패혈증을 동반한 Clostridium perfringens에 의한 간농양 1례

배인규,조용균,홍성수,김승규,조윤행,정두련,김미나,우준희,배직현,류지소 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.3

저자들은 아직 국내에 보고된 적이 없는 C. perfringens에 관한 간농양과 이에 동반된 전격성 패혈증으로 증상 발현 후 24시간 이내에 사망하였던 증례를 경험하였기에 보고하는 바이다. Clostridial infection usually occurred in association with trauma, malignancy, or intra-abdominal disease. Liver abscess by Clostridium perfringens is very rare and has not been reported in Korea .We experienced a case of liver abscess by C. perfringens in a 70-year-old man who was presented with fever. He developed a progressive jaundice and sepsis. Abdominal CT scan showed about 7×7 ㎝ sized liver abscess and gas formation within abscess. We underwent percutaneous drainage of a hepatic abscess and empirical antimicrobial therapy. The patient died less than 24 hours after presentation. C. perfnngens was identified from blood and aspirate of liver abscess.

간호사의 임상 실무 경험

서문자,손행미,강현숙,권성복,김주현,박영숙,이은희,임난영,조경숙,지성애 성인간호학회 2002 성인간호학회지 Vol.14 No.4

purpose: This study was conducted to describe qualitatively the entities of nurse's experiences in general hospitals and to suggest basic data guiding research on developing Standards of clinical nursing practice in Korea. Method: Fourteen nurses working at general hospitals with over 300 beds in Seoul were interviewed in-depth until saturation using tape-recorders and transcription. Result: The central theme of clinical nursing practice experienced by subjects was "being with clients" that means accepting client's personal character, solving client's needs and providing client-centered nursing. A also "being with clients" was felt to be the responsibility of nurses which was learned from their nursing schools. The nursing strategies performed in order to be with patients were proving skillful nursing techniques, accepting, educating, emotional support, advocating, and self-reflecting, the subjects experienced somewhat problematic affects such as difficulties in interpersonal relationship, work overload, negative image of nursing, deficit of self-confidence for nursing actions, poor working conditions, and unfair treatment. Nurses at the hospital practiced with pride when they felt that they were accepted by clients. Conclusion: Further research is needed to analysis problems in clinical practice and the comparison of nurses' experiences of clinical practice, with nurses' experiences in various settings.

Analysis of Error Type and Feedback Efficacy on Genre-Based Writing: Narrative Writing*

Mi-Haeng Cho(Mi-Haeng Cho) 한국영어어문교육학회 2022 영어어문교육 Vol.28 No.4

The current study aims to analyze EFL learners' error types and teachers’ feedback efficacy in English narrative writing. 60 participants divided into two proficiency groups by TOEIC scores were asked to write each of two narrative essays in class and correct each language-form error in their drafts based on two kinds of feedback (explicitly coded and uncoded). Errors in each group’s drafts and revisions were statistically analyzed based on the error types and feedback methods. The findings of the study are as follows: 1) Overall learners’ writing accuracy and fluency analyzed by tokens and error rate after revision show a significant improvement. 2) Detailed error analysis on narrative writing indicates that in the draft, the error types related to verbs (particularly past tense) are more frequent than others, but in the revisions, the correction activity using feedback shows a positive effect on error types relevant to verbs, one of treatable errors. 3) Students with better English proficiency, given more explicit “coded” feedback, can significantly reduce error rates in both drafts and revisions. This suggests teachers to consider troublesome error types as well as feedback methods when giving error feedback to EFL writers.

피드백 유형이 대학생의 영어 쓰기 수행에 미치는 영향

조미행(Cho, Mi-Haeng),이정원(Lee, Jeong-Won) 한국영어어문교육학회 2015 영어어문교육 Vol.21 No.2

The purpose of the current study is to investigate how EFL learners' error correction and writing performance is affected by the feedback types provided by the teacher. For this purpose, 38 college freshmen participated in this study. They were asked to take pre- and post-writing tests and write four essays. The teacher provided two different types of feedback (coded and uncoded) to learners’ drafts, focusing mainly on language forms, and the learners were required to submit the revised drafts based on the teacher’s feedback. The results of pre- and post-writing tests which were analyzed by the error rate showed a significant improvement in accuracy and fluency. This implies that recursive and constant writing practices with teachers’ feedback and learners’ self-correction had a positive effect on their English writing proficiency. Furthermore, coded feedback was more effective than the uncoded one for the improvement of Korean college students’ writing performance. It is hoped that the findings of the study will arouse a greater interest in exploring the most effective form of teacher feedback in EFL college writing classes.

