심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

한국 전통장류가 한우 반힘줄모양근(M. Semitendinosus)으로 제조한 건염햄의 육질 특성에 미치는 영향

성필남,조수현,강근호,김동훈,박범영,강선문,박경미,Seong, Pil-Nam,Cho, Soo-Hyun,Kang, Geun-Ho,Kim, Dong-Hoon,Park, Beom-Young,Kang, Sun-Moon,Park, Kyoung-Mi 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.6

This study was conducted to develop a processing method for dry-curing beef ham with Korean traditional sauces and to investigate the quality properties of the dry-cured beef hams. Semitendinosus muscle from Hanwoo was spread with sundried salt (C), sun-dried salt mixed with red pepper paste (T1), and sun-dried salt mixed with soybean paste (T2), and inserted into washed Hanwoo large intestine. It was then dried and fermented for 75 d. Moisture contents of the hams were 46-48%, without any significant differences. T1 showed the lowest pH among the three dry-cured hams, while water holding capacity (WHC) of T2 was found the lowest (p<0.05). In mechanical color determination, C showed higher CIE $a^*$ and CIE $b^*$ than T1 and T2 (p<0.05), however CIE $L^*$ did not show any significant difference. In texture, T1 demonstrated significantly lower springiness than C (p<0.05). The sensory properties of all dry-cured hams did not show any significant difference, but T1 indicated a higher taste, saltiness and overall acceptability than others, whereas C gathered the highest score in color and flavor. In conclusion, the dry-curing by combination of sun-dried salt mixed with red pepper sauce (T1) showed higher quality properties of the Hanwoo dry-cured ham. 우리나라 전통장류를 건염햄 제조에 접목한 건염햄의 제품을 개발하기 위해 염지과정에서 첨가한 고추장과 된장이 건염햄 품질에 미치는 영향을 조사하고자 실시하였다. 천일염(C), 천일염+고추장(T1), 천일염+된장(T2)을 한우의 반힘줄모양근(m.emitendinosus)의 표면에 골고루 발라주고 세척한 한우 대장에 충진한 후 75일 동안 건조 및 발효시켰다. 한우 반힘줄모양근 건염햄 품질을 조사한 결과, 건염햄 수분함량은 46.45-48.04% 수준으로 유의적인 차이는 없었다. T1은 모든 처리구 중에서 pH가 가장 낮았고 T2는 WHC가 가장 낮았다(p<0.05). 기계적 육색 측정에서 C가 $a^*$와 $b^*$가 전통장류 처리(T1, T2)보다 높았으나(p<0.05), $L^*$은 유의적인 차이가 없었다. 조직감에서 T1은 C보다 탄력성이 유의적으로 낮았다(p<0.05). 관능평가 결과 모든 처리구에서 유의적인 차이가 없었고 C는 제품색과 향미가 높은 점수를 받은데 반해, T1은 높은 맛, 짠맛, 그리고 전체적 기호도가 다른 처리구에 비해 높았다.

농촌계몽운동에 나타난 계몽주의 사조의 성격고찰 : 브나로드운동을 중시으로

강선보,고미숙 安岩敎育學會 1997 한국교육학연구 Vol.3 No.1

본 연구에서는 브나로드운동의 성격을 지닌 농촌계몽운동에 나타난 계몽주의 사조의 성격을 고찰해 보았다. 1930년대 가장 대중적인 지지를 얻은 농촌계몽운동은 귀농운동의 성격을 띠었는데, 이것은 러시아 계몽주의 운동, 즉 1870년대 브나로드운동의 영향을 받은 것이었다. 또한 러시아를 경유한 프랑스 후기의 계몽주의가 크게 유입되었음을 알 수 있었다. 그 이유는 구한말의 실력양성 운동의 와중에서 서구사상이 크게 도입되었던 이유는 있었겠지만 8할을 점유하고 있는 농민을 계몽하기 위해서는 농촌이나 농민에 대한 긍정적인 시각을 갖고 있었던 사상이 훨씬 더 설득력이 있었기 때문인 것으로 사료된다. 이러한 브나로드운동으로서의 농촌계몽운동은 농민이 아닌 학생이나 도시 지식층이 주체가 되는 계몽주의적 한계를 지닌다.

