Antifungal Activity of Silver Salts of Keggin-Type Heteropolyacids Against Sporothrix spp.

Luciana Da Silva Mathias,João Carlos De Aquino Almeida,Luis César Passoni,Cristiani Miranda David Gossani,Gabriel Bonan Taveira,Valdirene Moreira Gomes,Olney Vieira-Da-Motta 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

Sporotrichosis is a chronic and subacute mycosis causing epidemiological outbreaks involving sick cats and humans in southeastern Brazil. The systemic disease prevails in cats and in humans, with the symptoms restricted to the skin of immunocompetent individuals. Under these conditions, the prolonged treatment of animals and cases of recurrence justify the discovery of new treatments for sporotrichosis. This work addresses the antifungal activity of silver salts of Keggin-type heteropolyacid salts (Ag-HPA salts) such as Ag3[PW12O40], Ag6[SiW10V2O40], Ag4[SiW12O40] and Ag3[PMo12O40] and interactions with the antifungal drugs itraconazole (ITC), terbinafine (TBF) and amphotericin B (AMB) on the yeast and mycelia forms of Sporothrix spp. Sporothrix spp. yeast cells were susceptible to Ag-HPA salts at minimum inhibitory concentration (MIC) values ranging from 8 to 128 μg/ml. Interactions between Ag3[PW12O40] and Ag3[PMo12O40] with itraconazole and amphotericin B resulted in higher antifungal activity with a reduction in growth and melanization. Treated cells showed changes in cell membrane integrity, vacuolization, cytoplasm disorder, and membrane detachment. Promising antifungal activity for treating sporotrichosis was observed for the Ag-HPA salts Ag3[PMo12O40] and Ag3[PW12O40], which have a low cost, high yield and activity at low concentrations. However, further evaluation of in vivo tests is still required.

Effects of surrounding and underlying shades on the color adjustment potential of a single-shade composite used in a thin layer

Barros Mariana Silva,Silva Paula Fernanda Damasceno,Santana Márcia Luciana Carregosa,Bragança Rafaella Mariana Fontes,Faria-e-Silva André Luis 대한치과보존학회 2023 Restorative Dentistry & Endodontics Vol.48 No.1

Objectives This study aimed to evaluate the surrounding and underlying shades’ effect on the color adjustment potential (CAP) of a single-shade composite used in a thin layer. Materials and Methods Cylinder specimens (1.0 mm thick) were built with the Vittra APS Unique composite, surrounded (dual specimens) or not (simple specimens) by a control composite (shade A1, A2, or A3). Simple specimens were also built only with the control composites. Each specimen’s color was measured against white and black backgrounds or the simple control specimens with a spectrophotometer (CIELAB system). The whiteness index for dentistry (WID) and translucency parameters (TP00) were calculated for simple specimens. Differences (ΔE00) in color between the simple/dual specimens and the controls were calculated. The CAP was calculated based on the ratios between data from simple and dual specimens. Results The Vittra APS Unique composite showed higher WID and TP00 values than the controls. The highest values of ΔE00 were observed among simple specimens. The color measurements of Vittra APS Unique (simple or dual) against the control specimens presented the lowest color differences. Only surrounding the single-shade composite with a shaded composite barely impacted the ΔE00. The highest CAP values were obtained using a shaded composite under simple or dual specimens. Conclusions The CAP of Vittra APS Unique was strongly affected by the underlying shade, while surrounding this composite with a shaded one barely affected its color adjustment.

Performance of Bemisia tabaci MEAM1 on soybean and resistance traits of cultivars

Carolina Farias e Silva Maria,de Sousa Rodrigues Arielly,Henrique Ferreira Rodrigues Raimundo,Ettore Pavan Bruno,Barboza Silva Luciana 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Bemisia tabaci MEAM1, known as whitefly, poses a significant threat to soybean crops worldwide. To develop effective pest management strategies, it is crucial to understand how this insect pest performs on different soybean cultivars and identify resistance traits in these cultivars. This study aimed to assess the biological development of B. tabaci MEAM1 and the performance of five consecutive whitefly generations on various soybean cultivars to determine the stability of resistance across multiple insect generations. Additionally, the study aimed to estimate genetic parameters associated with resistance traits. To conduct the experiment, twenty individuals of B. tabaci were released and confined within cages attached to soybean leaf petioles. The experi ment followed a completely randomized design with eleven treatments and five replicates. The emerging insects from the bioassay were confined to plants of their respective cultivars. This process was repeated for five consecutive generations of whiteflies to evaluate the cultivars’ ability to exhibit resistance across multiple generations of the pest. The results showed that the cultivars M 8808 and AS 3810 had a negative impact on B. tabaci oviposition. Additionally, the cultivar BRS 9280 influenced the development of the insect. Based on the selection index, cultivars BRS 9280, AS 3810, M 8808, and BRS 8383 exhibited resistance traits. Furthermore, cultivars BRS 9280, AS 3810, and M 8808 demonstrated resistance characteristics consistently over five gen erations when subjected to whitefly pressure. These cultivars can be considered valuable sources of resistance against B. tabaci MEAM1 for breeding programs aimed at developing resistant soybean cultivars.

