Poster Presentations : P18 ; Stimulation by Parabens Up-Regulate TSLP Expression in Human Normal Keratinocytes

( Kkot Byeol Kim ),( Seung Hi Lee ),( Mi Jin Jang ),( Sun A Lee ),( Hyun Jung Kim ) 한국피부장벽학회 2013 한국피부장벽학회지 Vol.15 No.2

Some ingredients of dermatological formulations result in skin irritation and allergy. In particular, preservatives have been reported extensively as a cause of allergic contact dermatitis. The study focused on parabens which have been used extensively as antimicrobial preservatives in foods, drugs and cosmetics. Thymic stromal lymphopoietin (TSLP) is an IL-7 related cytokine, produced by epithelial cells, that has been linked to atopic dermatitis and asthma. TSLP expression is up-regulated in the lesions of allergic patients. Here we demonstrate a change of TSLP expression when stimulated by parabens in human normal keratinocytes. Methyl paraben(MP), propyl paraben(PP) and butyl paraben(BP) of various parabens are used widely. The study investigated the effects exposure to MP, PP and BP on keratinocytes in vitro. Normal human keratinocytes was cultured in the medium containing MP, PP and BP. The following changes were analysed: proliferating ability, TSLP mRNA expressions. MP, PP and BP decreased the proliferating ability of keratinocytes especially PP and BP affect at low concentration rather than MP. And PP and BP increase the expressions of TSLP mRNA in keratinocytes but MP doesn`t. Interestingly effect of PP and BP that increased the expressions of TSLP mRNA confirmed in allowed concentration for use in cosmetics products by Ministry of Food and Drug Safety. These results suggest that parabens which have been used in cosmetics might influence the atopic dermatitis through decreased the proliferating ability and increased the expressions of TSLP mRNA of keratinocytes.

Neuroprotective effects of urolithin A on H₂O₂-induced oxidative stress-mediated apoptosis in SK-N-MC cells

Kkot Byeol Kim,Seonah Lee,Jung Hee Kim 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.1

BACKGROUND/OBJECTIVES: Oxidative stress causes cell damage and death, which contribute to the pathogenesis of neurodegenerative diseases. Urolithin A (UA), a gut microbial-derived metabolite of ellagitannins and ellagic acid, has high bioavailability and various health benefits such as antioxidant and anti-inflammatory effects. However, it is unknown whether it has protective effects against oxidative stress-induced cell death. We investigated whether UA ameliorates H₂O₂-induced neuronal cell death. MATERIALS/METHODS: We induced oxidative damage with 300 μM H₂O₂ after UA pretreatment at concentrations of 1.25, 2.5, and 5 μM in SK-N-MC cells. Cytotoxicity and cell viability were determined using the CCK-8 assay. The formation of reactive oxygen species (ROS) was measured using a 2,7-dichlorofluorescein diacetate assay. Hoechst 33342 staining was used to characterize morphological changes in apoptotic cells. The expressions of apoptosis proteins were measured using Western blotting. RESULTS: UA significantly increased cell viability and decreased intracellular ROS production in a dose-dependent manner in SK-N-MC cells. It also decreased the Bax/Bcl-2 ratio and the expressions of cytochrome c, cleaved caspase-9, cleaved caspase-3, and cleaved PARP. In addition, it suppressed the phosphorylation of the p38 mitogen-activated protein kinase (MAPK) pathway. CONCLUSIONS: UA attenuates oxidative stress-induced apoptosis via inhibiting the mitochondrial-related apoptosis pathway and modulating the p38 MAPK pathway, suggesting that it may be an effective neuroprotective agent.

산화적 스트레스에 대한 여주 (Momordica charantia) 추출물의 항산화 효과 및 세포사멸 억제 기전을 통한 신경세포보호효과

김꽃별(Kkot Byeol Kim),이선아(Seonah Lee),허재혁(Jae Hyeok Heo),김정희(Jung hee Kim) 한국영양학회 2017 Journal of Nutrition and Health Vol.50 No.5

