Antimycobacterial Activity of Fusaric Acid from a Mangrove Endophyte and its Metal Complexes

Jia-Hui Pan,Yi Chen,Yu-Hong Huang,Yi-Wen Tao,Jun Wang,Yan Li,Yi Peng,Tao Dong,Xiao-Min Lai,Yong-Cheng Lin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

Due to the increasing prevalence of multidrug-resistant Mycobacterium tuberculosis, there is an urgent need for new antituberculosis drugs that have novel mechanisms of action. As part of our ongoing search for antimycobacterial metabolites from mangrove endophytes, chemical analysis of the active extract of a strain of Fusarium sp. was performed, which led to the isolation of fusaric acid as the predominant constituent. A variety of metal complexes of fusaric acid were prepared. Antimycobacterial assays showed that Cadmium (II) and Copper (II) complexes exhibited potent inhibitory activity against the M. bovis BCG strain [minimum inhibitory concentration (MIC) = 4 μg/mL] and the M. tuberculosis H37Rv strain (MIC = 10 μg/mL), respectively. This is the first report of the antimycobacterial activity of the mangrove Fusarium metabolite and its coordinating metal complexes.

Gene expression analysis of “green tide” alga Ulva prolifera (Chlorophyta) in China

Shangang Jia,Xumin Wang,Guiming Liu,Dan Luo,Jing Zhang,Yan Liu,Xiangzhi Lin,Tao Liu 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Ulva prolifera (Ulvophyceae, Chlorophyta) is the causative species of the annual “green tides” in the Bohai Gulf of Northern China. In order to gain some more insight into the transcriptome of this fast-growing species of algae, we acquired 6,203 high-quality expressed-sequence-tags (ESTs),which were clustered into 4,080 unique sequences (UniSeqs). Functional classification revealed that most of the acquired transcripts were related to metabolic and biosynthetic processes,nucleotide binding, and cell part that might contribute to the organism’s rapid growth. Using 39 EST pools, 28 mitochondria genomes, and 18 chloroplast genomes from algae and land plants, we constructed three phylogenetic trees. These trees provided evidence that U. prolifera was in a sister position to Ulva linza, and shared a similar chloroplast origin with Pseudendoclonium akinetum. The findings of this study will improve our knowledge of the transcriptome and taxonomic position of U. prolifera.

Vacuum Carbonization of Nanometer Tungsten Powder with Carbon Black

Luo Ji,Lin Tao,Guo Zhi-meng,Jia Chengchang 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1

Vacuum carbonization of nanometer tungsten powder was investigated in a simple designed apparatus. An X-Y recorder was used to plot differential thermal analysis (DTA) curves to determine starting temperature of carbonization of four samples with different specific surface area. The product was detected by X-ray Diffraction (XRD) and small angle X-ray scattering (SAXS). The results show that finer tungsten powder has lower starting temperature of carbonization. Tungsten powder, which BET surface area is , is completely carbonized to tungsten carbide at , although the starting temperature is . Particle grows sharply before carbonization.

Polyvinylidene Fluoride Electrospun Fibers Loaded TiO2 for Photocatalytic Degradation and Oil/Water Separation

Fei Sun,Hai-Tao Ren,Shih-Yu Huang,Ting-Ting Li,Hao-Kai Peng,Qi Lin,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.7

In this study, electrospun polyvinylidene fluoride (PVDF)-based nanofibrous membranes embedding TiO2 wereprepared and used for photocatalytic degradation and oil/water separation. The nanofibrous membranes were characterizedby scanning electron microscopy (SEM), transmission electronic microscope (TEM), X-ray diffraction (XRD), Fouriertransform infrared spectroscopy (FTIR) spectroscopy, thermal performance analysis, and oil/water separation analysis. Andthe degradation of rhodamine B dye was also investigated. Results showed that the fiber diameter and thermal stability of themembranes decreased with the increasing of TiO2. Meanwhile, the fiber surface roughness and specific surface areaincreased. The analysis of TEM, XRD and FTIR indicated that TiO2 existed in the PVDF membranes. When the TiO2 contentwas 12 %, the fiber diameter of the membranes was about 110 nm, and the photocatalytic degradation of rhodamine B dyeefficiency was up to 97 %. The reaction rate constant was 0.02057 min-1. At 0.01 MPa vacuum, oil separated from watereffectively, which proves that separation can be easily conducted with a low energy cost. Thus, the prepared membranes havea very high application prospect in the purification of reclaimed water and separation of oil and water.