전국 단위 감시체계의 현황과 전망

강성규,안연순,지영구,김형옥,하미나,권호장,백남종,김성아,홍윤철,김재용,강대희,정혜선,하은희 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

PT조건하 V_2O_5에 의한 Alcohol의 산화

강선희,김미련,김선자,김현옥,노복자,이기화,이정숙 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

2, 3; 5,6-di-O-isopropylidene-α-D -mannofuranose(ManA) were oxidized to their cor-responding 2, 3;5,6-di-O-isoprolylidene-α-D-manno-1.4-lactone(ManB) using calcium chlorite as oxidant in a phase transfer catalysis system. But identified results were not obvious. V_2O_5 catalzed oxidation of primary and secondary alcohols to aldehydes and ketones respectively at alkali conditions under Solod-Liquid phase transfer conditions. This reaction affords good overall yields of products without formation of undersirable.

胃疾患의 食餌療法

강미경,김미숙,김선숙,김수향,양이선 효성여자대학교 가정대학 학도호국단 1987 家政大論集 Vol.6 No.-

The circumstance being surrounded with a rapidly changing society causes us to build up manystresses during our modern life. For this reason, there is increasing the occurrence of gastroentericdisorder in frequency. The dietary treatment of gastroenteric disorder requires to prepare the program necessary fora meal's amount quality and cookery with careful attention. In addition, it is essential for gastroentericpatients to fully supplement the needed nutritive substance depending on the degree of the trouble,by controling their mealtimes. Consequently, the successful dietary treatment entirely depends uponthe close cooperation between the patient involving his family and doctors and dietitians becausethe patient has to observe the standard of the programed dietary treatment. And it also is very impor-tant that the person who prepares table for these patients, attempts to improve their appetite, bysupplying foods suitable for their digestion and taste on the basis of the dietary treatment's standard. Several mental elements of gastroenteric patients have great effect upon the recovery of their di-sorder so that their family and nurses must lead these patients to stabilize their mind and to berestored to health as well as the adequate medical treatment.