발생에 따른 흰쥐의 위에서의 ghrelin mRNA의 발현 변화

조미행(Mi Haeng Cho),이상혁(Sang Hyuk Lee),김남희(Nam-Hee Kim),손현준(Hyun Joon Sohn),박정현(Jeonghyun Park),황승준(Seung Jun Hwang),김민선(Min-Seon Kim),박경한(Kyeong Han Park) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.3

Ghrelin은 growth hormone secretagogue로서 28개의 아미노산으로 구성된 펩티드호르몬이다. 성장호르몬 분비의 자극, 식욕의 조절, 위산 분비의 자극 등을 일으키는 것으로 알려진 ghrelin은 위, 뇌하수체, 작은창자 등에서 합성되며 특히 위에서 발생과정에 따라 그 분비 양상의 변화가 있음이 알려져 있으나 발생시기와 노화에 따른 ghrelin mRNA 발현에 대한 형태학적 연구는 없는 실정이다. 본 연구에서는 digoxigenin으로 표지한 oligonucleotide를 이용하여 발생시기별 Sprague-Dawley계 흰쥐에서 적출한 위 조직에 ghrelin mRNA에 대한 in situ hybridization을 시행하고 ghrelin mRNA 양성세포의 분포를 조사하였다. Ghrelin 양성세포는 임신 제18일 태아의 위에서는 관찰되지 않았으나 출생후 점진적으로 증가하여 6주에서 가장 많은 수의 양성세포가 관찰되었다. 이후에 양성세포의 숫자가 다시 감소하여 18월에 이르러 통계적으로 유의한 수준으로 감소하였다. Ghrelin 양성세포는 주로 위의 fundus 부위에 분포하였으며 발생 초기에는 위샘의 바닥부분에 소수가 분포하다가 발생이 진행됨에 따라 위샘의 윗부분까지 확산되어 분포하였다. 관찰된 양성세포는 대부분이 closed-type의 내분비세포였다. Ghrelin 양성세포는 발생 과정에 따라 그 분포와 숫자에 변화가 있었으며 사춘기에 가장 많은 숫자의 양성세포가 존재하였고 노화과정에 따라 다시 감소한 사실로 미루어 위에서의 ghrelin의 발현이 성장과 노화에 관련이 있는 것으로 추정된다. Ghrelin is a recently identified member of growth hormone secretagogue, which is synthesized as peptide of 28 amino acids in stomach, pituitary and intestines. Ghrelin controls food intake, and induces gastric acid secretion. It has been reported that synthesis and secretion of ghrelin changes according to age of animals. In this study, in situ hybridization technique with digoxigenin-labeled oligonucleotide probe is applied to elucidate the developmental change of ghrelin mRNA expression pattern in rat stomach. Ghrelin mRNA positive cells first appeared in stomach of 9 day rats, mainly in base of gastric glands. It showed the highest density and spread to neck area of the gastric gland in 6 week rats. Number of positive cells decreased significantly in 18 months. Most of ghrelin mRNA positive cells were found as closed-type endocrine cells in fundus area. These results suggest that ghrelin expression may be involved in development and ageing process.

흰쥐 뇌하수체에서의 NCX와 NCKX isoform mRNA의 발현에 관한 연구

조미행(Mi Haeng Cho),박경한(Kyeong Han Park) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.4

