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Visualization of Candidate Division OP3 Cocci in Limonene-Degrading Methanogenic Cultures
( Amelia Elena Rotaru ),( Regina Schauer ),( Christina Probian ),( Marc Mussmann ),( Jens Harder ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.4
Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonenedegrading community.