烏骨鷄의 遺傳形質에 關한 硏究

李基萬,鄭吉生,韓相基 건국대학교 1981 學術誌 Vol.25 No.1

This study was conducted to research on hereditary characters of Korean native Ogolgyes, which mean black bone chickens, with 1080 adults and young chicks, body weight, body colors, colors of feathers, farms of body parts, blood types and reproducibility were investigated. The results obtained were summarized as follows; 1.Native Ogolgyes, as contour featherings, were a different kind of breed from western silkies, that is to say, silky feathering chickens. 2.Native ogologyes were not uniform in colors of feather, body colors, and forms of body parts and various characters were existed confusedly. 3.As for the domestic distribution of Ogolgyes, total 6.513 chickens were distributed in seven provinces, and of which the number of native Ogologyes was 3,728, western Ogolgyes 1,136, and these hybrids 1,649. 4.Native Ogolgyes body sizes were large and weighed 2.62 ± 0.24 kg in cocks, and 2.25 ± 0.30kg in hens. 5.Native Ogolgyes, haying eye color with black, expressed Ogolgyes' characteristics well, but parts of them (1.4%) had brown-black eye color. 6.Skin color was grayish blue, Comb, wattle, ear lobe, and face were nearly the same color, grayish black or mulberry approaching black. Shank and toe color was black or light black. Beak and toe-nail color was black, but these points were light horny yellow. But these varieties of colors were numerous. 7.The feather colors of Native Ogologyes were observed to be most diverse ; black color was dominating (95.2%), white was next (3.0%), and black confused with white were reserved to existed(1.9%). Young chick feathers were yellowish gray color or yellowish white color. 8.The percentage of crest was 24.6%, and crests were small sizes, crest of birds with beard was 14.6%, crest with only wattle beards was 3.2%, crest with muffs was 1.6% and nonbearded chickens were 80.6%. 9.In combs, the distribution of single comb was 89.9%, mulberry comb was 10.1%. Cock's single comb was erected and hen's was that the erect was 70% and the lopped was 30%. Shape and size of these combs were not uniform largely. Wattles were various type in size. 10.All the Ogologyes had four toes. 11.Color of tongue was gray. The peritoneum and pleura of body sides and out side membrane of gizzard were grayish black color. Muscle was a little gray, and periosteum was grayish blach, but when boiled, the color was changed to deep black. 12.The present study revealed 52 of Alb BB type and 1 of Alb BC type. The Alb AA homozygote phenotype has not been recognized in Korean Ogolgyes. The following gene frequencies were calculated; AlbA = 0.000 AlbB = 0.991 AlbC = 0.001. The distribution of Tf and Amy Phenotypes corresponds to the following gene frequencies: TfA = 0.000 TfB = 0.964 TfC = 0.036, AmyA = 0.937 AmyB = 0.045 AmyC = 0.018 Alleles TfB and AmyA had the highest frequencies in Ogolgyes, whereas TfA, AmyB and AmyC occur very rarely and the TfA was not recognized. 13.As for the reproducibility, the first egg laying age in month was six in average. The broodiness was steady. The numbers of laying eggs vearly were about 100. Fertility was 80.6 percent. Hatchability was 83.4 percent. Rearing rates were 30-50 percent. As for the eggs layed by hens, eggs weighed 49.18 ± 4.09 gram, 5.42 ± 0.26 cm in length, 3.90 ± 0.24 cm in width. Colors of egg shells were light brown largely, but showed great difference between thick and light respectively. According to these results, the Korean Native Ogolgyes were various in character and wanted in breeds' uniformity. Ogolgyes could be fixed four varieties of black feather's chickens, white feathers' by the color of feathers, of non-bearded and bearded chickens by the existence of beard. Of which, it is necessary to fix bearded black variety rapidly for commercial value.

