Quantitative in‐line monitoring of solvent‐mediated polymorphic transformation of sulfamerazine by near‐infrared spectroscopy

Lee, Min‐,Jeong,Seo, Da‐,Young,Wang, In‐,Chun,Chun, Nan‐,Hee,Lee, Hea‐,Eun,Jeong, Myung‐,Yung,Kim, Woo‐,Sik,Choi, Guang J. Wiley Subscription Services, Inc., A Wiley Company 2012 journal of pharmaceutical sciences Vol.101 No.4

<P><B>Abstract</B></P><P>The in‐line monitoring of pharmaceutical processes with high risk, such as crystallization, has been one of the most popular research topics in recent years. Sulfamerazine (SMZ), a well‐known sulfonamide antibacterial agent was investigated to examine the mechanism of polymorphic conversion by solvent‐mediated polymorphic transformation (SMPT). The primary purpose of this study is to monitor the polymorphic transformation through in‐line near‐infrared (NIR) measurements and concurrently interpret the whole process quantitatively with off‐line characterizations. Samples taken at every hour during SMPT were analyzed by X‐ray diffractometry (XRD) and differential scanning calorimetry (DSC). NIR spectra in the range of 7500–4900 cm<SUP>−1</SUP> were taken into account for multivariate analysis, which included partial least square (PLS) regression and principal component analysis (PCA). In brief, the form II content was estimated very accurately and reproducibly during the SMPT process not only by XRD but also by the DSC measurements. In addition, the form II content values were predicted very accurately by separate experiments at two designated time points. In a separate study, it was demonstrated that PCA could be employed to explain a complicated process such as SMPT mechanistically by several stages. © 2012 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 101:1578–1586, 2012</P>

Aspirin 결정화 과정 중 특성변화의 NIR 인라인 모니터링 연구

이혜은 ( Hea Eun Lee ),왕인천 ( In Chun Wang ),이민정 ( Min Jeong Lee ),서다영 ( Da Young Seo ),신상문 ( Sang Mun Shin ),최용선 ( Yong Sun Choi ),최광진 ( Guang Jin Choi ) 한국화학공학회 2010 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.48 No.6

제약산업에서 최종의약품의 품질과 성능은 결정분말의 크기, 모양 및 다형체 등에 의해서 크게 달라지므로, 원료의 약품(API)의 결정화 공정은 매우 중요한 제약공정이다. 본 연구에서는 NIR 분광기와 광섬유 탐침을 이용하여, API 결정화 공정을 인라인 모니터링하여, 결정화 진행과정에서 핵성성, 결정성장, 다형체 등의 주요 특성을 실시간으로 감시하고 예측할 수 있는지를 탐구하였다. NIR 스펙트럼 분석에는 주요인분석법(PCA)을 적용하였고, 잘 알려진 aspirin를 대상 API로 하여 에탄올과 아세톤의 용매 혼합비율에 따른 결정특성 변화를 관찰하였다. 여러 특성분석 결과, 생성되는 aspirin 결정체의 다형은 용매 혼합비에는 무관하게 상온에서 가장 안정상인 form-I이었지만, 핵생성 개시점, 결정입도 및 결정의 형상은 용매의 혼합비에 따라 크게 달라진다는 것을 확인하였다. 이러한 결과는 NIR 스펙트럼의 PCA 해석결과와 매우 긴밀한 상관성을 보여주었다. 결론적으로, NIR 인라인 모니터링을 통해서, 약물의 결정화 과정에서 관심사가 되는 주요 결정특성을 실시간으로 관찰하고 예측할 수 있음이 실증되었다. Since the quality and performance of medicinal products are heavily dependent upon the size, shape and polymorphism of active pharmaceutical ingredients (APIs), their crystallization has been regarded as one of the most important pharmaceutical processes. In this study, NIR-based inline measurements were employed to monitor key attributes of API particles real-time during the crystallization process. Principal component analysis (PCA) method was selected to correlate inline NIR spectra while the well-known aspirin was studied as a model drug. According to our characterization results, the ratio of ethanol to acetone did not cause any change in polymorphism, but resulted in a significant difference in the nucleation time, crystal growth and crystal shape. These phenomenological changes were well correlated with the PCA`s implications. It turned out that the NIR-based inline monitoring technology can be employed well in observing and predicting key quality attributes such as crystal size during pharmaceutical crystallization processes.