Effect of the Auricular Acupoint Associated with Physical Exercise in Elderly People: A Randomized Clinical Test

Roge´ria Gonc¸alves de Carvalho Fonseca,Andre´ia Maria Silva,Luiza Faria Teixeira,Vale´ria Regina Silva,Luciana Maria dos Reis,Adriana Teresa Silva Santos 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.4

Objective: To analyze the effect of auricular acupoint associated with physical exercise on balance, mobility, and fear of falling in the elderly. Method: The study is characterized as a clinical, controlled, and randomized trial with 22 elderly people divided into two groups: kinesiotherapy group (n Z 11) and kinesiotherapy/auriculotherapy group (n Z 11). The instruments used for evaluation were Falls Efficacy Scale International; Berg Balance Scale, and Timed up and Go Test. The intervention was performed with frequency 2Â/week for 8 weeks. In the kinesiotherapy/auriculotherapy group, in addition to kinesiotherapy, auriculotherapy was applied in specific acupoints. The ShapiroeWilk test was used to determine the normality of the data, and for comparison, analysis of variance was used for repeated measures of two factors.

Tolerogenic Dendritic Cells Reduce Airway Inflammation in a Model of Dust Mite Triggered Allergic Inflammation

Luciana S. Aragão-França,Viviane C. J. Rocha,Andre Cronemberger-Andrade,F. H. B. Costa,José Fernandes Vasconcelos,Daniel Abensur Athanazio,Daniela Nascimento Silva,E. S. Santos,Cássio Santana Meira,C. 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

Purpose: The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. Methods: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte- macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme- linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. Results: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. Conclusions: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.

Ten years of minimally invasive access cavities in Endodontics: a bibliometric analysis of the 25 most-cited studies

Silva Emmanuel João Nogueira Leal,Pinto Karem Paula,Ajuz Natasha C.,Sassone Luciana Moura 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.3

Objectives This study aimed to analyze the main features of the 25 most-cited articles in minimally invasive access cavities. Materials and Methods An electronic search was conducted on the Clarivate Analytics' Web of Science ‘All Databases’ to identify the most-cited articles related to this topic. Citation counts were cross-matched with data from Elsevier's Scopus and Google Scholar. Information about authors, contributing institutions and countries, year and journal of publication, study design and topic, access cavity, and keywords were analyzed. Results The top 25 most-cited articles received a total of 572 (Web of Science), 1,160 (Google Scholar) and 631 (Scopus) citations. It was observed a positive significant association between the number of citations and age of publication (r = 0.6907, p < 0.0001); however, there was no significant association regarding citation density and age of publication (r = −0.2631, p = 0.2038). The Journal of Endodontics made the highest contribution (n = 15, 60%). The United States had the largest number of publications (n = 7) followed by Brazil (n = 4), with the most contributions from the University of Tennessee and Grande Rio University (n = 3), respectively. The highest number of most-cited articles were ex vivo studies (n = 16), and ‘fracture resistance’ was the major topic studied (n = 10). Conclusions This study revealed a growing interest for researchers in the field of minimally invasive access cavities. Future trends are focused on the expansion of collaborative networks and the conduction of laboratory studies on under-investigated parameters. Objectives This study aimed to analyze the main features of the 25 most-cited articles in minimally invasive access cavities. Materials and Methods An electronic search was conducted on the Clarivate Analytics' Web of Science ‘All Databases’ to identify the most-cited articles related to this topic. Citation counts were cross-matched with data from Elsevier's Scopus and Google Scholar. Information about authors, contributing institutions and countries, year and journal of publication, study design and topic, access cavity, and keywords were analyzed. Results The top 25 most-cited articles received a total of 572 (Web of Science), 1,160 (Google Scholar) and 631 (Scopus) citations. It was observed a positive significant association between the number of citations and age of publication (r = 0.6907, p < 0.0001); however, there was no significant association regarding citation density and age of publication (r = −0.2631, p = 0.2038). The Journal of Endodontics made the highest contribution (n = 15, 60%). The United States had the largest number of publications (n = 7) followed by Brazil (n = 4), with the most contributions from the University of Tennessee and Grande Rio University (n = 3), respectively. The highest number of most-cited articles were ex vivo studies (n = 16), and ‘fracture resistance’ was the major topic studied (n = 10). Conclusions This study revealed a growing interest for researchers in the field of minimally invasive access cavities. Future trends are focused on the expansion of collaborative networks and the conduction of laboratory studies on under-investigated parameters.

