The Influence of PMMA Bone Cements on the Osteoblasts and Osteoclasts

( Kai-chen Hu ),( Tsung-yan Tsai ),( Chu-wen Shao ),( Hsin-i Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Due to aging, deficiency of vitamins and minerals, a prior accident or injury, the bones in our body become weak and fragile and so may cause serious defect of hard bone tissues. In orthopaedic surgery, Polymethylmethacrylate (PMMA) bone cements are used to refill and remodel bone defect. PMMA bone cements are formulated by mixing the powder (polymer, PMMA) and the liquid (monomer, methyl methacrylate). During the process, the dimethyl-p-toluidine in the liquid activates the benzoyl peroxide catalyst in the powder and this initiates the polymerization of the monomer, which then binds together PMMA granules. However, PMMA bone cements have displayed cytotoxicity to bone tissue in clinic. In present study, we used PMMA with different molecular weights (Mw:1,5000, 12,0000, 35,0000 and 99,6000 dalton) to prepare 4 types of bone cements for studying the effect of molecular weight of polymeric additives. Chemical eluates were extracted from PMMA bone cements and examined for their bioactive effects on 7F2 osteoblast-like cells and Raw 264.7 microphages. Results demonstrated that PMMA bone cements and eluates could inhibit the differentiation and mineralization behavior of 7F2 osteoblast-like cells after the cytotoxicity of PMMA bone cements and elutes were no longer evident. Moreover, bone cements prepared with high molecular weight PMMA (MW: 99,6000 dalton) had less suppressive effect on cell differentiation and mineralization in comparison with others. In Raw 264.7 microphages, PMMA bone cements and eluates can inhibit osteoclast formation but there was no significant difference among 4 types of bone cements. The aim of this research is to investigate the influence of PMMA bone cements on osteoblasts and osteoclasts and we hope these results can provide some useful information for the clinical application of PMMA bone cements.

Dermal Lipogenesis Inhibits Adiponectin Production in Hs68 Fibroblasts

( Kai-chen Hu ),( Tsung-yan Tsai ),( Chu-wen Shao ),( Hsin-i Chang ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Adiponectin is one of the most abundant adipokines from the subcutaneous fat, and regulates multiple activities through endocrine, paracrine, or autocrine mechanisms. However, its expression in adipogenic induced fibroblasts, and the potential role in photoaging has not been determined. Here, human dermal fibroblasts, Hs68, were presented as a cell model of dermal lipogenesis through stimulation of adipogenic differentiation medium (ADM). Similar to other studies in murine pre-adipocyte models (i.e., 3T3-L1), Hs68 fibroblasts showed a tendency to lipogenesis based on lipid accumulation, triglyceride formation, and the expressions of PPAR-γ, lipoprotein lipase (LPL), and FABP4 mRNA. As expected, ADM-treated fibroblasts displayed a reduction on adiponectin expression. Overall, these results suggest that dermal lipogenesis may inhibit the expression of adiponectin.

Effect of Ni2Si Precipitates on Microstructure and Mechanical Properties of Cu–Zn Brasses

Wei Chen,Kai You,Jufang Yin,Ming Chen,Xiaolan Tao,Jiarui Hu,Xiaorong Chen,Qian Lei 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10

Novel ultrahigh strength Ni2Sistrengthened brasses were fabricated, and microstructure and properties were investigatedusing scanning electron microscopy, optical microscopy, transmission electron microscopy, X-ray diffraction analysis, hardnessmeasurement, and electrical conductivity test. After optimal treatments (solid solution treated at 900 ℃ for 2 h, hotrolledby 80%, cold-rolled by 80%, and ageing treated at 300 ℃ for 8 h), the Cu-20Zn-3Ni-0.68Si alloy’s hardness, tensilestrength, yield strength, and electrical conductivity is 270.6 HV, 964 MPa, 933 MPa, 21.6%IACS, and 3.4%, respectively. δ-Ni2Si precipitates formed during the annealing process, which had a crystal orientation relationship with the matrix: (001)m// (001) δ, and [110]m// [100]δ. Quasi-cleavage fracture surfaces with shallow dimples were presented in both the Cu-20Zn-1.5Ni-0.34Si and the Cu-20Zn-3Ni-0.68Si alloy. These findings give a new method to develop new high strength low-costbrass copper materials.

