Polyphenols of Rubus coreanum Inhibit Catecholamine Secretion from the Perfused Adrenal Medulla of SHRs

Byung-Sik Yu,Duck-Mi Na,Mi-Young Kang,Dong-Yoon Lim 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.6

The present study was attempted to investigate whether polyphenolic compounds isolated from wine, which is brewed from Rubus coreanum Miquel (PCRC), may affect the release of catecholamines (CA) from the isolated perfused adrenal medulla of the spontaneously hypertensive rats (SHRs), and to establish its mechanism of action. PCRC (20∼180Ռg/ml) perfused into an adrenal vein for 90 min relatively dose-dependently inhibited the CA secretory responses to ACh (5.32 mM), high K⁺ (56 mM), DMPP (100ՌM) and McN-A-343 (100ՌM). PCRC itself did not affect basal CA secretion (data not shown). Also, in the presence of PCRC (60Ռg/ml), the CA secretory responses to veratridine (a selective Na⁺ channel activator (10ՌM), Bay-K-8644 (a L-type dihydropyridine Ca²⁺ channel activator, 10ՌM), and cyclopiazonic acid (a cytoplasmic Ca²⁺-ATPase inhibitor, 10ՌM) were significantly reduced, respectively. In the simultaneous presence of PCRC (60Ռg/ml) and L-NAME (an inhibitor of NO synthase, 30ՌM), the inhibitory responses of PCRC on the CA secretion evoked by ACh, high K⁺, DMPP, and Bay-K-8644 were considerably recovered to the extent of the corresponding control secretion compared with that of PCRC-treatment alone. The level of NO released from adrenal medulla after the treatment of PCRC (60Ռg/ml) was greatly elevated compared with the corresponding basal level. Taken together, these results demonstrate that PCRC inhibits the CA secretion from the isolated perfused adrenal medulla of the SHRs evoked by stimulation of cholinergic receptors as well as by direct membrane-depolarization. It seems that this inhibitory effect of PCRC is mediated by blocking the influx of calcium and sodium into the adrenal medullary chromaffin cells of the SHRs as well as by inhibition of Ca²⁺ release from the cytoplasmic calcium store at least partly through the increased NO production due to the activation of NO synthase.

류마티스 관절염 환자에게 스테로이드 유발성 골다공증의 관리 상태에 대한 분석

유민아,이지수 이화여자대학교 의과대학 2005 EMJ (Ewha medical journal) Vol.28 No.1

Objectives : To examine the management status of patients with rheumatoid arthritis (RA) taking glucocorticoids treated at a tertiary hospital rheumatology Practice. Methods : A retrospective chart review was performed on 114 RA patients who were noted to be taking glucocorticoids during the period of 2000.7 to 2003.7, and who had been followed up for at least one year period. Use of bone densitometry and use of prescription medication for osteoporosis management were assessed. Results : Thirty one % of patients underwent bone densitometry, and 34% of patients received prescription medication for osteoporosis management. Sixty-six percent of patents who underwent bone densitometry showed osteoporosis with T score of less than 2.5, Osteoporosis medication was prescribed most often in the prednisolone exposure group of at least 7.5㎎/d for at least 6 months. Factors associated with not receiving management for osteoporosis included male sex, young age, and premenopausal woman. Conclusion : The use of bone densitometry and prescription medication for osteoporosis were suboptimal. Interventions to improve detection and prevention of glucocorticoid induced osteoporosis are necessary.

