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Ping Hongyan,Wang Degang,Fu Maxian,Chen Kaihong,Zhang Jiuhong,Li Ke,Jiang Xuewu,Duan Shouxing,Zhang Xuan 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.4
Background Diethylstilbestrol (DES) has been shown to disrupt the morphology and proliferation of gubernaculum testis cells. Objective This study aimed to elucidate the mechanism underlying DES induced damage to gubernaculum testis based on proteome analysis. Results Neonatal mice were exposed to DES or control vehicle and the gonads were collected for isobaric tags for relative and absolute quantitation based proteomics analysis. We found that at the early and late stages of gubernaculum development, treatment with different concentrations of DES upregulated the expression of proteins which were generally involved in several function categories including single-organism process, cellular process, binding, catalytic activity, cell part and organelle. KEGG pathway analysis showed that cardiac muscle contraction, oxidative phosphorylation, calcium signaling and cGMP-PKG signaling pathway were the most dysregulated pathways associated with DES in the late stage of gubernaculum development, while steroid biosynthesis was the only enriched pathway in the early stage of gubernaculum development. Conclusion Proteomics profi ling and functional analysis reveal new clues on how DES interferes with cellular processes and disrupts the development of gubernaculum. These findings have potential applications in the treatment of genitourinary diseases such as cryptorchidism.
Fatigue behavior of concrete beams reinforced with HRBF500 steel bars
Ke Li,Xin-Ling Wang,Shuang-Yin Cao,Qing-Ping Chen 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.2
The purpose of this study was to investigate experimentally the fatigue performance of reinforced concrete (RC) beams with hot-rolled ribbed fine-grained steel bars of yielding strength 500MPa (HRBF500). Three rectangular and three T-section RC beams with HRBF500 bars were constructed and tested under static and constant-amplitude cyclic loading. Prior to the application of repeated loading, all beams were initially cracked under static loading. The major test variables were the steel ratio, crosssectionalshape and stress range. The stress evolution of HRBF500 bars, the information about crack growth and the deflection developments of test beams were presented and analyzed. Rapid increases in deflections and tension steel stress occured in the early stages of fatigue loading, and were followed by a relatively stable period. Test results indicate that, the concrete beams reinforced with appropriate amount of HRBF500 bars can survive 2.5 million cycles of constant-amplitude cyclic loading with no apparent signs of damage, on condition that the initial extreme tensile stress in HRBF500 steel bars was controlled less than 150 MPa. It was also found that, the initial extreme tension steel stress, stress range, and steel ratio were the main factors that affected the fatigue properties of RC beams with HRBF500 bars, whose effects on fatigueproperties were fully discussed in this paper, while the cross-sectional shape had no significant influence in fatigue properties. The results provide important guidance for the fatigue design of concrete beams reinforced with HRBF500 steel bars.
Ke-quan Chen,Zhen Wang,Wen Xiao,Alie Zhang,Hanxiao Ying,Ping-Kai Ouyang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.10
Actinobacillus succinogenes NJ113 is capable of microaerobic fermentation, which offers the possibility of a novel type of pyruvic acid production. A dissolved oxygen environment with stirring at 300 rpm was a key factor in the fermentative production of a maximum concentration of pyruvic acid. Potassium carbonate (K2CO3) was found to have a role in promoting pyruvic acid production, influencing the concentration of pyruvic acid and production of the by-product succinic acid. The final titer of pyruvic acid production was 36.8±0.1 g L−1 with an overall yield of 0.639±0.056 g g−1 glucose and 3.12±0.03mmol g−1 dry cell weight h−1. Significance and impact of the study: This study is the first to illustrate the advantage of using Actinobacillus succinogenes NJ113 with no genetic modification under microaerobic conditions for the production of pyruvic acid.
( Ping Lu ),( Ke Jiang ),( Ya-qiao Hao ),( Wan-ying Chu ),( Yu-dong Xu ),( Jia-yao Yang ),( Jia-le Chen ),( Guo-hong Zeng ),( Zhou-hang Gu ),( Hong-xin Zhao ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9
Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.