Maxillary sinus volumetric changes in jet aircraft pilots: A multislice computed tomography pilot study

Silva Yeda da,Munhoz Luciana,Filho José Rodrigues Parga,Damasceno Andreza Gomes,da Rosa Cesar Felipe França,Zukovski Eduardo Bilaqui,Teng Erik Zhu,de Castro Cláudio Campi 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.1

Purpose: This study evaluated maxillary sinus volume changes in military jet aircraft pilot candidates before and after the training program, in comparison with a control group, considering the effects of pressurization, altitude, and total flight hours, through multislice computed tomography. Materials and Methods: Fifteen fighter pilots were evaluated before initiating the training program and after the final approval. The control group consisted of 41 young adults who had not flown during their military career. The volumes of each maxillary sinus were measured individually before and at the end of the training program. Results: When comparing the initial and final volumes in the pilots, a statistically significant increase was observed both in the left and right maxillary sinuses. When evaluating the average total volume of the maxillary sinuses (i.e., the average volume of the right and left maxillary sinuses together), a significant increase in the volume of the maxillary sinuses was observed in the pilot group when compared to the control group. Conclusion: The maxillary sinus volumes in aircraft pilot candidates increased after the 8-month training program. This may be explained by changes in the gravitational force, the expansion of gas, and positive pressure from oxygen masks. This unprecedented investigation among pilots might lead to other investigations considering paranasal sinus alterations in this singular population.

Shaping ability and apical debris extrusion after root canal preparation with rotary or reciprocating instruments: a micro-CT study

da Silva Emmanuel João Nogueira Leal,de Moura Sara Gomes,de Lima Carolina Oliveira,Barbosa Ana Flávia Almeida,Misael Waleska Florentino,Lacerda Mariane Floriano Lopes Santos,Sassone Luciana Moura 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.2

Objectives: The aim of this study was to evaluate the shaping ability of the TruShape and Reciproc Blue systems and the apical extrusion of debris after root canal instrumentation. The ProTaper Universal system was used as a reference for comparison. Materials and Methods: Thirty-three mandibular premolars with a single canal were scanned using micro-computed tomography and were matched into 3 groups (n = 11) according to the instrumentation system: TruShape, Reciproc Blue and ProTaper Universal. The teeth were accessed and mounted in an apparatus with agarose gel, which simulated apical resistance provided by the periapical tissue and enabled the collection of apically extruded debris. During root canal preparation, 2.5% sodium hypochlorite was used as an irrigant. The samples were scanned again after instrumentation. The percentage of unprepared area, removed dentin, and volume of apically extruded debris were analyzed. The data were analyzed using 1-way analysis of variance and the Tukey test for multiple comparisons at a 5% significance level. Results: No significant differences in the percentage of unprepared area were observed among the systems (p > 0.05). ProTaper Universal presented a higher percentage of dentin removal than the TruShape and Reciproc Blue systems (p < 0.05). The systems produced similar volumes of apically extruded debris (p > 0.05). Conclusions: All systems caused apically extruded debris, without any significant differences among them. TruShape, Reciproc Blue, and ProTaper Universal presented similar percentages of unprepared area after root canal instrumentation; however, ProTaper Universal was associated with higher dentin removal than the other systems.