어패럴 패턴구조에 관한 연구(3) : 각 부위별 벌어짐량을 중심으로 Focusing on broadening volume per each part

나미향,민유숙 청주대학교 학술연구소 2005 淸大學術論集 Vol.5 No.-

I have developed a human body into body surface shell in order to find out the characteristics when the dimensional shape of human body is developed on a plane. I have reviewed characteristics of pattern with broadened and folded surface of front, side, and back part shaped along with winding of a human body, I have come to the following result. 1. In light of a front section, there has been a change on a hip for a breast dart volume, upper breast dart volume, under breast No. 1 and 2 dart volume, and abdomen from the aspect of relation among breast volume and type, and breast inclination and structural of a hip. 2. In light of a side section, its pattern structure is classified into three types of folded type, broadened type, and collected type. A broadened type is for a big hip physical shape, folded type for small hip physical shape, and collected type for same gap between waist and hip or no such a gap. 3. In light of a back section, its pattern structure varies depending upon structural characteristics of bending degree; dart volume of rear shoulder was classified into three types of large, medium, and small. A structural pattern of dart volume of a hip on a back abdomen was classified into hip-protruding physical shape, common physical shape, small hip physical shape, and its shaping characteristics were observed in the presence of a type collected to three types, broadened type, and folded type.

서울·경기 일부지역 대학생의 Take-out Food 이용실태

유성은,한영실,주나미 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.5

This study was designed to identify the teke-out food usage of university students in Seoul and Gyeonggi-do. Questionnaires were used to analyze these habits among 500 respondents. 1. The Frequency of Take-Out Food use: the greatest proportion of students useds take-out foods once or twice a week (33.8%). 2. The reasons for Take-Out Food use: fastness was the major reason (47.8%). 3. The day of the week for Take-Out Food use: the majority of the students used Take-Out foods regardless of the day (54.4%). 4. The time of day for Take-Out Food use: 6~8 p.m. for 31.6%. 5. The cost spent in each meal: 51.6% spent 3,000 to under 5,000 won. 6. The menus of Take-Out Food used: coffee was the most popular of all the take-out foods (43.6%). 7. Points considered when buying Take-Out Food: the majority of the college students thought tastes and quality of the foods were the most important considerations (51.0%). 8. The source of information about Take-Out Food: 43.4% of the students obtained information from their friends.

어패럴 패턴구조에 관한 연구(2) : 앞ㆍ옆ㆍ뒤의 차를 중심으로 Focusing on gap between front,side, and back

나미향,민유숙 청주대학교 학술연구소 2004 淸大學術論集 Vol.4 No.-

I have manufactured body surface shell using a plastered body shape taken from a real body in order to identify characteristics of body parts based upon the basic torso pattern. After developed body surface shell, I have come to this result by analyzing the result developed into 3physical shape characteristics of front,side, and back. 1. Development of body surface shell As a result of developing body surface shell to find out pattern structure, front board in all experiences formed a breast part space due to breast protrusion centered by B.P., in particular, there was a great amount of space on the sectional side dart volume and down the breast line including down the front lining. On a back, a waist dart was form on perpendicular with arm-hole dart on protrusion part of the scapula (shoulder blade) and there was a dart volume formed on interscye too. 2. Ration and difference between front, back, and side Each circumference of the front, back, and side showed significance in the field of breast, under breast, waist, abdomen, and hip. In addition, the gap between front and back showed significance in the filed of under breast and a hip. Accordingly, when designing tight fit clothing, the distribution of front, back and side should be configured differently per section. Its ratio showed outstandingly differently. 3. Gap between front and back of the pattern The value of body surface development diagram showed significance in four parts other than hip in light of front and back difference. Accordingly, a hip has no gap between front and back, upper breast girth showed bigger shaper in back than front, but front showed bigger value in breast circumference, under breast girth, waist girth, and abdomen circumference.

중금속 이온 존재 하에서의 아스코르브산 자동산화반응에 대한 단백질의 영향

김미옥,유리나 울산대학교 2002 생활과학논문집 Vol.4 No.1

Effects of superoxide dismutase(SOD), catalase, and such other proteins as bovine serum albumin(BSA), ovalbumin, lysozyme, and γ-globulin on the autoxidation rates of L-ascorbic acid(AsA) in the presence of heavy metal ions in aqueous solution and buffer solution were studied. AsA was dissolved in a ultra-refined water and 0.07 M potassium phosphate buffer (pH 7.4, treated Chelex-100) at a concentration of 50μM and 5 μM Fe(Ⅲ) or 0.1 μM Cu(Ⅱ) was added, and a oxygen gas was bubbled through the solution at a flow rate of 200 ml/min at 35℃. The amount of remaining AsA in the reaction mixture was determined by using a UV spectrophotometer(at 265 nm). It was confirmed that the ratio of remaining AsA was significantly larger in the presence of SOD, catalase, BSA, ovalbumin, lysozyme, and γ-globulin than in the absence of proteins. It was suggested that the non-enzymatic effects of SOD, catalase and some other proteins might be involves in the stabilization of AsA.

