yong,Kwon, Soon-Yong,Lee, Hwa-Sung,Park, Jung-Keug Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.6

To support and enhance the in vitro growth and activity of mesenchymal stem cells (MSCs), the cell culture medium may be supplemented with various proteins and factors to mimic the physiological environment in which the cells optimally proliferate and differentiate. In this study, the effects of mechanical factors on cellular metabolic responses were investigated experimentally using a bioreactor. The effects of various chemical factors, such as growth factors, cytokines, and hormones, were also investigated. Based on previous reports demonstrating the important roles of mechanical factors in the growth and activity of MSCs, we sought to evaluate the effects of mechanical stimuli on the proliferation of bone marrow-derived MSCs using a cell training bioreactor that imposed cyclic mechanical stretch, with parameters of 240 min/day, 0.03 Hz, and $5{\sim}15%$ strain. The application of cyclic stretch $(5{\sim}15%\;strain)$ to the MSCs enhanced their proliferation during the early stage(3 days), but not the late stage (14 days), of batch culture. Mechanical stretch did not increase the release of lactate dehydrogenase (LDH) from the MSCs during culture. Appropriate levels of mechanical stretch $(5{\sim}10%\;strain)$ increased collagen synthesis, but did not alter MSC stretch was able to serve as a potent positive modulator of MSC proliferation during the initial stages of culture.

온풍 조건에서 수분 탈락 정도에 따른 피부 분류 및 개선 방안에 대한 연구

권오선 ( Oh Sun Kwon ),강현종 ( Hyun Jong Kang ),한승민 ( Seung Min Han ),윤지선 ( Ji S Eon Yoon ),조웅희 ( Woong Hee Cho ),오주영 ( Joo Young Oh ),임준만 ( Jun Man Lim ),송영숙 ( Young Sook Song ),박선규 ( Sun Gyoo Park ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.2

피부를 탄력있고 부드럽게 하는 역할은 각질층에 존재하는 수분량에 의해 좌우된다. 피부 수분량은 냉온풍, 건조환경 등 다양한 환경 변화에 의해 영향을 받음이 알려져 있으나, 개인 피부 차이에 따른 피부수분량 변화와 회복 정도에 대해서는 많은 연구가 이루어 지지 않은 실정이다. 본 연구에서는 온풍 조건하에서 피시험자들의 피부 수분 탈락 및 회복 정도를 비교 평가하여 새로운 피부 타입을 제시하고, 온풍 조건에서 저하되는 피부 수분량을 개선 시켜주는 효능 물질을 개발하고자 하였다. 온풍 환경 조성을 위해, 건강한 피험자(남: 10 명, 여: 39 명, 25 세 - 63 세)의 전완부에 온풍(30 cm, 40 ℃, 6 m/s)을 30 min 간 피부에 노출시켜, 피부 수분량의 변화를 평가하였다. 26명(남: 4 명, 여: 22 명, 평균 연령: 42.7 ± 9.4)이 온풍 노출전에 비하여 온풍 노출 후 수분량이 유의하게 감소하며, 노출 후 30 min이 지나도 회복이 되지 않았다. 온풍노출 후 수분량이 떨어지는 피험자(여: 10 명)를 대상으로 보수력이 높은 크림을 3 주간 전완부에 적용한 이후 동일 온풍 조건하에서 피부 수분량을 측정한 결과, 노출 30 min 후 피부 수분량이 온풍 노출 전 수준으로 회복됨을 확인하였다. 본 연구를 통하여 피부는 건조 조건에서 쉽게 수분을 잃어버리는 피부(탈수형 피부)가 존재하는 것을 확인하였다. 이는 앞으로 화장품 개발을 보습 기능뿐만 아니라, 이러한 환경변화에 따른 피부수분이 쉽게 빠져나가는 피부(탈수형 피부)의 특성에 맞는 제품의 효능 개발이 필요함을 보여준다. Elasticity and softness of the skin depend on the level of moisture present in the stratum corneum, which is known to be affected by various environmental changes, such as cold and hot winds and dry environments. However, not many studies have been conducted on changes in skin moisture and the degree of recovery due to individual skin differences. In the present study, we aimed to investigate the effect of warm air heating on skin hydration levels and develop moisturizing formulas to improve lowered skin hydration levels. In order to deliver warm air heating condition, heating dryer (40 ℃, 6 m/s, 30 cm apart from forearm) was applied into inner forearm of healthy subjects (male: 10, female: 39, age: 25 - 63) Among 49 subjects, 26 subjects showed significantly lowered skin hydration levels until 30 min after warm air heating exposure (lowered group). In addition, moisturizing cream with high water holding capacity was applied to forearm of 10 subjects in lowered group for 3 weeks and skin hydration levels after warm air heating were significantly improved at the levels of before application of warm air heating. From this study, we found out that there is a skin type that skin hydration levels are significantly decreased under warm air heating condition (dehydrated skin) and this dehydrated skin can be improved by moisturizing formulas with high water holding capacity.

