Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

연극 안의 배우, 배우의 몸 - 이경성과 크리에이티브 바키(Creative VaQi)의 연극 미학

이경미 ( Lee Kyung Mi ) 한국연극학회 2017 한국연극학 Vol.1 No.61

이경성과 크리에이티브 바키(Creative VaQi)의 연극은 희곡의 재현이 아니라 `공간`에 대한 질문에서 출발한다. 광화문이나 문화역 서울과 같은 일상적 공간 속에서 행해졌던 그의 작업은 이들 공간으로부터 고유의 장소성, 즉 그 공간에 축적된 역사적, 사회적 맥락을 드러냈다. 이후 그들은 일상적 공간으로부터 극장이라는 제도적 공간으로 중심을 옮겨 극장의 장소성에 대해 근본적인 질문을 던지는데, 이것은 연극이 무엇인가에 대한 자기질문이기도 하다. 이로써 이경성과 바키의 연극은 예의 그 `장소특정적 공연`의 카테고리를 벗어나 보다 더 미학적 지평을 확장해가고 있다. 그 과정에서 우리는 그들의 작업을 공간과 함께, 좀더 시각을 달리하여 공간을 점유한 몸의 문제를 근거로 새롭게 논의할 수 있는 단초를 얻게 된다. 사실 공간에 대한 질문에서 출발한 바키의 작업은 애초부터 그 공간을 점유하고 있는 배우의 몸을 배제하고는 논의할 수가 없는 것이었다. 본 논문은 크리에이티브 바키의 연극을 배우, 배우의 몸으로부터 새롭게 논의하고자 한다. 통상적 연극과 다르게 그들은 연출과 배우 모두가 직접 공연 전 과정을 이끌면서 관련 자료를 조사하고 인터뷰하며, 여러 번의 발제와 토론 등의 과정을 거쳐 공연의 틀을 잡아간다. 이런 작업과정에서 배우는 어떻게 대상을 체험하는지, 그리고 체화된 감각을 바탕으로 무대를 그들 연극의 주요 화두인 공간과 시간, 더 나아가 타자에 대한 감각의 장소로 발생시키는지를 살펴보고자 한다. 무대 위의 배우의 몸은 공간을 점유한 몸으로서 `여기`에 대해 질문하기도 하고, 시간을 점유하고 `지금`에 대해 묻는다. 그런가하면 보이지 않는 타자의 고통을 감각하는 몸으로서, 기억에 대해 묻기도 한다. 배우의 몸에 기반한 이경성의 연출미학을 풀어내는 이론적 근거는 몸과 지각의 문제를 중심에 둔 현상학에서 가져왔으며, 구체적인 사례들은 최근 2년 동안 발표된 이들의 작품들에서 찾아보았다. Lee Kyung-Sung and the theatergroup `Creative VaQi) have made their theaters focusing on space discourses. But for about last two years, most of their works have been performed not only in everyday spaces but also in various instituional theaterspaces. This is why the research about their theater aesthetics should be conducted from different perspectives. This study examines several recent works that Lee Kyung Sung and his VaQi have performed in institutional theatrical spaces. Actor`s bodies that occupy the space have been their theatrical interest that they have focused on from the beginning. What they emphasize is not how they make actors represent their drama roles. They see actors not as tools of theater but as productive subjects. Actors in their theaters stand on stage as themselves with their own identities. In other words, they reveal themselves on stages as persons who have their own thoughts about the world. This is due to their unique working style that are completed through thorough collaborative work, from data research to interviews, presentations and discussions. Of course, actors are the center of all these processes. They constantly interact with objects and build their own viewpoint on them. The body itself is a performing body that creates events and forms the framework of perception and experience. This way of working is closely linked to the phenomenological point of view. Based on this phenomenological point of view, this paper examines how actor`s body is posing new questions about space and time. It tries also to look at how actor`s body occurs as a place of sensation for the other.

DEVELOPMENT OF HIGH VALUE-ADDED TEXTILE PRODUCTS WITHIN THE GLOBALIZATION OF THE KOREAN WAVE

Mi Suk Lee,Kyung Hee Chung,Song Mi Lim,Qingqing Luo 글로벌지식마케팅경영학회 2015 Global Fashion Management Conference Vol.2015 No.06

