광합성세균 Phodospirillum rubrum의 무기질소원의 이용

강봉조,오덕철,이동헌 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.2

Rhodospirillum rubrum, a photosynthetic bacterium, was tested for the utilization of inorganic nitrogen sources such as NH₄Cl, NaNO₂ and NaNO₃. The cell growth was most abundant with NH₄Cl among three nitrogen sources regardless of aerobic or anaerobic condition. With usable nitrogen sources, the cell growth was always greater in anaerobic condition than aerobic condition. R. rubrum used NH₄Cl and NaNO₃, but not NaNO₂ as nitrogen source in anaerobic condition. But in aerobic condition, R. rubrum used all nitrogen sources. The sonsumption of total nitrogen by R. rubrum used was highest in the NH₄Cl medium among three kinds of media regardless of culture conditions. The pH of media were increased according to the cell growth. The production of nitrite from nitrate in the NaNO₃ medium was much greater in anaerobic condition than aerobic condition.

백색부후균 전처리에 의한 목재 칩의 Biopulping

강규영,김형진,양봉숙,조병묵,오정수 동국대학교 생명자원과학대학 연습림 2000 연습림논문집 Vol.- No.6

Pulping process is to mainly obtain lignocellulosic fibers for papermaking from wood resources. Three types of pulping method have been basically employed, such as chemical pulping, mechanical pulping, and their combination methods. Biopulping technique has been mainly applied in mechanical pulping by pretreatment of white-rot fungi into the ultrastructure of wood cell wall. The main purpose of biopulping is to improve the pulp qualities, paper properties, and to reduce the energy costs and environmental impact, compared to traditional pulping systems. Nowadays, biopulping is frequently tried in chemical pulping operation for environmental concept, fiber modification, improvement of physical properties of paper and economic savings. The pretreatment of white-rot fungi prior to chemical pulping could affect the swelling and softening of wood cell walls by the behaviors of modification and depolymerization of ligin compounds. This study was carried out to investigate the effectiveness of biological treatment to wood chips by white-rot fungus and its structural change of lignocellulosic fiber via kraft pulping. A rotary bioreactor was applied for pretreatment of biological action. The treatment of white-rot fungus was shown in weight loss of chips. After bio-kraft pulping, kappa number and total yield of pulps were measured, and reduced. Freeness(CSF) values were also decreased. Whereas brightness values were increased, compared to untreated control. The physical strength of handsheets was shown a slight increase in tensile, tear, burst, and folding endurance, with bio-treatments.

제주연안 갯녹음(백화) 지역의 해수에 분포하는 세균군의 분자생물학적 분석

강봉조,김미란,윤병준,이동헌,오덕철,강형일 濟州大學校 基礎科學硏究所 2002 基礎科學硏究 Vol.15 No.2

본 연구에서는 갯녹음(백화)현상이 세균생태계와 어떤 관련이 있는지에 대한 기초자료 및 정보를 얻기 위하여 갯녹음 현상이 일어난 제주도 성산과 강정지역 연안의 해수에 존재하는 세균군을 16S rRNA 증폭기법을 이용하여 조사하였다. 강정지역에서는 Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacter, Sulfitobacter, Roseobacter 등 다양한 종류의 세균이 분포되어 있었으며, Alcanivorax가 20%의 빈도로 가장 많이 나타났다. 반면, 성산 지역에서는 Pseudomonas속 균주가 우점종으로서 존재하였으며, Pseudomonas tolaasii(혹은 Pseudomonas corrugata)와 유연관계가 가까운 세균은 44%, Pseudomonas mandeli와 가까운 세균이 24%, Verrucomicrobiales와 가까운 세균은 4%, 기타 동정되지 않은 세균은 세 group으로 구분되었으며, 각각 8%, 8%, 12%를 차지하여, 두 곳에 분포되어 있는 세균군상이 상당한 차이점이 있음을 확인하였다. 갯녹음 지역인 강정과 성산 해수의 8월(표품 채집시기)의 수온은 27℃-27.5℃, 염분의 농도는 30.24-30.60%, pH는 8.23-8.36, 용존산소량(DO)은 각각 7.20-7.28로, 두 지역에서 매우 비슷한 것으로 조사된 바 있는데, 이는 수온이나 염분의 농도, 또는 pH보다는 다른 원인에 의하여 두 갯녹음 지역에 분포하는 세균군의 차이를 가져왔음을 제시해 주었다. In this study, the bacterial communities distributed in sea water of the whitening areas of Gangjeong and Seongsan, Jeju-do have been analyzed using the PCR amplification of 16S rRNA to obtain fundamental data and information on relationship of the whitening phenomenon and microbial ecosystem. In Gangjeong, diverse bacteria such as Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodovulum, Silicibacier, Sulfitobacter, and Roseobacier have been found, and Alcanivorax was the most abundant clone. The most abundant clone from Seongsan was Pseudoinonas, of which Pseudomonastolaasii and Pseudonionas mandeli were most abundantly occurred in the frequency of approx 44% and 24%, respectively. Approx 4% of the bacterial clones closest to firruconiicrobiales and other unidentified clones were also found in Seongsan, suggesting there is a great discrepancy between bacterial communities from the whitening areas of Seongsan and Gangjeong. The mean tem- perature, chlorine concentration, pH, and dissolved oxygen (DO) of the sea water of Gangjeong and Seongsan in August of 2001 (sampling period) was 27℃-27.5℃, 30.24-30.60%, pH 8.23-8.36,7.20-7.28 ㎎/ℓ, suggesting other environmental factors except for the factors mentioned above might result in difference of bacterial communities distributed in both areas.

만성 남자 정신분열병 환자의 병식 정도와 기질 및 성격 유형과의 관계

강여화,김봉조,손진욱 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 남자 만성 정신분열병 입원 환자에서의 병식 정도와 기질 및 성격유형간의 관련성을 규명하고자 한다. 부가적으로 병식 정도에 따른 사회 인구학적 변인 및 질병 연관 변인의 차이를 알아보고자 한다. 연구방법 병식 평가를 위해 McEvoy등이 고안한 '병식과 치료태도에 대한 설문(ITAQ : Insight and Treat-ment Attitudes Questionnaire)'과 Amador 등이 고안한 병식 평가 검사(SUMD : Scale to As-sess Unawareness of Mental Disorder)를 이용한 구조화된 면담을 시행하였다. 다음, Cloninger의 기질-성격 철도(TCI : Temperament and Character inventory) 한국판을 이용하여 기질 및 성격특성을 측정하였다. 또한 병록지 검토와 면담을 통해 인구학적 변인 및 질병 연관 변인에 관한 자료를 얻었다. DSM-Ⅳ 진단기준에 의한 최종 분석의 대상이 된 환자는 113명이었다. 병식의 정도에 따라 병식이 높은 군(중간값 11점 이상)과 낮은 군(11점 이하)으로 양분하였다. 연구결과 1) ITAQ 병식 점수에 따른 양군의 비교에서 통계적으로 유의한 차이를 보인 척도는 '새로운 것을 추구하는 성향'이었다. 2) ITAQ 병식 점수와 위험 회피적 성향 사이에서 유의한 상관관계가 있었고, '새로운 것을 추구하는 성향'과는 유의한 음성적 상관관계를 보였다. 하지만, 후천적으로 결정되는 성격 척도 사이에서는 통계적 유의성이 없었다. 3) 질병 연관 변인들 중 첫 발병 나이, 입원 형태, 유병기간, 현재 입원기간, 약물투여량은 양군간에 통계적으로 유의한 차이가 있었다. 결 론 본 연구 결과는 새로움 추구 성향이 높고 위험 회피 성향이 낮을수록 낮은 병식을 가질 수 있다는 병식 정도에 대한 기질특성의 영향을 시사한다. 그러므로 병식의 평가 단계에서 기질 유형을 파악하는 것이 임상적으로 의미가 있을 것이다. 앞으로 정신분열병 환자의 기질 및 성격 유형에 관한 지속적인 연구가 필요할 것이다. Objectives : This study was conducted to investigate the relationship of insight level with temperament and character pattern in chronic male schizophrenics. In addition, we tried to examine demographic variables and illness-related variables which may affect the level of insight. Methods : To assess insight level by psychiatrists, Insight and Treatment Attitudes Question-naire(ITAQ), Scale to Assess Unawareness of Mental Disorder(SUMD) were applied. Tem-perament and character patterns were evaluated by the Korean version of Cloninger's self-rated Temperament and Character Inventory(TCI). The authors investigated the correlations between insight level, and scores of temperament and character dimensions. Data on the demographic and illness-related variabres for the subjects were gathered from hospital records and clinical interviews. A total of 113 patients with schizophrenia according to DSM-Ⅳ criteria was analyzed. The insight level was dichotomized into good(≥median value of 11) and poor insight group(<11). Results : 1) There was a significant difference between good insightgroup and poor insight group in novelty seeking dimension in TCI. 2) There was a significant negative correlation between novelty seeking scores by TCI and insight scores by ITAQ(rs : Spearman correlation coefficient=-0.20, p<0.05). 3) Significant differences between good and poor insight groups were identified in diverse clinical variables such as age at onset of illness, mode of hospitalization, duration of illness, current length of stay, and dosage of drug. Conclusion : The results suggest that high scores in the temperament dimension of novelty seeking and low scores in the temperament dimension of harm avoidance may influence poor insight in patients with chronic male schizophrenia. Therefore it is clinically valuable for us to assess the pattern of temperament at the stage of insight evaluation. The authors suggest that further studies on temperament and character in schizophrenia is needed.

