Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

강원도 화천군 수달(Lutra lutra) 서식지의 식생 구조

서형수 ( Hyung Soo Seo ),신영섭 ( Young Seob Shin ),이경은 ( Kyung Eun Lee ),김윤미 ( Yoon Mi Kim ),전미나 ( Mina Jeon ),남택우 ( Taek Woo Nam ),한성용 ( Sung Yong Han ),정연숙 ( Yeonsook Choung ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.special

In order to determine whether vegetation would be one of the factors for the selection of otter home range, vegetation structure and other potential factors were studied in Hwacheon, Korea. Thirteen sites, otter’s activity found and not found, were investigated in North Han River and connected tributary streams of Hwacheon-gun. Three types of vegetation were classified by cluster analysis, which is short grass, tall grass and shrub type. Vegetation zone of each channel is composed of either one type, or mosaic of tall grass and shrub type. Short grass type is common in Lake Paro and upper North Han-river where water level is highly variable throughout a year. Therefore, annual species such as Persicaria nodosa, Fimbristylis dichotomam and Chenopodium ficifolium are the most dominant. Shrub type is common at the downstream sites of Jichon stream and along mainstream of North Han River down Lake Paro. A shrub species, Salix koreensis, is the most common. Tall grass type is dominant occupying the most vegetation zone of the tributary channels. Phragmites japonica is absolutely dominant. Due to its dense cover, a few plant species are co-existed. Otter activity was found in all three vegetation types and no marked activity was found at some sites of tall grass type. There is no difference in species composition and physiognomy between tall grass sites with and without otter activity, while it shows significant difference in fish availability between two groups. Overall we found that home range of otters in the region is along the mainstream and downstream of tributary streams with high fish availability in all vegetation types and in various human activity levels.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

임신 40주와 출산 후 6주에 있는 20대 임산부 보행의 역학적 특성 비교

윤희중,문동안,정남주,금명숙,이경식,유실,김영란,한윤수 한국운동역학회 2000 한국운동역학회지 Vol.10 No.1

본 연구는 임신 40주와 출산후 6주간에 있는 임산부의 보행 동작의 특정을 규명함으로써 이와 관련된 연구자들에게 기초자료를 제공하고자 실시하였다. 이를 위해 임신 4주에 있는 임산부를 대상으로 1차 실험을 실시하였으며 동일한 대상자를 출산후 6주에 2차로 실험하여 자료를 수집하였다. 디지타이징 시 오차를 최소화하고 인체관절점의 분별을 위해 21개의 반사마커를 부착하였다. 10m의 보행주로를 걷는 동안 두 대의 비디오카메라를 이용하여60Hz로 자료를 수집하였으며 동시에 100Hz 지면반력자료를 수집하였다.수집되어진 자료는 DLT 방법을 사용하여 분석하였으며 이의 분석에 Kwon3d 프로그램을 활용하였다. 분석결과 다음과 같은 결론을 얻었다. 1. 임신 40주와 출산 후 6주의 보행 시 지지시간과 스윙시간의 비율이 다르게 나타났으며 보폭과 보간, 활보장은 임신 40주에서 더 큰 값을 보였다. 상체 전경각 역시 임신 40주에서 더 큰 값을 보였다. 힙관절, 무릎관절, 발목관절각도는 S1이 출산후 6주에서 큰 값을 보인반면 S2는 출산전후에 큰 변화를 보이지 않다. 2. 상하지면반력의 경우 FP1과 FP2는 임신 40주보다는 출산 6주에서 큰값을 보였지만 FP3는 임신 40주에서 보다 큰값을 보이고 있었다. 전후지면반력의 경우 출산전과 후 모두 Fy2에서 보다 큰 값을 보이고 있었다. This Study was to investigate kinematical characteristic comparison of the gait pregnant 40weeks and after delivery 6weeks 20´s women and provide the basic data. To accomplish this study, in the first, carry out an experiment with the pregnant women in the pregnant 40weeks on the videotape, and than the second, make an experiment with the kinematic data. Kinematic data were filtered with a low-pass, fourth-order Butterworth filter with an effective cut off frequency of 6Hz. The conclusions were drawn as follows; 1. Floor contact Periods(stance and swing rate) were differed from before and after the delivery and a pregnant 40 weeks was greater than after delivery 6 weeks in the step, width, stride. Also, a pregnant 40 weeks was greater than after delivery 6 weeks in the front angular. And the other angular components were represented similar pattern. 2. In the vertical load, delivery 6 weeks was grater than a pregnant 40 weeks in the FP1(first peak) and FP2(valley). And FP3(second peak)was represent that a pregnant 40 weeks was greater than delivery 6 weeks. Also, in the horizontal shear, Fy2(progressional force)was greater than Fy1(breaking force) about time of the delivery.

