腎絲球體疾患의 病理學的 및 免疫病理學的 檢索 : 第一篇 腎症候群 Part 1, Nephrotic Syndrome

徐光善 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

An analysis for 76 cases of nephrotic syndrome has been carried out from a series of 179 renal biopsy specimens which were examined by light microscopic and immunofluorescent studies at Department of Pathology, College of Medicine, Chungnam National University, during a period of 39 months from August 1983 to October 1986. The results were as follows; 1. A total of 179 kidney needle biopsies were composed of 150 cases (83.8%) of the primary glomerulonephritis(GN) and 29 cases(16.2%) of systemic diseases affecting the glomerulus. Among the primary GN, the most frequent disease entity was IgA nephropathy 61 cases, followed by membranous nephropathy 27 cases, lipoid nephrosis 16 cases, etc. Among the systemic diseases, the lupus nephritis was the most common, i.e. 11 cases. The following were hepatitis B-associated GN 8 cases, Henoch-Schёnlein purpura 4 cases, etc. The age distribution was under 16 years 12.8% and under 30 years 62.6%. The male to female ratio was 1.5:1. 2. In 76 cases(42.5%) of renal biopsy specimens the clinical impression was nephrotic syndrome. There were composed of 66 cases(86.8%) of primary GN and 10 cases(13.2%) of systemic diseases. The age distribution was under 16 years 7.9% and 16-29 years 52.6%. The male to female ratio was 2:1. In children all cases(6 cases) were due to primary GN. In adult primary GN was 60 cases(85.7%), which were composed of membranous nephropathy 26 cases, lipoid nephrosis 12 cases, focal segmental glomerulosclerosis 10 cases, etc. Systemic diseases was 10 cases(14.3%), which were composed of hepatitis-B associated GN 5 cases, lupus nephritis 2 cases, etc. 3. The immunofluroescent studies were negative in most cases of lipoid nephrosis and focal segmental glomerulosclerosis, except few cases demonstrating weak mesangial reactions of IgM. Membranous nephropathy illustrated uniform fine granular staining largely of IgG(65.4%) and C_3(50%), along the glomerular basement membranes. Membranoproliferative GN demonstrated a peripheral lobular granular deposit predominantly of IgG(85.7%) and C_3(71.4%). Lupus nephritis showed granular, weak mesangial deposits of C_3 in one case. The other one exhibited granular mesangial, as well as loop, deposits of IgG, IgA, IgM, C_3, and fibrinogen. Syphilitic nephritis showed uniform fine granular staining along the glomeular basement membranes for IgG, C_3 and fibrinogen. Hepatitis-B associated GN illustrated granular mesangial and loop deposits of C_3(100%), IgM(100%), and IgA(40%).

腎絲球體疾患의 病理學的 및 免疫炳理學的 檢索 : 第二篇 狼瘡性 腎炎 Part 2. Lupus Nephritis

徐光善 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.1

A clinicopathologic analysis for 16 cases of lupus nephritis has been carried out from a series of 348 renal biopsy specimens which were studied at Department of Pathology, College of Medicine, Chungnam National University, during a period of 56 months from October 1983 to May 1988. The results were as follows; 1. Lupus nephritis was 4.6% of renal biopsy specimens and 43.8% of nephropathy due to systemic diseases. It was common in young adults and 43.8% of patients were in the fourth decade. Male to female ratio was 1 : 15. 2. WHO classification was adopted for the histologic grading of glomerular lesions, as follows; mesangial proliferative glomerulonphritis (Class II) 6. 3%, focal proliferative glomerulonephritis (III) 6.3%, diffuse proliferative glomerulonephritis (IV) 75.0%, and membranous glomerulonephritis (V) 12.5%. Mean of chronicity and activity indexes of WHO class IV was 1.8 and 6.6, respectively. There was some trend that chronicity index was increased along with activity index. 3. The immunofluorescence microscopic findings were as follows; The granular deposits of IgA were observed in 87. 5%, C₃87.5%, IgG 81. 3%, IgM 81.3%, and fibrinogen 37. 5%. 4. Mean duration of disease until kidney biopsy has been performed 'was 11.7 months. Among the clinical findings, facial rash was observed in 43.8% of cases, arthritis 37.5%, and dyspnea 37.5%. On serologic study, antinuclear antibody was positive in 61.5%, LE cell 50.0%, and anti-DNA antibody 54.5%. The complement 3 and complement 4 were decreased in all cases. On urinalysis, hematuria and proteinuria were observed in 87.5% of 'cases. In order to study about the prognostic factors of lupus nephritis, a long duration of follow-up including repeat biopsy is necessary.

