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Purification,Characterization and Cellular Localization of Klebsiella aerogenes UreG Protein
Lee, Mann Hyung 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.4
The K. aerogenes ureG gene product was previously shown to facilitate assembly of the urease metallocenter (Lee, M. H., Mulrooney, S. B., Renner, M. J., Markowicz, Y., and Hausinger, R. P. (1992) J. Bacteriol. 174, 4324-4330). UreG protein has now been purified and characterized. Although the protein is predicted to possess a putative NTP-binding P-loop motif, equilibrium dialysis studies showed negative results. Immunogold electron microscopic studies using polyclonal antibodies directed against UreG protein confirm that UreG is located in the cytoplasm as predicted in the DNA sequence.
Lee, Mann Hyung,Lim, Yu Mi,Sung, Jae Young The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.3
Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a major virulence factor and in a potent immunogen. In order to express the recombinant urease at a higher level, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a high copy-number vector. The recombinant H. pylori urease expressed in an E. coli strain that was grown in a rich medium supplemented with added nickel was purified to near homogeneity by using DEAE-Sepharose, Superdex HR200, and Mono-Q(FPLC) columns and the purified enzyme possessed the specific activity of 1255U/mg. Polyclonal antibodies raised against the purified recombinant H. pylori urease were shown to be very specific when subjected to Western blot analysis, in which crude extracts from the H. pylori ATCC strain and the recombinant E. coli strains expressing various bacterial ureases were examined for cross-reactivity.
Association of chronic viral hepatitis B with insulin resistance.
Lee, Jeong Gyu,Lee, Sangyeoup,Kim, Yun Jin,Cho, Byung Mann,Park, Joo Sung,Kim, Hyung Hoi,Cheong, Jaehun,Jeong, Dong Wook,Lee, Yu Hyun,Cho, Young Hye,Bae, Mi Jin,Choi, Eun Jung WJG Press 2012 WORLD JOURNAL OF GASTROENTEROLOGY Vol.18 No.42
<P>To investigate the relationship between chronic viral hepatitis B (CVHB) and insulin resistance (IR) in Korean adults.</P>
Lee, Mann Hyung,Lim, Yu Mi,Sung, Jae Young 생화학분자생물학회 1999 BMB Reports Vol.31 No.3
Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a major virulence factor and in a potent immunogen. In order to express the recombinant urease at a higher level, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a high copy-number vector. The recombinant H. pylori urease expressed in an E. coli strain that was grown in a rich medium supplemented with added nickel was purified to near homogeneity by using DEAE-Sepharose, Superdex HR200, and Mono-Q (FPLC) columns and the purified enzyme possessed the specific activity of 1255 U/㎎. Polyclonal antibodies raised against the purified recombinant H. pylori urease were shown to be very specific when subjected to Western blot analysis, in which crude extracts from the H. pylori ATCC strain and the recombinant E. coli strains expressing various bacterial ureases were examined for cross-reactivity.
한국산 고등군류의 성분연구(20) : Coprinus comatus와 Strobilomyces floccopus의 성분
이만형(Mann-Hyung Lee),김병각(Byong-Kak Kim) 한국생약학회 1980 생약학회지 Vol.11 No.1
In order to investigate constituents of Coprinus comatus (Fr.) S.F. Gray and Strobilomyces floccopus (Fr.) Karst., free and total amino acids of the two mushrooms were quantively analyzed by GLC and an amino acid analyzer. Fats were extracted from the mushrooms and saponified with alcoholic potassium hydroxide. The isolated fatty acids were methylated and subjected to column chromatography and GLC.