Fatigue behavior of concrete beams reinforced with HRBF500 steel bars
Li, Ke,Wang, Xin-Ling,Cao, Shuang-Yin,Chen, Qing-Ping Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.2
The purpose of this study was to investigate experimentally the fatigue performance of reinforced concrete (RC) beams with hot-rolled ribbed fine-grained steel bars of yielding strength 500MPa (HRBF500). Three rectangular and three T-section RC beams with HRBF500 bars were constructed and tested under static and constant-amplitude cyclic loading. Prior to the application of repeated loading, all beams were initially cracked under static loading. The major test variables were the steel ratio, cross-sectional shape and stress range. The stress evolution of HRBF500 bars, the information about crack growth and the deflection developments of test beams were presented and analyzed. Rapid increases in deflections and tension steel stress occured in the early stages of fatigue loading, and were followed by a relatively stable period. Test results indicate that, the concrete beams reinforced with appropriate amount of HRBF500 bars can survive 2.5 million cycles of constant-amplitude cyclic loading with no apparent signs of damage, on condition that the initial extreme tensile stress in HRBF500 steel bars was controlled less than 150 MPa. It was also found that, the initial extreme tension steel stress, stress range, and steel ratio were the main factors that affected the fatigue properties of RC beams with HRBF500 bars, whose effects on fatigue properties were fully discussed in this paper, while the cross-sectional shape had no significant influence in fatigue properties. The results provide important guidance for the fatigue design of concrete beams reinforced with HRBF500 steel bars.
Shun Weng,Ke Gao,Zhi-Dan Chen,Hong Ping Zhu,Li-Ying Wu 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.1
The effectiveness of system identification, damage detection, condition assessment and other structural analyses relies heavily on the accuracy and reliability of the measured data in structural health monitoring (SHM) systems. However, data anomalies often occur in SHM systems, leading to inaccurate and untrustworthy analysis results. Therefore, anomalies in the raw data should be detected and cleansed before further analysis. Previous studies on data anomaly detection mainly focused on just single type of data anomaly for denoising or removing outliers, meanwhile, the existing methods of detecting multiple data anomalies are usually time consuming. For these reasons, recognising multiple anomaly patterns for real-time alarm and analysis in field monitoring remains a challenge. Aiming to achieve an efficient and accurate detection for multi-type data anomalies for field SHM, this study proposes a pattern-recognition-based data anomaly detection method that mainly consists of three steps: the feature extraction from the long time-series data samples, the training of a pattern recognition neural network (PRNN) using the features and finally the detection of data anomalies. The feature extraction step remarkably reduces the time cost of the network training, making the detection process very fast. The performance of the proposed method is verified on the basis of the SHM data of two practical long-span bridges. Results indicate that the proposed method recognises multiple data anomalies with very high accuracy and low calculation cost, demonstrating its applicability in field monitoring.
( Peng Lii ),( Ke Ping Chen ),( Hua Jun Yang ),( Qin Yao ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.15 No.2
As the genome of B.mori is available in GenBank and the EST database of B.mori is expanding, identification of novel genes of B.mori is conceivable by data-mining techniques. We used the in silico cloning method to get the vacuolar-type H(+)-ATP synthetase (V-ATPase) c subunit (16 kDa proteolipid subunit) gene of B.mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and sequencing. The V-ATPase c subunit cDNA contains a 468bp ORF. The ORF encoded a 155-residue protein that showed extensive homology with V-ATPase c subunits from other 15 species and contained four membrane-spanning helices. Tissue expression pattern analysis revealed that V-ATPase c expressed strongly in Mal-pighian tubules, not in fat body. This gene has been registered in GenBank under the accession number EU082222.
Weighted FP-Tree Mining Algorithms for Conversion Time Data Flow
Xiao-jun Chen,Jia Ke,Qian-qian Zhang,Xin-ping Song,Xiao-ming Jiang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.1
The data distribution in the data streams usually changes dynamically with time. Traditional mining algorithms based on transaction are difficult to establish the correlation between time characteristics and relationship features, thus making the results inaccurate. By analyzing the problems in the processing of time data stream, we put forward the concept of time gap degrees and design a mining algorithms based on weighted FP-Tree. We introduce the concept of FP-Tree node weights to transform the time data dynamically and excavate the data stream association rules. The experiments performed on the actual data set show that the algorithm can improve the recall and precision while consumes comparable computational time.