大家畜의 受精卵 移植에 關한 硏究

李基萬,鄭吉生,高光斗 건국대학교 1982 學術誌 Vol.26 No.2

This study was carried out in order to induce multiple birth following egg transfer in Korean native cattle, and composed of three experiments ; induction of multiple birth following GTH single administration, Ovarian response to the administration of GTH in combination with Prostaglandin F2α and non-surgical egg transfer following superovulation and non-surgical recovery of fertilized eggs. The results obtained in three experiments were summarized as followings: Experiment I. Induction of multiple birth following GTH single administration. (1) Average duration from PMS administration to the onset of estrus was 3.8 to 4.7 days and no difference due to dosage and administration site of PMS were observed. (2) Duration of induced estrus was ranged from 3.1 to 4.6 days and that of induced estrous cycle was shortened by the length of induced estrus. (3) The most slowed follicles (5.3- 5.5) were observed in the cattle administrated with 2,000 IU of PMS and 1,000 IU of PMS was considered to be the optimum dosage for the limited ovulation. (4) Ovulation rate was declined when the PMS dosage was increased more than, 1000 IU (5) Highest rate of twin birth (35.7%) was obtained from the cattle treated with 1,000 IU of PMS and the next was the catties treated with 500 IU and 2,000 IU of PMS, respectively. Experiment II. Ovarian response to the administration of GTH in combination with Prostaglandin F2α. (1) Average duration from PMS administration to the onset of estrus was 5 days and the duration was shortened with the increasing dosage of PMS. (2) Duration of induced estrus was ranged from 2.3 to 4.5 days and the duration of estrus was shortened with the decreasing dosage of PMS. (3) Number of follicle obtained from the cattle treated with 4,000, 3,000, 2,000, 1,000 and 500 IU of PMS were clarified to be 11.4, 11.5,7,2.3 and 2.5, respectively, after 4.8-5.2 days of PMS administration. (4) Observing the results of slaughter, ovulation rates of the cattle treated with 4,000, 3,000, 2,000, 1,000, and 500 Iu of FMS were 16.3, 35.6, 60.0, 65 and 75%, respectively. (5) The numbers of degenerated, or bled follicle were increased with the increase of PMS dosage. Experiment III. Non-surgical egg transfer following superovulation and non-surgical egg transfer. (1) Average six eggs per donor were ovulated and 65% of them were recovered by non-surgical technique. (2) Eighty two percent of eggs recovered were found to be normal morphologically and were at 4-32 cell stage, respectively. (3) Seventy five percent of cattle treated with prostaglandin F2α were synchronized in their estrus within 4 days of administration. (4) No twin fetation was obtained from the five recipients received fertilized eggs.

pHOxsFV벡터와 배아주간세포를 이용한 형질전환생쥐 생산 기초연구

이훈택,이봄이,정길생,김진회 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

pHook™-1 hapten 4-ethoxy-methylene-2-phenyl-2-oxazolin-5one(phOX)의 단일 항체 sFV를 암호화 하고 있으며, murine의 Ig k-chain V-J2-C 영역유래의 signal peptide에 의하여 항체를 세포 표면에 배열시키도록 고안되어 있다. 또한, 항체를 세포막 바깥쪽에 부착되어 있도록 하기 위해 PDGFR 유래의 transembrane domain의 C 말단에 결합되어 있다. 이렇게 고안된 vector을 발현하는 세포는 세포막에 sFV을 발현함으로, phOX로 코팅된 자석베드를 이용하여 배양체로부터 목적의 유전자를 발현하는 세포만을 분리할 수 있을 것이다. 따라서, 본 연구는 pHook™-1 유전자를 co-transfection함으로써 목적의 유전자를 가진 배아주간세포를 단시간 내에 효율적으로 선발하기 위하여 실시하였다. 또한, 배아주간세포에서 목적의 DNA 발현 또는 존재를 검증하기 위해 DNA 발현 또는 존재를 검증하기 위해 PCR 방법과 조직화학적 방법을 사용하였다. 형질전환유전자 발현을 transfection(유전자 전이) 후 4∼14일 사이에 모든 배아주간세포에서 확인되었다. Magnetic bead를 이용하여 선발된 세포에서 co-transfected DNA는 배아주간세포에서 효율절으로 삽입되었으며, 선발된 세포의 약 90%는 co-transfected 유전자를 발현하였다. 이 결과는 세포생리학에서 특이 유전자의 급성변이와 만성변이를 연구하거나, 또는 형질전환동물을 생산하기 위해 pHook™-1 목적유전자와 함께 전이함으로서 효율적으로 목적의 유전자를 가진 세포를 선발 가능함으로써 보다 간편하게 형질전환도 동물의 생산에 이용 가능하다는 사실을 확인하였다. pHook™-1 encodes a single-chain antibody(sFv) directed toward the hapten 4-ethoxy-methylene-2-phenyl-2-oxazolin-5-one(phOx): the signal peptide from the murine Igk-chain V-J2-C region is fused in front of coding region of the sFv to direct the antibody to the plasma membrane. The antibody is fused at the C-termius to the transmembrane domain from the platelet derived growth factor receptor(PDGFR), allowing the antibody to be anchored and displayed on the extracellular side of theemmbrane. Transfected cells expression sFv can be isolated from whole cultures by using magnetic coated with phOx and a strong magnetic strand. Thus, the present study was designed to apply the embryonic stem cells by using pHook™-1 . Cell-transduction efficiency was measured by morphometric analysis. Polymerase chain reaction and histochemistry were used to detect the presence and/or expression of objective DNA in embryonic stem cells. Transgene expression was detected in all cases between 4 and 14 day after transfection. In selected cells using magnetic bead, co-transfected DNA was also incorporated efficiently in embryonic stem cells and approximately 90% of the selected cells expressed co-transfected gene. This result suggested that this selection system can be used as a feasible tool, when pHook™-1 is cotransfected with objective gene, to isolate and study for acute and chronic changes of a specific gene in cellular physiology.

