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Highly Sensitive Biological Analysis Using Optical Microfluidic Sensor
Lee, Sang-Yeop,Chen, Ling-Xin,Choo, Jae-Bum,Lee, Eun-Kyu,Lee, Sang-Hoon Optical Society of Korea 2006 Current Optics and Photonics Vol.10 No.3
Lab-on-a-chip technology is attracting great interest because the miniaturization of reaction systems offers practical advantages over classical bench-top chemical systems. Rapid mixing of the fluids flowing through a microchannel is very important for various applications of microfluidic systems. In addition, highly sensitive on-chip detection techniques are essential for the in situ monitoring of chemical reactions because the detection volume in a channel is extremely small. Recently, a confocal surface enhanced Raman spectroscopic (SERS) technique, for the highly sensitive biological analysis in a microfluidic sensor, has been developed in our research group. Here, a highly precise quantitative measurement can be obtained if continuous flow and homogeneous mixing condition between analytes and silver nano-colloids are maintained. Recently, we also reported a new analytical method of DNA hybridization involving a PDMS microfluidic sensor using fluorescence energy transfer (FRET). This method overcomes many of the drawbacks of microarray chips, such as long hybridization times and inconvenient immobilization procedures. In this paper, our recent applications of the confocal Raman/fluorescence microscopic technology to a highly sensitive lab-on-a-chip detection will be reviewed.
Lee, Won-Kyu,Lee, Sang-Yeop,Na, Jung-Hyun,Jang, Sung-Woo,Park, Chan-Ryang,Kim, Soo-Youl,Lee, Si-Hyeong,Han, Kyou-Hoon,Yu, Yeon-Gyu Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.6
Nopp140 is a highly phosphorylated protein that resides in the nucleolus of mammalian cell and is involved in the biogenesis of the nucleolus. It interacts with a variety of proteins related to the synthesis and assembly of the ribosome. It also can bind to a ubiquitous protein kinase CK2 that mediates cell growth and prevents apoptosis. We found that Nopp140 is an intrinsically unfolded protein (IUP) lacking stable secondary structures over its entire sequence of 709 residues. We discovered that mitoxantrone, an anticancer agent, was able to enhance the interaction between Nopp140 and CK2 and maintain suppressed activity of CK2. Surface plasma resonance studies on different domains of Nopp140 show that the C-terminal region of Nopp140 is responsible for binding with mitoxantrone. Our results present an interesting example where a small chemical compound binds to an intrinsically unfolded protein (IUP) and enhances protein-protein interactions.
골수이형성 증후군 환자에서 발생한 조직구양 Sweet 증후군 1예
이규엽 ( Kyu Yeop Lee ),권연숙 ( Yeon Sook Kwon ),김도영 ( Do Young Kim ),김세훈 ( Se Hoon Kim ),이광길 ( Kwang Gil Lee ),방동식 ( Dong Sik Bang ) 대한피부과학회 2007 大韓皮膚科學會誌 Vol.45 No.7
Sweet`s syndrome is an acute febrile neutrophilic dermatosis that occurs with malignant disease, mainly myeloid hemopathies, in about 20% of cases. When associated with myelodysplasia, clinical and histologic features of Sweet`s syndrome can be atypical; however its significance is still debated. We encountered a case of Sweet`s syndrome associated with myelodysplastic syndrome in a 63-year-old woman. The clinical presentation strongly suggested Sweet`s syndrome but, histopathologic examination showed dense histiocytic infiltration with prominent upper dermal edema and little neutrophil infiltration. In the evaluation for atypical histopathology and laboratory abnormalities, she was diagnosed with myleodysplastic syndrome by peripheral blood smear and bone marrow biopsy. Histiocytoid Sweet`s syndrome is a currently accepted concept and probably has a close relation with underlying myeloid hemopathies. (Korean J Dermatol 2007;45(7):754∼757)
모스미세도식수술로 치료한 소낭성 피부 부속기 암종 1예
이규엽 ( Kyu Yeop Lee ),구본철 ( Bon Cheol Goo ),장재용 ( Jae Yong Jang ),정기양 ( Kee Yang Chung ) 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.12
Microcystic adnexal carcinoma (MAC) is an uncommon skin appendage neoplasm with both follicular and sweat duct differentiation. It usually extends microscopically far beyond assessed clinical margins and spreads locally in the dermal, subcutaneous fat, muscle, and perineural tissue planes. Although its metastasis is very rare, local recurrence rate after standard excision is 40~59%. Recently, Mohs micrographic surgery (MMS) was introduced for the treatment of MAC and a more favorable cure rate has been reported. We report a case of MAC, which was treated by MMS, which concurred with thymoma and thyroid papillary carcinoma in a 50-year-old woman. (Korean J Dermatol 2006;44(12):1444~1447)