柴胡가 free radical에 의한 脂質過酸化物 生成에 미치는 效果

문진영,최미정,남경수,임종국 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

Antioxidant effects of aqueous Siho extract on lipid peroxidation were determined by TBA value and also by lipid peroxidation value during the autoxidation of Iinoleic acid. Siho extracts exhibited markedly antioxidant aCtivity, which inhibited 99% of linoleic acid peroxidation. And Siho extracts showed 42% scavenging effect on α,α-dlphenyl-β-pjcrylhydrazyl radical, also exhibited a 60-94% inhibitory effect on superoxide generation. Siho extracts dose-dependently protected the cell death induced by tert-butyl hydroperoxide (t-BHP) and significantly increased cell viability In the normal rat liver cell(Ac2F) . These results suggested that Siho might play a protective role in lipid peroxidation by free radicals. Key Words: lipid peroxidation ; Siho extracts; scavenging effect

중·고등학생들의 보건교육 요구도 조사연구

문기내,남철현,김귀희,위광복,박경옥,이미경,심규범,장영진 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

This study was conducted to examine middleㆍhigh school 720 students in seven regions of middleㆍsmall city and town, Myon for 2 months from March 2 through April 30, 2002 in order to realize a side of school health education in middleㆍhigh school and health education need as well as health education specialist in school in the future. The results of this study can be summarized as follows. 1. Health condition is now 'normal' is 51.0%, 'good' is 45.0%, 'not good' is 4.1% and health condition of children is good as much as education level of mother is higher. 2. Experience to learn health education in school for one year recently is 'no' is 39.6%, 'don't know' is 32.6%, 'yes' is 27.8% and it represents health education in school doesn't work well and experience to learn health education is lower and lower in town and Myon. 3. Health education ways to work in school now are 'Movie, Video, Slide' is 40.7%, 'education as groups of nurse-teacher' is 38.6%, 'to distribute health education material and book' is 7.7%, 'education by setting an example' is 3.4%, 'the others' is 9.7%. 4. Time of appropriate health education for one time is 'about a hour' is 56.7%, 'about 30 minute' is 33.8%, 'about two hour' is 7.6%, it represents this situation is because of what students' time is lack and burden about a curriculum in an education administratin and school classed of a focused school score. 5. The average score of satisfaction of health education in school is 16.99±0.58, 'men' is 1.79±0.60, 'women' is 1.61±0.55 as the distinction of sex, satisfaction level of men is higher than women. 6. Learning route about health information is 'through mass media' is 59.9%. 'through family, friend' is 15.0% 'health education lecture' is 7.2%, 'printing media' is 6.1%, 'medical institution' is 5.6%, 'the others' is 5.1%, 'public health center' is 1.0%, 'through mass media' is the highest percent as each 59.1% and 60.6% in all of men and women. 7. The most efficient education way of health education is 'movie, video, slide' is 32.6%, 'health education specialist who works school' is 21.9%, 'school health education by nurse-teacher' is 16.0%, 'way through mass communication' is 11.6%. 8. The appropriate health education specialist in the future is 'health education specialist who has qualifications' is the highest as 64.5%, 'nurse-teacher' is 12.4%, 'medical personnel(doctor, a physician of oriental medicine etc.,)' is 11.0%, 'staff in public health center' is 7.2%, people in other regions(health and medical professor etc.,) is 7.0%, it represents school health education is not actual and is formal now in second hand. 9. The most necessary health education program is 'program of stress solution' is the highest as 24.9%, 'program of no smoking' is 22.7%, 'sexual education program' is 19.8%, 'school violence program' is 8.8%, 'isolate from their friends prevention program' is 8.0%, solution program of stress which gives student by school score was very necessary. Therefore, school health education is formal and inefficient because school education focuses on an entrance exam, so middleㆍhigh school students decrease concern and interest about health education. Furthermore, reliance is gotten down about nurse-teacher who takes charge of school health, a charged person of school health education wants more chargeable health education staff. And a solution program of stress that gives students by school score was very necessary. According to active concern and planned, systematic school health education is necessary to give right health knowledge, health maintenanceㆍpromotion and setting of appropriate health education specialist is very urgent.