당뇨병에 관한 한방적 연구 : 淸心蓮子飮, 加味調胃承氣湯, 杞菊地黃湯에 대하여 On the Cheungsimyeonjaeum, Kamijowiseunki-tang, Kikukjihwang-tang

이인자,이다미 대구효성가톨릭대학교 1992 연구논문집 Vol.45 No.1

In order to study the effect of oriental medicine on experimental diabetes mellitus and cataract, Cheungsimyeonjaeum, Kamijowiseungkitang and Kikukjihwang-tang were administered to streptozotocin indeced diabetic rats and glactose-induced cataractic rats. The results are summarized as follows; 1. Variation of body weight were significantly increased in 7th day by administration of Cheungsimyeonjaeum and Kamijowiseungkatang. 2. Weight of liver were significantly recovered by administration of Cheungsimyeonjaeum and Kikukjihwang-tang. Weight of kidney were significantly recovered by administration of Kikukjihwang-tang. 3. The consumption of water significantly decreased by administration of Cheungsimyeonjaeum, Kamijowiseungki-tang and Kikukjihwasng-tang. 4. The consumption of food were significantly decreased by administration of Chungsimyeonjaeum, Kamijowiseungki-tang and Kikukjihwang-tang. 5. Onset of cataract in experimental group was not significantly different from control group. These results suggest that Cheungsimyeonjaeum, Kamijowiseungkitang and Kikukjihwang-tang may play protective and therapaeutic roles in diabetes mellitus.

독거노인 지원 개선을 위한 서비스 디자인 제안

유다미 ( Yu Da-mi ),김혜인 ( Kim Hye-in ),박진원 ( Park Jin-won ),이사랑 ( Lee Sa-rang ),하효정 ( Ha Hyo-jung ),윤재영 ( Yun Ray Jae-young ) 커뮤니케이션디자인학회 2016 커뮤니케이션 디자인학연구 Vol.57 No.-

우리나라는 이미 2000년에 고령화사회에 접어 들었고 2026년에는 초고령사회로 접어들 것으로 예상된다. 늘어나는 노인의 수 만큼이나 독거노인과 그 문제가 심각해지고 있지만 그에 대한 지원 및 관리는 매우 부족한 실정이다. 본 연구는 이러한 문제를 해소하기 위해 독거노인의 증가에 따라 발생되는 문제를 알아보고 그 해결방안으로 봉사와 기부에 상대적으로 호의적인 청년(학생)층을 유입할 수 있는 서비스 디자인을 제안한다. 연구방법으로는 관찰조사를 통하여 독거노인, 청년(학생), 어르신 돌봄 센터와 같은 이해관계자를 도출하고, 설문조사 및 심층 인터뷰 통하여 각 이해 관계자 간에 발생하는 문제점을 발견하였다. 문제점을 해소하기 위해 크게 `봉사하기`, `기부하기` 기능으로 나누어 신청단계를 간소화하였다. 또한 시간기부인 봉사를 통해 획득한 `베리` 포인트는 물질적으로 지정기부를 할 수 있도록 기획하였다. 이와 같은 기능들을 모바일앱 프로토타입으로 제작 하였고 1차, 2차에 걸친 사용성 테스트를 진행했다. 결과적으로 본 연구가 독거노인 문제 해소와 청년들을 대상으로 한 새로운 기부문화 창출에 기여할 것으로 기대한다. Korea already became a fast aging society in 2000 and there is a forecast of becoming a super-aged society by 2026. As the number of the aged increases, the issue related with the increasing number of elderly people living alone and aging society is becoming more serious but there is an insufficiency in supporting senior citizens and managing the situation. The purpose of this study is to identify problems occurred by the increasing number of elderly people living alone to obtain a solution for the problems and suggest a service design to attract young people in their 20s, who is more likely to favor voluntary service and donation for solution. For a study method, an observational study was conducted with selecting people who are engaged in senior care center, students in their 20s, and elderly people living alone and conducting questionnaire and in-depth interview with them and the problems occurred among those interested parties. The application steps were being simplified by dividing them into major categories, `performing voluntary service` and `donating`, to solve the problems. Also, `berry` points obtained by a voluntary service, which is an essentially time-donating, is available for designated financial donation. Those functions were manufactured as a mobile application prototype and first and second usability tests were conducted. As a result, it is expected that this study will contribute to creation of new donation culture with students in their 20s and solution for elderly people living alone.

