방사선사진에서의 골질과 임상적으로 평가한 골질 분류의 상관관계

김현우,허경회,박관수,김정화,이원진,허민석,이삼선,최순철 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To investigate the validity of digital image processing on panoramic radiographs in estimating bone quality before endosseous dental implant installation by correlating bone quality in radiographic images with clinical bone quality classification. Materials and Methods : An experienced surgeon assessed and classified bone quality for implant sites with tactile sensation at the time of implant placement. Including fractal dimension eighteen morphologic features of trabecular pattern were examined in each anatomical sites on panoramic radiographs. Finally bone quality of 67 implant sites were evaluated in 42 patients. Results : Pearson correlation analysis showed that three morphologic parameters had weak linear negative correlation with clinical bone quality classification showing correlation coefficients of -0.276, -0.280, and -0.289, respectively (p<0.05). And other three morphologic parameters had obvious linear negative correlation with clinical bone quality classification showing correlation coefficients of -0.346, -0.488, and -0.343 respectively (p<0.05). Fractal dimension also had a linear correlation with clinical bone quality classification with correlation coefficients -0.506 significantly (p<0.05). Conclusion : This study suggests that fractal and morphometric analysis using digital panoramic radiographs can be used to evaluate bone quality for implant recipient sites. (Korean J Oral Maxillofac Radiol 2006; 36 : 25-32)

Leuconostoc mesenteroides B-512FMCM dextransucrase의 덱스트란 합성, 수용체 그리고 disproportionation 반응에서의 acarbose의 영향

김도만,류수진,박관하,ROBYT, JOHN F. 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-

Acarbose effectively inhibited the synthesis of dextran, and the inhibition pattern was a noncompetitive type with ?? value of 1.35mM. It also inhibited the disproport-ionation reaction of dextransucrase with isomaltotriose and decreased the efficiency of the maltose acceptor reaction. Increased concentration of dextransucrase or maltose in reaction digests, however, decreased the degree of inhibition by acarbose.

인라인 스케이트 이용자들의 손상 유형과 위험 요인

서혜경,문지영,성주욱,김말영,이준호,박정이,차성화,백나영,이재민,김수민,임현술,정해관,배근량,정철 東國大學校醫學硏究所 2004 東國醫學 Vol.11 No.2

지난 몇 년 사이 인라인 스케이트는 젊은층을 중심으로 인기있는 스포츠가 되었으며 이러한 대중화와 더불어 인라인 스케이트와 관련된 사고 빈도도 증가하는 추세이다. 따라서 본 연구는 인라인 스케이트 이용에 따른 사고율과 이용습관 형태, 손상 유형 및 위험 요인을 알아보고자 시행되었다. 2003년 9월 14일부터 9월 24일까지 경주시 ○○초등학교 6학년, ○○중학교 1학년, ○○여자고등학교 1학년, ○○고등학교 1학년, 동국대학교 의과대학 의학과 학생, 경주황성·포항 해맞이 공원의 성인을 포함한 총설문 대상자 1,305명 중 인라인 스케이트 이용자 392명을 대상으로 단면 연구를 수행하였다. 조사에 앞서 병원 방문과 사전 조사를 시행 하였으며 자기 기입식 설문지를 통해 정보를 수집하였다. 초, 중, 고 인라인 스케이트 전체 이용률은 28.1% 사고율은 30.1%로 나왔으며 주로 젊은층에서 사고가 많았으며 나이가 증가할수록 빈도가 증가하였다. 보호 장비 착용률은 44.4%로 조사되었다. 손상 부위로는 무릎과 상지 부위가 많은 것으로 조사되었으며 손상 유형은 찰과상과 타박상이 많았다. 스케이트를 타기 시작한지 1개월 이내에서 사고를 가장 많이 당한 것으로 나타났다. 전체 부상자의 20%가 후유증이 있었던 것으로 나타났다. 본 연구에서 인라인 스케이트 이용자들의 사고율이 비교적 높음을 알 수 있었다. 손상 부위의 무릎 및 상지 집중 현상으로 인해 무릎과 상지 부위의 보호대 착용이 필요할 것으로 생각되며 또한 사고를 줄이기 위해 초기 기술 습득 단계부터 전문가를 통한 체계적 교육과 안전 의식 고양이 필요할 것으로 보인다. In the past several years, Inline skating has become a popular sport among young people. With popularization of Inline skating, the number of injuries has increased proportionately. The purpose of this study is to evaluate the prevalence of injuries, the behaviors of skating, the nature and risk factors of injuries. We conducted a questionnaire an 1,305 subjects from Sep. 14 to 24, 2003. The Inline skaters were 392. These participation were mainly students from ○○elementary school, ○○middle school, ○○women's high school, ○○high school, Dongguk medical school, and adult skaters in several parks. The prevalence of injuries were 30.1%. Injuries were predominant from younger age and frequencies were increased according to increasing age. The most common location of injuries were both knee and the upper extremities. Most of injury type were skin abrasion and contusion. Injuries were highest within one month after First skating. 20% of injuries experienced a sequela. The results of this study show the severity of Inline skating-related injuries. We expect that the knee and the upper extrimities protectors should be used adequatly. Also to reduce injuries, when the skills are obtainded, the specilized learning programs and safety awareness are needed.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