Phospholipase A₂활성억제와 관련한 시호 물추출액 및 시호 약침액의 항염증효능에 관한 연구

문진영,남경수,임종국 동국대학교 경주대학 1997 東國論集 Vol.16 No.2

농도별 시호 약침액과 시호 물 추출액을 대상으로 phospholipase A_2(PLA_2)에 대한 시호의 영향을 알아보기 위하여, 1-stearoyl-2-[1-^14C]arachidonyl-sn-glycerophosphocholine을 기질로 하여 Ⅰ형 PLA_2(porcine pancreas) 및 Ⅱ형 PLA_2(Crotalus atrox)의 활성에 대한 효과를 측정하여 다음과 같은 결과를 얻었다. 1) PLA_2 효소의 활성발현에 최적 배양시간이 어떠한지를 알아보기위해 pH 7.0에서 37℃에서 반응시간을 0분에서 180분간의 범위까지 기질의 농도를 20n㏖로 하여 porcine pancreas 및 Crotalus atrox 유래의 PLA_2를 대상으로 활성의 변화를 관찰한 결과, 반응시간이 길어질수록 두 효소의 활성이 점차로 증가하였다. 2) Porcine pancreas 유래의 Ⅰ형 PLA_2의 활성에 미치는 시호의 영향을 관찰한 결과, 시호 약침액은 최고 92%, 시호 물 추출액에서는 최고 94%의 PLA_2 활성 저해효과를 보였으며, 희석배수가 증가할수록 활성 저해효과는 감소하였다. 3) Crotalus atrox 유래의 Ⅱ형 PLA_2의 활성에 미치는 시호의 영향을 관찰한 결과, 시호 약침액은 최고 60%, 시호 물 추출액에서는 최고 55%의 PLA_2 활성 저해효과를 보였다. 또한 시호 약침액 및 물 추출액의 원액에서 4배 희석액까지는 40% 이상의 억제효능을 보인 반면, 8배 이상의 희석액부터는 활성 억제효능이 급격히 감소하였다. 이러한 결과로 미루어 볼 때 시호 약침액 및 시호 물 추출액은 모두 PLA_2에 대한 강한 억제활성이 있음을 알 수 있었다. 또한 PLA_2 효소의 유형별로 볼 때, 시호는 Ⅱ형 PLA_2에 대해서 보다 Ⅰ형 PLA_2에 대한 억제효능이 훨씬 강함을 알 수 있었다. 그리고 시호 약침액과 시호 물 추출액을 비교해보면 Ⅰ형 PLA_2에 대해서는 시호 물 추출액이, Ⅱ형 PLA_2에 대해서는 시호 약침액이 상대적으로 다소 강한 억제효능이 있음을 알 수 있었다. 본 연구의 결과에서 시호는 그 효능적 측면에서 항 염증제, 특히 PLA_2 저해제로 개발될 수 있는 가능성이 제시된다. Phospholipase A_2(PLA_2) is a key enzyme that catalyze the hydrolysis of the ester at the sn-2 position of cell membrane phospholipid to produce a lysophospholipid and arachidonic acid. Lysophospholipid is the precursor of platelet-activating factor(PAF). And arachidonic acid is the substrate for cyclooxygenase and lipoxygenase in the arachidonic acid cascade generating various eiconanoids such as prostaglandins, leukotrienes and thromboxanes. Thus, PLA_2 plays an important role in the pathogenesis of inflammation. Recently, compounds that inhibit PLA_2 activity have been received considerable attention as potential therapeutic agents in the treatment of inflammation. Bupleury radix has been used as a natural drug for fever, inflammation and liver disease in traditional medicine. This study was performed in order to investigate the inhibitory effects of Bupleury radix aqueous extract(BRAE) and aqua-acupuncture solution(BRAA) on activities of two different types of PLA_2. BRAE showed about 94% inactivation of group Ⅰ PLA_2 from porcine pancreas, and 55% inactivation of group Ⅱ PLA_2 from Crotalus atrox ultimately. And BRAA exhibited about 92% inactivation of group Ⅰ PLA_2, and 60% inactivation of group Ⅱ PLA_2. On the basis of these results, it is suggested that Bupleury radix might play a anti-inflammatory role by a mechanism that probably involves inactivation of PLA_2.

경사 슬래브교의 지간/폭, 경사각에 따른 응력 상관성

남문희,정희효,이관희,이수경 慶尙大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.12 No.-

Skewed slab bridge have designed a width beam, stress of skewed slab bridge is quite different in according with skew and width /length. Especially in case that we analyse skewed slab bridge as a width beam, stress of right angle direction against axis of bridge is not analysed. In this study we will present the stress of transverse axis to longitudinal axis of bridge according to skew and width/length.

