한국산 노루발풀의 유전적 변이와 집단구조

이인섭,문성기,허만규 慶星大學校 1997 論文集 Vol.18 No.2

Pyrola japonica (Pyrolaceae) is a perennial herbaceous species to Asia. This species reproduces both asexually by underground rhizomes and sexually by seeds. The level of genetic diversity of this species was surveyed using starch gel electrophoresis at putative six enzyme loci from 11 natural populations in Korea. Results from six loci indicated that genetic diversity was high and population divergence was found between genetic identity statistics and geographic distance between populations. Although the range of genetic diversity statistics among populations was unsually large, genetic drift and asexual reproduction did not appear to play major roles in structuring genetic variation. We concluded that the level of genetic diversity maintained within P. japonica populations and the level of population divergence was influenced by its status as forest and mode of gene flow.

Urapidil, Labetalol의 투여가 기관내 삽관시 심혈관계에 미치는 영향

신용섭,윤석화,손수창,이원형,이정은,황원재,김만수,김영주,김혜자,최세진 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

We have examined the comparative efficacy of small doses of intravenous urapidil and labetalol in blunting hemodynamic response to endotracheal intubation and surgical incision in 30 patients without cardiovascular diseases. After intravenous urapidil 0.2 mg/kg or labetalol 0.2 mg/kg anesthesia was induced with thiopental 5mg/kg. Endotracheal intubation was facilitated by vecuronium 0.15 mg/kg with priming principle and anesthesia was maintained with enflurane and nitrous oxide in oxygen. Systolic, diastolic and mean arterial pressure and heart rate were measured before administration of the drugs, 5 minute after administration, just prior to endotracheal intubation and 1, 3, 5, 10 minute after intubatin. Also the peak blood pressures and heart rate within 10 minutes after surgical incision were measured. Endotracheal intubation and surgical stimulation were associated with significant increases in blood pressures and heart rate in both urapidil and labetalol group. Comparison of the changes in systolic, diastolic, and mean artrial pressures and heart rate between urapidil and labetalol group showed no significant difference except peak systolic pressure after surgical incision. It is concluded that the pressor response to endotracheal intubation and surgical stimulation are not influenced significantly by urapidil 0.2 mg/kg or labetalol 0.2 mg/kg. However, urapidil and labetalol preloading may be similarly effective in the blunting of the increases in blood pressures with larger doses of the durgs during anesthetic induction.

조성음악 안에서의 Schoenberg의 대위법적인 기법 연구

한만섭,이진우 全南大學校 藝術硏究所 1995 藝術論集 Vol.1 No.-

Counterpoint is a traditional art, and Schoenberg, as a practitioner of this art, evolved out of tridition and maintained an essentially traditional outlook, as revealed in his many statements and in his works, which show the influence of Brahms, Wagner, and Mahler. His early works also reveal him as an essentially countrapuntal composer. Bach had the primary influence upon his contrapuntal idiom; from Wagner he acquired the concept of motivic and thematic combination, and from both Wagner and Mahler a linear concept fundamentally. Schoenberg represented the culmination of a ployphonic and contrapuntal reactivation which began with Wagner, and which coincided with a breakdown of traditional harmony. The decrease in the power of harmony premitted preater freedom and boldness in the linear, con-trapuntal sphere.

치주질환 심도에 따른 조직내 림프구 및 NK 세포의 변화에 관한 면역조직학적 연구

최호근,이만섭,권영혁 慶熙大學校 齒科大學 1993 慶熙齒大論文集 Vol.15 No.1

탈회동결건조골에 혼합한 형질 변형 성장인자(TGF-β1)가 골조직 재생에 미치는 효과

정성민,이만섭,박준봉 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

The purpose of ss this study was to observe the effect of TGF-Rl on the regeneration of bone in guided bore regeneration. Four adult dogs aged 12 to 24 months were used in this study. Experimental bone defects were created surgically with surgical bur and chisel on the 3th. premolars. In experimental group; bone defect were grafted with DFDB and TGF-R1. In control groups, bone defects were grafted with only Dl-.DB. At 1, 2, 3 and 4 weeks, dogs were serially sacrificed and specimens were prepared with Hematoxylin-Eosin stain and Goldner`s stain for light microscopic evaluation. The results of this study were as follows 1. The infiltration of inflammatory cells was prominent in control groups at 1, 2 and 3 weeks. 2. The lining of osteoblast was observed at 2 weeks in control group, but at 1 week in experimental group. 3. In both groups, osteoid was formed at 2 weeks. In control groups, osteoid was fromed on only bone surface. but in experimental groups, osteoid were formed on both bone & DFDB surfaces. 4. In only experimental groups, The fusion of new bone & DFDB was only observed at 3 weeks. and the fusion of new bone & DFDG was more prominent at 4 weeks. But in control groups, No fusion of new bone & DFDB was oberved at 3 and 4weeks From the above result, the TGF-01 was effective in bone formation and increased inductive effect of DFDB guided bone regeneration technique. Inductive effect of DFDB was increased with TGF-131.