시판되는 생수 내 무기물 함량에 관한 연구

소유려,백병주,김재곤,양연미,김하나 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

생수 내에는 많은 미네랄이 포함하고 있으며, 이 중 칼슘, 나트륨, 칼륨, 마그네슘, 불소는 생수통에 반드시 표기해야 할 무기물이다. 칼슘, 마그네슘, 불소와 같은 무기물은 치아형성에 관여하며, 적절하게 섭취시 치아우식증을 예방할 수 있다. 현행 먹는 샘물 수질기준에 따르면 무해무기물질인 칼슘과 마그네슘에 대한 기준치는 없으며, 유해무기물질인 불소와 같은 무기물은 2 mgF/L 이하로 규정하고 있다. 본 연구는 국내에서 판매되고 있는 생수 15종을 대상으로 칼슘, 마그네슘, 불소의 농도를 측정하였고, 생수 내 무기물 함량의 표기 여부 및 무기물 농도를 비교, 검토하여 다음과 같은 결론을 얻었다. 1. 15개의 생수 중 1개의 생수를 제외하고 모두 칼슘함량을 표기하였다. 평균 칼슘농도는 34.68±31.84 mg/L, 최대 12.891±1.85 mg/L, 최소 2.0±0.02 mg/L이었다. 2. 15개의 생수 중 2개의 생수를 제외하고 모두 마그네슘 함량을 표기하였다. 평균 마그네슘 농도는 9.22±11.06 mg/L, 최대 30.43±0.75 mg/L, 최소 0.0 mg/L이었다. 3. 15개의 생수 중 4개의 생수를 제외하고 모두 불소 함량을 표기하였다. 평균 불소 농도는 0.25±0.33 mg/L, 최대 1.13±0.04 mg/L, 최소 0.01±0.03 mg/L이었다. 모두 생수는 현행 먹는 샘물 기준치인 2 mgF/L 이하에는 만족시켰다. Drinking water has lots of minerals, especially calcium, sodium, kalium, magnesium, and fluoride must be labelled on the bottle about their contents. Minerals like calcium, magnesium, and fluoride have influence to the tooth development. Appropriately taking some minerals, dental caries can be prevented somewhat degree. There is no guide line about innoxious minerals like calcium and magnesium, however, noxious mineral like fluoride, should be contained less than 2 mgF/L according to the current drinking water standard. Hereupon, it is necessary to recognize the concentration of fluoride in drinking water, so I studied 15 samples of domestic drinking water on sale about the concentration of calcium and magnesium, fluoride. The results obtained were as follows ; 1. 14 drinking waters in 15 samples showed various Ca concentration. The average Ca concentration is 34.68±31.84 mg/L. the highest is 128.91±1.85 mg/L and the lowest is 2.0±0.02 mg/L. 2. 13 drinking waters in 15 samples indicate the Mg concentration. The average concentration is 9.22±11.06 mg/L. the highest is 30.43±0.75 mg/L and the lowest is 0.0 mg/L. 3. 11 drinking waters in 15 samples indicate the F concentration. The average concentration is 0.25±0.33 mg/L, the highest is 1.13±0.04 mg/L and the lowest is 0.01±0.03 mg/L. All samples are satisfied the current drinking water standard, 2 mg F/L.

