뱀장어 아기미 울혈증에 관한 연구

이근광,김영길,박성우,최민순 한국어병학회 1994 한국어병학회지 Vol.7 No.2

뱀장어의 울혈증 발병 원인구명을 위하여 서해안 지역 5 개 양만장을 대상으로 아가미 울혈증 발병현황과 수질등 역학조사를 실시하였고, 한편으로는 스트레스(handling 과 수온차) 및 병어에서 분리한 아가미 마쇄액을 건강어의 복강에 주사하여 울혈증 발병률과 CHSE-214 에 접종하여 세포변성을 조사하였다. 조사한 양만장의 아가미 울혈증 발병률은 30-80%로서 사육시의 수온과 선별시의 수온차가 클수록 발병률이 높았으며, 수질환경과 본증의 발병과는 뚜렷한 상관관계가 없었다. 또한 발병률이 높은 양만장의 뱀장어는 Ht, Hb, Tp, Alb, Mg 및 Glu 치등이 낮은 반면 GOT, GPT, Ca 및 Met-Hb 치등은 높게 나타나는 경향을 보였으나, 반드시 아가미 울혈증에 비례하여 증감되지는 않았다. 스트레스(handling 과 수온차)를 가함에 따라 아가미 울혈증이 실험적으로 유발되어졌으며, 발병률은 50-70% 이었다. 이러한 스트레스 조건하의 어류의 혈액학적인 성상은 울혈증 발병빈도에 비례하여 Ht, GOT, GPT, Met-Hb, Alb 및 Glu 치등은 증가되었으나, Tp, Hb 및 Mg 은 감소하는 경향을 보였으며, 온도차가 클수록 cortisol 량이 증가되는 경향을 보였다. 그러나, 병어의 아가미 마쇄액을 복강주사 및 CHSE-214 에 접종하였던 바 각각 울혈증의 발병 및 세포변성이 전혀 관찰되지 않았다. 이러한 결과는 선별시 handling 과 수온의 급변이 혈중 cortisol 을 상승시켜 혈액의 전해질 변화를 초래케하므로써 혈액의 흐름을 완만하게 되고 동정맥계로의 혈액유입량이 증가하게 되어 중심정맥동이 확장되므로써 울혈이 형성되는 것임을 시사한다. In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities (stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit (Ht), haemoglobin (Hb), total protein (Tp), albumin (Alb), glucose (Glu), magnesium (Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase (GPT), glutamic oxalacetic transminase (GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.

Silk Fibroin Microsphere and Its Characterization

( Kwang Gill Lee ),( Joo Hong Yeo ),( Yong Woo Lee ),( Hae Yong Kweon ),( Soon Ok Woo ) 한국잠사학회 2003 International Journal of Industrial Entomology Vol.6 No.2

Synthesis and characterization of phosphorescent iridium complexes of 6-chloro-3-phenylpyridazine and 3-chloro-6-(3^′-methoxy-phenyl)-4-methyl-pyridazine

Lee, Sang Jin,Sung Lee, Jae,Hwang, Kwang-Jin,Kim, Young Kwan,Kim, Young Sik,Park, Noh Gill,Shin, Eun Ju,Lee, Seung Hee Elsevier 2005 CURRENT APPLIED PHYSICS Vol.5 No.1

<P><B>Abstract</B></P><P>The preparation and photophysics of organometallic Ir(III) complexes with 3-phenylpyridazine (H6Clppdz) and its derivative are reported. IrCl<SUB>3</SUB>·<I>n</I>H<SUB>2</SUB>O reacts with H6Clppdz, and 3-chloro-6-(3<SUP>′</SUP>-methoxyphenyl)-4-methyl-pyridazine (MCOMppdz) to give the corresponding chloride-bridged dimers, (6Clppdz)<SUB>2</SUB>Ir(<I>μ</I>-Cl)<SUB>2</SUB>Ir(6Clppdz)<SUB>2</SUB> and (MCOMppdz)<SUB>2</SUB>Ir(<I>μ</I>-Cl)<SUB>2</SUB>Ir(MCOMppdz)<SUB>2</SUB> in good yields. These chloride-bridged dimers are cleaved with 2-pyrazinecarboxylic acid (Hpyzc), 2-picolinic acid (Hpic), acetylacetone (Hacac), and 2,2,6,6-tetramethyl-3,5-heptanedione (Htmd) to give the corresponding monomer, (6Clppdz)<SUB>2</SUB>Ir(pyzc), (6Clppdz)<SUB>2</SUB>Ir(pic), (6Clppdz)<SUB>2</SUB>Ir(acac), (6Clppdz)<SUB>2</SUB>Ir(tmd), (MCOMppdz)<SUB>2</SUB>Ir(acac), and (MCOMppdz)<SUB>2</SUB>Ir(tmd), respectively. The complexes show bright orange to red luminescence at room temperature and the emission wavelenghts are affected by the ancillary ligands as well as cyclometalating ligands (593–664 nm).</P>

