Serial values for hematologic and biochemical analysis after myocardial infarction in rats

Lee, Mi-Jin,Tae, Hyun-Jin,Li, Ying-Hua,Yu, Do-Hyeon,Han, In-Ae,Lee, Seok-Won,Ahn, Dong-Choon,Kim, In-Shik,Park, Jin-Ho The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Serial values for hematologic and biochemical analysis after myocardial infarction in rats

( Mi Jin Lee ),( Hyun Jin Tae ),( Ying Hua Li ),( Do Hyeon Yu ),( In Ae Han ),( Seok Won Lee ),( Dong Choon Ahn ),( In Shik Kim ),( Jin Ho Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction(MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hema-tologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and bioche-mical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery(LMDA) of the left coronary artery was ligated during the progression(day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group(n=24, thoracotomy without LMDA ligation) and the experimental(MI) group(n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell(RBCs), hemoglobin and hematocrit(HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, choles-terol, total creatine kinase(CK) and isoenzyme MB, and lactate dehydrogenase(LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

영남 지역 시첩(詩帖)제작의 한 국면 - 구암(龜巖) 이정(李禎) 가문의 『수서시(壽瑞詩)』를 중심으로 -

이미진 ( Lee Mi-jin ) 우리한문학회 2021 漢文學報 Vol.45 No.-

『壽瑞詩』는 李稵(1372~1495)의 장수를 기념하여 당시 右議政이었던 姜孟卿(1410~1461)이 지어준 祝壽詩및 후대의 次韻詩를 모아둔 詩帖이다. 이 시첩은 李稵의 후손인 龜巖李楨(1512~1571)이 제작을 주도하였는데, 여기에는 강맹경의 元韻이 지어진 作詩현장과 이후 여러 대에 걸쳐 지어진 次韻詩의 수습 과정이 그대로 남아있다는 점에서 눈여겨볼만하다. 『수서시』는 구암 한시를 모아둔 시집이 아니며, 문학적 완성도를 운운하기 위한 작품 선집도 아닌 만큼 이 시기 영남 지역을 중심으로 활동한 지식인들의 한시 授受장면을 엿볼 수 있는 흥미로운 자료라 할 수 있다. 구암 이정이 오랜 기간 영남 지역에서 지방관을 역임했다는 사실과 그 과정에서 차운시를 수습했던 사실에 주목하여 『수서시』의 제작 경위 및 차운시 현황을 살펴본 결과 차운시는, 강맹경 생존 당시, 구암의 지방관 재직 시, 그리고 구암 사후 수습되었다는 사실을 알 수 있었다. 구암은 영천군수(경북 영주)에 제수된 이후 경북 선산과 충북 청주를 거쳐 경북 경주와 전남 순천에 이르기까지 영남의 상하를 관통하면서 비교적 大邑의 수령을 지냈는데, 이 가운데 영주, 선산, 청주 지역에서 재직 시 요청하여 받은 차운시가 수록되어 있기 때문이다. 선대의 시문을 후손이 모아 제작했다는 점에서, 그리고 차운시의 대부분이 선조의 장수와 그의 후손들의 효행을 칭송하고 있다는 점에서 『수서시』는 명실상부한 구암 가문의 기록물의 성격을 지니고 있다고 할 수 있다. The 『Suseosi(壽瑞詩)』 is Sicheop(album of poems, 詩帖) collecting Chuksusi(祝壽詩) created by Gang Maenggyeong(姜孟卿, 1410-1461), the contemporary Uuijeong(state councilor), which expresses the longevity of Lee Ja(李稵, 1372-1495) from Sachoen(泗川) Lee’s family and Chaunsi(次韻詩) created by the next generation. This Sicheop, which was primarily created by Guam Leejeong (龜巖李楨, 1512-1571), a descendent of Lee Ja, shows the site in which Gang Maenggyeong’s Wonun(元韻) was created and the process in which Chaunsi which had been created across many generations was learned. The 『Suseosi』 is neither the collection of Guam’s Chinese poems nor an anthology with literary completion, but may be an interesting material with which it is possible to get a glimpse of the scenes in which intellectuals, who engaged in literary activities around Yeongnam province, exchange Chinese characters with each other. An analysis on the creation process of the 『Suseosi』 and the status of Chaunsi by focusing on the fact that Guam Lee Jeong served as a local official in Yeongnam province for a long time and learned Chaunsi in the process, shows that Chaunsi had been learned by his descendents, during the survival of Gang Maenggyeong and the period in which Guam served as an local official, and after the death of Guam. After being appointed as a Yeongcheon(Yeongju, Gyeongbuk) country headman, Guam held the post of a local governor in relatively bigger towns(大邑), by vertically penetrating Yeongnam, from Seonsan, Gyeongbuk and Cheongju, Chungbuk to Gyeongju, Gyeongbuk and Suncheon, Gyeongnam. The Chaunsi he asked to receive when he held office in Gyeongju, Seonsan and Cheongju was contained in the 『Suseosi』. The 『Suseosi』 is the de facto record of Guam's family, in that the descendants created it by collecting the ancestors’ poetic works and most of Chaunsi praise the ancestors’ longevity and his descendants’ filial conduct.