TOSS: Telecom Operations Support Systems for Broadband Services

Chen, Yuan-Kai,Hsu, Chang-Ping,Hu, Chung-Hua,Lin, Rong-Syh,Lin, Yi-Bing,Lyu, Jian-Zhi,Wu, Wudy,Young, Hey-Chyi Korea Information Processing Society 2010 Journal of information processing systems Vol.6 No.1

Due to the convergence of voice, data, and video, today's telecom operators are facing the complexity of service and network management to offer differentiated value-added services that meet customer expectations. Without the operations support of well-developed Business Support System/Operations Support System (BSS/OSS), it is difficult to timely and effectively provide competitive services upon customer request. In this paper, a suite of NGOSS-based Telecom OSS (TOSS) is developed for the support of fulfillment and assurance operations of telecom services and IT services. Four OSS groups, TOSS-P (intelligent service provisioning), TOSS-N (integrated large-scale network management), TOSS-T (trouble handling and resolution), and TOSS-Q (end-to-end service quality management), are organized and integrated following the standard telecom operation processes (i.e., eTOM). We use IPTV and IP-VPN operation scenarios to show how these OSS groups co-work to support daily business operations with the benefits of cost reduction and revenue acceleration.

Dissecting the meteorological and genetic factors affecting rice grain quality in Northeast China

Chen Mojun,Li Zhao,Huang Jie,Yan Yongfeng,Wu Tao,Bian Mingdi,Zhou Jinsong,Wang Yongjun,Lyv Yanjie,Hu Guanghui,Jin Yong-Mei,Huang Kai,Guo Liping,Jiang Wenzhu,Du Xinglin 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background The Northeast Plain of China, which is an important region for the production of high grain quality rice in China. However, the grain quality of the rice produced varies across this region, even for the same cultivar. Objective In order to explore the meteorological factors that have the greatest infuence on quality and the transcriptional level diferences between diferent cultivars and diferent locations at grain flling stage. Methods We grew eight rice cultivars in three locations in Northeast China during two growing seasons (2017 and 2018). We recorded meteorological conditions, including air temperature, air temperature range, and photosynthetically active radiation (PAR) during the grain-flling stage of each cultivar, and analyzed the grain quality of those eight cultivars. Results Across all eight cultivars, meteorological factors had a stronger efect on eating quality than genotype, while genotype had a stronger efect on milling quality. Of the three environmental factors assessed, PAR was signifcantly correlated with the most grain quality traits. Using RNA-sequencing analysis, we identifed 573 environment-specifc DEGs (Diferentially Expressed Genes), and 119 genotype-specifc DEGs; 11 DEGs were responsive to genotype×environment interactions. These DEGs were involved in many key metabolic processes. Conclusion Our results indicated that interactions among environmental factors, especially PAR, afected rice quality in Northeast China. Further analyses of the DEGs identifed herein may provide useful information for future breeding programs aiming to develop high grain quality rice varieties suitable for cultivation across Northeast China.

Trichoderma biodiversity in major ecological systems of China

Kai Dou,Jinxin Gao,Chulong Zhang,Hetong Yang,Xiliang Jiang,Jishun Li,Yaqian Li,Wei Wang,Hongquan Xian,Shigui Li,Yan Liu,Jindong Hu,Jie Chen 한국미생물학회 2019 The journal of microbiology Vol.57 No.8

An investigation of Trichoderma biodiversity involving a large-scale environmental gradient was conducted to understand the Trichoderma distribution in China. A total of 3,999 isolates were isolated from forestry, grassland, wetland and agriculture ecosystems, and 50 species were identified based on morphological characteristics and sequence analysis of genetic markers. Trichoderma harzianum showed the largest proportion of isolates and the most extensive distribution. Hypocrea semiorbis, T. epimyces, T. konilangbra, T. piluliferum, T. pleurotum, T. pubescens, T. strictipilis, T. hunua, T. oblongisporum and an unidentified species, Trichoderma sp. MA 3642, were first reported in China. Most Trichoderma species were distributed in Jilin and Heilongjiang Provinces in northeast China and the fewest were distributed in Qinghai Province. Based on the division of ecological and geographic factors, forestry ecosystems and low-altitude regions have the greatest species biodiversity of Trichoderma.