Waldeyer's ring과 비강에 발생한 다형성 세망증과 비호지킨씨 림파종에 대한 면역병리학적 및 임상적 연구 : Immunopathologic and Clinical Studies

서광선 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

The Waldeyer's ring is frequently involved with non-Hodgkin's lymphoma(NHL); however, the immunologic aspect of Waldeyer's ring lymphoma(W-NHL) has been poorly investigated, as have the clinical and immunologic aspects of extranodal lymphomas arising from the nasal cavity or paranasal sinus (N-NHL). In order to demonstrate differences between the immunologic and clinical features of W-NHL and N-NHL, including polymorphic reticulosis (PR), twenty-four cases of WNHL, and N-NHL were studied for immunophenotype, using UCHL-1 and MB-2 monoclonal antibodies, and histopathologic correlation with clinical features. The results of this study are as follows: In nine cases of W-NHL, six (66.7%) had B-cell markers and three (33.3%) T-cell markers, whereas all of fifteen cases(100.0%) of N-NHL had T-cell markers. Two cases of N-NHL and seven cases of N-NHL showed the characteristic histopathological features of PR. All of nine cases of PR had T-cell phenotype and were classified as a peripheral T-cell lymphoma(PTL). As a whole, eighteen cases(75.0%) showed T-cell phenotype; nine cases PR, five cases "diffuse, mixed cell" type, and four cases "diffuse, large cell" type, and six cases (25.0%) B-cell phenotype; three cases "diffuse, large cell" type, two cases "diffuse, mixed cell" type, and one case "diffuse, small-cleaved cell" type. The survival rate of the T-cell lymphoma group was inferior to that for the B-cell lymphoma group.

切除된 甲狀腺組織의 針生檢에 依한 病理組織學的 硏究

徐光善,姜大榮 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.1

In order to reevaluate the accuracy of needle biopsy compared with permanent section except technical error, 163 subtotally or partially resected thyroid specimens were studied, which have been submitted to department of pathology, Churgnam National University School of Medicine, during the period of 20 months from January 1982 to August 1983. The results were as follows: 1) In 163 cases observed, 97 cases (59. 5%) goiter, 37 cases (22.7%) carcinoma, 19 cases X11. %) adenoma, and 10 cases (6. 1%) thyroiditis were included. 2) Among 163 cases, 148 cases were female and the ratio of- male and female was 1 : 9.9. The average age of goiter was 33.4 year-old, carcinoma 37.6, adenoma 35.2, and thyroiditis 37. 3) On cross section of the thyroid tissue, the average diameter of the nodules were 3. 1cm in goiter and carcinoma, and 3. 5cm in adenoma. 4) Nontoxic goiter comprised about 95. 8% of all goiters. Nontoxic goiter was 86.6% of all goiters. 5) Papillary carcinoma was 62. 2% of all carcinoma. Among papillary carcinoma (23 cases), psammoma bodies were observed in 12cases (52.2%'). 6) The result of needle biopsy diagnosis in goiter and thyroiditis were compatible with permanent section diagnosis (100%). But, slight difference was noted in the adenoma and carcinoma, according to the biopsy site. In the follicular carcinoma, the accuracy of needle biopsy dignosis was 71.4% in central portion and 85.7% in margin of the nodule.

Dimethylbenzanthracene과 N-Nitroso-N-Methylurea 投與에 依한 腫瘍發生에 미치는 Caffeine의 影響에 關한 實驗的 硏究