질산화 작용이 있는 Aeromonas hydrophila의 동정 및 특성

엄미나,장재철,유영희,지의상 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.6

폐수처리 중 생물학적 처리에 활용할 수 있는 질소분해 능력을 가진 미생물을 분리하여 동정하고자 경기도내 하천 6지점에서 채취한 시료로부터 50개 균주를 선별하였다. 형태학적, 생화학적 및 배양학적 실험결과 Bergey's mannual of systematic bacteriology의 색인을 통하여 Aeromonas hydrophila로 동정하였다. Aeromonas hydrophila(AH-1). (AH-3), (AH-4), (AH-6) 균이 질산화 능력이 우수하였다. 4개 균주 모두 amoxillin, ampicillin, cephalothin과 ticarcillin에 내성을 나타내었다. 본 실험에서 분리한 .Aeromonas hydrophila의 질산화의 최적조건은 균 농도 1.0×l0exp(6) cells/ml, 배양온도 37℃로 나타났다. For the purpose of the isolation of microorganisms which have the capability of nitrification, we isolated the microorganisms in 6 samples collected from the stream of Kyonggi area. 60 strains were isolated. The selected strain were identified as a Aeromonas hydrophila based on the data obtained from the morphological, biochemical and cultural characteristics defined experiments. Among them Aeromonas hydrophila (AH-1), (AH-3), (AH-4), (AH-6) showed the highest nitrification capability. All isolates were resistant to amoxillin, ampicillin, cephalothin and ticarcillin. Optimum culture conditions of isolates were 37℃ and 1×10exp(8) cells/ml for 4 hours in the nitrate medium.

Original Articles : Effect of aldosterone on the amplification of oncolytic vaccinia virus in human cancer Lines

( Hyun Ju Lee ),( Ja Sung Rho ),( Shao Ran Gui ),( Mi Kyung Kim ),( Yu Kyoung Lee ),( Yeon Sook Lee ),( Jeong Eun Kim ),( Eu Na Cho ),( Mong Cho ),( Tae Ho Hwang ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: JX-594 is an oncolytic virus derived from the Wyeth vaccinia strain that causes replication-dependent cytolysis and antitumor immunity. Starting with a cross-examination of clinical-trial samples from advanced hepatocellular carcinoma patients having high levels of aldosterone and virus amplification in JX-594 treatment, we investigated the association between virus amplification and aldosterone in human cancer cell lines. Methods: Cell proliferation was determined by a cell-counting-kit-based colorimetric assay, and vaccinia virus quantitation was performed by quantitative polymerase chain reaction (qPCR) and a viral plaque assay. Also, the intracellular pH was measured using a pH-sensitive dye. Results: Simultaneous treatment with JX-594 and aldosterone significantly increased viral replication in A2780, PC-3, and HepG2 cell lines, but not in U2OS cell lines. Furthermore, the aldosterone treatment time altered the JX-594 replication according to the cell line. The JX-594 replication peaked after 48 and 24 hours of treatment in PC-3 and HepG2 cells, respectively. qPCR showed that JX-594 entry across the plasma membrane was increased, however, the changes are not significant by the treatment. This was inhibited by treatment with spironolactone (an aldosterone-receptor inhibitor). JX-594 entry was significantly decreased by treatment with EIPA [5-(N-ethyl-N-isopropyl)amiloride; a Na+/H+-exchange inhibitor], but aldosterone significantly restored JX-594 entry even in the presence of EIPA. Intracellular alkalization was observed after aldosterone treatment but was acidified by EIPA treatment. Conclusions: Aldosterone stimulates JX-594 amplification via increased virus entry by affecting the H+ gradient. (Korean J Hepatol 2011;17:213-219)

[PE-0057] Genetic Diversity and Genome-wide Association Study of Korean Soybean Landraces(Glycine max(L.) Merr.)

Yu-Mi Choi(Yu-Mi Choi),XiaoHan Wang(XiaoHan Wang),Hye-Myeong Yoon(Hye-Myeong Yoon),On-Sook Hur(On-Sook Hur),Na-Young Ro(Na-Young Ro),Ho-Cheol Ko(Ho-Cheol Ko),Jung-Yoon Yi(Jung-Yoon Yi) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-