韓牛 改良을 위한 屠體重 推定에 관한 硏究

李基萬,李黃田,鄭吉生 건국대학교 1977 學術誌 Vol.21 No.2

A total of 1627 Korean cattle were studied to measure live weight, withers height, body length, chest girth, belly girth, carcass weight and to calculate correlation coefficients among traits studied as well as to make linear regression equation. Among traits studied, the highest correlation coefficient was the correlation of chest girth with carcass weight. At interval of 10cm of chest girth, every regression equation was calculated, and according to this equation estimated carcass weights were tabled. 1.All average values of traits studied over 3 year cattle, the male values were higher than the female one. The larger variation of belly girth in female will be due to the cause of pregnant cows. 2.The correlation of chest girth with carcass weight was the highest of all traits studied, except for the trait of live weight. The correlation coefficients of chest girth with carcass weights were r = 0.693, r = 0.806 in both sexes, respectively. 3.The linear regression equations of live weight, withers height, body length, chest girth, belly girth on carcass weight were calculated. 4.Instead of complicated formulae, the simple regression equations of chest girth on carcass weight were calculated at intervals of 10cm chest girth and estimated carcass weight was tabled.

사슴의 내부기생충 감염에 관한 조사연구

윤화중,정길생,Yoon Hwa-Joong,Chung Kil-Saeng 대한수의사회 1983 대한수의사회지 Vol.19 No.7

The authors made a survey on the species and distribution of internal parasites in domesticated deer. Total 75 specimen which collected from Seoul City, Gyeonggi Do, Kangwon Do and Chung-Nam Province were carried out for the actual condition of parasitic

Mitochondrial DNA Heteroplasmy in Cloned Bovine Embryos following Somatic Cell Nuclear Transfer

Do Jeoneg Tae,Lee Bo Yon,Kim Seung Bo,Lee Hoon Taek,Chung Kil Saeng 한국동물번식학회 2002 Reproductive & Developmental Biology(Supplement) Vol.26 No.1s

The Mechanism of Lipid Efflux from Boar Spermatozoa by the Action of Lipid Decreasing Factor (Ⅰ)

Chung, Kil-Saeng 건국대학교 1974 學術誌 Vol.18 No.1

牛精漿中에 含有되어 있는 脂質減少要因의 作用에 의하여 豚精子中의 脂質含量은 顯著하게 減少한다. 이러한 現象은 同要因의 作用에 의하여 豚精子中의 脂質이 分解되거나 精子外로 漏出되기 때문일 것으로 推理하여 이 점을 究明하기 위하여 本硏究에서는 脂質減少要因의 生理的 特性을 調査하고 同要因의 作用에 의하여 豚精子中에 含有되어 있는 脂質의 漏出이 分解에 의한 것인지 또는 다른 原因에 의한 것인지를 檢討하였다. 本硏究에 의하여 얻어진 結果는 다음과 같다. 1) 牛精漿蛋白의 脂質減少活性은 蛋白量의 增加와 더불어 增加했다. 그러나, 豚精漿蛋白의 경우에는 그 量이 增加해도 脂質減少活性은 認定되지 않았다. 2) 脂質減少要因의 作用에 의한 精子中의 脂質含量의 減少는 그 대부분이 反應 10分 以內에 이루어졌다. 3) 70℃에 있어서의 30分間의 加熱處理에 의하여 脂質減少要因의 活性은 完全히 喪失되었다. 4) 脂質減少要因의 至適 pH는 11.5로 判明되었다. 5) 豚精子中에 含有되어 있는 脂質을 基質로 하여, Lipoprotein lipaseㆍLipaseㆍphospholipase A 및 Esterase 등과 같은 脂質分解酵素의 活性의 有無를 調査했으나 그러한 酵素活性은 認定되지 않았다. 6) 脂質減少要因에 脂質分解酵素의 活性을 抑制하는 阻害劑를 作用시킨 다음, 이것을 豚精子에 作用시켰으나, 脂質減少活性은 전혀 阻害되지 않았다. 7) 脂質減少要因의 作用에 의하여 淸子中의 Ester-型 總脂肪酸의 含量은 顯著하게 減少하고, 減少한 만큼 精子浮游液中에는 增加했다. 8) 以上의 結果로 脂質減少要因의 作用에 의하여 豚精子中의 脂質合量이 減少하는 것은 同要因의 作用에 의하여 精子中의 脂質이 分解되기 때문이 아니고, 精子中의 脂質이 Ester 結合을 保存한 채 精子外로 漏出되기 때문이라는 사실이 밝혀졌다. 동시에 脂質減少要因은 脂質을 分解하는 酵素가 아니라는 점도 밝혀졌다.

