화상을 입은 백서에서 식이의 성분이 세균전위에 미친 영향

이광만,이경근,조지현 圓光大學校 醫科學硏究所 1997 圓光醫科學 Vol.13 No.1-2

Bacterial translocation means the passage of viable bacteria from the gastrointestinal tract to normally sterile tissues such as the mesenteric lymph nodes and other internal organs. It is now clear that translocation is increased in bums and in injury including hemorrhagic shock. Total parenteral nutrition and elemental enteral diets are thought to be associated with atrophy of the intestinal mucosa and increased translocation. The purpose of this study was to determine whether or not the composition of nutrients effects on bacterial translocation in normal and burned rats. 54 male Sprague-Dawley rats were randomized to 3 groups of 18 animals each. Group Ⅰ was non-burned control rats, and group Ⅱ and Ⅲ were burned rats. Rats in group Ⅱ and Ⅲ were subjected to contact dermal bum of 10% and 20% each of their body surface area. All 3 groups were re-randomized to 3 subgroups of 6 animals each; subgroup 1 fed rat chow, subgroup 2 fed elemental diet(Energen®) and subgroup 3 fed oral TPN solution. Animals were fed respective diets ad libitum for 2 weeks, and then mesenteric lymph nodes, portal vein blood, and cecal content were aseptically obtained for culture. Bacterial count in mesenteric lymph nodes(MLN) of burned rats was significantly increased than that of non-burned rats(p=0.033). In burned rats, MLN bacterial count was the highest in the rats fed oral TPN (566.1±870.5/g vs. 270.1±382.4/g in the rats fed elemental diet and 164.9±184.7/g in the rats fed chow). Cecal culture in burned rats revealed a statistically significant increase in the cecal anaerobic count in the rats fed oral TPN when compared to the rats fed chow or elemental diet(p=0.003). There was close correlation between MLN bacterial count and cecal aerobes count(r=0.843, p=0.001). In conclusion, the type of nutrients had influence on bacterial translocation in burned animals partly by increasing the cecal bacterial population.

손상정도계수와 외상치를 이용한 외상환자의 분석

송근정,장문준,김성중,조광현,김인병,최성욱,이한식 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

Trauma is one of the most common causes of death. Recently, injury severity score(ISS) is widely used to evaluate and classify the patients with multiple injury. Trauma score(TS) was developed as a means of providing rapid accurate assessment of the injured patient. Authors conducted a review of trauma patients who visited the emergency department of YongDong Severance Hospital from September 1992 to August 1993. ISS and TS were collected from comprehensive initial evaluation. The results were as follows : 1) The subjects were 10.2%(2,666 patients) of total 26,202 emergency patients. 2) Male to female ratio was 2.1 : 1. The age distribution was highest under 10 year(24.9%). The mean ISS was 3.5, and the mean ISS increased with ago. 3. Analysis of injury mechanism showed high incidence of traffic accident(35.3%), blunt trauma 25.7%, fall 24.2%, stabbing 10.7%, and others 4.3% in order. 4. The 8.0% of patients were admitted after initial management and their mean Iss was 12.6 mean TS was 15.5. 5. Among admitted traffic accident patients, the mean ISS of passenger T.A., pedestrian T.A. and driver T.A. were 19.0 ±9.9, 14.4 ±10.6, and 11.3 ±7.7 respectively. 6. Among 42 mortality cases, 25 patients were D.O.A. and 17 were expired. The mean ISS of D.O.A. Patients was 30.0; the mean Iss and mean Iss and mean TS of expired patients were 28.3 and 11.8 respectively. 7. The most common cause of death was head trauma(66.7%). 8. By comparing the ISS and TS of admitted and expired patients, mortality occurred above 16 point of ISS. The mortality increased with increasing ISS, but it was influenced by the trauma site and the severity of the particular injury site. Mortality also occurred below 15 point of TS, the mortality increased inversely with TS, and the mortality with TS, and the mortality was high below 13 point (above 40%).

응급실의 약물중독 환자

송근정,조광현,이한식 大韓應急醫學會 1992 대한응급의학회지 Vol.3 No.1

Any short of medical drug, insecticide or foods sometimes makes harmful situation whether it is beneficial or not. Nowadays, the treatment for drug intoxication started mostly in emergency department. The initial treatment must start as soon as possible. The prognosis is depend upon a kind of drug, exposure time and time of initial treatment. Authors conducted a review of acute poisoned patients seen from April 1983 to July 1992, who visited emergency department of Yong Dong Severance Hospital. The results were as follows, 1. The patients of Drug intoxication account for 0.68% of total emergency department patients. 2. Male to female ratio was 1:1.9 and the age distribution revealed a high incidence in second decade(35.7%) 3. From all the cases of drug intoxication and 67.2% was done on purpose. 4. The drug used were sedatives, CO intoxication, pharmaceuticals, pesticide, in order. 5. Admission rate was 23.8% and mean hospital day 6.0 days. 6. ICU admission rate was 30.0% and mean staying time was 4.7 days.