5년간 특수건강진단기관 분석정도관리 결과 분석

강성규,양정선,이미영,박인정,정호근 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목 적 : 1980년대 후반부터 산업보건에서 혈중 및 요중 중금속농도 분석의 정확성 문제가 크게 부각되었다. 같은 시료를 가지고 분석기관마다 보이는 커다란 오차로 인해 직업병 진단 결과에 대한 신뢰성을 크게 감소시켰다. 이에, 노동부에서는 1992년부터 특수건강진단 기관에 대한 생체시료 분석정도관리 프로그램을 시작하였다. 본 연구는 1995년부터 시작된 생체시료 분석정도관리 5년간의 결과를 보고하고, 현재 생물학적모니터링으로 사용되는 항목과 빈도를 조사하고 이 항목의 정도관리 참여율을 비교하여 산업보건 관계 연구나 직업병 관리에 참고할 수 있도록 하기 위해 실시하였다. 방 법 : 생체시료 분석정도관리는 무기와 유기분석분야로 나누어 일년에 전후반기 2회 실시하고 있으며, 지정항목과 자율항목으로 구분하고 있다. 지정항목인 혈중 납과 요중 마뇨산은 분야별로 한 번에 3개 농도수준을 주어 2개 이상이 기준값의 ±15%내외의 범위에 들면 적합한 것으로 판정하고 있으며, 자율항목은 2개의 시료를 주어 2개 모두 기준실험실 평균값의 ±3SD 내외의 범위에 들면 적합한 것으로 판정하고 있다. 결 과 : 지정항목인 혈중 납과 요중 마뇨산 분석은 평균 적합률이 각각 89%, 90% 이었고, 대학기관, 비영리법인, 종합병원, 사업장 자체기관별로 차이는 없었으나 신규 기판의 적합률 70% 수준으로 상대적으로 낮았다. 자율항목의 적합률은 평균 50% 수준이었고, 저농도보다는 고농도의 적합률이 떨어졌다. 국내에서 가장 많이 분석되고 있는 항목은 요중 마뇨산과 혈중 납이었고, 혈중 및 요중 망간도 많이 분석되고 있었다. 요중 삼염화물, 페놀, 만델산, 카드윰, 메칠마뇨산, 크롬 등도 많이 분석되고 있는 항목이었다. 혈중 망간, 요중 수은, 요중 NMF는 정도관리에서 적합판정을 받은 기관의 숫자보다 실제 분석을 하고 있는 기관의 숫자가 많았다. 결 론 : 지난 5년간의 생체시료 분석정도관리 결과 우리나라 분석실험실의 분석능력은 향상되고 있으나, 자율항목에 대한 분석능력은 아직 미흡한 것으로 나타났다. 그리고, 아직 일부 일부 항목에 대해서는 외부 정도관리 없이 분석되고 있는 경우도 있었다. Objective : The accuracy of analytical results of blood and urine heavy metals came out to the main issue on occupational health from late eighties. The discrepancy of the results for same samples from different laboratories made the diagnosis for occupational diseases be unreliable. Therefore, a quality control program for analysis of samples taken from workplace had been introduced in Korea since 1992. This study aims to show the quality control program for analysis of blood and urine samples and its proficient rates from 1992 to 1999 and to know how they have been being used in occupational health. Methods The quality control program runs twice a year with mandatory items of blood lead and urine hippuric acid and voluntary items of blood cadmium and manganese and urine mandellic acid and methyl hippuric acid. Participant laboratories are receiving three levels for each items and two out of three samples have to be qualified for being a profi-cient laboratory for the item. The acceptable range of blood lead and urine hippuric acid is ± 15% and that of the others is within 3 SD (standard deviation) from the reference values. Results : The proficient rates of blood lead and urine hippuric acid was 89%, 90%, repectively, however those of the other voluntary items have been from 51% to 62%. The proficient rates of urine mercury and urine N-methylformamide (NMF), which are introduced since 1999, were very poor. Urine hippuric acid and blood lead were analyzed frequently for the purpose of biological monitoring conducting by special health examination organizations. Urine and blood manganese and urine metabolites of trichloroethylene, urine phenol, methylhippuric acid and cadmium were followed. Conclusions : In conclusion, the quality control program for biological monitoring has dramatically improve the ability of analysing blood and urine samples and eventually con-tributes to diagnose occupational diseases and to prevent occupational poisoning. However, some biological monitoring data, such as urine manganese, mercury and NMF, have been still reported from laboratories that were not accepted as a proficient laboratory.

의료 서비스에 대한 만족도 측정 도구의 개발 : focused on Patients and their families

강소영,이선미 한국의료QA학회 1996 한국의료질향상학회지 Vol.3 No.1

Background : In these days, the health care organizations have concerned about customer-centered care in order to empower the competitiveness on the health care markets. The departments working for quality management of the hospitals have used health care quality indicators in terms of medical areas as well as service areas of the hospitals. However, there were insufficient efforts to develop the credible measurement t seek the customers' needs, their expectations and their satisfaction levels related to health care services because various kinds of challenges were in the process of scale development to measure customers' satisfaction in health care. The purpose of this study was to develop the satisfaction scale to health care services in a Korea health care organization and to test its tool with validity and reliability. Method : The concept of this tool was acceptability that is one of the components of health care quality defined by Donabedian. Acceptability has the five dimensions of concept : Accessibility; Patient-Practitioner Relations; Amenities; Patient Preferences as to the effects of care; and Patient Preferences as to the costs of care. The Satisfaction Tool to Health Care Services was reviewed by expert panel with five researchers, including hospital managers and a professor related to quality management of the hospitals. As a result, the content validity index was 84 in the outpatient satisfaction tool. The inpatient satisfaction tool had 87 of the content validity indes. The Satisfaction Tools to Health Care Services finally consisted of 44 items for outpatients/their families and of 60 items for inpatients/their families. Study subjects of the construct validity test were 479 outpatients/their families and 561 inpatients/their families who visited or admitted at a University hospital from July 1, 1996 through August 10, 1996. The data were examined by Factor Analysis with SPSS. Result : The items of the Satisfaction tools for outpatients/their families were categorized by eleven factors with eigenvalue greater than 1.0 accounting for 64.2 percent of the variation in item scores. Also, the items of inpatient tool had eleven factors with eigenvalue greater than 1.0 accounting for 60.3 percent of the variation in item scores. The reliability of overall scale were .95 and .96 for the outpatients/their families satisfaction scores and the inpatient/their families satisfaction scores. The internal consistency reliability with eleven factors was ranged from .30 to .94 for inpatients/their families. The Satisfaction Tool with eleven factors for inpatients/their families had internal consistency reliability ranged from 053 to .89. Conclusion : The Satisfaction Tools to Health Care Services focused on outpatients/their families and inpatients/their families developed in this study had a high reliability and the strong evidence of content validity and construct validity based on quality concept. Therefore, this tool would be utilized as a credible quality indicator of health care services to assess the quality problems and to monitor to quality improvement activities in Korean Health Care Organizations.