치주질환 심도에 따른 치아동요도와 교합력의 상관관계에 관한 연구

최조우,이만섭,권영혁 慶熙大學校 齒科大學 1993 慶熙齒大論文集 Vol.15 No.1

The purpose of this study was to investigate the change of tooth mobility and bite force according to periodontal disease severity. Tooth mobility and biting force due to change of viscoelastic property of periodontium were influenced by inflammation of periodontal tissue. 30 patients participated in this study, the periodontal disease severity is evaluated with SBI and attachment loss. SBI and attachment loss were examined by periodontal probe. Tooth mobility was tested two times to each tooth using periotest (Siemens Co, Germany) and bite force was evaluated with MPM-3000 (Nihon Kohden Co, Japan Statistical analysis was applied to correlation (r2) and regression analysis. The obtained results were as follows 1. As the attachment loss increased, tooth mobility increased with significance, and they had highly positive correlation (r-2=0.68) on entire dentition. 2. As the SBI increased, tooth mobility increased with significance, and they had positive correlation (r2=0.37) on entire dentition. 3. As the attachment loss increased, bite force decreased with significance, and they had highly negative correlation (r2=0.42) on maxillary anterior dentition but low negative correlation (r2=0.20) on the other portion of dentition. 4. As the SBI increased, bite force decreased with significance, and they had highly negative correlation (r-2=0.31) on maxillary anterior dentition but low negative correlation (rz=0.16) on the other portion of dentition. 5. As tooth mobility increased, bite force decreased with significance, and they had highly negative correlation (r2=0.32) on maxillary anterior dentition but low negative correlation (r2=0.16) on the other portion of dentition.

2011 대구세계육상선수권대회 남자 높이뛰기 메달리스트들의 바이오메카닉스적 특성 분석

김의환 ( Eui Hwan Kim ),배영상 ( Young Sang Bae ),김성섭 ( Sung Sup Kim ),권문석 ( Moon Seok Kwon ),위웅량 ( Ung Ryang Wo ),김기만 ( Ki Man Kim ),이정민 ( Jeong Min Lee ) 한국운동역학회 2011 한국운동역학회지 Vol.21 No.5

The purpose of this study was to perform a kinematic analysis of the high jump techniques of the three men`s medalists at the 2011 IAAF Championships in Daegu (August 27-September 4, 2011), In particular, a three-dimensional coordinates method was used to analyze the last three strides before touchdown, the touchdown techniques, and the movements after takeoff toward the bar. An analysis of the, data for the biomechanical characteristics of the world`s best high jumpers could contribute to an improvement in the performance of a national high jumper. The first conclusion of the data analysis was that the arm movements of the gold medalist, J. Williams, had a single arm form, whereas the arm movements of the other medalists were a double arm form. Second, the difference in the knee joint angles upon touchdown and toe-off was 10˚. Third, J. Williams achieved his maximum CM height after takeoff (1.26m) using the maximum flexion of his knee joint. Fourth, the foot contact duration of A. Dmitrik (0.11 s) was the shortest among the medalists, and the ratio for his transformation of horizontal velocity to vertical velocity was the greatest (75.25%) among the three. Last, the maximum CM height of T. Barry was the greatest, and his foot contact duration was the longest.

치주인대세포와 치은 섬유아세포의 혼합배양이 석회화 결절형성에 미치는 영향

인영미,박준봉,권영혁,이만섭 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The goal of periodontal therapy is to regenerate the loss of periodontal attachment appratus. Current theories suggest the cells of the periodontium have the capacity, when appropriately triggered, to actively participate in restoring connective tissues, including mineralized tissues. This study was performed to define the hard tissue regeneration effect of periodontal ligament (PDL) cells in nitro and the effect of rate of the composition in gingival fibroblasts (GF) on the hard tissue regeneration capacity of PDI_ cells. For this study, Cell growth rate, alkaline phosphatase(ALPase) levels and the ability to produce mineralized nodules in co-culture of PDL cells and GF were examined. The results were as follows ; 1. At 7 and 15 days, cell growth of co-culture of PDL and GF (50 : 50) was greater than that of PDL cells or GF alone(P<0.05). 2. Measurements of ALPase levels indicated that PDL cells had significantly higher activity when compared with that of co-culture groups and GF only(P<0.05). And, ALPase activity in 10 days was higher than that of 7 days(P<0.05). 3. The tendency of formation of the mineralized nodule were observed dose-depend pattern of PDL cells. There was statistically significant difference among group 1(PDL 100%), 2(PDL 70% GF 30%), and 3(PDL 50% = GF 50%)(P<0.01). But, there was no difference among group 3, 4(PDL 30% : GF 70%), and 5(GF 100%). 4. Also, the number of nodule was greater in co-culture of PDL 70% and GF 30% than in culture of PDL 70%(P<0.05). From the above results, it is assumed that the co-culture of PDL cells and GF stimulates the cell growth, which is not that of PDL cells but GF. And, the activity of ALPase depends on the ratio of PDL cells, and ALPase may relate to the initial phase of nodule formation. Also, it is thought that the calcified nodule formation principally depends on PDL cells, is inhibited by GF, and affected by cell density.