Fresh attention is being paid to lifestyle brands offering differentiated contents and value such that the 21st century is now being called the age of global lifestyle. Recently national income has been increasing and a broad lifestyle culture has been established. Thus, brands with Scandinavian (Sweden, Denmark, or Norway), culture sensitivity, and design have deeply permeated the domestic market which has led to a domestic consumers' lifestyle trend (Chung& Park, 2004; Kwon, 2013; Magnus & Chrystin, 2003). In particular, such global lifestyle brands as Marimekko (Finland), CathKidston (UK), and Muji (Japan) have secured competitiveness in the global market as textile products which reflect its country's cultural identity (Lee& Park, 2014). Meanwhile, a new word, Hanban (韓版)which refers to Korean brands or products that were created from the Korean wave (韓流), which refers to Korean dramas or songs. Korean culture has been commercialized and grafted into various industries. In particular, a strong wind of Hanban in fashion cultural products not only has spread the Korean lifestyle to the world, but has enhanced its influence on related industries as well as fashion. Although certain major Korean companies launched lifestyle brands such as Jaju, Modern House, Butter, and Pum to help realize a Korean lifestyle brand business, most products sold in Korean lifestyle brands focus on overseas imported goods,so it is difficult to find Korean images on these products(Bang, 2004; Lee & Chung, 2013). The size of the lifestyle market is growing as a high value-added industry that can lead consumers' lifestyle trends, and there is a growing interest for Korean fashion cultural products. Therefore, the purpose of this study is to attempt to develop textile designs for Korean-made high value-added products with the improvement of Korean brand values. For study methodology, the status of lifestyle brands at home and abroad was examined and textile designs were developed for lifestyle brand B. For the development of the textile designs, demand required by brand B was researched and a textile design concept was established based on fashion and interior decoration trends. Then, a textile was designed using a motive that can effectively represent the identity of brand B and the Korean image. Adobe Photoshop 6.0 and Adobe Illustrator CC programs were used for the standardized textile design motif, pattern development, and colorway. The results were as follows. First, global lifestyle brands such as Marimekko, CathKidston, and Muji sell differentiated lifestyle products from other brands by applying motifs and colors which symbolize their country's and city's cultural image on textile products and displaying their own identity and peculiarity. On the other hand, Korean lifestyle brands such as Casamia, Hanssem, Kosney, and Art Box do not plan or produce their own design, but will import and sell foreign textile fabric or textile products with high recognition, or copy imported fabric. Thus, their textile design is unsatisfactory to express their own identity and satisfy consumers' needs. Second, as to the development direction required by brand B, a Korean lifestyle brand, the focus was to first, 'design American and European products using colors and techniques which reflect Korean image trends', Second, to create a 'design with Scandinavia's peculiar simplified line and sensitive color', and finally, to make a 'flower pattern design with good sustainable market feasibility'. Based on the direction and 2015 S/S color and textile trends, three concepts were established: Global Tribe, Bunny in the Wonderland, and Beyond Nature. Global Tribe is a folk paintin g(Minhwa) representing the Korean image well, and was reinterpreted in a modern sense. Bunny in the Wonderland is a modern Scandinavian style design using a rabbit, a symbol of brand B, as the main motif. Beyond Nature used a flower motif in various expression techniques and images. A textile design was developed by extracting from a folk painting, a rabbit, and a flower motif and standardizing a motif in watercolor, pen, and graphic techniques. It was digitalized by a scanner and a pattern was made via the steps of color adjustment and repetition. Three kinds of textile designs were developed according to three concepts and four kinds of colorways were made for each design. This study attempted to develop a textile design as Korean-made high value-added textile products. It is meaningful to suggest textile design with Korean cultural images and brand identity. Further study will focus on the development of fashion cultural products and DIY products to commercialize developed textile design.

‘문명화’와 ‘동화’ 사이에서 주체되기: 근대 동아시아에서 르봉 수용과 이광수의 민족개조론

이경미(LEE Kyung Mi) 한국국제정치학회 2021 國際政治論叢 Vol.61 No.1

이 글의 목적은 이광수의 ‘민족개조론’이 귀스타브 르봉(Gustave Le Bon)의 ‘민족심리학’을 어떻게 수용했는지 살펴봄으로써 그의 논리체계가 갖는 독창적인 함의를 밝히는 데 있다. 식민지 조선의 사상을 알기 위해서는 그를 둘러싼 ‘복합적인 연쇄구도’를 알아야 한다. 이를 위해 2장에서는 우선 르봉의 이론이 근대 동아시아에 전파된 과정을 살펴봤다. 특히 일본을 중심으로 번역이 이루어졌지만 그 양상은 복합적이었다는 점, 그리고 근대 동아시아에 있어 르봉의 수용은 ‘내셔널리즘’과 결부된 특징을 가지고 있었다는 점을 지적했다. 다음으로 3장에서는 민족개조론의 르봉 수용이 갖는 독창성을 일본에서 수용된 상황과 비교를 통해 밝혀냈다. 이광수는 민족성을 ‘근본적인 것’과 ‘부속적인 것’으로 나눈 르봉의 구분을 전유함으로써, 비서구사회의 ‘문명화’를 부정하는 르봉의 이론을 반박하는 동시에 이민족의 ‘동화’를 비판하는 르봉의 주장을 그대로 살려냈다. 즉 민족성의 변화를 ‘긍정’하는 동시에 ‘부정’하는 ‘모순적인 과제’를 하나의 텍스트 안에서 수행했던 것이다. 이는 ‘문명화’와 ‘동화’라는 상반된 과제를 ‘공존’시키는 언설행위였지만, 양자 사이에서 민족의 ‘주체화’라는 공통된 과제를 수행한 것이기도 했다. 그런 의미에서 본고는 이광수의 르봉 수용이라는 문제를 ‘내셔널리즘’이라는 프리즘으로 재조명했다. This paper examines the process how Lee Kwangsu accepted Gustave Le Bon’s theory in colonial Korea. He translated part of Le Bon’s book, The Psychology of People and appropriate his theory to apply for his colonial situation. In order to understand intellectuals in colonial Korea, we must know the “complex chain of thoughts” surrounding them. Therefore, I first examined how Le Bon’s theory was spread to modern East Asia, especially Japan, China and Korea. Through this process, I tried to reveal not only the complex structure but also the linkage between Le Bon’s theory and nationalism in modern East Asia. Second, I described how Lee Kwangsu’s On the National Reconstruction (Minjok Gaejoron) accepted Le Bon’s theory. Comparing with the situation in Japan, he made a different conclusion from Le Bon, using the distinction of nationality as “fundamental” and “adjunctive”. In other words, unlike Le Bon’s interpretation to emphasize the invariability of the fundamental nationality, he argued that the adjunctive nationality is easy to change. His appropriation of Le Bon’s logic was to transform his conclusion to deny the “civilization” of the non-European nation. At the same time, however, Lee inherited Le Bon’ criticism against the “assimilation” of nation. In this respect, Lee’s article had a “originality”, that was not seen in Japan, as a nationalistic discourse in colonial situations. He aimed to establish Korean nation as a “subject or Juche” in it’s contradictory task between “civilization” and “assimilation”.