건 여주로부터 얻은 70%에탄올 추출물의 항산화 효과를 측정하고, H₂O₂에 의해 유도된 산화적 스트레스에 대한 신경세포 보호효과를 알아보기 위해 human neuroblastoma cell인 SK-N-MC세포를 이용하여 실험을 수행하였다. 여주 추출물의 총 폴리페놀과 플라보노이드 함량은 각각 28.51 mg gallic acid/extract g과 3.95 mg catechin/extract g 이었고, 추출물의 DPPH 라디칼 소거능 (IC50)은 506.95 μg/ml 이었다. 여주추출물을 신경세포에 전 처리한 후 H₂O₂을 처리하여 산화적 스트레스를 유도했을 때, 여주 추출물에 의해 세포생존율은 증가되었고 세포내 ROS는 감소되는 것을 확인하였다. 그리고 세포내 항산화 방어시스템인 항산화효소 (SOD-1,2와 GPx-1)의 mRNA 발현이 여주추출물 처리에 의해 control 수준으로 회복되거나 control 보다 증가되는 결과를 보였으며, ROS 의존적 세포사멸과 연관 있는 것으로 알려진 MAPK pathway 중 p38과 JNK의 인산화를 여주추출물이 억제하였다. 또한 cleaved caspase-3와 cleaved PARP의 발현도 여주추출물의 처리에 의해 감소되었다. 본 연구 결과에서 70% 에탄올 여주추출물은 항산화효능이 우수하여 ROS를 직접적으로 제거할 뿐 아니라 세포내 ROS 축적을 억제시키는 효과를 보여주었다. 그리고 신경세포 내 항산화효소들의 발현 증가 기전과 p38, JNK의 인산화 억제 및 cleaved caspase-3, cleaved PARP의 발현 억제를 통한 세포사멸 억제 기전을 통해 산화적 스트레스로부터 신경세포를 보호하는 효과가 있음을 제시하고 있다. 따라서 여주추출물은 산화적 스트레스에 의한 알츠하이머병이나 파킨슨병 등과 같은 신경변성질환 (neurodegenerative disease)에 대한 예방 및 치료제의 소재로써 이용가치가 충분한 것으로 사료된다. Purpose: Many studies have suggested that neuronal cells protect against oxidative stress-induced apoptotic cell death by polyphenolic compounds. We investigated the neuroprotective effects and the mechanism of action of Momordica charantia ethanol extract (MCE) against H₂O₂-induced cell death of human neuroblastoma SK-N-MC cells. Methods: The antioxidant activity of MCE was measured by the quantity of total phenolic acid compounds (TPC), quantity of total flavonoid compounds (TFC), and 2,2-Diphenyl-1-pycrylhydrazyl (DPPH) radical scavenging activity. Cytotoxicity and cell viability were determined by CCK-8 assay. The formation of reactive oxygen species (ROS) was measured using 2,7- dichlorofluorescein diacetate (DCF-DA) assay. Antioxidant enzyme (SOD-1,2 and GPx-1) expression was determined by real-time PCR. Mitogen-activated protein kinases (MAPK) pathway and apoptosis signal expression was measured by Western blotting. Results: The TPC and TFC quantities of MCE were 28.51 mg gallic acid equivalents/extract g and 3.95 mg catechin equivalents/extract g, respectively. The IC50 value for DPPH radical scavenging activity was 506.95 μg/ml for MCE. Pre-treatment with MCE showed protective effects against H₂O₂-induced cell death and inhibited ROS generation by oxidative stress. SOD-1,2 and GPx-1 mRNA expression was recovered by pre-treatment with MCE compared with the presence of H₂O₂. Pre-treatment with MCE inhibited phosphorylation of p38 and the JNK pathway and down-regulated cleaved caspase-3 and cleaved PARP by H₂O₂. Conclusion: The neuroprotective effects of MCE in terms of recovery of antioxidant enzyme gene expression, down-regulation of MAPK pathways, and inhibition apoptosis is associated with reduced oxidative stress in SK-N-MC cells.

한국 성인의 음주, 흡연, 운동 및 영양행태에 대한 군집별 특성 및 관련요인

김꽃별(Kkot-byeol Kim),은상준(Sang Jun Eun) 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.5

본 연구의 목적은 한국 성인의 건강행태군집 유형을 확인하고 이와 관련된 요인들을 파악하는 것이다. 이를 위해 2014년도 국민건강영양조사 자료를 이차분석 하였다. 음주, 흡연, 운동 및 영양 변수를 이용하여 19세 이상 성인을 대상으로 군집분석하였고, 이 유형들과 인구사회학적 특성 및 건강상태의 연관성을 확인하기 위해 SPSS WIN 22 복합표본설계 모듈을 이용하여 카이제곱 검정과 다항로지스틱 분석을 실시하였다. 한국 성인의 건강행태군집 유형은 흡연군, 식습관군, 건강 관심군, 수동적 태도군 그리고 음주군의 총 6개 유형으로 분류되었고 수동적 태도군의 빈도가 26.0%로 가장 높았다. 건강행태 군집의 타당성을 검증하기 위해 분류된 군집과 건강행태변수를 이용해 판별분석한 결과 타당한 것으로 나타났다. 건강행태 군집은 성, 연령, 교육 수준, 직업, 소득 수준, 결혼 상태 및 지역 규모등 인구 사회학적 특성별로 분포가 달랐으며, 고혈압 및 당뇨와 같은 일부 만성질환의 유무별로도 다른 것으로 나타났다. 인구사회학적 특성, 지역, 고혈압 및 당뇨는 수동적 태도군 보다는 나머지 건강행태군집에 속하는 것과 유의한 연관을 보이는 변수였다. 본 연구 결과는 건강행태를 개별적으로 접근하기 보다는 군집으로 접근하는 것의 유용성에 대한 근거 자료로 활용할 수 있을 것이다. The purpose of this study was to identify the type of health behaviors in Korean adults and to identify related factors. The data used in the analysis was the Korea Health and Nutrition Examination Survey 2014., which was representative of the Korean population. Cluster analysis was used to find the pattern of clustering of smoking, drinking, exercising and nutrition. Differences in the pattern of clustering was examined, first by bivariate chi-square test, and then by multinomial logit regression. Lastly, the association between the clusters of health behaviors and other behavioral risk factors was tested by chi-square test and logistic regression. The distribution of the clusters varied not only across socioeconomic characteristics and local size, but also between individuals with certain chronic diseases and those without. The results of this study can be used as a basis for the usefulness of approaching the cluster rather than individually approaching the health behavior.