Effects of Silk Fibroin on Oxidative Stress and Membrane Fluidity in Brain of SD Rats

Lee,Jong Soo,Kim,Dae Ik,Kim,Jeung Min,Park,Soo Hyun,Choi,Jin Ho,Lee,Kwang Gill,Yeo,Joo Hong,Lee,Yong Woo 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.29 No.-

This study was designed to investigate the effects of silk fibroin powder(Mw 500) on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley (SD) male rats (160±10g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0g/㎏ BW/day for 6 weeks. Cholesterol level was significantly decreased about 8.0% in brain microsomes of SFP-5.0 group only compared with control group. Membrane fluidities were significantly increased (12.9% and 15.2%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 groups, but significant difference between in brain mitochondria of these two groups could be not obtained. Basal oxygen radicals (BOR) in brain mitochondria and microsomes were significantly inhibited (10.4% and 24.0%, 7.9% and 14.9%, respectively) by SFP-2.5 and SFP-5.0 groups compared with control group. Induced oxygen radicals (IOR) in brain mitochondria and microsomes were significantly inhibited (11.8% and 14.1%, respectively) by SFP-5.0 groups compared with control group compared with control group. Lipid peroxide (LPO) levels were dose-dependently decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (15.7% and 17.1%, 16.7% and 15.7%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that administration of SFP may play an effective role in a attenuating a oxidative stress and increasing a membrane fluidity in brain membranes.

Effects of Silk Fibroin on Oxygen radicals and Their Scavenger Enzymes in Brain of SD Rats

Lee,Jong Soo,Kim,Dae Ik,Kim,Jeung Min,Park,Soo Hyun,Choi,Jin Ho,Cho,Weon Ki,Lee,Kwang Gill,Yeo,Joo Hong,Lee,Yong Woo 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.29 No.-

This study was designed to investigate the effects of silk fibroin (Mw 500) powder (SFP) on oxygen radicals and their scavenger enzymes in brain membranes of rats. Sprague-Dawley (SD) male rats (160±10g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/㎏ BW/day for 6 weeks. Hydroxyl radical (·OH) levels resulted in a considerable decreases (6.6% and 9.7%, 2.8% and 11.9%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, but were significantly decreased in these membranes of SFP-5.0 group only. Superoxide radical (0₂￣) levels were a slightly decreased (2.0% and 9.1%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide (LPO) levels were significantly decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (16.7% and 15.7%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, but significant difference between in brain mitochondria of these two groups could not be obtained. Mn-SOD activities were remarkably increased (11.2% and 24.2%, respectively) in mitochondria of SFP-2.5 and SFP-5.0 groups. Cu,Zn-SOD activities were effectively increased (7.7% and 19.6%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups, but significant difference between control and SFP-2.5 groups could be not obtained. GSHPx activities were considerably increased (5.3% and 11.7%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that anti-aging effect of silk fibroin may play an effective learning and memory role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in brain membranes.

