Comparison of controlled ovarian stimulation outcomes for fertility preservation in women with breast cancer according to KI-67, histologic grade, pathologic type, and cancer stage

( Eun Ji Oh ),( Yeon Hee Hong ),( Seul Ki Kim ),( Jung Ryeol Lee ),( Byung Chul Jee ),( Chang Suk Suh ),( Seok Hyun Kim ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: This study aims to analyze that specific prognostic factors such as Ki-67 value, histologic grade, pathology type, and cancer stage can affect controlled ovarian stimulation outcomes(COS) for fertility preservation in women with breast cancer. Methods: This retrospective cohort study included 92 patients with breast cancer aged 21-44 who underwent COS from August 2012 to April 2020, excluding patients with previous gonadotoxic therapy history. All COS cycles were conducted letrozole-combined random start GnRH antagonist protocol. COS outcomes were compared according to prognostic factors;Ki-67 (< 30% vs. ≥ 30%), histologic grade (low vs. high), and pathologic type (intraductal carcinoma(IDC) vs. triple-negative breast cancer(TNBC)), and cancer stage (early (I, II) vs. advanced (III, IV)). Multivariate analysis was also conducted to find any parameter that can impact over 10 mature oocytes acquisition. Results: Among the 92 patients, 42 were Ki-67≥30% compared to 49 of Ki-67<30%. The number of oocytes, and initial mature oocytes were comparable between Ki-67<30% and ≥30 group. The maturation rate was significantly higher in Ki-67≥30% group than Ki-67<30% group (57.4±36.8% vs. 44.8±28.3%, p=0.041). The same results were drawn when divided according to IDC or TNBC. The maturation rate was significantly higher in TNBC than the IDC group (73.0±17.6% vs. 47.0±28.1% p=0.001). However, the histologic grade or stage did not show any difference in COS outcomes between high and low grade or early and advanced stages. With multivariate analysis, age and AMH are parameters associated with the acquisition of over 10 mature oocytes (OR 0.863, 95% CI [0.755-0.987], OR 1.408, 95% CI [1.145-1.732], respectively). Conclusion: Either Ki-67 or pathologic type can be a potential reliable marker for predicting COS outcome, especially maturation rate. Further study with larger sample size with specific conditions is necessary to clarify the correlation between breast cancer prognostic factors and COS outcomes.

Expression of Wnt Target Genes in Solid Pseudopapillary Tumor of the Pancreas: A Pilot Study

Kang, Chang Moo,Kim, Hyun Ki,Kim, Hoguen,Choi, Gi Hong,Kim, Kyung Sik,Choi, Jin Sub,Lee, Woo Jung Lippincott Williams Wilkins, Inc. 2009 PANCREAS Vol.38 No.2

OBJECTIVES:: Solid pseudopapillary tumor (SPT) of the pancreas is very rare. This study was performed to analyze the expression of Wnt signal target genes (matrix metalloproteinase-7 [MMP-7], cyclin-D1, and c-myc) and Ki-67 in resected SPTs to determine their clinicopathologic characteristics according to their expression. METHODS:: From January 1995 to December 2005, 23 patients underwent pancreatic resections for SPT of the pancreas. Among 23 formalin-fixed, paraffin-embedded tissues, 12 were evaluated as a pilot study. Immunohistochemistry was performed using various detection and antigen retrieval methods to detect MMP-7, cyclin-D1, c-myc, and Ki-67. The expression of Wnt target genes was correlated with clinicopathologic features of the patients. RESULTS:: Solid pseudopapillary tumors of the pancreas always showed cytoplasmic/nuclear accumulation of &bgr;-catenin, frequent expression of cyclin-D1, and low proliferation index. MMP-7, cyclin-D1, c-myc, and Ki-67 were not correlated with microscopic features suggesting malignant potential (P > 0.05). Tumor size was closely related to microscopic features of malignant potential and apparently has an inverse relationship with the expression of cyclin-D1 and Ki-67 (P < 0.05). CONCLUSIONS:: Low proliferative index and associated MMP-7 expression may cause an unpredictable clinical course in this tumor. Subtle changes in the intracellular environment, not pathologic (morphologic) changes, may elucidate the unpredictable clinical course of this tumor.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

운동선수의 임장불안에 대한 연구

홍관이,이광재,김두경,부기원,노성규,한상준,박기동,오수일,문병용,이철규,박장평,엄기진,박남환 江原大學校附設 體育科學硏究所 1984 江原大學校附設體育科學硏究所論文集 Vol.- No.9

This study is designed to grasp the elements and situations of stage fright in order to propose a part of ways to cope with it. We make 511 top-class high school players, male and female, in the central districts (Seoul, Inchon, Kyungki, Chungbuk, and Kangweon Province) on object of questionnaire, and get the following results. Through players generally have all-round characters they show weakness in the superiority and emotional stability while showing strength in the sonformity. This fact comes to be related to the elements which cause stage fright. Physiological changes also have many effects on the symptom of stage fright. Especially, stage fright increases when players show sensitive reaction to the self-consciousness of final consequence which comes from the tension or uneasiness of sympathetic nerve, Thus, it turns out that players make use of reducing psychological burden by physical exercise or adjusting the level of demanded result in order to release the stage fright.