High‑speed growth of high‑quality polycrystalline diamond films by MPCVD

Kai Chen,Tao Tao,Wenxiao Hu,Yucong Ye,Kaiwen Zheng,Jiandong Ye,Ting Zhi,Xiwei Wang,Bin Liu,Rong Zhang 한국탄소학회 2023 Carbon Letters Vol.33 No.7

High-quality diamond films have attracted extensive attentions due to their excellent optical and electrical properties. However, several issues, such as random orientation, stress accumulation, and slow growth rate, severely limit its applications. In this paper, high-quality polycrystalline diamond films with highly ordered (100) orientation were prepared by microwave plasma chemical vapor deposition. The effects of growth parameters on the microstructure, quality and residual stress of diamond films were investigated. Experimental results indicate that relatively high temperature at low methane concentration will promote the formation of (100) oriented grains with a low compressive stress. Optimized growth parameters, a methane concentration of 2% along with a pressure of 250 Torr and temperature at 1050 ℃, were used to acquire high growth rate of 7.9 μm/h and narrow full width at half maximum of Raman peak of 5.5 cm? 1 revealing a high crystal quality. It demonstrates a promising method for rapid growth of high-quality polycrystalline diamond films with (100) orientation, which is vital for improving the diamond related applications at low cost.

All Carbon Dual Ion Batteries

Hu, Zhe,Liu, Qiannan,Zhang, Kai,Zhou, Limin,Li, Lin,Chen, Mingzhe,Tao, Zhanliang,Kang, Yong-Mook,Mai, Liqiang,Chou, Shu-Lei,Chen, Jun,Dou, Shi-Xue American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.42

<P>Dual ion batteries based on Na<SUP>+</SUP> and PF<SUB>6</SUB><SUP>-</SUP> received considerable attention due to their high operating voltage and the abundant Na resources. Here, cheap and easily obtained graphite that served as a cathode material for dual ion battery delivered a very high average discharge platform (4.52 V vs Na<SUP>+</SUP>/Na) by using sodium hexafluorophosphate in propylene carbonate as electrolyte. Moreover, the all-carbon dual ion batteries with graphite as cathode and hard carbon as anode exhibited an ultrahigh discharge voltage of 4.3 V, and a reversible capacity of 62 mAh·g<SUP>-1</SUP> at 40 mA·g<SUP>-1</SUP>. Phase changes have been investigated in detail through in situ X-ray diffraction and in situ Raman characterizations. The stable structure provides long life cycling performance, and the pseudocapacitance behavior also demonstrates its benefits to the rate capability. Thus, dual ion batteries based on sodium chemistry are very promising to find their applications in future.</P> [FIG OMISSION]</BR>

A study on convergence and complexity of reproducing kernel collocation method

Hu, Hsin-Yun,Lai, Chiu-Kai,Chen, Jiun-Shyan Techno-Press 2009 Interaction and multiscale mechanics Vol.2 No.3

In this work, we discuss a reproducing kernel collocation method (RKCM) for solving $2^{nd}$ order PDE based on strong formulation, where the reproducing kernel shape functions with compact support are used as approximation functions. The method based on strong form collocation avoids the domain integration, and leads to well-conditioned discrete system of equations. We investigate the convergence and the computational complexity for this proposed method. An important result obtained from the analysis is that the degree of basis in the reproducing kernel approximation has to be greater than one for the method to converge. Some numerical experiments are provided to validate the error analysis. The complexity of RKCM is also analyzed, and the complexity comparison with the weak formulation using reproducing kernel approximation is presented.

Joint Simulation of Trolley Vehicle-Frame Structure Coupled Vibration Using ADAMS and ANSYS for Container Crane Simulated Training System

Kai-liang Lu,You-fang Huang,Wei Yan,Jun Hu,Chao Chen,Dao-fang Chang 보안공학연구지원센터 2013 International Journal of Hybrid Information Techno Vol.6 No.5