Effects of Silk Fibroin on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats

Lee,Jong Soo,Kim,Dae Ik,Kim,Jeung Min,Park,Soo Hyun,Choi,Jin Ho,Lee,Kwang Gill,Yeo,Joo Hong,Lee,Yong Woo 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.29 No.-

This study was designed to investigate the effects of silk fibroin (Mw 500) powder (SFP) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley (SD) male rats (160±1Og) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0g/㎏ BW/day for 6 weeks. Hydroxyl radical (·OH) levels resulted in a considerable decreases (5.8% and 8.4%, 3.7% and 11.1%, respectively) in liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, and O₂￣ radical level was remakably decreased about 15% and 20% in liver cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide (LPO) levels were significantly decreased (8.3% and 18.0%, 13.4% and 18.4%, respectively) in liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were remarkably decreased about 19.0% and 24.4% in liver microsomes of SFP-2.5 and SFP-5.0 groups, but significantly decreased about 11.6% in liver mitochondria of SFP-5.0 group compared with control group. Mn-SOD activities were remarkably increased (17.6% and 28.8%, respectively) in mitochondria of SFP-2.5 and SFP-5.0 groups, and Cu,Zn-SOD activities were also effectively increased (6.0% and 14.4%, respectively) in liver cytosol of SFP-2.5 and SFP-5.0 groups, but significant difference between GSHPx activity in liver cytosol of these two groups could be not obtained. These results suggest that anti-aging effect of silk fibroin may play an effective anti-aging role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in liver membranes.

The Improvement of Learning and Memory Ability of Normal Persons by BF-7

Lee, Sang-Hyung,Kim, Yong-Sik,Kang, Yong-Koo,Kwon, Oh-Sang,Shin, Yong-Kyoo,Song, Jln-Ho,Lee, Moo-Yeol,Lee, Kwang-Gill,Yeo, Joo-Hong,Lee, Won-Bok,Lee, Tae-Jin,Kim, Sung-Su The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.6

To investigate whether BF-7, extracted from Bombyx mori, improved learning and memory of ordinary people, Rey-Kim Memory Test was performed with placebo group (32 persons), 200 mg BF-7 treated group (33 persons) and 400 mg BF-7 treated group (34 persons). BF-7 enhanced significantly learning and memory function in a dose-dependent manner. To know how BF-T plays such a positive role, we measured the concentration of acetylcholine in the brain from memory impairment animal model. Treatment of BF-7 significantly increased the concentration of acetylcholine. So, it is supposed that the protection of cholinergic neuron and/or keeping proper concentration of acetylcholine might be one of the mechanisms by which BF-7 improve brain function. So, our results suggest that the BF-7 is effective material for improving learning and memory ability.

Silk Fibroin Gel의 제조조건에 따른 구조특성

이광길 ( Kwang Gill Lee ),이용우 ( Young Woo Lee ),여주홍 ( Joo Hong Yeo ),남진 ( Jin Nam ),권해용 ( Hae Young Kweon ),박영환 ( Young Hwan Park ) 한국잠사학회 1999 한국잠사곤충학회지 Vol.41 No.1

<논문(論文)> : 천잠 누에고치의 일반 성상

이광길 ( Kwang Gill Lee ),정다은 ( Da Eun Chung ),김기영 ( Kee Young Kim ),조유영 ( You Young Jo ),김현복 ( Hyun Bok Kim ),김성국 ( Sung Kuk Kim ),권해용 ( Haeyong Kweon ) 한국잠사학회 2015 한국잠사곤충학회지 Vol.53 No.1

