Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고, 그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다 1. B. abortus 분리주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행 한 바 공시한 참조균주와 분리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.6%~l00% 및99.5%~99.9%의 매우 높은 상품성을 나타내었고, B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상품성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바, 분리균주들은 B. abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%~l00%의 매우 높은 상품성을 보였으며, B. abortus 45/20 (biovar 1)과는 96.4%~97.2%의 상품성을 보였다. 3. 본 시험에 공시된 브루셀라균주의 omp2b 유전자서열과 여러 동물에서 분리된 브루셀라 분리주의자료를 바탕으로 phylogenetic tree를 작성 한 바, 국내 분리주는 B. abortus 2308 (biovar 1), B. abortus B3196 (blovar 5), B. abortus 45/20 (biovar 1), B. suis 1330 (biovar 1), B. melitensis 16M (biovar 1), B. canis NCTC 01854, B. neotomae 5k33과 97.3%이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며, omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OW) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa OMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%-100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기 위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고,그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다. 1. B. abortus 분리 주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행한 바 공시한 참조균주와 분 리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다. 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리 균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B abortus B3196 (biovar 5)과는 99.6%∼100% 및 99.5%∼99.9%의 매우 높은 상동성을 나타내었고,B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상동성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바,분리균주들은 B.abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%∼100%의 매우 높은 상동성을 보였으며,B. abortus 45/20 (biovar 1)과는 96.4%∼ 97.2%의 상동성을 보였다. 3. 본 시험에 공사된 브루셈라균주의 omp2b 유전자 서열과 여러 동물에서 분리된 브루셀라 분리주의 자료를 바탕으로 phylogenetic tree를 작성 한 바,국내 분리주는 B. abortus 2308 (biovar 1),B. abortus B3196 (biovar 5),B. abortus 45/20 (biovar 1),B. suis 1330 (biovar 1),B. melitensis 16M (biovar 1),B. canis NCTC 01854,B. neotomae 5K33과 97.3% 이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며,omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OMP) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa aMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%∼100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

A Novel Ultrasonic Motor Using Orthogonal Bimorphs

Jung-Moo Seo,Jin Hur,Ha-Gyung Sung IEEE 2007 IEEE transactions on magnetics Vol.43 No.4

<P>The main purpose of this paper is to describe a novel ultrasonic motor which is suitable for small information devices. With a bending mode of a bimorph structure having relatively large displacement, the input voltage and frequency could be reduced. The overall shape of the motor is very simple, so it could be manufactured and miniaturized with ease. From the simulated results, such as mode and harmonic analysis, resonance mode, operating frequency, and displacement are determined. Finally, the experimental results are compared with the simulated</P>

국내 유통 소금 중의 포타슘시아니이드와 페로시아나이드염의 분석에 관한 연구

허숙진,소유섭,최장덕,이화정,최선희,성준현,임무혁,김광수,최용훈,오해성,최정희,최정실,허옥순,이진하,신동우,신현수,문병우 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 연구는 국내 유통 소금중의 안전성을 규명하기 위하여 천일염 54건, 재제염 보건, 가공염 33건 등 총 143건에 대하여 숲행하였다. HPLC, ICP, Mercury Analyzer 등을 이용하여 KCN, 페로시아나이드 염, 납 등 6개 성분의 항량을 측정하였으며 그 결과는 다음과 같다. [rdn-max(mean) ; m곱3 ; f(CN : ND-ND(ND), 페로시아나이드염 : ND-ND(ND), Pb . ND-1.30 (0.331), Cd :ND-0.23(0.070), Hg ND-0.35(0,035), As :ND-1.52fo.062). 또한, 신속하고도 효율적이 면서 기존의 환경부 시안분석법의 단점을 보완할 수 있는 KCN과 페로시아나이드염의 분석 조건을 계시하였으며, 천일염, 재제염, 가공염 뿐만아니라 소금이 첨가된 식품까지도 적용하기 위한 기초 자료로 활용이 가능할 것으로 사료된다. This study was conducted to confirm the safety in a total of 143 samples of salts. The contents of potassium cyanide(KCN), potassium ferrocyanide(K_(4)Fe(CN)_(6)), lead(Pb), cadmium(Cd), mercury(Hg), and arsenic(As) were determined using a high performance liquid chromatograph(HPLC), inductively coupled plasma spectrometer(ICP), and mercury analyzer. The values of cyanide complexes and metal elements in salts were as follow [min-max(mean);mg/kg] ; KCN : ND-ND(ND), Ferrocyanide salt : ND-ND(ND), Pb : ND-1.30(0.331), Cd: ND-0.23(0.070), Hg : ND-0.35(0.035), As : ND-1.52(0.062). In this study, we established efficient

직립마모 저감을 위한 원호형 차륜답면형상안 마모특성 분석

허현무(Hur Hyun-Moo),권성태(Kwon Sung-Tae),서정원(Seo Jung-Won),이찬우(Lee Chan-Woo) 한국철도학회 2004 한국철도학회 학술발표대회논문집 Vol.- No.-

We proposed new arc-type wheel profiles to improve severe flange wear of conventional wheel profile coned 1:40. We designed many candidate wheel profiles and analyzed the geometric characteristics, dynamic performance and stress at contact points to draw out the final profiles. From the analysis result, we chose two cases of profiles and applied to wheels of test train. Tests carried out in service line to analyze the dynamic performance and verify the wear reduction for two cases of profiles. Test results shows the equal level of dynamic performance and the improvement of wheel flange wear compared with the conventional wheel profile.