병원행정인력의 환자의료정보 비밀보호 요인 분석

김종인(Jong In Kim),이다음(Da Um Lee) 한국보건복지학회 2010 보건과 복지 Vol.12 No.-

Objectives: This paper was thus aimed at surveying consciousness of the persons working at the departments of administration, insurance (investigation) or clinical records in hospitals about security of patients' clinical information in an effort to detect related problems and to suggest alternative plans. Methods: From the departments of administration, insurance and clinical records at the hospitals located in Jeollabuk province as of 2009, 200 staff members were randomly selected as subjects for this study. Descriptive statistics were employed to determine frequency, percentage, average and standard deviation of the data and t-test and ANOVA were also utilized to analyze the factor of the subjects about security of patients' clinical information depending on their personal characters and characters of the hospitals they were working in. Results: The study says the key point is that Factors for Medical Information Protection in Medical Manpower of hospitals were too lower than general hospitals. That's because hospitals are on small scale and are not departmentalized, and their concerned security regulation, guideline or detailed curriculum are in poor condition. Conclusions: It is hoped for continuing research and investigation to pinpoint the reasons that influence consciousness of the people working at medical institutions about protection of patients' medical information. Furthermore, medical institutions are requested to take the initiative not only of providing quality and specialized medical service to patients but also of protecting safely medical information that may contain sensitive and versatile information of patients.

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

박실지의 대형수생식물상과 그 분포에 관한 연구

김인택,정선우,이다정 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

The flora(macro-hydrophyte) Vegetation of Baksil reservoir and surrounding area was investigated from March, 2005 to October, 2005. The Macro-hydrophyte of this area consist of 33 taxa : 3 variety, 30 species, 30 genera and 22 family. The Maro-hydrophyte of this area consist of Emergent Plants : 16 species, 14 genera, 11 family, Submerged plants : 4 species, 4 genera, 3 family, Free-floating Plants : 3 species, 3 genera, 2 family, Floating leaved Plants : 2 species, 2 genera, 2 family, Hygrophytes Plants : 5species, 5 genera, 4 family. The communites of Jeongyang lake : Miscanthus Sacchariflorus community, Zizania latifolia community, Phragmites communis community, Tyhpa orientalis community and Trapa japonica community. The life form spectrum : Emergent Plants 49%, Submerged Plants 12%, Free-floating Plants 9%, Floating leaved plants 6%, Hygrophytes plants 15%.

Paramolar tubercle이 융합된 상악 제2소구치의 비외과적 근관치료

최다경(Da-Kyung Choi),장훈상(Hoon-Sang Chang),황윤찬(Yun-Chan Hwang),황인남(In-Nam Hwang),오원만(Won-Mann Oh),박찬(Chan Park),이빈나(Bin-Na Lee1) 대한턱관절교합학회 2021 구강회복응용과학지 Vol.37 No.4

Ethanol Extract of Peanut Sprout Exhibits a Potent Anti-Inflammatory Activity in Both an Oxazolone-Induced Contact Dermatitis Mouse Model and Compound 48/80-Treated HaCaT Cells

( Da In Choi ),( Jee Young Choi ),( Young Jee Kim ),( Jee Bum Lee ),( Sun Ouck Kim ),( Hyong Taek Shin ),( Seung Chul Lee ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.3