위암환자에서 BrdU에 의하여 유도되는 Fragile sites의 발현

윤숙자,한승로,정옥,김수일,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

The expression frequency of BrdU-induced fragile sites was examined in stomach cancer patients to determine whether this parameter could be used as a marker of genetic susceptibility in at-risk individuals. G banding performed to detect fragile sites. No difference was found in expression frequency between the stomach cancer patients and a group of normal individuals(p=0.7). This indicates that the expression frequency of BrdU-induced fragile sites is not a suitable marker for assessing genetic susceptibility in stomach cancer patient.

치성 낭종의 임상-병리학적 소견과 TGF-α 및 TGF-β 발현

한경민,김용환,박진혁,윤형기,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Periapical cyst is a relatively common disease but heterogenous in histogenesis, and inflammatory reaction and fibrosis of varying degree are associated in the cystic wall. The aim of thes study is to evaluate the clinico-pathologic characteristics such as patient's age, ses, location, and the degree of inflammatory reaction and fibrosis of periapical cyst and the role of TGF-α and TGF-β cytokines in the inflammed periapical cyst. Methods and Material : The materials were 43 cases of periapical cyst and clinical information with age, sex, location(maxilla vs mandible, right vs left, region involved) were recorded. Histopathological examination on the degree of inflammatory reaction and fibrosis and immunohistochemical stining for TGF-α and TGF-β were perfomed. Satistical analyses between clinical parameters, the degree of inflammation and fibrosis and expressions for TGF-α and TGF-β were followed. Results : 1. Thirt(69.8%) of 43 cases were older than 20 year-old. and no significant differences of incidence according to patient's sex was noted. In 25(58.1%) out of 43 case. 20(54.1%) out of 37 cases, and 20(48.8%) of 41cases, the cyst was located on the maxilla. in right side and in premolar region respectively. 2. The cysts of developmental and inflammatory origin were 24(55.8%) and 19(44.2%) of 43 cases, respectively and there were no siginificant differences of patient's age, sex, and location of cysts according to the origin of cysts. 3. Fibrosis was more conspicuous in cases older than 20 year-old. (p=0.0179), but no significant difference in the degree of inflammatory reaction according to patient's age and in the degree of inflammatory reaction and fibrosis according to sex and location of the cysts were seen. 4. Inflammatory reaction was slightly more in cysts of inflammatory origin, but no significant defferences in degree of inflammation and fibrosis according to origin of the cysts. 5. The degree of inflammatory reaction and TGF-α and TGF-β expressions showed a tendency of positive correlation(p=0.1563), but there were no statistically significant relationships between the degree of inflammation and fibrosis and TGF-α and TGF-β expressions. 6. In the cysts of developmental origin, TGF-α expression in the epithelial cells was significantly increased than inflammatory origin, but TGF-α and TGF-β expressions in the inflammatory cells and stromal cells showed no significant difference according to the origin Conclusion : The degree of inflammatory reaction and fibrosis of periapical cysts was different according to patient's age and location of the cyst. TGF-α and TGF-β cystokines seemed to be involved in the inflammatory reaction and fibrosis of periapical cyst, however, TGF-α and TGF-β expressions was not parallel to the degree of inflammatory reaction and fibrosis. These results suggest that other cytokines in addition to TGF-α and TGF-β may also be involved in the inflammatory reaction and fibrosis of periapical cyst. Increased TGF-expression in the epithelial cells of the cysts of developmental origin could be a helpful finding to defferentiate the origin of the periapical cyst.