아동의 문제행동 발견과 프로그램 개발

문선화,김영호,이경남,장수한 부산대학교 사회복지연구소 2000 社會福祉硏究 Vol.10 No.-

라오스의 벼 생산 현황과 문제점

이문희,최경진,이정일,정남진,양원하,김제규 한국국제농업개발학회 2001 韓國國際農業開發學會誌 Vol.13 No.4

Rice is the single most important crop in Lao PDR. In the 2000 rice production year, total rice production in the Laos reached 2.2 million tons. This is regarded as sufficient to provide national self-sufficiency. The rice harvested area in 2000 was approximately 719,500ha and represented 80% of cropped land area. Approximately 82% of production was from wet season cropped and 18% was from the irrigated dry season. Grain yield per unit area vary from 1.68 to 4.39 ton/ha in the wet season environment. However, the highest grain yield (4.39 ton/ha) obtained from the dry season irrigated crop. Higher yields and reduced year variation in production can be expected with the further intensification of production systems in the low land environments. However, further imporvements in production will be dependent on higher levels of inputs and further alleviation of some the production constraints.

모기업 협력업체 산업안전보건관리에 대한 인식

이경용,이관형,오지영,서남규,손두익,갈원모,신문진 한국안전학회 2003 한국안전학회지 Vol.18 No.4

This study is planned to investigate the attitude toward the safety and health management of contractor comparry. Under the contract based production system, all of activities including safety and health management in the contractor company are depended upon the contract. How to make contract influence the worker's health of contractor company. Work's health of contractor company can be protected by efforts of company of contract-out and contractor company, especially their safety managers. The modelling of the effective safety and health management system for contractor company should consider the need of safety manager of each company and employer of contractor company. Data is collected from safety managers of 3 contract out companies as electronic and electrical manufacturing industry and 55 safety managers, 57 employers of their contractor companies using self administered survey with structured questionnaire. The most of all respondents want to support from the contract out companies. The most important items supported from contract out company is the information based on the safety information network between each company. Safety manager and employer of contactor company also itemized safety education and training in the supporting system from contract out company. These results can be generalized to survey on the other industries.

PC를 이용한 연삭가공용 전문가 시스템의 개발

김남경,황기문 진주산업대학교 1993 論文集 Vol.32 No.-

This paper treats the construction of expert system for establishment of operation condition in grinding, by getting the data from workshop and databook. This paper describes the basic conceptions for the grinding expert system, and present an architecture and basic strategy for grinding expert system. Moreover, The practical examples of a implementation is described. By the system, unskilled workers will be able to make use of the knowledge and experience of skilled workers. The system includes programs that, ⑴selection of the best wheel from among avairable wheel, ⑵ determine depth of cut, feed, wheel speed and work speed ⑶indicate routine for solution of trouble in grinding process. The developed system perform in using the CLIPS as a software tool and run under the IBM PC/AT as a hardware tool.

