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이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2
Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.
Oct4 단백질의 DNA - 결합 영역에 융합된 HIV - 1 integrase
이민전,서진욱,김미라,신차균 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1
Human immunodeficience virus type-1 (HIV-1) integrase (IN) mediates integration of viral DNA into the host cell genome which is an essential step during the life cycle of retroviruses. HIV-1 IN shows four enzymatic activities in vitro: non-specific DNA- binding, endonucleolytic (3'-processing), integration (strand transfer), and disintegration activities. Oct4 as a transcription factor recognizes and binds the specific sequences containing 5' -ATGCAAAT-3'. In order to study the specific binding and enzymatic activities of the integrase protein, we constructed the four different fusion proteins (OctIN1, OctIN2, INKOct1, INKOct1) using different combination of HIV-1 IN and Oct4 protein. The fusion proteins were overexpressed in Escherichia coli and purified by Ni-NTA and SP-sepharose columns. Analysis of the enzymatic activities showed that all the four fusion proteins has detectable endonucleolytic and integration activities, suggesting that the fusion proteins can be used to study derected integration and sequence specific DNA-binding of the integrase fusion protein.
Mi-Ra Kim,Jung-Min Yon,Se-Ra Lee,In-Jeoung Baek,Yun-Bae Kim,Beom-Jun Lee,Sang-Yoon Nam,Young Won Yun 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.2
Ginseng has been extensively used around the world for several thousand years as a food or a drug. Constituents in ginseng are known to have several activities such as antioxidant, anti-tumor, anti-stress, and anti-fatigue activities. In this study, we investigated the effects of Korean ginseng on the antioxidant system of female reproductive organs in mice. We analyzed the mRNA expression patterns of glutathione peroxidase (GPx) family in the reproductive organs of mice exposed to Korean ginseng. Korean ginseng extracts (0, 20, 200, and 2,000 ㎎/㎏ body weight) were treated daily by gavage to 8-week-old female mice for 5 weeks. Total RNAs were extracted from ovary and uterus, and then RT-PCR analysis was performed with the specific primers of each GPx genes. The cytosolic GPx mRNA expression was similar to control group in ovaries, but it was slightly increased in the uterus at all the doses of Korean ginseng. Gastrointestinal GPx mRNA was not changed in ovary, but was slightly affected in uterus. On the contrary, The phospholipid hydroperoxide GPx mRNA was weakly increased in ovary and uterus, but it was not significant difference. These findings suggest that Korean ginseng induces the slight increase on the expression of GPx genes in uterus rather than ovary, although the values didn't show a significant difference.
Mi-Ra Kim,Jung-Min Yon,Se-Ra Lee,In-Jeoung Baek,Yun-Bae Kim,Beom-Jun Lee,Young Won Yun,Sang-Yoon Nam 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.2
Ginseng is one of the most common herbal medicines in the Orient which has been used for the treatment of disease due to its wide spectrum of medicinal effects, such as tonic, immunomodulatory, antioxidant, and anti-aging activities. However, there are only a few reports on the effects of Korean ginseng on the reproductive organs in animals and humans. To understand the antioxidative effect of Korean ginseng on the male reproductive system, we analyzed the mRNA expression patterns of glutathione peroxidase (GPx) family [cytosolic (GPx1), gastrointestinal (GPx2), and phospholipid hydroperoxide (GPx4) types] in the testes, epididymes, prostates, and seminal vesicles of mice exposed to Korean ginseng. Korean ginseng extracts (20, 200, and 2,000 mg/kg body weight) were treated to 7-weekold male mice for 9 weeks daily per oral. Total RNAs were extracted from testis, epididymis, prostates, and seminal vesicles, and then RT-PCR was performed with the specific primers of each GPx genes. In testis, all type of GPx mRNA expression was similar to that of control group. However, the gene expression of GPx2 was decreased in the epididymis exposed to 2,000 ㎎/㎏ of Korean ginseng, but it was no significant difference. The gene expression of GPx1 in prostates was tend to decrease slightly compare to control group, but those of GPx2 and GPx4 were weakly increased by Korean ginseng treatments. On the contrary, the gene expressions of GPx2 and GPx4 in seminal vesicles were decreased slightly compare to control group. These findings suggest that Korean ginseng may influence the antioxidant system of male reproductive organs in a differential manner.
Distribution and Characteristics of Avian Influenza Viruses from Wild Birds in Mongolia
Hyun Mi Kang,Ok Mi Jeong,Min Chul Kim,Ji Sun Kwon,Mi Ra Paek,Hye Ryoung Kim,Yong Joo Kim,Kang Seuk Choi,Batchuluun D,Erdene Ochir T,Sodnomdarjaa R,Jun Hun Kwon,Youn Jeong Lee 대한인수공통전염병학회 2008 창립총회 및 학술대회 초록집 Vol.2008 No.1