강남경희한방병원에 내원한 냉증을 호소하는 여성의 Heart Rate Variability 특성 연구

이미주 ( Mi Joo Lee ),김은경 ( Eun Kyung Kim ),이진무 ( Jin Moo Lee ),조정훈 ( Jung Hoon Cho ),장준복 ( Jun Bock Jang ),이경섭 ( Kyung Sub Lee ),이창훈 ( Chang Hoon Lee ) 대한한방부인과학회 2011 大韓韓方婦人科學會誌 Vol.24 No.3

An S-locus receptor-like kinase in plasma membrane interacts with calmodulin in <i>Arabidopsis</i>

Kim, Ho Soo,Jung, Mi Soon,Lee, Kyunghee,Kim, Kyung Eun,Yoo, Jae Hyuk,Kim, Min Chul,Kim, Doh Hoon,Cho, Moo Je,Chung, Woo Sik Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca<SUP>2+</SUP>-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.</P><P><B>Structured summary</B></P><P>MINT-6800947:<I>CBRLK1</I> (uniprotkb:Q9ZT06) and <I>AtCaM2</I> (uniprotkb:P25069) <I>bind</I> (MI:0407) by <I>electrophoretic mobility shift assay</I> (MI:0413)</P><P>MINT-6800966:<I>AtCaM2</I> (uniprotkb:P25069) and <I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>bind</I> (MI:0407) by <I>competition binding</I> (MI:0405)</P><P>MINT-6800930:<I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>binds</I> (MI:0407) to <I>AtCaM2</I> (uniprotkb:P25069) by <I>far Western blotting</I> (MI:0047)</P><P>MINT-6800978:<I>AtCaM2</I> (uniprotkb:P25069) <I>physically interacts</I> (MI:0218) with <I>CBRLK1</I> (uniprotkb:Q9ZT06) by <I>cytoplasmic complementation assay</I> (MI:0228)</P>

대학생의 노인에 대한 태도에 관한 연구

이영희,권미경,김남선,김혜원,최미경,이규은,홍윤미 關東大學校 醫科大學 醫科學硏究所 2003 關東醫大學術誌 Vol.7 No.1

The purpose of this study is to identify the perception, the future and images, and prejudice toward old people. And to examine difference the group of gerontological education (educated group) with the non-educated group. 189 students in K university were participated in this study. The educated group was composed 101 students who enrolled liberal arts course that learned this title. Data were collected from May to June, 2002. The results are as follows; 1) Many students perceived the elderly that was started 60~64 years. 2) The perception, the future and images, and prejudice of both groups were some positively. 3) Each variables were not significantly difference. But the mean level of the educated group were more higher. 4) The educated group was found significantly related to the perception and prejudice(r=.303, p=.002). The non-educated group was found significantly related to the perception and the future and image(r=.421, p=.000), the perception and prejudice(r=.321, p=.003), the future, image and prejudice(r=.272, p=.012).

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

Alkylhalide가 Mouse의 LDH isozyme 분포에 미치는 영향 : Cellulose Acetate Electrophoresis Method

권미경,김영옥,박은주,이동화,이명미,이문희,이양자 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The effect of Carbontetrachloride administration on Lactatedehydrogenase isozyme patterns were analyzed by cellulose acetate electrophoresis for the brain, heart, kidney, lung, liver and mouse muscle. Each other tissue was found to have a characteristic distribution of these isozymes. At normal condition, aerobic tissue such as brain, heart, kidney, and lung were found to have all five isozymes from LDH_1 to LDH_5, while anaerobic tissues such as liver and muscle were found to have superiorly LDH_5 The toxicity of Carbontetrachloride administration was most prominence in the liver, and lung toxicity was occured also.