당뇨병 환자에서 혈장 Thrombin-Antithrombin Ⅲ 및 Plasmin-α_2-Plasmin Inhibitor 복합체의 임상적 의의

김경욱,김은숙,정상수,윤수지,박우일,이준희,남수연,안철우,문병수,김경래,차봉수,송영득,임승길,이현철,허갑범 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:당뇨병 환자에서 혈액응고 및 섬유소용해 체계의 이상경향이 있어 그 결과로 여러 혈관합병증의 발생위험이 높다는 사실은 널리 알려져 있다. 그 기전은 아직 확실히 밝혀지지 않았으나, 고혈당으로 인한 혈장 단백질들의 비효소성 당화작용이나 산화성 스트레스로 인한 유리 라티칼 작용으로 응고항진이나 섬유소용해 활성의 저하를 유발하는 것으로 생각되고 있다. 최근 응고 및 용해인자와 그 억제자의 복합체들의 증가가 이 상태를 비교적 예민하게 반영한다고 알려져 있다. 방법:본 연구에서는 당뇨병 환자 101명과 정상 대조군 20명에서 혈장내 thrombin­antithrombin complex(TAT)와 plasmin­α₂­plasmin inhibitor complex(PIC)를 측정하여 비교하고, 당뇨병 환자에서 미세혈관 합병증과 대혈관합병증의 유무에 따른 차이와, 이미 혈관 질환의 위험인자로 알려져 있는 인자들간의 상관성을 알아보고자 하였다. 결과:1. 환자의 분포를 살펴보면 혈관합병증이 있는 군은 85명, 혈관합병증이 없는 군은 16명이었고, 평균연령은 각각 57.9±14.1세, 49.9±16.6세로 혈관 합병증이 있는 군에서 더 나이가 많았고, 체질량지수는 23.2±3.4㎏/㎡, 24.1±3.4㎏/㎡로 두 군간 유의한 차이는 없었다. 또 두 군간의 혈압 및 HbA1c, 공복혈당 및 인슐린과 C­peptide, 총 콜레스테롤, 중성지방, HDL­콜레스테롤, Lp⒜는 유의한 차이가 없었고, 미세혈관합병증이 있는 군에서 당뇨병의 유병기간이 길었다. 2. TAT 및 PIC의 농도는 정상 대조군에서는 2.8±1.2 ng/mL, 240.4±69.7 ng/mL이었고, 당뇨병 환자군에서는 9.5±22.6 ng/mL, 472.2±258.7 ng/mL이었다. TAT와 PIC 모두 당뇨병 환자군에서 정상 대조군에 비해 유의하게 증가되어 있었고(p<0.001), TAT/PIC ratio는 두 군간 차이가 없었다. 3. 당뇨병 환자의 혈관합병증에 따른 TAT 및 PIC, fibrinogen 농도는 합병증이 없는 군은 각각 4.1±2.4ng/mL, 362.2±272.0ng/mL, 322.7±102.4mg/mL으로 PIC와 fibrinogen의 증가를 보였으나, 연령을 보정한 후에는 통계학적 유의성은 없었다. 또 대혈관 합병증군에서는 각각 6.0±4.9 ng/mL, 507.4±321.6 ng/mL, 427.1±194.7 mg/dL이었으며 미세·대혈관 합병증군에서는 10.4±6.4 ng/mL, 484.8±269.7 ng/mL, 388.4±132.4 mg/dL으로 TAT의 증가를 보였으나 역시 연령을 보정한 후에는 통계학적 유의성은 없었다. 4. 미세혈관합병증군에서 HbA1c(>8%)가 높은 군의 PIC 농도가 유의하게 높았고(p=0.049), 대혈관합병증군에서 HbA1c(>8%)가 높은 군의 총 콜레스테롤 농도가 유의하게 높았다(p=0.042). 5. 총 당뇨병 환자군에서 PIC는 fibrinogen과 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며(r=0.47, 0.31,-0.25), 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다(r=0.67). 결론:이상의 결과에서 혈장 TAT 및 PIC 농도는 당뇨병 환자에서 정상 대조군에 비해 의미있게 증가되어 있었고, 당뇨병 환자군에서는 연령의 증가와 유병기간이 혈액응고항진 및 용해의 장애에 큰 역할을 함을 알 수 있었으며, 총 당뇨병 환자군에 PIC와 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다. 따라서 당뇨병 환자에서 혈액응고 및 용해의 장애가 동반되어 있다고 볼 수 있으며, 혈장 TAT 및 PIC는 혈관합병증으로의 진행을 예측하는 지표로서 유용하리라 생각된다. 또 혈당조절정도와 상관성이 있으므로 혈당조절후에 추적검사를 시행하여 합병증의 예방이 가능한지 추후 연구가 필요하리라 생각된다. Background : Abnormality of coagulation and fibrinolystic system is known as a predisposing factor of vascular complication in diabetes. Although the pathogenesis is not well known, non-enzymatic glycation reaction and the increase in production of free radicals due to an increased oxidative stress may be linked to the hypercoagulibility and hypofibrinolytic activity. As indices of abnormality in coagulation and firinolysis in peripheral blood, plasma thrombin-antithrombin Ⅲ complex (TAT) and plasmin-α_2-plasmin inhibitor complex (PIC) were measured. The purpose of this study was to clarify whether hypercoagulability exists in diabetic patients with or without vascular complication. Methods : In our study, we measured plasma thrombin-antithrombin Ⅲ compelx (TAT) and plasmin-α_2-plasmin inhibit or complex (PIC) in 101 diabetic subjects and 20 controls. Comparing TAT and PIC levels in diabetic microvascular complication group, diabetic macrovascular complication group and controls, we examined correlation between risk factors associated with diabetic vascular complication. Results : 1. The group with diabetic vascular complication was older than group without complication. There was no significant difference in BMI, blood pressure, HbA_ic, blood sugar level, insulin, C-peptide, serum creatinine, total cholesterol, triglyceride, HDL-cholesterol, Lp (a) between two groups. The group with diabetic microvascular complication had longer duration of diabetes. 2. Concentration of TAT and PIC were 2.8±1.2 ng/ mL, 240.4±69.7 ng/ mL in controls and 9.5±22.6 ng/ mL, 472.2±258.7 ng/ mL in diabetic patients, respectively. TAT and PIC were significantly higher in diabetic patients than in control (p<0.001). But TAT/PIC ratio was no significant difference between two groups. 3. In diabetic patients, concentration of TAT and PIC and fibrinogen were respectively 4.1±2.4 ng/ mL, 362.2±272.0 ng/ mL, 322.7±102.4 mg/ dL in group without vascular complication and 5.3±4.1 ng/ mL, 529.5±258.7 ng/ mL, 374.9±106.2 mg/ dL in group with microvascular complication, which group had increase in PIC and Fibrinogen but no significance after correction of age. Concentration of TAT and PIC and Fibrinogen were 60.±4.9 ng/ mL, 507.4±321.6 ng/ mL, 427.1±194.7 mg/ dL in macrovascular complication, and 10.4±6.7 mg/ mL, 484.8±269.7 ng/ mL, 388.4±132.4 mg/ dL in combined vascular complication which group showed increase of TAT but also had no significant increase after correction of age. 4. In diabetic microvascular complication patients, group of high HbA_1c (>8%) (p=0.049) had significant high PIC concentration. In diabetic macrovascular complication patients, group of high HbA_1c (>8%) (p=0.042) had significant high total cholesterol concentration. 5. In all diabetic patients, PIC was positively correlated with fibrinogen and HbA_1c and negatively correlated BMI (r=0.47, 0.31, -0.25). Only in daibetic patients without angiopathy, TAT was positively correlated with HbA_1c (r=0.67). Conclusion : In this study, plasma TAT and PIC concentration significantly increased in diabetic patients compared with controls, and PIC was increased in group with microvascular complication, TAT were increased in group with combined micro macrovascular complication. However, there was no significance relationship existed when correctinf for age. PIC was correlated with HbA_1c. TAT was correlated with HbA_1c only in the group without angiopathy. Abnormality of coagulation and fibrinolysis were combined in diabetes, plasma TAT and PIC can be used as an index of vascular complication. Also we found the correlation with the degree of the blood glucose control. Therefore we need follow up study for the possibility of prevention of vascular complication after controlling the blood glucose to age-matched patients (J Kor Diaabetes Asso 25:354~363, 2001).