ISOLATION AND CHARACTERIZATION OF MOLECULAR MARKER FOR SEVERAL RACES OF PYRICULARIA ORYZAE USING RANDOM AMPLIFIED POLYMORPHIC DNA

Hong, Sun-Min,Bae, Dong-Won,Kim, Mee-Hyang,Shim, Chang-Ki,Kang, Kyu-Young,Kim, Nam-Soo,Kim, Hee-Kyu Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.

Primer 155(GCTGGCGCTG) was the most useful primer with distinctive polymorphic DNA pattern to distinguish between KI races and KJ races. Primer 173(CAGGCGGCGT) showed polymorphic DNA pattern to distinguish isolates of KI-100 race. The specific DNA fragment of 0.6Kb, generated from 88-62 of KJ-201 race by RAPD with primer 412)TGCGCCGCTG), was promising as a molecular marker to identify the isolate.

大豆에 있어서 Gamma ray 感受性의 品種間 差異

Ki Chang Hong(洪基昶),Sin Won Kang(姜信元) 한국육종학회 1970 한국육종학회지 Vol.2 No.1

大豆 放射線 育種의 한가지 基礎硏究로서 Gamma ray에 對한 感受性의 品種間 差에 對하여 試驗한 바 그 結果를 要約하면 다음과 같다. (1) Gamma ray 照射에 따라서 高線量에서는 發芽率이 大體로 抑制傾向을 보인 것이 많았다. Hill 만을 全線量(15~60kR)에서 發芽率이 增大되었다. (2) 全品種이 Gamma ray 照射에 依해 發芽期間이 延長되었으나 忠北白과 益山만은 低線量에서는 도리어 短縮되는 傾向을 表示하고 있다. (3) 上胚軸長은 全體的으로 減少 傾向을 보였으나 忠北白과 益山은 低線量에서는 도리어 增大傾向을 보였다. (4) 下胚軸長도 全體的으로 減少 傾向을 보였으나 Hill만은 全線量에서, 益山을 除한 全品種이 15kR에서 增大傾向을 보였다. (5) 葉面指數는 全品種이 全線量에 增大되었다. (6) 莖長은 益山과 Hill에서는 15~35kR 에서 增大되고 45kR 以上에서 減少되었으며 基他 品種은 全線量에서 減少되었다. (7) 葉長은 거의 全照射區에서 減少되었으나 金豆益山 및 Hill의 15 kR 增大되는 傾向이 있었다. (8) Gamma ray 照射에 依하여 12種類 以上의 畸型葉이 相當한 頻度로 出現하였다. (9) 初期부터 여러個의 側枝가 一齊히 發育하여 正常個體보다 生育이 極히 旺盛한 異常 個體도 發見되었다. In order to find out the varietal differences of radiation sensitivity in five soybean varieties, Chung-buk-baek, Yuk-u #3, Keum-du, lk-san, Chang-dan-baek-mok and Hill were treated with 15, 25, 35, 45, and 60 kR of gamma ray. The results gained are summarized as follows; (1) The germination percentage decreased with an increase of gamma ray dosage except the slight stimulation at lower doses in most of the varieties. But Hill was stimulated from all doses. (2) The length of time for germination was prolonged by the gamma ray irradiation in four varieties, while Chung-buk-back and Ik-san were shortened in low dose. (3) The length of epicotyl was reduced in four varieties, the other varieties, Chungbuk-baek and Ik-san were increased in lower doses. (4) The length of hypocotyl was reduced with increasing dosage in five varieties except Hill, which was increased with all doses, while all varieties, except Ik-san were increased at 15 kR. (5) The leaf index was increased proportionally to high dosage. (6) The length of stem was increased between 15 kR and 35 kR in Ik-san and Hill, but reduced in all doses in the other four varieties. (7) in general, the length of leaf was decreased from all doses, but it was increased at the 15 kR level in Keum-do, Ik-san and Hill. (8) More than twelve kinds of abnormal leaf types resulted from various dose levels. (9) More vigorous plants of a bushy type were found in the experiment.

A Numerical Analysis of Fluid Flow,Heat Transfer and Solidification in the Bending-Type Square Billet Continuous Casting Process

Hong, Ki-Ha,Kim, Chang-Soo,Cha, Pil-Ryung,Yoon, Jong-Kyu 대한금속학회 2002 METALS AND MATERIALS International Vol.8 No.1

A numerical modeling system was developed which can simulate the transport phenomena of a bending type square billet continuous casting process. Fluid flow and heat transfer were analyzed with a 3-dimensional finite volume method (FVM) with the aid of an effective heat capacity algorithm for the solidification. For a complex geometry of the bending type billet caster, a body-fitted-coordinate (BFC) system was employed. The bent structure of the caster allows a recirculating flow to develop in the upper and outer-radius region and the main stream to shift toward inner radius. This causes the thinner solid shell in the inner radius region than in the outer one. Besides standard operation conditions, we have analyzed the results when casting speed, caster shape, and tundish superheat changes. Lower casting speed makes the solid shell thicker by reducing heat flux from the mold. In the vertical caster, solid shell thickness are more uniform than that in the bending-type in entire region. When superheat increases by 5℃, solid shell thickness at the mold exit becomes thinner by 1 mm.