시각장애자용 문자인지 장치에 관한 연구

허웅,조병서,김진곤,함광근,민홍기 明知大學校 産業技術硏究所 1994 産業技術硏究所論文集 Vol.13 No.-

In this paper, we implemented the hangeul tactile recognition system for the Blind. The system is consist of hardware part and software part.The hardware part is consist of a scanner interface image processing system, a electro tactile output device inter and auser interface. The software system consist of pattern learning routine,image processing and character pattern output routine, file management and operation window routine. The 16 by 16 of return electrode is driven by a electro-tacttile device. The recognition experimention distinguished learning beforefrom learning after,after learning the recognition rate is higher than learning before. As a results of experimentation before and after learning,the later is superior to the former. Therefore,if learning is increased, we can take higher recognition rate.

용접공들의 면역능에 관한 연구

이수일,조봉수,김영욱,고광욱,조원근,김영실,강수용,황인경,조병만,김돈균 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.1

The authors examined human immunity indicators such as CD4, CD8, Natural Killer(NK) cell, IgG, IgA, and IgM, and heavy metals such as Pb, Cr, Cd to evaluate the health hazard of welders. We selected total 126 male subjects, 60 welders who had more than 5 year working history as an exposure group, and 66 office workers and medical college students as a control group. This immunity evaluation approach is the first newly designed study that has never been tried in past studies on welder's heath evaluation. And the results were as follows. 1. Total lymphocyte count of peripheral blood was significantly higher in welder group, 2,615±650 ea/㎕ compared with 2,368±681 ea/㎕ in control group(p>0.05). 2. The proportion of CD4, CD8, NK cell in welder group was 37.5±9.4%, 24.0±8.8%, and 21.0±9.5%, respectively, and 35.5±6.5%, 25.0±6.9%, and 19.6±8.8% in control group. CD4 and NK cell were slightly higher, and CD8 was slightly lower in welder group. but there was no statistical significance between two groups(p≥0.05). 3. IgG, IgA, and IgM in welder group were 15.9㎎/㎗, 2.7㎎/㎗ and 1.5㎎/㎗, respectively, and 15.6㎎/㎗, 2.9㎎/㎗ and 1.7㎎/㎗ in control group. There was no statistical significance between two groups(p≥0.05). 4. Blood lead, cadmium and chromium concentrations were 4.2±1.8㎍/㎗, 0.4±0.38㎍/㎗, 1.2±0.6㎍/㎗ in welder group and 3.2±1.7㎍/㎗, 0.44±0.29㎍/㎗, 1.1±0.6㎍/㎗ in control group, respectively, and these value were within normal ranges.

증기압 멸균 소독에 의한 치과용 석고의 물성변화

김연웅,이용근,김광만,김경남,조리라,정경호 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.1

Gypsum products have potential contamination by infectious human pathogens, which could be transmitted from patient to the gypsum model via impression materials. Therefore disinfection and sterilization of gypsum products are recommended. Steam sterilization: namely autoclaving is widely available in dentistry. The aim of this study was to investigate degradation of mechanical properties for dental gypsum after steam sterilization. The effect of autoclaving on compressive strength, weight loss and dimensional accuracy of specimens of commercial die stone such as Crystal Rock(CR : Maruishi), Die Keen(DK : Columbus Dental), Resin Rock(RR : Whip Mix), Tuff Rock(TR : Talladium) were estimated, and experimental gypsums{(EP) : 0.5wt% accelerator, 0.02wt% retarder, 0.1wt% surface agent, 0.1wt% Poly Vinyl Acetate} were also measured the above properties after autoclaving. For each group, five cylindrical(6㎜ in diameter and 12㎜ long) specimens made with manufactures water:powder ratio. All specimens were dried in oven until reaching the constant mass. After dried, they were autoclaved at 121℃ for 20 minute, dried again. Compressive strength was measured before and after autoclaving using mechanical testing machine at a cross-head speed of 0.5㎜/min. The dimensional accuracy was estimated by length change of specimens with digital caliper before and after autoclaving. For statistical analysis, ANOVA Tukeys Studentized Range(HSD) test and T test were used. Compressive strength and weight were decreased in all samples after autoclaving. There is significant difference between CR, DK and RR, TR in after autoclaving(p<0.05). EP exhibited the highest compressive strength after autoclaving. The weight was significantly decreased in all group except RR after autoclaving(p<0.05). The dimensional accuracy was not significantly increased in all group except CR. As this result, we found that resin-containing dental stone is more appropriate for autoclaving in dentistry.