선진 유럽유기농업의 환경보전 기능과 안전농산물 생산 - 한국유기농업의 발전을 위한 농업정책적 제안 -

정길생 ( Chung Kil-saeng ),손상목 ( Sohn Sang-mok ),이윤건 ( Lee Yoon-gun ) 한국유기농업학회 1996 韓國有機農業學會誌 Vol.5 No.1

In Korea there is still no basic standard for organic agriculture and organic farmers in Korea do not follow the minimum requirements of IFOAM basic standard. Most of them just practice the organic agriculture applying organic fertilizer, commercial seed without legume, rotation and green manure. But they believe this system is a absolutely environmental friendly agricultural system and it produce a safe agricultural product since they are not aware of the basic standard of organic agriculture at all. The overuse of organic fertilizer by some organic farmer have caused some severe problems such as NO₃ and P₂O₅ accumulation in soil profile showed the potential risk for nitrate and phosphate leaching. In the paper, it is discussed on the the environmental friendly function and the safe vegetable production by european organic agriculture which keeps the internationally recognized basic standards of organic agriculture. Therefore it is strongly recommended that korean organic farmer have to follow the IFOAM basic standard in order to practice the environmental agriculture and produce the safe food. And it is also necessary to introduce to Korea the basic standard of organic agriculture which coincides with IFOAM’s and Codex of FAO/WHO immediately if they really want to practice an organic agriculture in the country.

포유동물배의 미세조작 기술

정길생(Kil Saeng Chung) 건국대학교 유전공학연구소 1984 건국 유전공학지 Vol.1 No.-

Prostaglandin F2 - Alpha 및 Nor - Adrenalin 투여가 유우의 발정주기와 혈중 Steroid Hormone 함량에 미치는 영향

정길생 ( Kil Saeng Chung ) 한국축산학회 1979 한국축산학회지 Vol.21 No.5

This experiment was carried out to develop a new luteolytic reagent and its effective administration technique which could be used for the synchronization of estrus cycle in dairy cattle. Five miligram of prostaglandin F₂x and 2 ㎎ of nor-adrenalin were administrated by way of intravulvosubmucous, respectively, and the induction of estrus and the rates of synchronization were observed. The changes in the concentration of steroid hormones such as progesterone, estradiol-17β and testosterone in blood plasma of dairy cattle prior to and after administration of the reagents were also estimated. The results obtained in this experiment were summarized as follows; 1) Standing estrus was induced in 8 cows out of 10 cows within 2 to 4 days after administration of prostaglandin F 2α injected by way of intravulvosubmucous at 10 days after natural ovulation. 2) Six cows out of 10 cows were resulted in standing estrus within 5 to 6 days after administration of nor-adrenalin via intravulvosubmucous at 10 days after natural ovulation. 3) The concentrations of steroid hormens in blood plasma of dairy cattle responded to the administration of prostaglandin F2α and nor-adrenalin were determined. The Plasma progesterone levels had fallen from 7.615±0.81ng/㎖ and 6.242±182ng/㎖ to 2.839±0.341ng/㎖ and 1.583±0.520 ng/㎖, respectively, within 6 hoots after administration of prostaglandin F2α and noradrenalin and then declined to a baseline level within 24 hours. 4) The concentration of plasma estradiol-17β rose to a peak level, 55.7±13.19 pg/㎖, within 3 days after injection of prostaglandin F2α and 60.3±8.15 pg/㎖ within 5 days after injection of nor-adrenalin, respectively, and the estrus were induced at the time when estradiol-17β levels pose to the peak, respectively. 5) The peak levels of estradiol-17β during the estrus induced by the administration of prostaglandin F2α or nor-adrenalin were lower significantly than those of estradiol-17β during the natural estrus, respectively. 6) Testosterone concentration increased during the 3 days preceding estrus and attained peak values, 59.4±9.73pg/㎖ and 55.8±6.14pg/㎖, respectively, 48 hour before the onset of estrus induced by prostaglandin F2X or nor-adrenalin. 7j Progesterone concentrations in blood plasma of dairy cattle not responded to the administration of prostaglandin or nor-adrenalin were also fallen sharply within 6 hours after administration of the reagents but no levels below 1.0 ng/㎖ were observed. 8) From the results above mentioned it could be concluded that nor-adrenalin was as effective reagent as prostaglandin F2α for the synchronization of estrus cycle in dairy cattle and that intravulvosubmucous injection method was the most effective technique for the administration of the reagents.