Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

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청각장애초등학생을 위한 음악교육프로그램 개발 연구

이드보라ㆍ남선화ㆍ정미라(Debora LeeㆍNam Sun WhaㆍJeong Mi ra) 한국특수교육학회 2016 특수교육학연구 Vol.50 No.4

3′‐Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis

Kang, Li‐,Jung,Kwon, Eun‐,Soo,Lee, Kwang Min,Cho, Chanmi,Lee, Jae‐,In,Ryu, Young Bae,Youm, Tae Hyun,Jeon, Jimin,Cho, Mi Ra,Jeong, Seon‐,Yong,Lee, Sang‐,Rae,Kim, Wook,Yang John Wiley and Sons Inc. 2018 British journal of pharmacology Vol.175 No.23

<P><B>Background and Purpose</B></P><P>3′‐Sialyllactose (3′‐SL) is a safe compound that is present in high levels in human milk. Although it has anti‐inflammatory properties and supports immune homeostasis, its effect on collagen‐induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3′‐SL on the progression of rheumatoid arthritis (RA) in <I>in vitro</I> and <I>in vivo</I> models.</P><P><B>Experimental Approach</B></P><P>The anti‐arthritic effect of 3′‐SL was analysed with fibroblast‐like synoviocytes <I>in vitro</I> and an <I>in vivo</I> mouse model of CIA. RT‐PCR, Western blotting and ELISA were performed to evaluate its effects <I>in vitro</I>. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin‐O and haematoxylin staining.</P><P><B>Key Results</B></P><P>3′‐SL markedly alleviated the severity of CIA in the mice by reducing paw swelling, clinical scores, incidence rate, serum levels of inflammatory cytokines and autoantibody production. Moreover, 3′‐SL reduced synovitis and pannus formation and suppressed cartilage destruction by blocking secretion of chemokines, pro‐inflammatory cytokines, https://en.wikipedia.org/wiki/Matrix_metalloproteinases and osteoclastogenesis <I>via</I> NF‐κB signalling. Notably, phosphorylation of p65, which is a key protein in the NF‐κB signalling pathway, was totally blocked by 3′‐SL in the RA models.</P><P><B>Conclusions and Implications</B></P><P>3′‐SL ameliorated pathogenesis of CIA by suppressing catabolic factor expression, proliferation of inflammatory immune cells and osteoclastogenesis. These effects were mediated <I>via</I> blockade of the NF‐κB signalling pathway. Therefore, 3′‐SL exerted prophylactic and therapeutic effects and could be a novel therapeutic agent for the treatment of RA.</P>

복부비만 여성 대상 걷기 운동 프로그램의 비만관련 지표 및 심폐체력 개선 효과

이미라,김완수 김천과학대학 2007 김천과학대학 논문집 Vol.33 No.-

The purpose of this study was to investigate the effects of a 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, on obesity-related indices (body weight, body mass index, waist circumference, waist-to-bip ratio, body fat percent, and total body fat) and aerobic fitness in Korean women with abdominal obesity. The subjects were 11 middle-aged women with abdominal obesity (waist circumference ≥80 cm). The intervention consisted of walking for 45-60 min per day at an intensity of 40(50) to 60(65)% of heart rate reserve(HRR) for 3 days per week Subjects of this study had decreases in obesity-related indices overall. Decreases in body weight, waist-to-hip ratio, body mass index, waist circumference, and total body fat were statistically significant. Aerobic fitness was increased after the intervention and this increase was close to statistical significance. A 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, could be an effective strategy to reduce obesity-related indices in middle-aged Korean women with abdominal obesity. Whether higher amount of exercise is required to promote significant improvement in aerobic fitness in this population needs to be investigated in well-controlled further studies.

복합레진 수복시 와동의 크기 및 수복 방법이 교두 굴곡에 미치는 영향

이미라,이인복,석창인,이상탁,엄정문 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.6

The aim of this study was to measure the cusp deflection during composite restoration for MOD cavity in premolar and to examine the influence of cavity dimension, C-factor and restoration method on the cusp deflection. Thirty extracted maxillary premolar were prepared to four different sizes of MOD cavity and divided into six groups. The width and depth of the cavity were as follows. Group 1; 1.5 × 1 ㎜, Group 2; 1.5 × 2 ㎜, Group 3; 3 × 1 ㎜, and Group 4-6; 3 × 2 ㎜ respectively. Group 1-4 were restored using bulk filling method with Z-250 composite. However, Group 5 was restored incrementally, and Group 6 was restored with an indirect resin inlay. The cusp deflection was recorded at the buccal and lingual cusp tips using LVDT probe for 10,000 seconds. The measured cusp deflections were compared between groups, and the relationship between the cube of the length of cavity wall/the cube of the thickness of cavity wall (L³ / T³), C-factor and cusp deflection or %flexure (100 × cuspal deflection / cavity width) was analyzed. The cusp deflection of Group 1-4 were 12.1 ㎛, 17.2 ㎛, 16.2 ㎛ and 26.4 ㎛ respectively. The C-factor was related to the %flexure rather than the cusp deflection. There was a strong positive correlationship between the L³ / T³ and the cusp deflection. The cusp deflection of Group 5 and 6 were 17.4 ㎛ and 17.9 ㎛ respectively, which are much lower value than that of Group 4.

초등학생용 영어 학습 CD-ROM TITEL유형과 학습자 변인에 따른 학습 효과 비교연구

이동한,김은주,조미라 부산교육대학교 교육대학원 1999 논문집 Vol.1 No.-

The purpose of this study is to investigate the effectiveness of CD-Rom for speaking and listening study in elementary English class. This study also examines various types of CD-Rom titles used in the elementary English education and their effectiveness on learning English speaking and listening for the students who have different variables. The result shows that students mostly prefer game types of CDs in English learning. Game types of CDs are effective for improving listening competnece. However, the result of the studies represents that the effectiveness of the CDs are different for individuals. Educational effects are more significant for students who have had experience for using CDs than that of students who do not have experience. Moreover, other student variables are significantly related to the effectiveness of using CD-Rom titles in elmentary English education. Thus, teachers are required to analyze what types of CD-Rom titles should be used in their lessons and to whom they use before they use CDs in their English class.

Oct4 단백질의 DNA - 결합 영역에 융합된 HIV - 1 integrase

이민전,서진욱,김미라,신차균 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1

Human immunodeficience virus type-1 (HIV-1) integrase (IN) mediates integration of viral DNA into the host cell genome which is an essential step during the life cycle of retroviruses. HIV-1 IN shows four enzymatic activities in vitro: non-specific DNA- binding, endonucleolytic (3'-processing), integration (strand transfer), and disintegration activities. Oct4 as a transcription factor recognizes and binds the specific sequences containing 5' -ATGCAAAT-3'. In order to study the specific binding and enzymatic activities of the integrase protein, we constructed the four different fusion proteins (OctIN1, OctIN2, INKOct1, INKOct1) using different combination of HIV-1 IN and Oct4 protein. The fusion proteins were overexpressed in Escherichia coli and purified by Ni-NTA and SP-sepharose columns. Analysis of the enzymatic activities showed that all the four fusion proteins has detectable endonucleolytic and integration activities, suggesting that the fusion proteins can be used to study derected integration and sequence specific DNA-binding of the integrase fusion protein.

중년여성의 운동 예측모형

이미라,소희영 충남대학교 간호과학연구소 2002 충남대 간호학술지 Vol.5 No.1

This study was conducted to test the hypothetical model designed to explain exercise behavior of middle-aged women. This hypothetical model was based on behavior-related theories and exercise-related literature. Exogenous variables were exercise habit and perceived subjective norm. Endogenous variables were exercise benefit, exercise barrier, exercise-related affect, exercise self-efficacy, exercise intention, and exercise. A convenience sample of 152 middle-aged women was studied. SAS PC Program and LISREL 8.12a program were used for descriptive statistics and linear structural relationship(LISREL) modeling. The results were as follows. 1)The overall fit of the hypothetical model was acceptable(χ2 34.86(p=0.0015), χ2/df 2.49, RMSEA 0.09, standardized RMR 0.05, GFI 0.95, AGFI 0.85, NFI 0.92, NNFI 0.87, CFI 0.95). 2)The results of statistical testing of hypotheses were as follows. (1)The varibles in this hypothetical model explained 95% of the variance of the exercise behavior. (2)Habit was the most important variable to explain exercise behavior, followed by self-efficacy, barrier, affect and benefit. Subjective norm and intention were not significant predictors of exercise behavior. Habit and self-efficacy had a significant direct effect on exercise behavior. Barrier and intention did not directly influence on exercise behavior. Habit, benefit, barrier and affect had a significant indirect effect on exercise behavior through other variables in this hypothetical model. Norm had no indirect effect on exercise behavior. (3)The direct path between habit and benefit, habit and barrier, habit and self-efficacy, norm and barrier, benefit and affect, barrier and self-efficacy, and affect and self-efficacy were significant. The indirect path between habit and affect, habit and self-efficacy, benefit and self-efficacy, benefit and intention were significant. In conclusion, this study verified that the hypothetical model constructed in this study could be used effectively as a reference for further structural modeling or interventional studies.