광조사 강도가 복합레진의 중합반응속도에 미치는 영향에 관한 실시간 체적측정법을 이용한 연구

라성호,이인복,김창근,조병훈,이광원,손호현 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.2

Objectives The aim of this study is to evaluate the effect of light intensity variation on the polymerization rate of composite resin using IB system (the experimental equipment designed by DR. IB Lee) by which real-time volumetric change of composite can be measured. Methods Three commercial composite resins [Z100(Z!), AeliteFil(AF), SureFil(SF)] were photopolymerized with Variable Intensity Polymerizer unit (Bisco, U.S.A.) under the variable light intensity (75/150/225/300/375/450mW^2) during 20 sec. Polymerization shrinkage of samples was detected continuously by IB system during 110 sec and the rate of polymerization shrinkage was obtained by its shrinkage data. Peak time(P.T.) showing the maximum rate of polymerization shrinkage was used to compare the polymerization rate. Results Peak time decreased with increasing light intensity(p<0.05). Maximum rate of polymerization shrinkage increased with increasing light intensity(p<0.05). Statistical analysis revealed a significant positive correlation between peak time and inverse square root of the light intensity (AF:R=0.965, Z1:R=0.974, SF:R=0.927). Statistical analysis revealed a significant negative correlation between the maximum rate of polymerization shrinkage and peak time(AF:R=-0.933, Z1:R=-0.892, SF:R=-0.883), and a significant positive correlation between the maximum rate of polymerization shrinkage and square root of the light intensity(AF:R=0.988, Z1:R=0.974, SF:R=0.946). Discussion and Conclusions The polymerization rate of composite resins used in this study was proportional to the square root of light intensity. Maximum rate of polymerization shrinkage as well as peak time can be used to compare the polymerization rate. Real-time volume method using IB system can be a simple, alternative method to obtain the polymerization rate of composite resins.

유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari

Serial cloning of pigs by somatic cell nuclear transfer: Restoration of phenotypic normality during serial cloning

Cho, Seong-Keun,Kim, Jae-Hwan,Park, Jong-Yi,Choi, Yun-Jung,Bang, Jae-Il,Hwang, Kyu-Chan,Cho, Eun-Jeong,Sohn, Sea-Hwan,Uhm, Sang Jun,Koo, Deog-Bon,Lee, Kyung-Kwang,Kim, Teoan,Kim, Jin-Hoi Wiley-Liss,Inc 2007 Developmental dynamics Vol.236 No.12

<P>Somatic cell nuclear transfer (scNT) is a useful way to create cloned animals. However, scNT clones exhibit high levels of phenotypic instability. This instability may be due to epigenetic reprogramming and/or genomic damage in the donor cells. To test this, we produced transgenic pig fibroblasts harboring the truncated human thrombopoietin (hTPO) gene and used them as donor cells in scNT to produce first-generation (G1) cloned piglets. In this study, 2,818 scNT embryos were transferred to 11 recipients and five G1 piglets were obtained. Among them, a clone had a dimorphic facial appearance with severe hypertelorism and a broad prominent nasal bridge. The other clones looked normal. Second-generation (G2) scNT piglets were then produced using ear cells from a G1 piglet that had an abnormal nose phenotype. We reasoned that, if the phenotypic abnormality of the G1 clone was not present in the G2 and third-generation (G3) clones, or was absent in the G2 clones but reappeared in the G3 clones, the phenotypic instability of the G1 clone could be attributed to faulty epigenetic reprogramming rather than to inherent/accidental genomic damage to the donor cells. Blastocyst rates, cell numbers in blastocyst, pregnancy rates, term placenta weight and ponderal index, and birth weight between G1 and G2 clones did not differ, but were significantly (P < 0.05) lower than control age- and sex-matched piglets. Next, we analyzed global methylation changes during development of the preimplantation embryos reconstructed by donor cells used for the production of G1 and G2 clones and could not find any significant differences in the methylation patterns between G1 and G2 clones. Indeed, we failed to detect the phenotypic abnormality in the G2 and G3 clones. Thus, the phenotypic abnormality of the G1 clone is likely to be due to epigenetic dysregulation. Additional observations then suggested that expression of the hTPO gene in the transgenic clones did not appear to be the cause of the phenotypic abnormality in the G1 clones and that the abnormality was acquired by only a few of the G1 clone's cells during its gestational development. Developmental Dynamics 236:3369–3382, 2007. © 2007 Wiley-Liss, Inc.</P>

Construction of Stably Transformed Bm 5 Cells by Using Autographa californica Nuclear Polyhedrosis Virus IE 0 Gene

Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2

To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells