그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.

결핵균체 단백항원에 대한 항결핵균 IgG 항체에 관한 연구

최대경,백태현,권지순,심운택,이동배 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

In order to study the level of IgG antibody binding to sonicate antigen of Mycobacteriumn tuberculosis H_34Rv for ammonium sulfate 30∼40 fraction in sera isolated from 209 patients and. 135 controls were measured by plastic tube enzyme-linked immunosorbent assay (ELISA). The levels of IgG antibody binding to Mycobacterium tuberculosis H_37Rv antigen in sera from all patients and controls were measured after absorption of 1 : 400 diluted sera with ,Wycobacteriuez bovis and dMlycobacterium Kansasii for ammonium sulfate 30∼40 fraction. The authors conducted the survey from August 1984 to January 1985. The results obtained were as follows : 1. At the serum dilution of 1:400, the mean ELISA values of IgG antibody to Mycobacterium tuberculosis H_37Rv after absorption with Mycobacterium kansasii antigen from far advanced patients and control were 0.8666±0.4903 and 0.2058±0.1353, respectively. 2. The mean ELISA value of IgG antibody to Mycobacterium tuberculosis H_37Rv afer absorption with Mycobacterium kansasii antigen from moderate patients was 0.6488±0.3918, the ratio of ammonium sulfate fraction 30∼40 in sera from patients and controls was 3.153. 3. The mean ELISA value of IgG antibody to Mycobacterium tuberculosis H_37Rv after absorption with Mycobacterium kansasii antigen from minimal patients was 0.4205±0.3155, the ratio of ammonium sulfate fraction 30∼40 in sera from patients and controls was 2.043.

자궁경부 세포진 검사에서 비정형 선세포의 임상병리학적 중요성

서동수,윤만수,최경운 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

체질량지수에 따른 여고생의 영양 섭취에 관한 연구

전재영,김태운,백영호,최문기,이경희 釜山大學校 附設 體育科學硏究所 2006 體育科學硏究所 論文集 Vol.22 No.2

The purpose of this study was to investigate the daily nutrition intake of high school girl students according to the BMI. The subjects of this study were classified into under weight group; UG(<14.9percentile; n=9), normal weight group; NG(15.0~84.9percentile; n=12), and obese group; OG(>85percentile; n=12) by BMI.......

중 고등학교 수학교육 개선을 위한 연구

邊龍聖,金東碩,邊東壽,崔雲行,洪慶和,鄭寶鉉 弘益大學校 1991 弘大論叢 Vol.23 No.2

A great many students are working for their mathematics learning in secondary schools and nevertheless its learning effect is rather low. This phenomenon is largely due to a biased social recognition. Furthermore the educational environment including big class size and ability differences enlarge such a difficulty. This study aims at seeking an improving device to enhance learning efficiency under the circumstances. Questionaires about problem finding of teachers educational activity and mathematics teaching were distributed and collected. Data were analyzed to establish a desirable mathematics education and teaching. Devices for individualized instruction and learning tasks and evaluation methods were proposed. This recommendations are supposed to be an improving way for students' mathematics learning. In relation to this result, a further study is strongly required and adminstrative supports by government are badly needed to achieve such a goal.

${\alpha}$-Glucosidase Inhibitiors from Seed Extract of Paeonia lactiflora

Choi, Chun-Whan,Choi, Yeon-Hee,Cha, Mi-Ran,Park, Jee-Hee,Kim, Young-Sup,Kim, Young-Kyoon,Choi, Sang-Un,Yon, Gyu-Hwan,Hong, Kyung-Sik,Kim, Young-Ho,Ryu, Shi-Yong The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6

Alpha-glucosidase inhibitors are oral antihyperglycemic agents that act on alpha-glucosidase competitively, delaying intestinal carbohydrate absorption and lessening postprandial increases in glucose levels. In the search for new classes of ${\alpha}$-glucosidase inhibitors extracted from natural resources, 420 different plant extracts were evaluated for their inhibitory effect on ${\alpha}$-glucosidase, in vitro. Amongst the tested extracts, the seed extract of Paeonia lactiflora (Paeoniaceae) demonstrated a significant degree of inhibition on the enzyme, according to dose. Extensive bioassay-guided fractionation of the extract resulted in the isolation of six materials, luteolin (1), resveratrol (2), trans-${\varepsilon}$-viniferin (3), gnetin H (4), suffruticosol A (5) and suffruticosol B (6), that are active agents for ${\alpha}$-glucosidase inhibition. Resveratrol (2) is a well-known naturally occurring hydroxystilbene, particularly abundant in wine and some related stilbene oligomers. Materials (3-6) exhibited relatively strong inhibition of ${\alpha}$-glucosidase and their $IC_{50}$ values were calculated as: 0.92mM (2), 3.15mM (3), 1.15 mM (4), 2.53mM (5) and 3.15mM (6). In contrast, the ($IC_{50}$) value of the reference drug, acarbose, was estimated as 8.28mM in vitro.