백서 치아 발거후 잔존 치주인대가 발치와의 치조골 재건에 미치는 영향

조성훈,허익,박준봉,이만섭,권영혁 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to observe the effects of the periodontal ligament on the healing and the formation of alveolar bone in the extraction socket, when this ligament had artificially remained in the socket during the tooth removal. Twenty rats aged 4 weeks were used and devided into the control groups (10) and the experimental groups (10) in this study. The maxillary right and left first molars were extracted in both groups. In the experimental groups the periodontal ligament was remained in the extraction sockets using 0.4% fl-aminopropionitrile, and in the control the periodontal ligament was completely removed by curettage. At 1, 3, 5, 7 and 14 days after the tooth extraction, rats in both groups were serially sacrificed. And the specimens were prepared with HematoxyIin-Eosin stain for the light microscopic evaluation. The results ofthis study were as follows ; 1. On 1 day, the periodontal Iigament was only found in the extraction socket walls of the experimental groups, and there was not the distinguishable difference between the control and the experimental groups. 2. On 3 days, there were more collagen fibers and the appearance of higher cellular density in the experimental groups than in the control. And the cells and collagen of the periodontal ligament were so actively proliferated and synthesized that invaded into the connective tissue of the extraction sockets in the experimental groups. 3. In the experimental groups, the trabecular bone was formed on the basal and lateral bone surface on 5 days. However, there was not the new bone forming appearance in the control groups at this time. 4. On 7 days, the trabecular bone was formed in the control groups. 5. On 14 days, the extraction sockets were almost entirely filled with the bony trabeculae in both groups. But, compared to the control group, the experimental groups showed the prominent differences in the amount & the density of the new bone formed. In conclusion, it was suggested that the residual periodontal ligament tissue in the extraction socket will play a major role as the important cell source in the healing and the new bone formation of the extraction socket.

배양골세포 이식이 치조골재생에 미치는 영향

정순준,허익,박준봉,이만섭,권영혁 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

This study was performed to estimate the effects of cultured bone cell inoculated on porous type hydroxyaptite for the regeneration of the artificial alveolar bone defect. In this experiment 3 beagle dogs were used, and each of them were divided into right and left mandible. Every surgical intervention were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium (30mg/Kg). To reduce the gingival bleeding during surgery, operative site was injected with Lidocaine hydrochloride (1:80,000 Epinephrine) as local anesthesia. After surgery experimental animal were feeded with soft diet(Mighty dog, Frisies Co., U.S.A.) for 1 weeks to avoid irritaion to soft tissue by food. 2 months before surgery both side of mandibular 1st premolar were extracted and bone chips from mandibular body were obtained from all animals. Bone cells were cultured from bone chips obtained from mandible with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Porous type hydroxyapatite were immerse into the high concentrated cell suspension solution, and put 4 hours for attachment the cells on the surface of hydroxyapatite. Graft material were inserted on the artificial bone defect after 3 days of culture. Before insertion of cell-inoculated graft material, scanning electronic microscopic observation were performed to confirm the attachment and spreading of cell on the hydroxyapatite surface. 3 artificial bone defects were made with bone trephine drill on Abe both side of mandible of the experimental animal. First defect was designed without insertion of graft material as negative control, second was filled with porous replaniineform hydroxyapatite inoculated with cultured bone marrow cells as expermiental site, and third was filled with graft materials only as positive control. The size of every artificial bone defect was 3 mm in diameter and 3 mm in depth. After the every surgical intervention of animals, oral hygiene program were performed with 0.1% chlorhexidine digluconate. All of the animals were sacrificed at 2, 4, 6 weeks after surgery. For obtaining histological section, tissus were fixed in 2.5% Glutaldehyde and decalcified with Planko-Rycho Solution for 72 hr. Tissue embeding was performed in paraffin and cut parallel to the surface of mandibular body. Section in Bum thickness of tissue was done and stained with Hematoxylin-Eosin. All the specimens were observed under the light microscopy. The following results were obtained. 1. In the case of control site which has no graft material, less inflammatory cell infiltration and rapid new bone forming tendency were revealed compared with experimental groups. But bone surface were observed depression pattern on defect area because of soft tissue invasion into the artificial bone defect during the experimental period. 2. In the porous hydroxyapatite only group, inflammatory cell infiltration was prominet and dense connective tissue were encapsulated around grafted materials. osteoblastic activity in the early stage after surgery was low to compared with grafted with bone cells. 3. In the case of porous hydroxyapatite inoculated with bone cell, less inflammatory cell infiltration and rapid new bone formation activity was revealed than hydroxyapatite only group. Active new bone formation were observed in the early stage of control group. 4. The origin of new bone forming was revealed not from the center of, defected area but from the surface of preexisting bony wall on every specimen. 5. In this experiment, osteoclastic cell was not found around grafted materials, and fibrovascular invasion into regions with no noticeable foreign body reaction. Conclusively, the cultured bone cell inoculated onto the porous hydroxyapatite may have an important role of regeneration of artificial bone defects of alveolar bone.