Cd(Ⅱ) 폴라로그람의 異常波에 關한 硏究

崔七男,朴聖珪,丁梧鎭,문경국 조선대학교 기초과학연구소 1984 自然科學硏究 Vol.7 No.1

pH 6.0∼12.0 範圍에서 0.15M 타르타르 酸鹽支持溶液 속에서 Cd(Ⅱ) Polarogram을 25℃에서 二段還元波를 調査한 결과 pH8.2部分 까지는 限界電流 및 半波電位값이 一定하며 한편 같은 濃度의 窒酸鹽 支持溶液을 사용하였을 때에 比하여 一段還元波의 半波電位값은 0.03volt 陰의 電位값으로 移動하고, 限界電流 값은 21% 減少되었다. pH8.2 部分 以上부터 限界電流 값이 急激하게 增加하다가 pH8.9 以上부터는 다시 急激하게 減少하는 現象을 보여 주었다. 한편 pH8.2 以上부터는 半波電位가 pH11.0에서는 -1.37volt로 되었다. 可能한 電極反應을 假定하여, pH11.0에 이르기까지의 還元波移動의 原因을 檢討하였다. The second reduction waves of the polarogram of Cd(Ⅱ) in 0.15M sodium tartrate solution were investigated in the range of pH from 6.0 to 12.0 at 25 C. Up to pH 8.2, the limiting current and the half wave potential were found to be constant, and when compared with the nitrate solution equal to the concentration of sodium tartrate, the limiting current of the first reduction wave has been decrease 21% and the half wave potential of the first reduction wave shifted by -0.03 volt. The values of limiting current were sharply increased above pH 8.2, and maximum value was obtained at pH 9.8. The half wave potentials were gradually proceeded in negative direction with increasing pH and the half wave potential of -1.37 volt was obtained at the pH 11.0. Suggesting possible mechanisms of electrode reactions, the anomalous behavior of reduction waves of Cd(Ⅱ) in sodium tartrate solution up to pH 11.0 was discussed.