Stimuli‐Responsive Reversible Fluorescence Switching in a Crystalline Donor–Acceptor Mixture Film: Mixed Stack Charge‐Transfer Emission versus Segregated Stack Monomer Emission

Park, Sang Kyu,Cho, Illhun,Gierschner, Johannes,Kim, Jin Hong,Kim, Jong H.,Kwon, Ji Eon,Kwon, Oh Kyu,Whang, Dong Ryeol,Park, Jung‐,Hwa,An, Byeong‐,Kwan,Park, Soo Young WILEY‐VCH Verlag 2016 Angewandte Chemie Vol.55 No.1

<P><B>Abstract</B></P><P>We report on a molecularly tailored 1:1 donor–acceptor (D‐A) charge‐transfer (CT) cocrystal that manifests strongly red‐shifted CT luminescence characteristics, as well as noteworthy reconfigurable self‐assembling behaviors. A loosely packed molecular organization is obtained as a consequence of the noncentrosymmetric chemical structure of molecule <B>A1</B>, which gives rise to considerable free volume and weak intermolecular interactions. The stacking features of the CT complex result in an external stimuli‐responsive molecular stacking reorganization between the mixed and demixed phases of the D‐A pair. Accordingly, high‐contrast fluorescence switching (red↔blue) is realized on the basis of the strong alternation of the electronic properties between the mixed and demixed phases. A combination of structural, spectroscopic, and computational studies reveal the underlying mechanism of this stimuli‐responsive behavior.</P>

Molecular cloning and identification of digestive proteases from aquatic insects; Laccotrephes japonensis, Scott,Ranatra unicolor, Muljarus japonicus.

Kwan-Ho Park,Seong-Ryul Kim,Eun-Young Yun,Hwa-Jin Suh,Si-Kab Nho,Jae-Sam Hwang 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05

Protease from various sources have been studied biotecnologically. For biotechnological applications, one highly preferred enzyme is protease. There have been no reports of cloned genes encoding digestive proteases in the Laccotrephes japonenis, Ranatra unicolor, Muljarus japonicus. These insects are considered to be a predator of aquatic insects. RT-PCR was used to amplify cDNA fragments for digestive proteases from total RNA the hole body of the insects. The flanking sequences of the 5'- and 3'- end of the these genes were characterized by RACE-PCR. Sequence analysis showed that these genes contained complete ORF. The deduced amino acid sequences of these protease showed 62% identity to the serine protease of Creontiades dilutus, 58% to Lygus loneolaris trypsin-like serine proteinase , 54% to Triatonatoma infestans salivary trypsin. In the further study, to generate digestive protease, the DNA fragment coding for serine protease, trypsin-like serine protease were cloning into suttle vector pBACⅠ, and infected to Spodoptera frugiperda (sf9) insect cell. After that, we expect to carry out the proteolytic activity of these recombinant proteases. This is intended as a basis for future studies on the digestive protease in the insects.

Kinetics for Steam and CO2 Reforming of Methane Over Ni/La/Al2O3 Catalyst.