Lysophosphatidic acid‐induced expression of periostin in stromal cells: Prognoistic relevance of periostin expression in epithelial ovarian cancer

Choi, Kyung Un,Yun, Jeong Sup,Lee, Il Hwan,Heo, Soon Chul,Shin, Sang Hun,Jeon, Eun Su,Choi, Yoon Ji,Suh, Dong‐,Soo,Yoon, Man‐,Soo,Kim, Jae Ho Wiley Subscription Services, Inc., A Wiley Company 2011 International journal of cancer: Journal internati Vol.128 No.2

<P><B>Abstract</B></P><P>Lysophosphatidic acid (LPA) is a bioactive lipid crucial for the initiation and progression of ovarian cancer. Identification of LPA‐induced biomarkers is necessary for predicting prognosis of ovarian cancer patients. Here we report periostin, an extracellular matrix protein, as an LPA‐induced protein in stromal cells and as a prognostic marker in patients with epithelial ovarian cancer (EOC). In human EOC tissues, periostin was mainly expressed in cancer‐associated stromal fibroblasts, but not in cancer cells. The expression levels of periostin highly correlated with poor survival and tumor recurrence of ovarian cancer patients. Treatment of human adipose tissue‐derived stromal cells with LPA or conditioned media from human ovarian adenocarcinoma cell lines, such as SK‐OV‐3 and OVCAR‐3, induced expression of periostin. The periostin expression induced by cancer‐conditioned media was abrogated by silencing of the LPA receptor 1 expression using small hairpin RNA lentivirus. Recombinant periostin stimulated adhesion and invasion of SK‐OV‐3 human ovarian adenocarcinoma cells and induced expression of matrix metalloprotease‐2 in the cancer cells. These results suggest that LPA is associated with the expression of periostin in cancer‐associated fibroblasts of EOC.</P>

배양산삼 추출액의 Doxorubicin 독성 완화 효과

최경운 ( Kyung Un Choi ),이평재 ( Pyeong Jae Lee ),김호현 ( Ho Hyun Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.3

Objectives: This study was aimed to define the protective effect of Tissue Cultured Root of Wild Ginseng (CWG) against doxorubicin (Doxo) toxicity, and investigate the anti-tumor synergic effect of CWG in combination with Doxo in tumor-bearing C57BL/6 mice. Methods: Tumor-bearing mice were established by single inoculation with B16/F10 melanoma cells (2×10(6)/㎖) subcutaneously. Tumor-bearing mice (tumor volume between 50-100 ㎣) were selected and divided them into control, Doxo, and Doxo+CWG group. Mice of Doxo group were received with Doxo (4 ㎎/㎏ of B.W.) intraperitoneally at 0, 4, 8 days after starting the experiment. Mice of Doxo+CWG group were received CWG water extract during 12 days in combination with Doxo treatment. The body weight, tumor volume, tumor weight, and organ weight (heart, liver, kidney, and testis) were measured. And serum SPK, GOT and creatinine values were analysed. Results: The volume and weights of tumor masses in Doxo group were decreased significantly compared with the those of control group. And the those of Doxo+CWG group were not significantly different from the those of Doxo group. Whereas the weight of body, liver, kidney and testis in Doxo+CWG group were increased significantly compared with the those of Doxo group. The level of serum CPK and GOT in Doxo group were increased compared with the those of control group. But the value of Doxo+CWG group were decreased significantly compared with the values of Doxo group. Conclusions: These results suggest that CWG has protective effect against doxorubicin toxicity. And these effect is guessed that is caused in augmentation of vital energy.

Horst Frenz ( ed ) . , Nobel Lectures ; Literature 1901 - 1967

최은경 ( Un Kyung Choi ) 한국영어영문학회 1976 영어 영문학 Vol.59 No.-

Peptide YY producing strumal carcinoid tumor of the ovary in a postmenopausal woman: a rare cause of chronic constipation

( Hye Kyung Noh ),( Byung Su Kwon ),( Yoon Hwa Kim ),( Nam Kyung Lee ),( Kyung Un Choi ),( Dong Soo Suh ),( Dong Hyung Lee ),( Ki Hyung Kim ) 대한산부인과학회 2017 Obstetrics & Gynecology Science Vol.60 No.6

Strumal carcinoid tumor of the ovary is a rare subtype of ovarian carcinoid tumors; it is characterized by an intimate mixture of thyroid and carcinoid tissues. We present a case of a 64-year-old woman who presented with the chief complaint of persistent, severe constipation for over 5 years; she was later found to have an ovarian strumal carcinoid tumor. Computed tomography showed a well-defined solid mass measuring 6.4 cm at the right adnexa. The patient underwent right salpingo-oophorectomy and was histopathologically diagnosed as having a strumal carcinoid tumor. Immunohistochemical examination showed immunoreactivity for peptide YY (PYY), which exerts an inhibitory effect on the peristaltic actions of the distal intestine. After surgery, the patient's constipation resolved rapidly, suggesting a correlation between PYY producing ovarian carcinoid tumor and constipation. This is the first case report of PYY producing primary strumal carcinoid tumor of the ovary associated with persistent, severe constipation from Korea.