Background: We developed an ethanol extract of peanut sprouts (EPS), a peanut sprout-derived natural product, which contains a high level of trans-resveratrol (176.75 μg/ml) and was shown to have potent antioxidant activity. Objective: We evaluated the potential anti-inflammatory activity of EPS by measuring its antioxidant potential in skin. Methods: The anti-inflammatory activity of EPS was tested using two models of skin inflammation: oxazolone (OX)-induced contact dermatitis in mice and compound 48/80-treated HaCaT cells. As biomarkers of skin inflammation, cyclooxygenase-2 (COX-2) and nerve growth factor (NGF) levels were measured. Results: OX-induced contact dermatitis was suppressed markedly in mice that were treated with an ointment containing 5% EPS as evidenced by a decrease in the extent of scaling and thickening (p<0.05) and supported by a histological study. COX-2 (messenger RNA [mRNA] and protein) and NGF (mRNA) levels, which were upregulated in the skin of OX-treated mice, were suppressed markedly in the skin of OX+EPS-treated mice. Consistent with this, compound 48/80-induced expression of COX-2 (mRNA and protein) and NGF (mRNA) in HaCaT cells were suppressed by EPS treatment in a dose-dependent manner. As an inhibitor of NF-κB, IκB protein levels were dose-dependently upregulated by EPS. Fluorescence-activated cell sorting (FACS) analysis revealed that EPS scavenged compound 48/80-induced reactive oxygen species (ROS) in HaCaT cells. Conclusion: EPS exerts a potent anti-inflammatory activity via its antioxidant activity in both mouse skin and compound 48/80-treated HaCaT cells in vitro. Compound 48/80-treated HaCaT cells are a useful new in vitro model of skin inflammation. (Ann Dermatol 27(2) 142∼151, 2015)

Ethanol Extract of Peanut Sprout Exhibits a Potent Anti-Inflammatory Activity in Both an Oxazolone-Induced Contact Dermatitis Mouse Model and Compound 48/80-Treated HaCaT Cells

( Da In Choi ),( Jee Young Choi ),( Young Jee Kim ),( Jee Bum Lee ),( Sun Ouck Kim ),( Hyong Taek Shin ),( Seung Chul Lee ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.2

Background: We developed an ethanol extract of peanut sprouts (EPS), a peanut sprout-derived natural product, which contains a high level of trans-resveratrol (176.75 μg/ml) and was shown to have potent antioxidant activity. Objective: We evaluated the potential anti-inflammatory activity of EPS by measuring its antioxidant potential in skin. Methods: The anti-inflammatory activity of EPS was tested using two models of skin inflammation: oxazolone (OX)-induced contact dermatitis in mice and compound 48/80-treated HaCaT cells. As biomarkers of skin inflammation, cyclooxygenase-2 (COX-2) and nerve growth factor (NGF) levels were measured. Results: OX-induced contact dermatitis was suppressed markedly in mice that were treated with an ointment containing 5% EPS as evidenced by a decrease in the extent of scaling and thickening (p<0.05) and supported by a histological study. COX-2 (messenger RNA [mRNA] and protein) and NGF (mRNA) levels, which were upregulated in the skin of OX-treated mice, were suppressed markedly in the skin of OX+EPS-treated mice. Consistent with this, compound 48/80-induced expression of COX-2 (mRNA and protein) and NGF (mRNA) in HaCaT cells were suppressed by EPS treatment in a dose-dependent manner. As an inhibitor of NF-κB, IκB protein levels were dose-dependently upregulated by EPS. Fluorescence-activated cell sorting (FACS) analysis revealed that EPS scavenged compound 48/80-induced reactive oxygen species (ROS) in HaCaT cells. Conclusion: EPS exerts a potent anti-inflammatory activity via its antioxidant activity in both mouse skin and compound 48/80-treated HaCaT cells in vitro. Compound 48/80-treated HaCaT cells are a useful new in vitro model of skin inflammation.(Ann Dermatol 27(2) 142∼151, 2015)