Poster Presentations : P17 ; The Effect of Novel Cannabinoid Receptor Agonists on the Oxazolone Induced Atopic Dermatitis Mouse Model

( Hyun Jung Kim ),( Se Kyoo Jeong ),( Seung Phil Hong ),( Seung Hee Lee ),( Kkot Byeol Kim ),( Mi Jin Jang ),( Seon Ah Lee ),( Sung Gu Ahn ) 한국피부장벽학회 2013 한국피부장벽학회지 Vol.15 No.2

The newly discovered endocannabinoid system(ECS) has been implicated in multiple regulatory functions both in health and disease. Two cannabinoid receptor, CB1 and CB2, is important receptor to be partly ECS. Recent studies have suggested the existence of a functional ECS in the skin and implicated it in various biological processes. The expression of CB1 according to differentiation pattern of normal human keratinocyte was examined in calcium induced2D model and 3D culture model.Modulation of the fine tuned tone of the cutaneous endocanabinoid system could have therapeutic values in the management of a large variety of human skin disease. Endocannabinoids limit excessive mast cell maturation and activation in human skin CB1 receptors are functionally expressed by KCs in vivo and help to limit the secretion of proinflammatory chemokines that regulate T cell.dependent inflammation in the effector phase of CHS. Based on the anti-inflammatory effect and barrier prone effect of CB1 agonist , we tried to check out the possibility of new CB1 agonists for AD treatment using Oxazolone-AD Model. Topical CB1r agonist showed significant anti-inflammatory effects in Oxa induced murine AD model. Recovery of epidermal permeability barrier function was also accelerated by CB1R-agonist. Hyper-proliferation and abnormal differentiation were attenuated by co-application of CB1R-agonist. Epidermal expression of IL-8 was down-regulated by CB1R-agonist. CB1R is involved in AD pathogenesis and CB1R-agonist might be a therapeutic regiment for AD.

Poster Presentations : P8 ; Evaluation of Anti-inflammatory Activity of N-Oleoylethanolamine (NOE) as a Topical Agent

( Seung Hee Lee ),( Kkot Byeol Kim ),( Mi Jin Jang ),( Sun A Lee ),( Sung Ku Ahn ),( Se Kyoo Jeong ),( Seung Phil Hong ),( Hyun Jung Kim ) 한국피부장벽학회 2014 한국피부장벽학회지 Vol.16 No.2

Biological activities of NOE (N-oleoylethanolamine) encompass inhibiting the ceramidase activity and stimulating serine palmitoyl transferase (SPT), which is a key enzyme for in situ ceramide synthesis. As a result, treatment of NOE induces accumulation of ceramide in cells. Previously, it was reported that treatment of NOE on cultured normal human epidermal keratinocyte (NHEK) resulted in the increased expression of the differentiation marker proteins, including involucrin and transglutaminase-1, which suggest a potential application of NOE for normalizing the hyper proliferation in inflammatory skin diseases. We have also reported that the topical application of cannabinoid receptor 1 (CB1R) agonist alleviated the inflammatory symptoms in atopic dermatitis animal model. Based on the structural similarity between NOE and previously reported CB1 agonist, it is hypothesized that topical application of NOE can be a therapeutic candidate for atopic dermatitis, at least in part, through the activation of CB1R. As results, topical application of NOE significantly accelerated the epidermal permeability barrier recovery after acute disruption. Anti-inflammatory activity of NOE was also observed in both TPA-induced acute dermatitis model and oxazolone-induced atopic dermatitis animal model, which was also confirmed by histological assessment. It also downregulated the expression of TSLP. These results suggest that, NOE can be used as an anti-inflammatory agents for skin diseases including atopic dermatitis.

Surgical treatment of mandibular first premolar with radicular groove: periradicular microsurgery

최유리나,Sue A Kim,Kkot Byeol Bae,Hoon-Sang Chang 조선대학교 치의학연구원 2024 Oral Biology Research (Oral Biol Res) Vol.48 No.1

This study aimed to report the results of a successful periradicular microsurgery on a mandibular first premolar with a radicular groove. A 38-year-old female reported a sinus tract on her buccal gingiva which was radiographically traced to the middle third of the mesial side of the root of her mandibular right first premolar (#44). A radicular groove and an accessory canal in the middle of the radicular groove were found using cone-beam computed tomography. After endodontic treatment, the sinus tract did not disappear; thus, periradicular microsurgery was performed. The radicular groove and the accessory canal were prepared and filled with fastsetting MTA without resectioning the root apex. After 3 weeks, the sinus tract disappeared, and complete healing was revealed on the periapical radiograph at a 5-month follow-up. Therefore, periradicular microsurgery is recommended in mandibular first premolars with radicular grooves on the accessible mesial side of the root.