Lack of Association between the COMT rs4680 Polymorphism and Ovarian Cancer Risk: Evidence from a Meta-analysis of 3,940 Individuals

Du, Jin-Ze,Dong, Yu-Ling,Wan, Guo-Xing,Tao, Lin,Lu, Li-Xia,Li, Feng,Pang, Li-Juan,Jia, Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Catechol-O-methyltransferase (COMT) is involved in estrogen metabolism and is vital to estrogen-induced carcinogenesis, including that of ovarian cancer. Although many recent epidemiologic studies have investigated associations between the COMT rs4680 polymorphism and ovarian cancer risk, the results remain inconclusive. We therefore performed a meta-analysis to derive a more precise estimate of associations. Systematic searches of the PubMed, Embase, Web of Science, Cochrane Library, Wanfang, China National Knowledge Infrastructure, and Chinese Biomedicine databases were undertaken to retrieve eligible studies. Odds ratios (ORs) with their corresponding 95% confidence intervals (CIs) were pooled to assess the strength of the association. In total, 8 case-control studies involving 1,293 cases and 2,647 controls were included in the meta-analysis. Overall, the results showed no evidence of significant association between the COMT rs4680 polymorphism and ovarian cancer risk in any of the assessed genetic models. Subgroup analyses by ethnicity also did not reveal any significant association in any genetic model (p>0.05). In conclusion, our findings suggest that the COMT rs4680 polymorphism may not contribute to the risk of ovarian cancer.

Localization of QTLs for Husk Proportion and Lignin Content Using a High-Density Genetic Linkage Map of Brassica napus

Fu You Fu,Lie Zhao Liu,You Rong Chai,Li Chen,Tao Yang,Ai Fen Ma,Cun Ming Qu,Lin Jiang,Zheng Sheng Zhang,Jia Na Li 한국유전학회 2007 Genes & Genomics Vol.29 No.3

Husk proportion (HP) and lignin content (LC) are two important traits of seed quality of B. napus. Efficient selection of these two traits is an important way to improve the seed quality in meal improvement. A set of recombinant inbred lines (RILs) of B. napus (GH06 × Zhongyou 821) was used for mapping QTLs of HP and LC in a two-year study in different environments. 509 markers (78 SSR markers, 66 RAPD markers and 365 SRAP markers) were mapped on 26 linkage groups with an average length of 73.96 cM. The map covered a total of 1923 cM, and the average distance between two adjacent markers was 3.78 cM. Analysis of variance of LC and HP indicated that. LC might not be significantly affected by environment (F = 2.96 < 3.02) and HP might be affected by genotype × environment interactions. A total of 15 significant QTLs were detected in eight linkage groups with a LOD threshold value of 2.5 (LR ≥ 11.5) by CIM, explaining 4.99-16.14% of phenotype variation. Some QTLs of LC and HP were detected in near regions of the same linkage groups, such as qLCBB05-6-1 and qLCWZ06-6-1, qLCBB05-10-2 and qLCBB06-10-1 and qLCWZ06-10-2, qHPBB05-9-3 and qHPBB06-9-1, qHPBB05-10-4 and qHPBB06-10-2 and qHPBB06-10-3 and qHPWZ06-10-1. This study indicates that the repeatedly detected QTLs of LC and HP might be major-effect QTLs of LC and HP.

A Facile Method to Fabricate Bioenvironmentally Friendly Janus Nonwoven Medical Covers: Preparation and Property Evaluation

Yue Zhang,Ting-Ting Li,Bing-Chiuan Shiu,Fei Sun,Hai-Tao Ren,Xue-Fei Zhang,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2021 Fibers and polymers Vol.22 No.1

Medical product contamination is a serious threat to patients’ health. However, designing medical covers thatensure Janus performance and outstanding abrasion resistance, mechanical performances has remained a great challenge. Inthis study, a novel Janus nonwoven fabric nonwoven fabric consists of a hydrophilic inner layer of polylactic acid (PLA)/lowmeltingpolylactic acid (LPLA) and a coated outer layer of hydrophobic thermoplastic polyurethane (TPU) is successfullyfabricated. The subsequent PLA/LPLA-TPU textiles exhibits excellent protective performance of inner absorption. Moreover, the coating improves tensile strength and increases abrasion resistance. A mechanism of mechanical failure isfurther studied. This kind of nonwoven fabric is suggested to be a promising candidate for medical covers and health supplies.