Park, Myung Hee,Choi, Bong Kwan,Park, Yoon Hwa,Moon, Dong Ju,Park, Nam Cook,Kim, Young Chul American Scientific Publishers 2015 Journal of nanoscience and nanotechnology Vol.15 No.7

<P>Kinetic studies of mixed (steam and dry) reforming of methane on Ni/La/Al2O3 and Ni/La-Co (1, 3 wt%)/Al2O3 catalysts were performed in an atmospheric fixed-bed reactor. Kinetic parameters for the mixed reforming over these catalysts were obtained under reaction conditions free from heat and mass transfer limitations. Variables for the mixed reforming were the reaction temperature and partial pressure of reactants. The fitting of the experimental data for the rate of methane conversion, rCH4, using the power law rate equation rCH4 = k(PrCH4)관(PCO2)관(PH2O)관 showed that the reaction orders 관, 관, and 관 are steady and obtained values equal to 관 = 1, 관 = 0, and 관 = 0. In other words, among CH4, CO2, H2O, and H2, only CH4 reaction orders were not zero and they were affected by the promoters. The apparent activation energy on catalysts Ni/La/Al2O3, Ni/La-Co (1)/Al2O3 and Ni/La-Co (3)/Al2O3 is 85.2, 93.8, and 99.4 kJ/mol, respectively. The addition of Co to Ni/La/Al2O3 was increased the apparent activation energy of the mixed reforming reaction. And the Ni/La-Co (3 wt%)/Al2O3 catalyst showed the highest reforming activity and apparent activation energy. The Co promoters can increase the apparent activation energy of mixed reforming of methane.</P>

Molecular cloning, gene structure, expression and enzyme activity of a serine-like protease from Laccotrephes japonensis

Kwan-Ho Park,Seong-Ryul Kim,Eun-Young Yun,Hwa-Jin Suh,Si-Kab Nho,Jae-Sam Hwang 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

The morecular cloning, gene structure, expression and enzyme activity of a serine-like proteas frome Laccotrephes Japonensis were examined. In this study, RT-PCR was used to amplify cDNA fragments for serine-like proteases from total RNA the hole body of Laccotrephes japonensis. The flanking sequences of the 5'- and 3'- end of the this gene were characterized by RACE-PCR. Sequence analysis showed that this gene contained an 963bp ORF encoding 321 amino acids. The deduced amino acid sequence of this protease showed 62% identity to the serine protease of Creontiades dilutus, 58% to Lygus loneolaris trypsin precuror LlsgP4, 54% to Triatonatoma infestans salivary trypsin. To generate Laccotrephes japonensis serine-like protease, the DNA fragment coding for serine protease is cloning into suttle vector pBACⅠ, named pBAC1-JG and infected to Spodoptera frugiperda (sf9) insect cell. The cDNA encoding JG was expressed as a 32-kDa polypeptide in baculovirus infected insect cells and the recombinant JG showed activity in the protease enzyme assay using gelatin as a substrate.

Characterization of an exo-acting intracellular α-amylase from the hyperthermophilic bacterium Thermotoga neapolitana

Park, Kyung-Min,Jun, So-Young,Choi, Kyoung-Hwa,Park, Kwan-Hwa,Park, Cheon-Seok,Cha, Jaeho Springer-Verlag 2010 Applied microbiology and biotechnology Vol.86 No.2

Effects and Optimization of Selection Pressure in Enzyme Production Using Recombinant E .

Park, Kwan Hwa,YOO, YOUNG JE,Lee, Jung Heon 한국화학공학회 1991 NICE Vol.9 No.3

Selection pressure is widely used in stabilizing the plasmid of the recombinant microorganism. The effects of selection pressure on enzyme production were investigated using recombinant E. coli harboring plasmid pTA322. Optimum ampicillin concentration. which maximizes the enzyme production was 150 pg /ml in flask culture and 50 pg/ml in the culture using 2-1 fermentor. Optimum concentration or selection pressure in a stirred fermentor was different from that in a flask due to different agitation effects. It was found from mathematical modelling that as the concentration of ampicitlin increased, the plasmid-harboring cells with low plasmid copy number were damaged severely, while the specific production rate increased due to the high plasmid copy number in flue microorganism.