활동적 생활습관과 복합운동이 인슐린 저항성에 미치는 영향

홍창배(Chang Bae Hong),김기진(Ki Jin Kim) 한국사회체육학회 2007 한국사회체육학회지 Vol.0 No.30

The aim of the current study was to examine the changes of body composition, insulin resistance, blood lipid profiles and inflammatory markers following to spontaneous or controlled exercise participation in Korean adults selected from the general population. Subjects with previous diagnosis of diabetes were divided into 2 groups based upon spontaneous exercise participation for using a cross-sectional approach as active group (n=16) and non-active group (n=16). Additionally, we analyzed the effects of a 12 week exercise intervention program by the comparison of between exercise group (n=12) and control group (n=12). We measured anthropometric factors (BMI, percent body fat, WHR, and abdominal fat area by CT scanning), BP, blood levels of glucose, lipid profiles, fibrinogen, CRP, leptin, HbA1c, PAI-1, HOMA, OGTT and physical fitness. Independent t-tests were used to assess differences between active and non-active groups. Additionally, 2-way repeated ANOVA for group and time was used to compare the changes after a 12 week exercise intervention program. Significance was accepted at p<0.05. In cross sectional comparison, active group showed significantly (p<0.05) lower in WHR, visceral fat area, VSR, blood levels of TG, HbA1c and CRP than non-active group. Active group had a lower trend for HOMA-IR than non-active group, and showed significantly (p<0.05) lower in glucose response area of OGTT than non-active group. Exercise group showed a significant (p<0.05) decrease in WHR and HOMA-IR whereas control group showed no changes. In these results, we confirmed that continuous and regular exercise participation has a positive effect on insulin resistance with the decrease of WHR, visceral fat area and blood level of TG. We think that the more long-term exercise intervention may be effective improvement on insulin resistance.

폭주과다 및 개선부족 환자에 대한 시기능 훈련 효과

이창선 ( Chang Seon Lee ),김기홍 ( Ki Hong Kim ) 대구가톨릭대학교 자연과학연구소 2012 자연과학연구논문집 Vol.10 No.1

This study was performed to investigate vision training was an effective way to influence fusional vergence and asthenopic symptoms in case of convergence excess and divergence excess. The change was examined weekly at the clinic during the 7 week vision training. According to the results, MISTERY CIRCLE vision training methods improved the binocular vision function and sensation symptoms. For Worth 4 Dot test, Howell test card and fusion reserve test showed fusion rate changes. The study proved that visio training methods for patients with binocular vision was effective for the improvement of the phoria, function and sensation symptoms of these patients.

Effect of Extreme Heatwaves on the Mortality and Cellular Immune Responses of Purplish Bifurcate Mussel Mytilisepta virgata (Wiegmann, 1837) (=Septifer virgatus) in Indoor Mesocosm Experiments

Hyun-Ki Hong,Chang Wan Kim,Jeong-Hwa Kim,Nobuhisa Kajino,Kwang-Sik Choi 제주대학교 해양과학연구소 2021 해양과환경연구소 연구논문집 Vol.45 No.-

In the rocky intertidal environment, the frequency and duration of heatwaves have increased over the last decade, possibly due to global climate change. Heatwaves often result in lethal or sub-lethal disturbances in benthic animals by changing their metabolic activities. In this study, we investigated the impacts of extreme heatwave stress on the hemocyte functions of Mytilisepta virgata and subsequent mortality to gain a better understanding of the potential causes and consequences of mass mortality events in this mussel during summer. We discriminated three types of hemocytes in the hemolymph, granulocytes, hyalinocytes, and blast-like cells, using flow cytometry and revealed that granulocytes were the major hemocyte involved in cellular defensive activities, such as phagocytosis and reactive oxygen species (ROS) production. For the experiment, mussels were exposed to a 40◦C air temperature for 12 h per day over 5 days under laboratory conditions as a simulated semi-diurnal tidal cycle. Mortality began to occur within 3 days after beginning the experiment, and all mussels had died by the end of the experiment. Flow cytometry indicated that the mussels exposed to high air temperatures produced significantly more ROS than did the control mussels within 2 days after the onset of the experiment, which may have caused oxidative stress. Such high levels of ROS in the hemolymph increased DNA damage in hemocytes after 3 days of exposure and decreased the phagocytosis of hemocytes 4 days after the experiment began. The observed mortality and decline in immune capacity suggested that an extreme heat event occurring in the rocky intertidal ecosystem during summer could exert sublethal to lethal impacts on macrobenthic animals.