Effect of 5-aza-2'-deoxycytidine on Cell Proliferation of Non-small Cell Lung Cancer Cell Line A549 Cells and Expression of the TFPI-2 Gene

Dong, Yong-Qiang,Liang, Jiang-Shui,Zhu, Shui-Bo,Zhang, Xiao-Ming,Ji, Tao,Xu, Jia-Hang,Yin, Gui-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Objective: The present study employed 5-aza-2'-deoxycytidine (5-Aza-CdR) to treat non-small cell lung cancer (NSCLC) cell line A549 to investigate the effects on proliferation and expression of the TFPI-2 gene. Methods: Proliferation was assessed by MTT assay after A549 cells were treated with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR, a specific demethylating agent, for 24, 48 and 72h. At the last time point cells were also analyzed by flow cytometry (FCM) to identify any change in their cell cycle profiles. Methylation-specific polymerase chain reaction (MSPCR), real time polymerase chain reaction(real-time PCR) and western blotting were carried out to determine TFPI-2 gene methylation status, mRNA expression and protein expression. Results: MTT assay showed that the growth of A549 cells which were treated with 5-Aza-CdR was significantly suppressed as compared with the control group (0 ${\mu}mol/L$ 5-Aza-CdR). After treatment with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h, FCM showed their proportion in G0/G1 was $69.7{\pm}0.99%$, $76.1{\pm}0.83%$, $83.8{\pm}0.35%$, $95.5{\pm}0.55%$ respectively (P<0.05), and the proportion in S was $29.8{\pm}0.43%$, $23.7{\pm}0.96%$, $15.7{\pm}0.75%$, $1.73{\pm}0.45%$, respectively (P<0.05), suggesting 5-Aza-CdR treatment induced G0/G1 phase arrest. MSPCR showed that hypermethylation in the promoter region of TFPI-2 gene was detected in control group (0 ${\mu}mol/L$ 5-Aza-CdR), and demethylation appeared after treatment with 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h. Real-time PCR showed that the expression levels of TFPI-2 gene mRNA were $1{\pm}0$, $1.49{\pm}0.14$, $1.86{\pm}0.09$ and $5.80{\pm}0.15$ (P<0.05) respectively. Western blotting analysis showed the relative expression levels of TFPI-2 protein were $0.12{\pm}0.01$, $0.23{\pm}0.02$, $0.31{\pm}0.02$, $0.62{\pm}0.03$ (P<0.05). TFPI-2 protein expression in A549 cells was gradually increased significantly with increase in the 5-Aza-CdR concentration. Conclusions: TFPI-2 gene promoter methylation results in the loss of TFPI-2 mRNA and protein expression in the non-small cell lung cancer cell line A549, and 5-Aza-CdR treatment could induce the demethylation of TFPI-2 gene promoter and restore TFPI-2 gene expression. These findings provide theoretic evidence for clinical treatment of advanced non-small cell lung cancer with the demethylation agent 5-Aza-CdR. TFPI-2 may be one molecular marker for effective treatment of advanced non-small cell lung cancer with 5-Aza-CdR.

Molybdenum trioxide impregnated carbon aerogel for gaseous elemental mercury removal

Yang Ling,Xiaokun Man,Wenbo Zhang,Daolei Wang,Jiang Wu,Qizhen Liu,Mingyan Gu,Yuyu Lin,Ping He,Tao Jia 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.4

A novel gaseous elemental mercury (Hg0) removal agent was successfully synthesized via impregnation method, by using molybdenum trioxide (MoO3) as the active component and carbon aerogel (CA) as the carrier. The as-prepared samples maintained a large specific surface area and excellent pore structure of the pure carbon aerogel, so that MoO3 was better dispersed to obtain enhanced Hg0 removal performance. The maximum efficiency of elemental mercury removal was about 74%, achieved by Mo/C500 sample at 300 oC, while it still had good ability (nearly 60%) in the range of 500-700 oC. The mechanism of mercury oxidation removal was also verified by DFT calculation. This work should help in developing suitable materials for thermocatalytic oxidation of elemental mercury, and also provide some theoretical basis and data support for full-scale application of heavy metal mercury pollution control in coalfired power plants.

Research on the Attenuation of Blast Wave by Groups of Filled Cracks in Surrounding Rock of Cavern

Xin Liu,Hong-fa Xu,Peng-xian Fan,Jian-nan Zhou,Han-sheng Geng,Jia-quan Mo,Tao-lin Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.7

This study introduces an approach for constructing groups of filled cracks in the surroundingrock to achieve anti-blasting and wave elimination protection for underground caverns. Toverify the attenuation effect of the explosion wave by the filled cracks, two models, a no-crackmodel, and a filled-crack model, were constructed using a certain proportion of analoguematerials based on the similarity theory. With the implementation of small equivalentexplosion conditions, the peak pressure of the surrounding rock of the cavern and thevibration acceleration of the cavern wall under different explosion conditions were measured. To accurately judge the attenuation efficiency of the filled cracks, the peak pressure attenuationpercentages between different measuring points were calculated. Furthermore, thecorresponding numerical simulation analysis process was performed according to the testconditions. The results show that not only the peak pressure attenuation percentages, but alsothe value of vibration acceleration, were significantly reduced due to the existence of filledcracks in the model. This indicates that filled cracks in the surrounding rock of the cavern caneffectively attenuate the peak pressure and vibration acceleration caused by the explosionwave, thereby achieving the protection engineering goal of anti-explosion and wave elimination.