Color discrepancy of single-shade composites at different distances from the interface measured using cell phone images

Santana Márcia Luciana Carregosa,Livi Gabriella de Jesus Santos,Faria-e-Silva André Luis 대한치과보존학회 2024 Restorative Dentistry & Endodontics Vol.49 No.1

Objectives This study aimed to evaluate the impact of substrate color and interface distance on the color adjustment of 2 single-shade composites, Vittra APS Unique and Charisma Diamond One. Materials and Methods Dual disc-shaped specimens were created using Vittra APS Unique or Charisma Diamond One as the center composite, surrounded by shaded composites (A1 or A3). Color measurements were taken with a spectrophotometer against a gray background, recording the color coordinates in the CIELAB color space. Illumination with a light-correcting device and image acquisition using a polarizing filter-equipped cell phone were performed on specimens over the same background. Image processing software was used to measure the color coordinates in the center and periphery of the inner composite and in the outer composite. The color data were then converted to CIELAB coordinates and adjusted using data from the spectrophotometer. Color differences (ΔE00) between the center/periphery of single-shade and outer composites were calculated, along with color changes in single-shade composites caused by different outer composites. Color differences for the inner composites surrounded by A1 and A3 were also calculated. Data were analyzed using repeated-measures analysis of variance (α = 0.05). Results The results showed that color discrepancies were lowest near the interface and when the outer composite was whiter (A1). Additionally, Charisma Diamond One exhibited better color adjustment ability than Vittra APS Unique. Conclusions Color discrepancies between the investigated single-shade composites diminished towards the interface with the surrounding composite, particularly when the latter exhibited a lighter shade. Objectives This study aimed to evaluate the impact of substrate color and interface distance on the color adjustment of 2 single-shade composites, Vittra APS Unique and Charisma Diamond One. Materials and Methods Dual disc-shaped specimens were created using Vittra APS Unique or Charisma Diamond One as the center composite, surrounded by shaded composites (A1 or A3). Color measurements were taken with a spectrophotometer against a gray background, recording the color coordinates in the CIELAB color space. Illumination with a light-correcting device and image acquisition using a polarizing filter-equipped cell phone were performed on specimens over the same background. Image processing software was used to measure the color coordinates in the center and periphery of the inner composite and in the outer composite. The color data were then converted to CIELAB coordinates and adjusted using data from the spectrophotometer. Color differences (ΔE00) between the center/periphery of single-shade and outer composites were calculated, along with color changes in single-shade composites caused by different outer composites. Color differences for the inner composites surrounded by A1 and A3 were also calculated. Data were analyzed using repeated-measures analysis of variance (α = 0.05). Results The results showed that color discrepancies were lowest near the interface and when the outer composite was whiter (A1). Additionally, Charisma Diamond One exhibited better color adjustment ability than Vittra APS Unique. Conclusions Color discrepancies between the investigated single-shade composites diminished towards the interface with the surrounding composite, particularly when the latter exhibited a lighter shade.

Can the body composition of crossbred dairy cattle be predicted by equations for beef cattle?

Neves, Maria Luciana Menezes Wanderley,de Souza, Evaristo Jorge Oliveira,Veras, Robson Magno Liberal,de Campos Valadares Filho, Sebastiao,Marcondes, Marcos Inacio,da Silva, Gabriel Santana,Barreto, Li Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: The aim of the study was to evaluate the efficiency of the Hankins and Howe (HH46), Valadares Filho (V06), and Marcondes (M12) equations for predicting the physical and chemical composition of dairy crossbred bulls carcasses, as well as the chemical composition of their empty bodies. Methods: This study was conducted using 30 dairy crossbred bulls. One group of five animals was slaughtered at the beginning of the experiment, and the remaining were slaughtered 112 days later. Animals were distributed in a completely randomized design into treatments consisting different levels of concentrate (0%, 17%, 34%, 51%, and 68%). The physical and chemical compositions of the cattle were obtained from the right half of the carcass and using samples taken between the 9th and 11th ribs of the left half of the carcass. The estimated and experimentally determined values were compared using the correlation and concordance coefficient, as well as the mean square error of prediction (MSEP) and its components. Results: The HH46 equations were better at estimating the amount of muscle plus fat in the carcass. The amount of bone in the carcasses could not be well estimated by the HH46 and M12 models. The M12, HH46, and V06 equations were worst at estimating the amounts of protein, ether extract, and water in the carcass, respectively. In the empty body, the amounts of protein and water were well estimated by the HH46 equations. Protein, ether extract, and water were accurately estimated by the V06 equations, and ether extract by the M12 equations. Conclusion: The physical and chemical composition of dairy crossbred bull carcasses, as well as the chemical composition of their empty bodies, can be predicted using the equations tested here. The amount of bone in these carcasses could not be accurately predicted.