徐光善,申石澈 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

An experimental study was carried out to observe the effects of caffeine on Dimethylbenzanthracene(DMBA)-and N-Nitroso-N-Methylurea(NMU)-induced carcinogenesis in Sprague-Dawley rats. A total of 120 Sprague-Dawley rats were divided into 9 groups and the well-studied chemical carcinogen, NMU, was given to female Sprague-Dawley rats, with and without DMBA administration. These rats were fed with placebo or two doses of caffeine-supplemented drinking water and observed for the incidence of cancers. At the 32th week, all the palpable tumors observed were removed for histologic confirmation. The results were as follows: 1. There was significant increase in incidence of cancers in DMBA combined with NMU-treated, groups (70. 8%) compared with NMU-treated groups (33.3%, P<0.01). The groups treated with DMBA and NMU had a highest mortality rate over the period of study (P<0.01). 2. The incidence of cancers was 52. 5% in the zero-dose caffeine groups, 37. 5% in the low-dose caffeine groups (lmg/ml), and 35. 0% in the high-dose caffeine groups (2mg/ml). On the comparison between proportions, this difference was not statistically significant (P>0.05). But, the relative risk of caffeine on carcinogenesis was 0. 69. So, caffeine was proved to have some protective effect on the formation of cancers. The high-dose caffeine increased the animal mortality (P<O. 05). 3. During the period of study, the body weight of rats was steadily increased. At the 16th week, the rats that received DMBA combined with NMU weighed significantly less than the animals that did not receive carcinogen(P<O.05). At the 32th week, this statistical difference was not significant (P>O. 1). The presence and dosage of caffeine did not significantly change animal weight(P>0.05). 4. Various types of cancers were observed. The most common malignant tumor was breast cancer 40 cases, followed by squamous cell carcinoma 27 cases, sarcoma 6 cases, malignant lymphoma 4 cases, etc. The total number of cancers was highest in the zero-dose caffeine groups. The incidence of breast cancer was not significantly different between DMBA combined with NMU-treated groups and NMU-treated groups (P>0.05). But, the incidence of squamous cell carcinoma was higher in the DMBA combined with NMU-treated groups (P<0.01). By the relative risk, caffeine was proved to have some protective effect on the formation of cancers in the breast and skin. On the comparison between proportions of the zero-dose caffeine groups and the high -dose caffeine groups, this difference was statistically significant (P<0. 01). The presence or amount of caffeine did not cause detectable histologic differences in the each type of cancers. In conclusion, the present data demonstrated that caffeine was proved to have some protective effect on the DMBA and/or NMU induced carcinogenesis in Sprague-Dawley rats.

위장관 암종에서의 Class Ⅰ 조직적합항원의 발현에 대한 면역조직화학적 연구

서광선,윤완희 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Expression of HLA class I antigens was studied by immunohistochemical staining of acetone-fixed cryostat sections of 6 gastric and 10 colorectal adenocarcinomas using monoclonal antibodies. Immunohistochemical staining for MB-2(pan-B cell marker) and UCHL-1(pan-T cell marker) was also performed on formalin-fixed, paraffin embedded tissues in order to analyse tumor-infiltrating lymphocytes. HLA class I antigens were strongly expressed in normal gastric and colorectal epithelium. In 6 gastric adenocarinomas, which were all poorly differentiated, the expression intensity of HLA class I antigens was mildly decreased, compared to normal epithelium. Among 10 colorectal adenocarcinomas, one mucinous carcinoma was completely negative. The remaining were positive, although the expression intensity was somewhat decreased in moderately differentiated adenocarcinomas. The mononuclear infiltrate in the gastric and colorectal carcinomas consisted mainly of T-cells, although the infiltrate was relatively sparse and sometimes absent. There was no apparent correlation between the degree of differentiation, the intensity of T-cell infiltrates, and HLA class I antigen expression. However, a relatively poor expression of HLA class I antigens was found in association with a decrease in the degree of histologic differentiation.

B형 간염 바이러스 항원혈증에 동반된 신병증의 면역병리학적 고찰

서광선 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

The clinicopathological characteristics of hepatitis B antigenemia-associated nephropathy were analyzed in renal biopsies obtained from 39 HBsAg-seropositive patients. Twenty(51%) of the matients had nephrotic syndrome, 33(85%) proteinuria, and 20(51%) hematuria. Fourteen(50%) out of 28 patients who had received liver function tests showed increased serum transaminase levels. Liver biopsy was performed in six patients. Results showed chronic active hepatitis in five cases and chronic persistent hepatitis in one case. Based on morphology, 24 cases(62%) were classified as membranous nephropathy(MN), 10 cases(26%) as membranoproliferative glomerulone-phritis(MPGN) type I, 3 cases(8%) as mesangial proliferative GN(MsGN), and 2 cases(5%) as inor change. Ultrastructurally, hepatitis B virus(HBV)-associated MN frequently showed intramembranous, mesangial and small subendothelial deposits in addition to the predominant epimembranous deposits typical of the usual findings of idiopathic MN. HBV-associated MPGN demonstrated subendothelial electron-dense deposits and mesangial interposition characteristic of MPGN, but scattered intramembranous and epimembranous deposits were also present. In addition, most of these cases showed weak IgA deposits. In conclusion, HBV-associated nephropathy is characterized by somewhat diverse histopathologic features similar to lupus nephropathy. Although HBsAg was not demonstrable within the glomeruli, HBV infection seems to have a significant effect in the pathogenesis of MN and MPGN.