TDI계 수분산성 폴리우레탄 접착제의 특성에 Diisocyanate의 구조가 미치는 영향

강승구,송봉근,이재흥,박찬조,류해일 한국공업화학회 2003 공업화학 Vol.14 No.3

수년 전부터 많은 연구자들에 의해 폴리우레탄의 수용화, 수분산화의 연구가 수행되어 왔다. 그럼에도 불구하고 폴리우레탄의 특성에 디이소시아네이트가 구조에 미치는 영향에 대한 연구는 많이 진행되지 않았고 또한 미흡한 상태이다. 따라서 번 연구에서는 디이소시아네이트의 형태와 함량 변화에 따른 폴리우레탄의 특성을 연구하였다. 폴리우레1탄 수분산체(PUD)는 poly(tetramethylent ether) glycol (PTMG), isophorone diisocyanate (IPDI), toluene 2,4-diisocyanate (TDI), dimethylol propionic acid (DMPA), trethylamine (TEA) 등을 사용하여 제조하였다. 제조한 폴리우레탄 수분산체(PUD)의 구조를 FT-IR을 이용하여 확인하였다. 폴리우레탄 수분산체의 특성은 DSC, gel permeation chromatography (GPC), particle analyzer, universal testing machine (UTM) 등을 사용하여 측정하였다. 폴리우레탄 수분산체(PHD)에서 입자크기는 IPDI 함량이 증가할수록 작아지는 경향을 나타내었으며, 유리전이온도(Tg)는 IPDI 함량이 증가할수록 조금씩 낮아지는 경향을 나타내었다. 또한, 폴리우레탄 수분산체(PUD)의 접착세기 변화에서는 [NCO]/[OH] 몰비를 1.2로 유지되도록 하면서 합성에 관여하는 [NCO] 양에 해당하는 [TDI]/[IPDI] 몰비를 변화시켜 얻은 폴리우레탄 수분산체의 접착세기는 [TDI][IPDI] 몰비가 50/50일때 좋은 집착세기를 나타내었다. 또한 [NCO]/[OH] 몰비를 1.6으로 일정하게 유지되도록 하고 [TDI]/[IPDI] 몰비를 변화시켜 얻은 폴리우레탄 수분산체의 접착세기는 [TDI]/[IPDI] 몰비가 75/25일 때 좋은 접착세기를 나타내었다. Aqueous polyurethane dispersions (PUD) has been investigated by many researchers for a long time. However, relatively little systematic work has been reported, in detail, on the effect of structure of the diisocyanate on the properties of polyurethane. This work describes the properties of polyurethane in relation to the variation of diisocyanate type and contents. Aqueous polyurethane dispersions (PUD) were prepared from the poly(tetramethylene ether) glycol (PTMG), toluene 2,4-diisocyanate (TDI), isophorone diisocyanate (IPDI), dimethyol propionic acid (DMPA), and triethylamine (TEA). Differential scanning calorimetry (DSC), FT-IR, GPC, universal testing machine (UTM), and particle analyzer were utilized to characterize the physical and mechanical properties of PUD according to diisocyanate type and contents. As the content of IPDI in PUD increased, the particle size decreased. The glass transition temperatures (Tg) of PUT were shifted to a lower temperature as IPDI conent increased. When [NCO]/[OH] mole ratio was 1.2, the adhesion force of PUD shown the best result at [TDI]/[IPDI] mole ratio of 50/50. And, at [NCO]/[OH] mole ratio of 1.2, the adhesion force of PUD was the best when [TDIl/[IPDI] mole ratio was 75/25.