직분식 디젤엔진용 연료분사계에 따른 분사특성과 엔진성능에 관한 연구

윤천한,김경훈 경희대학교 레이저공학연구소 2004 레이저공학 Vol.15 No.-

This study has focused on using fuel injections as variables for measuring performance and reducing exhaust gas in turbo-charger inter-cooler diesel engine. In experiments, we changed nozzle hole diameter, diameter of an injection pipe, and injection timing as variable. The results show that torque, fuel consumption and smoke are reduced as nozzle hole diameter decreases, while NOx increases. When the diameter of injector is reduced, torque, fuel consumption and smoke are deteriorated, but NOx is decreased. In addition, when the time for injection is advanced, torque, fuel consumption and smoke are improved, but the density of NOx is increased.

운동처방에 의한 써키트웨이트트레이닝이 심박수 및 근력에 미치는 효과

윤상화,이한경 龍仁大學校 1991 용인대학교 논문집 Vol.7 No.-

The students who had experience of the physical education 12 majors and 12 non-majors have been chosen at discretion and trained for 12 weeks so as to examine the effect of Circuit and Weight training by exercise Prescription on their heart rate and muscular strength. And the results are as follows. 1. Heart Rate in the stable Conditions reflected G-1(-2.06 beats /min) and it showed the significant of (p<0.001) at the point of G-2(-4.08 beats /min). 2. Left Grip Strength showed the significant increase of (p <0.001) rate at both G-1(+3.95kg), G-2(+3.5kg) and Right Grip Strength showed the significant increase of (p<0.001) rate at both G-1(+3.87kg), G-2(3.69kg), Back Muscule Strenght in case of (p<0.001) rate at both G-1(+l7.86kg), G-2(17.44kg).

AE기법 이용한 JLF/STS304이종재료의 최적 마찰용접조건에 관한 연구

윤한기,이진경,이상필,공유식 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.2

저방사화 페라이트강인 JLF는 핵융합로의 블랑켓과 같은 열교환기의 재료로써 유용한 재료이다. 이 재료의 광범위한 적용을 위해서는 JLF강과 다른 재료와의 접합기술의 개발이 중요하다. 최근 두 이종재료간의 접합에는 마찰용접이 많이 이용되고 있으며 본 연구에서도 JLF강과 STS304 간의 마찰용접에 의한 용접특성을 평가하였으며 특히 비파괴기법중의 하나인 음향방출 기법을 이용하여 두 이종재료간의 용접특성 및 최적의 용접조건을 평가하였다. 음향방출 파라미터중의 하나인 카운트와 사상수 등이 최적용접조건 및 용접품질을 평가하는데 유효한 파라미터들이었다. Japanese low activation ferritic steel(JLF) is a good material for the parts of heat exchanger such as blanket and diverter. At first, JLF was developed as a candidate for structural materials in nuclear fusion applications. However, the development of the jointing technique of JLF steel to other materials is important for wide applications of this material to the industry fields. Recently the jointing technologies including diffusion bonding, brazing, roll bonding, explosive bonding and hot iso-static pressing have been studied for the heterogeneous materials of JLF-1 steel(Fe-9Cr-2W-V-Ta) and stainless steel(STS304). Friction welding is one of the most popular welding methods for two different kinds of materials. In this paper, the JLF-1 steel was jointed to STS304 by friction welding method and the optimal conditions of the friction welding discussed. Acoustic emission was used as a nondestructive technique to evaluate the weld quality in processing.

요골동맥을 이용한 관동맥 조영술의 유용성

윤정한,이승환,이한요,김장영,김일회,최윤종,이형준,이명옥,김승년,황성오,홍인수,최경훈 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.10