신경계통의 calcium homeostasis에 관여하는 exchanger로 NCX (Na+-Ca2+ exchanger)와 NCKX (K+-dependent Na+-Ca2+ exchanger)라는 두 종류의 단백질 집단이 존재한다. 세포 내 Na+과 Ca2+의 농도를 조절하며 calcium clearance 작용을 수행하는 NCX와 NCKX는 신경조직 등에서는 그 분포가 자세히 알려져 있으나 뇌하수체에서의 발현에 관한 형태학적 연구는 아직 보고된 바가 없다. 본 연구에서는 digoxigenin이 표지 된 NCX와 NCKX isoform의 riboprobe를 이용하여 흰쥐의 뇌하수체와 시상하부에 in situ hybridization을 시행한 후 각 isoform mRNA들의 발현 양상을 관찰하고 면역조직화학기법과의 이중 염색을 통해 그 분포를 조사하였다. 그 결과 뇌하수체의 먼쪽부분 (pars distalis)에서 NCX2 mRNA가 강하게 발현되었고, 신경부분 (pars nervosa)에서 NCX2와 NCKX2 mRNA의 반응이 관찰되었다. 그리고 pars nervosa와 신경로가 연결되어 있는 시상하부의 시각위핵에서도 NCX2와 NCKX2의 발현이 관찰되었다. 특히 NCX2의 경우 pars distalis의 성장호르몬 분비세포 중 68.2%에서 발현되었으며, 비내분비세포인 소포별세포(folliculostellate cell)에서는 전혀 발현되지 않았다. 이상의 결과는 뇌하수체 앞엽에서 NCX2가, 그리고 뒤엽에서 NCX2와 NCKX2가 내분비 작용 조절에 관여할 것임을 시사한다. Na+-Ca2+ exchanger plays a fundamental role in controlling the changes in intracellular concentration of Na+ and Ca2+ ions. Two different families of Na+-Ca2+ exchanger, NCX (K+-independent Na+-Ca2+ exchanger) and NCKX (K+-dependent Na+-Ca2+ exchanger), are known and each family includes several isoforms. But little is known about their expression in pituitary gland. In this study, in situ hybridization with digoxigeninlabeled riboprobe and double-labeled experiments with immunohistochemistry were applied to investigate the expression of NCX and NCKX mRNAs and their distribution in normal rat pituitary gland. NCX2 mRNA hybridization signals were expressed in pars distalis, while both NCX2 and NCKX2 mRNAs expression were strongly observed in pars nervosa. NCX2 and NCKX2 mRNA were also expressed in supraoptic nucleus of hypothalamus. In pars distalis, 68.2% of growth hormone secreting cells was colocalized with NCX2 mRNA, whereas NCX2 mRNA was not found in S100 positive folliculostellate cells. These results suggest that NCX2 in pars distalis and NCX2 and NCKX2 in pars nervosa appear to be involved in endocrine function of pituitary gland.

췌십이장절제술 환자의 휴식대사량 측정방법 비교 분석

김선형 ( Seon Hyeong Kim ),유희철 ( Hee Chul Yu ),조백환 ( Baik Hwan Cho ),채수완 ( Soo Wan Chae ),정미진 ( Mi Jin Jeong ),최영란 ( Yeong Ran Choi ),김행순 ( Haeng Sun Kim ),김주신 ( Ju Sin Kim ),김숙배 ( Sook Bae Kim ) 한국정맥경장영양학회 2012 한국정맥경장영양학회지 Vol.5 No.1

Purpose: An accurate determination of energy expenditure is needed to prevent postoperative complications and provide effective medical care for surgical patients. The aim of this study was to compare measured resting energy expenditure (REE) in patients undergoing pancreaticoduodenectomy (PD) using an indirect calorimetry (IC) with the help of five predictive equations. Methods: The subjects chosen for the study were 18 patients (12 males, 6 females) who underwent PD at Chonbuk National University Hospital between March 2010 and October 2011. REE was measured by IC (ICREE) and calculated with the following five predictive equations - (eqREE)-Harris-Benedict (HB), Mifflin St. Jeor (MI), American College of Chest Physician (AC), Ireton- Jones (IJ) and Cunningham (CU) equation - on postoperative day 3. Results: The mean measured REE by IC was 1,117.9 ±180.2, whereas the mean predicted REE by HB, MI, AC, IJ and CU equations were 1,241.3±156.1, 1,226±153.0, 1,401.7±188.0, 1,355.1±342.7, and 1,324.8±188.7 kcal/ day, respectively. The mean predicted REE by HB, MI, AC, IJ, and CU were found to be significantly different from the measured ICREE. The intraclass correlation coefficient ICC), which describes the degree of agreement with the ICREE, was significantly highest in CUeq REE (r= 0.679). The rate of accuracy of HBeq REE, MIeq REE, ACeq REE, IJeq REE, and CUeq REE for ICREE was 27.8%, 50.0%, 27.8%, 22.2%, and 23.5%, respectively, in individual subjects. Conclusion: The intraclass correlation coefficient (r= 0.124∼0.679) and accuracy rate (22.2∼50.0%) determined from the results of REE measured by IC and that calculated by five predictive equations were low in overall. The low accuracy rate of energy expenditure by equations, which apply uniform indicators, can be explained by ongoing metabolic changes of the patient. Thus, the use of IC is recommended to assess energy requirements in PD; when this method is not practical in clinical settings, nutrition intervention is needed through regular monitoring because of the low accuracy rate of the predictive methods and ICREE that can cause inappropriate energy management, leading to metabolic disorders. (JKSPEN 2013;5(1):24-30).