효소 전처리 BCTMP 시트의 노화 거동

양봉숙,김형진,강규영,조병묵,오정수 동국대학교 생명자원과학대학 연습림 2000 연습림논문집 Vol.- No.6

It was generally known that the aging treatment of paper is accompanied with the reductions in physical and optical properties of individual fibers and sheet. In particular, the paper prepared with mechanical pulps occurs the yellowing of sheet, during aging, due to the absorption of light or temperature, and finally has limitation in widely applicable functions of paper. The aging behaviors of paper can be discussed with three groups of factor. One is considered to external factors, like heat, light and moisture. Another is commented with environmental factors, like oxygen, nitrogen, sulfur, and other air pollutant materials. The other is explained by internal factors, like pulp types, lignin contents, and so on. In this paper, the aging behaviors of paper prepared with BCTMP were studied in order to know how to affect in physical and optical properties of paper. For modification of fibers properties, a commercial enzyme was pretreated in the slurry of BCTMP, before handsheet-making. Sheets were aged by accelerated heat treatment. The comparisons were carried out in mechanical strength properties and color reversions. Aging treatments were resulted in higher brightness reductions, in enzyme pretreated paper.

공작기계 주축용 베어링 결함 자동진단시스템의 개발

정의식,강형모,류봉조,임경빈,고준빈,박수규 한밭대학교 산업과학기술연구소 1995 논문집 Vol.3 No.-

This paper deal with a method of detecting localized defects on taper roller bearing in main spindle of machine tool system. The statistical parameters in time-domain signal processing techniques have been calculated to extract useful features from bearing vibration signals. Emloying these features, the statiscical parameters have been established to detect defects on the rollers of bearings, respectively, and used by the input feature of an artificial neural nerwork. As a result that the proposed bearing defects monitoring system could be successfully performed by using the statistical parameters of acceleration signals.

고성능 마이크로프로세서에서 데이터 값 예측기 개발에 관한 연구

박희룡 ; 추봉조 ; 이강한 김천대학교 2000 김천대학교 논문집 Vol.21 No.-

In order to achieve high performance by exploiting instruction level parallelism aggressively in wide-issue superscalar processors, it is necessary to overcome the limitation imposed by control depen-dences and data dependences which prevent instructions from exe-cuting parallel. The value prediction is a technique that breaks datadependences by predicting the outcome of an instruction and executes speculatively its data dependent instruction based on the predicted outcome. In this paper, an extended hybrid-data-value prediction scheme with dynamic classification mechanism is pro-posed. We design a hybrid predictor by combining the last-value predictor, a stride-value predictor and a two-level-value predictor. The choice of a predictor for each instruction is determined by a dynamic classification mechanism. This makes each data predictor utilized more efficiently than the extended hybrid-data-value pre-dictor mechanism. In order to show performance improvements of our scheme, we have simulated the SPECint95 benchmark set by using execution-driven simulator. The results show that the pro-posed scheme effect reduce of 33.3% 45% hardware cost and about 16% prediction accuracy improvements comparing with the conven tional hybrid data value prediction scheme and it also effects the two-level-data value prediction scheme.

사후분해 시체에 대한 법의학적 접근

최영식,이상용,김유훈,조갑래,이봉우,양경무,정낙은,서중석,이한영,이원태,강현욱 大韓法醫學會 2000 대한법의학회지 Vol.24 No.2

Disposal of a homicide victim by dismemberment is rare, but individual cases are on record in most major medicolegal departments. Recognition of postmortem mutilation may be of importance in the interpretation of certain murders committed by sexual perverts and other mentally deranged individuals and sometimes performed for the sole reason of easier disposal of the body. Postmortem dismemberment is usually readily recognizable as such; The edges of the injuries are dry and lack evidence of bleeding. The joints may be disarticulated without fracture, or the use of an axe or saw may be evident from examination of bones. Parallel horizontal or oblique furrows in the bone surface are caused by skipping of the saw prior to establishing depth. Such patterns on the bone may assist in identifying the particular saw involved. So we report 25 dismembered corpses that autopsied in National Institute of Scientific Investigation. This paper can help in attempting to establish not only the first criminal investigation steps but also the medicolegal approach methods in unidentified and dismembered deaths.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.