조선시대, 딸에서 며느리로 살아가기

이미진(Lee, Mi-jin) 대동한문학회 2017 大東漢文學 Vol.53 No.-

본고는 조선후기 知守齋 兪拓基(1691~1767)가 자신의 여러 딸과 며느리에게 남긴 祭文과 墓誌文을 대상으로, 한 집안의 가장이 지닌 여성 인식과 그 기록 속에 침투되어 있는 조선 사회의 이면을 읽어내는 것을 연구 목표로 한다. 유척기는 당대 영의정에 오를 정도로 사회적 명망이 높았던 관료문인이었지만, 슬하 4남 5녀중 아들 둘과 다섯 명의 딸을 잃고 세 명의 며 느리까지 잃었을 정도로 비통한 가정사를 지닌 가장이기도 했다. 유척기가 작성한 딸과 며느리에 대한 제문･묘지문을 살펴보면, 딸이기 보다 시댁에서의 며느리로서의 삶을 중심으로 서술되어 있음을 확인할 수 있다. 이는 당시 여성들이 딸이기 보다 며느리로서의 역할에 치중하였고 당시 사회가 여성들에게 이를 요구하고 기대했던 데에서 비롯된다. 특히 맏며느리 이씨 부 인의 경우, 유씨 집안 내 역할과 비중은 누구보다 막중했고 그중에서도 남아 출산의 임무는 시댁에서의 존재의 이유와 직접적으로 연동되어 있었는데, 이는 당시 며느리들이 남아를 출산할 때라야 실질적인 며느리로서 행세할 수 있었음을 의미한다. 유척기의 맏며느리 이씨 부인은 결국 남아를 출산하지 못한 상태에서 남편을 잃자 이듬해 따라 죽었고, 시아버지 유척기는 그녀의 죽음을 ‘烈婦’로 기록하였다. 이씨 부인은 남아를 출산하지 못한 상태에서 남편을 잃은 경우 從死하는 것이 진정한 효이자 烈의 실천이라 믿었던 것이다. 이렇듯 며느리의 남아 출산 임무는 조선 후기 사회가 요구했던 부인상 중 하나로써, 이 시 기는 혈연 중심의 가문의식이 고조된 당대에 그 집안의 맥을 잇고 가문 내 결집력을 공고화하기 위해 후사를 두는 것이 무엇보다 중요한 시대였기 때문이다. 그리하여 이 시기 상층부 여성들은 살아있는 아이의 엄마이기 보다 죽은 남편을 따라 죽는 것이 시댁의 家格을 제고하고 본인 사후에 누릴 수 있는 최고의 현달이라 판단하여 자살을 감행하였는데, 이는 당시 유교 지배 올로기 자장 안에서 남성 사대부 못지 않게 여성에게도 열의 윤리가 내면화되어 있었기에 가능한 행위라고 할 수 있다. 이에 본고에서는 조선후기 상층부 관료였던 유척기 집안의 딸과 며느리의 사례를 중심으로 당시 烈婦의 존재와 그 속에 담겨진 사회 분위기를 읽어내는 한편, 父系 중심의 조선후기 사회는 혼인을 기점으로 딸에서 며느리로의 변화를 요구하였고, 이에 여 성들은 딸이기보다 며느리로서의 정체성을 찾아가고 있던 시기였음을 확인하였다. This study aims to offer an insight on the awareness that the head of a household had toward women and the reverse side of Joseon Dynasty, based on funeral orations and epitaphs that Jisujae Yu Chukgi(1691~1767), in late Joseon Dynasty, had left to his daughters and daughters-in-law. Yu Chukgi was a then-prestigious government official and writer and became even a prime minister, but also a head of household with a grief-stricken family history who lost his two sons and five daughters, among three sons and five daughters, and three daughters-in-law. In the records about his daughters and daughters-in-law written by Yu Chukgi, he described largely about the life as a daughter-in-law rather than as a daughter. This is attributed to the fact that then-women largely played a role as a daughterin-law rather than as a daughter and then-society required women to do so. Yu Chukgi’s eldest daughter-in-law Ms. Lee’s role within the family was heavier than anyone else and among which, her duty to give birth to a son was directly related to her position within her husband’s home, which suggests that then-daughter-in-law could be a real daughter-in-law only if she gave birth to a son. Yu Chukgi’s eldest daughter-in-law Ms. Lee lost her husband without giving birth to a son and died following his husband next year, and her father in law Yu Chukgi called her death as a ‘virtuous woman’ in his records. Ms. Lee believed that if she lost her husband without giving birth to a son, she had to follow her husband and this was a real filial duty. Daughter-in-law’s duty to give birth to a son was one of the duties that then-Joseon Dynasty society required women to fulfill. Having a son to unify people within the family was more important than anything else in those days when blood-tied family was thought important. For this reason, women in upper classes died following their husbands who died, rather than becoming a mother of a child alive. They committed a suicide, thinking that it was the best act to enhance the value of their husband’s home, even after their death. This is attributable to the fact that then-women had internalized ethics as a virtuous woman as much as then-men as a nobleman (scholar-official) in the dominant ideology of Confucianism. Accordingly, this study aimed to understand the existence of virtuous women and the social atmosphere behind centering on the case of daughters and daughtersin- law of Yu Chukgi who became the prime minister in the late Joseon Dynasty and on the other hand, found that the late Joseon Dynasty that was centered on a paternal family required daughters, from their marriage on, to play a role as a daughter-in-law and accordingly women had to find their identity as a daughter-in-law rather than as a daughter.