Improving the subcritical water extraction of flavonoids narirutin and hesperidin from Citrus unshiu peel by pulsed electric fields

Hui-Ju Kim,Mi-Ri Kwon,Hye-Ji Kang,Na-Yeon Kim,Hee-Jeong Hwang,Min-Jung Ko,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Citrus fruit is important source of flavonoids, mainly flavanones which are narirutin and hesperidin. Those citrus flavonoids have been found to have health-related properties including antioxidant, anticancer, and anti-inflammatory. The main purpose of this study was to verify that the extraction of narirutin and hesperidin from Citrus peel can be more effective by combining pulsed electric field (PEF) pre-treatment and subcritical water extraction (SWE). Citrus unshiu peels were treated with PEF under conditions of electric field strength (3 kV/cm) and times (1 and 2 min). Subsequent SWE was conducted by using a Dionex Accelerated Solvent Extractor (ASE, Model 350) at extraction temperature 170°C for 10 min. The total flavonoids content was measured by using the aluminum chloride colorimetric method and the antioxidant capacity was analyzed by the Ferric reducing antioxidant power (FRAP) assay using spectrophotometer. The concentrations of narirutin and hesperidin were increased as PEF pre-treatment time increased. The highest concentrations of narirutin and hesperidin were 13.41 mg narirutin/g dry citrus peel and 141.16 mg hesperidin/g dry citrus peel at PEF pre-treatment condition of 3 kV/cm and 2 min. The total flavonoids contents of the extracts increased 105.2% and 123.1% for citrus peel PEF treated at 1 and 2 min, respectively. In addition, compared to the untreated sample, PEF pre-treatments of 1 and 2 min increased the antioxidant capacity of the extracts 109.2% and 160.8%, respectively. Therefore, the results demonstrate the potential of PEF pre-treatment to improve the SWE of flavonoids from citrus unshiu peel.