여성 생식기 콘딜로마 병변에서의 In Situ Hybridization에 의한 Human Papillomavirus DNA의 검색

서광선,강동욱,이증훈 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

The in situ hybridization technique, using digoxigenin labeled human papillomavirus(HPV) probes was applied to the detection of HPV DNA types 6 and 11. on paraffin sections of 24 condylomatous and malignant lesions of the uterine cervix, vagina, and vulva were used. They consisted of 9 condyloma acuminata, 10 flat condylomatous lesions associated with cervical intraepithelial neoplasia (CIN) and invasive squamous cell carcinoma(SCC), and 5 noncondylomatous lesions. The HPV DNA probes were stained mainly stained in the loilocytotic nuclei of the superficial portion of the epithelium. The underlying normal, dysplastic, and carcinoma cells were negative. Seven(77.8%) out of 9 condyloma acuminata and 4(57.1%) of 7 CIN Ⅰ/Ⅱ cases were positive for HPV 6 or 11. However, 2 cases of CIN Ⅲ and 6 cases of invasive SCC were negative, irrespective of the presence of koilocytosis. Thus, it was concluded that HPV types 6 and 11 are more commonly associated with benign condylomatous and low grade intrapithelial lesions than high grade lesions and SCC.

말초신경계 종양의 병리학적 검색

서광선 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

A total of 180 tumors of the peripheral nerve and neuroectodermal tissue is histologically analyzed. These tumors were collected from Department of Pathology, Chungnam National University, during a period of 12 years from January 1973 to December 1984. After critical review of the diagnosis of tumors and their clinical deta we have obtained following informations. 1. Among 180 tumors in this series there were 90 cases of solitary neurofibroma, 26 cases of neurofibromatosis, 1 case of schwannoma with neurofibromatosis, 6 cases of malignant schwannoma, 2 cases of neuroblastoma, 1 case of ganglioneuroblastoma and 2 cases of ganglioneuroma. 2. Age distribution revealed 5 cases in first decade, 41 cases in second decade, 44 cases in third decade, 28 cases in fourth decade, 27 cases in fifth decade, 23 cases in sixth decade, and 10 cases in seventh decade. 3. There were 88 cases of male and 92 cases of female. The sex ratio was 1:1.1. 4. The size (diameter) of the individual tumor mass was as follows; The 35 cases were smaller than 1.0cm. There were 84 cases in 1.0-2.9cm, 38 cases in 3.0-4.9cm, 9 cases in 5.0-6.9cm. The 14 cases were lager than 7cm. 5. These tumor masses were found most frequently in soft tissue of the extremities(57 cases). The remainder occured in head and neck (36 cases), chest and abdomen (26 cases), back and shoulder (23 cases), spinal canal (7 cases) and nasal cavities (5 cases). The 15 cases of neurofibromatosis were noted in multiple lesions at the skin and subcutaneous tissue.

우리나라 경관기본법의 전개방향 제시 : 일본의 경관법과의 비교를 통하여

서주환,양희승,지광선 경희대학교 디자인연구원 2005 예술· 디자인학연구 Vol.8 No.2

Is realized by basis right that spectacle who is important urea that dominate area whole image and quality of life of local resident becomes van chrysanthemum along with social development and according as citizens' way improves, people use gradually. Much social changes are tried, and can speak as spectacle law enactment that among them, the most representative attempt can control police officer to secure these basis right. Therefore, legislation is purposeful to investigate spectacle basis bill and Japan's comparison of spectacle law, format of law through analysis and contents of our country that is presaged, and present desirable unfolding direction of spectacle fundamental law of our country that is established hereafter through this present in this research. The result is that necessity of spectacle concept thesis is required and real and must present correct spectacle plan bill in real condition. Is differentiated and connection need full equipment of available spectacle law. Need spectacle connection organization's participation. Can make country that is beautiful when Majimakeurourimodu is interested about spectacle and participates actively and there is grace.