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

간암 환자에 합병된 Citrobacter braakii 패혈증 1례 보고

이해미,배성하,이수남,이정우,신아영,류시영,이수연,윤문희,한선영,한남익,이영석,유진홍 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Citrobacter species is a gram-negative bacilli that can cause opportunistic infections in immunocompromised hosts. Citrobacter braakii refers to the genomospecies 6 of the Citrobacter freundii complex. There are no detailed studies on infections caused by this newly identified specific genetic species in Korea. We herein report a case of a patient with hepatocellular carcinoma who, after undergoing transcatheter arterial chemoembolization, developed biloma which later progressed to C.braakii sepsis and did not respond to treatment. To our knowledge, this is the first reported case in Korea on C. braakii infection resulting in septic shock in a patient with malignancy in Korea.

임상 검체에서 분리된 nontyphoid Salmonella에서의 integron의 빈도와 특징

이정은,박수진,김성한,김미나,이남용,이복권,이상오,김양수,우준희,최상호 대한감염학회 2009 감염과 화학요법 Vol.41 No.3

Background : Nontyphoid Salmonella (NTS) is a leading cause of human food-borne enteritis, It has been known that integron, a naturally occurring gene capture and expression element, Plays an important role in the development and dissemination of multidrug-resistance. In this study, we investigated the prevalences and molecular characteristics of integrons in NTS clinical strains. Materials and Methods : Between 1995-96 and 2000-03, a total 261 NTS clinical strains comprising 39 serotypes were collected from clinical specimens. All strains were serotyped, and the MICs of ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim were determined by agar dilution method. Integrons were detected by PCR amplification of integrase genes, and gene cassettes were determined by PCR and sequencing, Conjugation experiments were performed using E. coli J53 as a recipient. The clonal relationship was analyzed by pulsed-field gel electrophoresls (PFGE). Results : Of the 261 strains tested, class 1 integrons were present in 21 strains (8.0%). Class 2 and class 3 integrons were not found. The integron-positive rate was higher in S. Typhimurium (24.2% [8/33]) than in S. Enteritidis (2.0% [3/153]). Overall rates of antimicrobial resistance were higher in integron-positive strains. dhfr12-orfF-aadA2gene cassette was detected in 5 strains, aadA2in 4 strains, dhfr17-orfF-aadA5 in 2 strains, and addA1 in 1 strain. Ten integron-positive transconjugants were successfully selected. Among 8 integron-positive strains of S. Typhimurium, 7 had similar PFGE patterns. Conclusion: This study suggests that integrons are already playing a significant role in antimicrobial resistance in NTS. Continuous monitoring is needed to detect the emergence and spread of integron-mediated antimicrobial resistance.

일반 모델 덧씌우기 방식을 적용한 농촌지도공무원 역량모델 개발

김진모,주대진,이진화,길대환,주현미,이정은 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.2