허혈성 심질환에 있어서 아데노신 급속 정맥투여후 관동맥압의 변화에 관한 연구

봉종대,오종용,배성한,신원용,김철현,이광희,최태명,현민수,김성구,권영주 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Background: Adenosine interacts with A1 receptors present on the extracellular surface of cardiac cells, activating K+channels in a fashion. It produces coronary vasodilatation and AV nodal block. The half-time is one to six second and has ultra-short action. In ischemic heart disease, fractional flow reserve(FFR) can be achieved safely with intravenous adenosine infusions at a rate of 150㎍/kg/min which cause maximal coronary hyperemia. Purpose: We investigated the patients who have had ischemic heart disease(n=8). After adenosine intravenous administration(150㎍/kg/min), the effect cause maximal hyperemia of coronary artery. During peak maximal hyperemia, we reported clinical findings, coronary hemodynamics and electrocardiogram findings. Methods: After diagnostic coronary angiography and left ventriculography, catheter was advanced into the ostium of coronary artery. We investigated coronary hemodynamics such as systolic coronary artery pressure, diastolic coronary artery pressure, mean coronary artery pressure and heart rate. To assess the use of adenosine for determination of fractional flow reserve, hemodynamics and electrocardiogram were measured at basal state and during peak maximal hyperemia. Results: Symptoms developed 1.5-2 minute after adenosine intravenous administration and each symptom disappeared 2-2.5 minute after adenosine administration. Two patients represented asymptomatic appearance and six patients complained of flushing, chest pain, palpitation and headache. In eight patients with ischemic heart disease, 1.5 minute after venous administration of 150㎍/kg/min of adenosine, coronary artery pressure decreased and heart rate increased significantly. Conduction abnormalities after administration of 150㎍/kg/min of adenosine were transient 2nd degree AV block 2 cases(25%). Conclusions: Adenosine is a potent ultra short-acting vasodilator. Although coronary artery pressure decreased and heart rate increased and mild cardiovascular symptoms occurred after continuous infusion of 150㎍/kg/min of adenosine, it is useful as an agent for determination of fractional flow reserve.

심해 상어 간유에 의한 지방성 폐렴 2례

권경배,권삼,고희신,최성민,김두권,신형종,강승완,김우택 東國大學校醫學硏究所 1994 東國醫學 Vol.2 No.-

외인성 지방성 폐렴은 1925년 Laughlen에 의하여 처음으로 기술되었으며, 우리나라에서도 소아에서 다수가 보고되었다. 이 지방성 폐렴은 지방성 물질을 흡인 또는 흡입함으로서 쉽게 발생하며 임상적으로 질식, 빈호흡, 호흡곤란, 세균감염의 합병 및 성장 장애 등을 특징으로 한다. 방사선학적 소견으로는 병변의 부위는 환자의 자세와 투약 형태에 따라 드물며 광유 흡인에 의한 경우 종속 위치에서 기름을 비점적 흡인한 경우에 좀 더 광범위하게 분포를 하게되며 가장 잘 침범되는 부위는 우측 폐 하엽의 내측 분절과 심폐 분절 등이고 중력 의존적(gravity-dependent) 침윤에서부터 무기폐에 이르기까지 다양하게 나타난다. 예후는 침범된 병변의 부위, 환자의 전신상태 및 감염의 합병에 따라 다양하며 지방성 폐렴에 대한 특별한 치료가 없기 때문에 예방이 최선의 방법이다. 저자들은 심해 상어 간유 복용후 발생한 지방성 폐렴 2례를 경험하였다. 증례 1은 5개월된 남아로서 입원 당시 흉벽 함몰, 빈호흡을 호소하였고, 흉부 X선 촬영상 양폐야의 침윤을 나타내었고, 약 10개월 후에 실시한 흉부 X선 추적검사에서 병변은 여전히 남아있었다. 증례 2는 15개월된 여아로서 입원당시 잦은 상기도 감염, 발열 및 위식도 역류에 의한 성장 장애를 나타내었고, 흉부 X선 촬영상 양폐야의 침윤을 보였고, 약 9개월 후에 실시한 흉부 X선 추적검사에서 병변은 많이 호전되었다. 저자들은 심해 상어 간유에 의한 지방성 폐렴 2례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Exogenous lipoid pneumonia was first described by Laughlen in 1925 and a few cases of the pneumonia were reported during childhood in our country. It can be easily developed by the aspiration of lipoid substance through the airway. It is characterized by choke, tachypnea, chest retraction, dyspnea, superimposed bacterial infection and growth retardation. Roentgenographically the appearance is very diversed from gravity-dependent infiltration to atelectasis. The prognosis is also diversed according to the degree of involved lision, the general condition of the patients, and the superimposed infections. There's no specific treatment of lipoid peumonia. Therefore, prevention is the most important treatment. We experienced the two cases of exogenous lipoid pneumonia occurred shortly after having several capsules of the deepsea-shark-liveroil(Squalene). In the first case the 5-month-old male infant suffered from chest retraction and tachynea and the patchy and streaky infiltration of the both lung fields on the chest X-ray was revealed at the admission time. About 10 months later the lesion was remained reentgenographically. In the second case the 15-month-old female patient suffered from frequent upper respiratory infections, mild to moderate fever, and failure to thrive probably due to gastroesphageal reflusx. The chest X-ray was similar to the first case, but the lesion is nearly clear about 9 months after the discharge. We report two cases of lipoid pneumonia with brief review of relevant literature.