초등학교 사회과에서 TV광고 분석활동이 합리적 소비의식에 미치는 효과

강운선,신선미 대구대학교 사범대학 부설 교육연구소 2005 敎育硏究 Vol.1 No.1

본 연구의 목적은 초등학교 사회과에서 TV광고 분석활동 수업이 아동의 합리적 소비의식에 미치는 효과를 검증하여 그 의의를 밝히는 것이다. 이를 위하여 아동과 TV광고와의 관계 및 소비자 교육에 관한 선행연구를 고찰하여 초등학교 아동용 TV광고 분석활동 수업안을 구안하고 이를 구미 형일초등학교 5학년 아동에게 적용하였다. 적용이후 설문지를 통해 실험집단과 전통 강의식 수업을 실시한 통제집단간의 합리적 소비의식 점수를 산출, 이를 비교하여 사회과 ‘소비’ 관련 수업에서 TV광고 분석활동의 효과를 검증하였다. 설문자료 분석을 통하여 TV광고 분석활동 수업이 합리적 소비의식 긍정적인 영향을 미치는 것으로 밝혀졌다. 이에 근거하여 초등학교 사회과 소비자 교육에서 TV광고 분석활동 수업방법은 합리적 소비의식 함양과 수업현장에서 학습동기 강화에 효과적임을 제안하였다. As subject matter that can be treated in social class, commercial advertising is one of sources connected with today's consumption problems. It is also an important factor consumers need to look very cautiously in their everyday life. This study is an experimental study that demonstrates the effects of reasonable consumption mind-set by carrying out questionnaire survey after making draft of class for TV ads analysis activity on elementary school students and applying classes of social studies. Following hypothesis was established for experimental study. <Study Hypothesis> Children group who received class of TV ads analysis activities have higher score of reasonable consumption mind-set than those who received traditional lecture type class. The subordinate factor of the research is the sense of the consumption and the independent factor is the teaching method. And the controlled factor is gender, the academic background of tthe parents, social and economic standing, the amount of watching TV in a day and an allowance per month,etc which have an influence on the learning effect. Sample group of experimental study was randomly selected from 2 classes of the 5th graders of Hyeongil Primary Schook, Gumi city. The experimental group received class of TV ads analysis activity and control group received traditional lecture type class. Reasonable consumption mind-sets scores were calculated between 2 groups, compared and verified to demonstrate effects of TV ads analysis activities. This study utilized multiple regression analysis to analyze relative effects of teaching methods after carrying out frequency analysis for population statistical features, and giving Cronbach α value for verification of confidence of measuring tools. The category variables like genderc, teaching method,etc are used as variable facors. For practical data processing, SPSS PC Ver 10.0 was used as a statistical tool. As a result, the study hypothesis of this study was adopted as class for TV ads analysis activities showed higher average score of reasonable consumption mind-set by 11.464 than traditional lecture type class(p< .01). The result of this study demonstrated that TV ads analysis activity would be useful than existing lecture type class as one of teaching strategy of social studies for improvement of reasonable consumption mind-set, and further verified that this activity might be worthy of being included in curriculum of consumers in social studies for reducing damage of children consumers due to false ads or deceptive ads and for managing a reasonable consumption life.

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