The Effects of Aromatherapy on Intensive Care Unit Patients' Stress and Sleep Quality: A Nonrandomised Controlled Trial

Cho, Eun Hee,Lee, Mi-Young,Hur, Myung-Haeng Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-

<P><B>Background</B></P><P> Stress has both physiological and psychological effects and can negatively impact patients' treatment and recovery. We examined whether the aromatherapy alleviated patients' stress and improved their sleep quality and provided data that can be utilized in clinical settings.</P><P><B> Methods</B></P><P> This was a nonrandomised controlled experimental study. Participants included lucid adult patients who were admitted to the intensive care unit and had spent more than two nights there. The experimental treatment required participants to engage in deep breathing with essential oils as part of the aromatherapy. The control group was instructed to go to sleep without receiving the lavender aroma oil.</P><P><B> Results</B></P><P> The experimental group and control group showed a significant difference in perceived stress (<I>F</I> = 60.11, <I>p</I> < .001), objective stress index (<I>F</I> = 25.65, <I>p</I> < .001), systolic blood pressure (<I>F</I> = 9.09, <I>p</I> < .001), diastolic blood pressure (<I>F</I> = 2.47, <I>p</I> = .046), heart rate (<I>F</I> = 5.71, <I>p</I> < .001), and sleep quality (<I>F</I> = 109.46, <I>p</I> < .001).</P><P><B> Conclusions</B></P><P> The results revealed that aromatherapy alleviated stress and improved sleep quality in intensive care unit patients after 2 days of the experimental treatment. These results demonstrate that aromatherapy affects stress and sleep quality, thus indicating its value in nursing interventions. This trial is registered with KCT0002344.</P>

Effects of Aromatherapy on the Anxiety, Vital Signs, and Sleep Quality of Percutaneous Coronary Intervention Patients in Intensive Care Units

Cho, Mi-Yeon,Min, Eun Sil,Hur, Myung-Haeng,Lee, Myeong Soo Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>The purpose of this study was to investigate the effects of aromatherapy on the anxiety, sleep, and blood pressure (BP) of percutaneous coronary intervention (PCI) patients in an intensive care unit (ICU). Fifty-six patients with PCI in ICU were evenly allocated to either the aromatherapy or conventional nursing care. Aromatherapy essential oils were blended with lavender, roman chamomile, and neroli with a 6 : 2 : 0.5 ratio. Participants received 10 times treatment before PCI, and the same essential oils were inhaled another 10 times after PCI. Outcome measures patients' state anxiety, sleeping quality, and BP. An aromatherapy group showed significantly low anxiety (<I>t</I> = 5.99, <I>P</I> < .001) and improving sleep quality (<I>t</I> = −3.65, <I>P</I> = .001) compared with conventional nursing intervention. The systolic BP of both groups did not show a significant difference by time or in a group-by-time interaction; however, a significant difference was observed between groups (<I>F</I> = 4.63, <I>P</I> = .036). The diastolic BP did not show any significant difference by time or by a group-by-time interaction; however, a significant difference was observed between groups (<I>F</I> = 6.93, <I>P</I> = .011). In conclusion, the aromatherapy effectively reduced the anxiety levels and increased the sleep quality of PCI patients admitted to the ICU. Aromatherapy may be used as an independent nursing intervention for reducing the anxiety levels and improving the sleep quality of PCI patients.</P>

F-70 Generation of lung cancer cell lines harboring EGFR T790M mutation using CRISPR/Cas9-mediated genome editing

( Mi-young Park ),( Min Hee Jung ),( Eun Young Eo ),( Seokjoong Kim ),( Sang Hoon Lee ),( Yeon Joo Lee ),( Jong Sun Park ),( Young Jae Cho ),( Jin Haeng Chung ),( Cheol Hyeon Kim ),( Ho Il Yoon ),( Ja 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.0

Tyrosine kinase inhibitors (TKIs) such as gefitinib and erlotinib are effective against lung adenocarcinomas harboring epidermal growth factor receptor (EGFR) mutations. However, cancer cells can develop resistance to these agents with prolonged ex-posure; in over 50% of cases, this is attributable to the EGFR T790M mutation. Moreover, additional resistance mutations can arise with the use of new drugs. Cancer cell lines with specific mutations can enable the study of resistance mechanisms. In this study, we introduced the EGFR T790M mutation into the PC9 human lung cancer cell line which has a deletion in exon 19 of the EGFR gene by the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)9-mediated genome editing. EGFR pyrosequencing revealed that PC9-EGFR T790M cell clones had a higher T790M muta-tion rate than PC9-G cells generated by long-term exposure to gefitinib; moreover, resistance in these clones was comparable to or higher than that in PC9-G cells. The clones were also highly sensitive to the 3rd-generation EGFR TKI (AZD9291), which is cytotoxic to lung cancer cells with EGFR T790M. The CRISPR/Cas9 programmable nuclease system can be used to generate various cancer cell lines with specific mutations that can facilitate studies on resistance mechanisms and drug efficacy.