서울시에서 산발적으로 분리된 Salmonella enterica Serotype Typhi 임상분리균주들의 분자역학적 분석

이희무,김철현,손장욱,김민자,박미선,이복권 대한감염학회 2000 감염 Vol.32 No.5

항원결합에서 항-Z-DNA 단클론항체 Z44의 재조합 중쇄 및 경쇄 가변영역의 역할

권명희,주민경,서정규,김형일 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

It has long been known that both heavy(VH) and light chain variable region(VL) of antibody are involved in antigen binding. Recently, reports for anti-DNA Abs such as Z22 and Hed-10 have revealed that VH chain is more important than VL in recoginizing antigen. However, it is not certain yet whether the VH chain alone of these specific anti-DNA antibodies has dominant role or such dominance of VH chain can be applicable to other anti-DNA Abs. To investigate this possibility in connection with the role and contribution of VL chain, experiments were performed using mAb Z44 which binds to Z-DNA but rocognizes different epitopes from Z22 which share 95% sequence homology. The recombinant proteins including Z44VH and Z44VL were produced by E.coli. After purification of the these antibodies, the affinity and specificity of recombinant Ab proteins to DNA antigens were tested. The results were as follows; Z44VH bound most strongly to Z-DNA and bound also to ssDNA, whereas Z44VL did not bind to B-DNA, ssDNA, and Z-DNA. In vitro association of Z44VH with Z44VL has increased the affinity to Z-DNA synergistically compared with Z44VVH alone. In the case of association of Z22VH with Z22VL affinity to Z-DNA has showed synergistic increment compared with Z22VH alone. However, the associations of Z44VH with Z22VL or Z22VH with Z44VL did not show any significant increments of affinity. From our findings that Z44VH alone could bind to Z-DNA or to ssDNA and that Z 44VL could not, it could be concluded that Z44VH has dominant role in recognizing the antigen. In addition, it may be suggested that Z44VL can increase the affinity of Z44VH and play some role in changing the specificity of Z44VH.

대장암에서 림프절 전이의 위험인자

홍동민,천봉권,장희경 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Backgroud: Colonic and rectal cancers recently increase in incidence and motality in Korea. Although a variety of therapeutic modality has been applied, the result of therapy for the patients having lymph node metastases is not good. Especially the patient can have lymph node metastases in spite of early colonic and rectal cancer, and high recurrence rate is expected if non-radical therapy without adjuvent modality is applied to these patient in follow-up period. Therefore it is aimed to identify the risk factor affecting lymph node metastasis and to evaluate the possibility of the nodal metastases preoperatively and postoperatively. Methods: This retrospective study of 463 patients having suffered lower anterior resection, Miles operation or colectomy for colonic or rectal adenocarcinoma. Invasiveness, size of the carcinoma, age, vascular invasion, lymphovascular invasion, perineural invasion , sex, and grade of the carcinoma was evaluated as the possible risk factors of lymph node metastases. Results: Resected colonic and rectal adenocarcinoma reveals regional lymph node metastases in 46.3% cases. The nodal metastasis is statistically correlated with increase in tumor stage, tumor grade, and presence of lymphovascular and perineural invasion, and older age (p<0.05). Conclusion: Invasiveness, tumor grade, age, presence of lymphovascular invasion and perineural invasion would be risk factors expecting the regional lymph node metastasis in the colonic and rectal cancers. Therapeutic modality should be determined especially in tumor stage more than T1 because regional lymph node metastasis is expected.