국내에서 생산한 천잠 누에고치의 일반 특성을 고찰한 결과 국내산 천잠 누에고치는 연두색 고치를 지으며, 층상구조를 가지고 있었다. 천잠 누에고치는 외피는 연두색이었고 내피는 흰색을 나타내어 천잠 누에고치의 색상을 나타내는 색소 성분은 외피에 존재하였다. 천잠 누에고치는 고치층의 무게는 0.528 g, 견층두께는 0.424 mm로 측정되었다. 천잠 누에고치를 구성하는 주요 아미노산은 알라닌, 글리신, 세린, 아스파르트산, 티로산, 아르기닌 순으로 나타났으며, X-선 회절분석 결과 2θ = 16.8°, 20.4° 부근에서 강한 회절 피크와 2θ = 15.0°, 24.3°, 30.0° 부근에서 날카로운 회절 피크를 나타내었다. 천잠 누에고치는 폭이 20 μm 정도인 섬유가 적층된 구조를 가지고 있으며, 고치의 안쪽과 바깥쪽에 흰색 결정을 가지고 있었다. 천잠 누에고치의 최대 열분해 온도는 370℃ 부근이었다. 이러한 천잠 누에고치에 대한 연구 결과는 향후 천잠 누에 고치를 이용한 소재 개발의 기초 자료로 활용될 수 있을 것으로 기대한다. Antheraea yamamai silkworm cocoon is considered as a natural biocompatible materials, but can not be used as medical resources due to its difficult processability. In this study, we examined the general characteristics including cocoon shell weight and structural and thermal properties of Antheraea yamamai silkworm cocoon. The cocoon shell weight and thickness of wild silkworm cocoon was 0.528 g and 0.424 mm, respectively. The cocoon has yellow-green color in outside cocoon but white in inner layer cocoon. Amino acid analysis showed that the main amino acid of Antheraea yamamai cocoon is alanine, glycine, serine, aspartic acid, tyrosine and arginine. X-ray diffractometry showed that strong diffraction peaks at 2θ = 16.8°, 20.4°, corresponded to β-sheet structure and sharp diffraction peaks at 2θ = 15.0°, 24.3°, 30.0° due to the presence of calcium oxalate on cocoon surface. Antheraea yamamai cocoon was degraded at 370℃ These results might be used as basic information for development of non-textile materials using Anthereae yamamai silkworm cocoon.

세리신 단백질의 생리활성과 피부친화성 탐색

이광길 ( Lee Kwang Gill ),여주홍 ( Yeo Joo Hong ),이용우 ( Lee Yong Woo ),권해용 ( Kweon Hae Yong ),김종호 ( Kim Jong Ho ) 한국잠사학회 2001 한국잠사곤충학회지 Vol.43 No.2

실크 세리신 단백질을 추출정제하여 새로운 기능성 소재로 활용하기 위하여 세리신 추출법에 따른 세리신의 특성을 분석하고 약리효능과 피부친화성 소재로의 응용 가능성을 관능평가를 통하여 조사한 결과 다음과 같은 결론을 얻었다. 1. 실크 세리신 단백질을 효과적으로 추출하는 방법은 NaOH(0.02wt.%)로 처리하는 방법과 질소공기 하에서 식용효소인 Flavourzyme 3%로 처리하는 방법이 연감율 25wt.% 내외로 거의 100% 세리신을 회수하엿다. 2. 추출된 세리신의 분자량 분포는 10,000-30,000 범위였으며 용해도는 90 wt.% 이상이었다. 또한 Actinase 등 단백질 분해 효소로 처리하여 분자량 2,700-4,200의 저분자화 세리신을 얻을 수 있었으나 아미노산 조성은 고온고압 추출 세리신과 큰 차이가 없었다. 3. 세리신의 항산화, 혈당강하 및 알콜대사에 미치는 영향을 살펴본 결과 유의성있는 항산화 효과를 나타내지는 않았으나 세리신 투여시 sucrose와 maltose에 의하여 유발되는 혈당을 각각 62.6%, 41.5% 낮추는 효과를 나타내었다. 4. 피부친화성 소재로서 세리신의 활용가능성을 검토하기 위하여 중금속 함량을 측정한 결과 활성탄이나 wool filter를 이용함으로서 효과적으로 중금속을 제거할 수 있었으며, 세리신을 포함한 비누와 입욕제에 대한 관능평가 결과 세정력, 기포성, 세면후 피부보습성 등이 우수한 것으로 판정되어 실크 세리신 단백질은 피부친화성 미용 소재로 적용이 가능할 것으로 확인되었다. Silk sericin protein was extracted by treatment with enzyme or NaOH solution from raw silk and cutted-cocoon shell. The extracted sericin was characterized and examined the functional properties as well as subjective properties for its use as a soap and a body cleaner. The optimum extraction conditions on specimen are NaOH(0.02wt.%) or exzyme, Flavourzyme 3% under N2 gas. Molecular weights of sericin was decreased by treatment with enzyme, Actinase, from 10,000-30,000 to 2,700-4,200. Sericin showed important bioactive properties, for instance, lowering effect on blood glucose and alcohol. Subjective test of sericin soap and body cleaner showed superior in washability, foamability, and skin hydration, etc., to commercial soap and body cleaner. Therefore, it is though that silk sericin can be expected as the source of bioactive and skin-compatible materials.