고속철도 차륜의 마모유형에 관한 연구

허현무(Hur Hyun-Moo),서정원(Seo Jung-won),서승일(Seo Sung-Il) 한국철도학회 2005 한국철도학회 학술발표대회논문집 Vol.- No.-

Korea High Speed Railway of which wheels have been excessively worn during the trial operation prior to opening was additionally applied with solid lubricant besides the existing oil lubricant in order to overcome such excessive wear. Therefore, it was shown that wear status has been improved in the test by which railway system including vehicles and tracks was stabilized during a year"s commercial operation, comparing to that with excessive wear in the trial operation prior to opening to the public. In this connection, wheel"s wear per mileage was analyzed, based on vehicles in service in order to make out the wheel"s wear at present, from which the useful data relating to wheel maintenance could be obtained.

Fatigue Design Evaluation of Railway Bogie with Full-Scale Fatigue Test

Seo, Jung-Won,Hur, Hyun-Moo,Jun, Hyun-Kyu,Kwon, Seok-Jin,Lee, Dong-Hyeong Hindawi Limited 2017 Advances in materials science and engineering Vol.2017 No.-

<P>The bogie frame of a railway is an important structural member for the support of vehicle loading. In general, more than 25 years’ durability is necessary. Much study has been carried out in experimental and theoretical domains on the prediction of the structural integrity of the bogie frame. The objective of this paper is to estimate the structural integrity of the bogie frame of an electric railcar. Strength analysis has been performed by finite element analysis. From this analysis, stress concentration areas were investigated. To evaluate the loading conditions, dynamic stress was measured by strain gauge. It has been found that the stress and strain due to the applied loads were multiaxial conditions according to the location of the strain gauge. Fatigue strength evaluations of the bogie frame were performed to investigate the effect of a multiaxial load through the employment of a critical plane approach.</P>

외팔보 직교형 초음파 모터 개발

서정무(Jung-Moo Seo),허진(Jin Hur),성하경(Ha-Gyung Sung) 대한전기학회 2006 대한전기학회 학술대회 논문집 Vol.2006 No.7

The main purpose of this paper is to describe a novel ultrasonic motor which is suitable for small information devices. With a bending mode of a bimorph structure having relatively large displacement, the input voltage and frequency could be reduced. The overall shape of the motor is very simple, so it could be manufactured and miniaturized with ease. From the simulated results, such as mode and harmonic analysis, resonance mode, operating frequency, and displacement are determined. The experimental results are compared with the simulated one finally.

거푸집 패널이 부착된 2방향 중공슬래브의 뚫림 전단 안전성 평가

허무원 ( Moo-won Hur ),우형식 ( Hyung-sik Woo ),박정민 ( Jung-min Park ),강현욱 ( Hyun-wook Kang ),박태원 ( Tae-won Park ) 한국구조물진단유지관리공학회 2021 한국구조물진단유지관리공학회 논문집 Vol.25 No.5

VPS는 중공재의 형상을 최적화 하고 중공재의 부상 및 작업하에 의한 이탈을 방지하는 기능을 가진 기존 중공슬래브공법이다. 본 연구에서는 기존연구에서 제안한 거푸집 패널이 부착된 중공슬래브(VPS)를 활용하여 플랫 플레이트의 뚫림전단 안전성을 검토하였다. 실험 결과, 가력점으로 부터 기둥 폭의 2.0배를 넘어 중공재를 배치한 VSPS 실험체는 기준 실험체에 대비 내력이 9.4%감소하였다. KBC2016에서 제시한 설계 값의 약 1.57배 이상의 강도 값을 나타내었다. 중공재를 배치한 VSPS 실험체가 전단파괴가 발생하기 전까지 기준 실험체 대비 강성의 변화는 없는 것으로 나타났다. 이는 휨 철근을 충분하게 배근하여 본 실험이 전단에 의해 파괴되고 있음을 알 수 있다. VPS(Void Plywood Slab System, VPS) has optimized the shape of the hollow material. In addition, it has a function to prevent the floating of the hollow material and the separation due to the working load. In this study, the punching shear capacity of flat plate was performed using Void Plywood Slab System with form work panel proposed in the previous study. As a result of the test, the strength of the VSPS specimen in which the hollow material was placed beyond 2.0 times the column width from the loading point was reduced by 9.4% compared to the reference specimen. However, the strength value was about 1.57 times higher than the design value suggested by KBC 2016. It was found that there was no change in stiffness compared to the reference specimen until shear failure occurred in the VSPS specimen in which the hollow material was placed. It can be seen that this experiment is being destroyed by shear as the flexural reinforcing bars are sufficiently reinforced.

가토에서 골반경수술과 개복수술 후 유착 및 조직학적 반응에 대한 비교 연구

서정호,박형무,배도환,허민 중앙대학교 의과대학 의과학연구소 1995 中央醫大誌 Vol.20 No.4

Development of adhesions following pelvic surgery is a leading failure of reconstructive surgery. The aims of this study were to compare the occurrence of adhesions after a standard uterine injury inflicted by laparoscopy versus laparotomy, to compare the efficacy of 32% dextran 70 used for adhesion prophylaxis and to compare the tissue reactions to the catgut, polypropylene, polyglactin and polproydioxanone suture materials. Forty rabbits were randomly assigned to four groups, of which group 1 and 2 received operations via laparoscopy and group 3 and group 4 received via laparotomy. During operations, group 1 and group 3 were irrigated and administered with 10㎖ of 32% dextran 70. The uterine horns were incised and anastomosed with four suture materials respectively. After six weeks, thirty eight rabbits were assigned to laparotomy to assess the extent of intraperitoneal adhesions. The intraperitoneal adhesions were graded according to their severity. Tissue block were obtained from the suture sites for histologic assessment. Adhesions were significantly less in laparoscopy-treated group than in laparotomy treated group but there are no differences in the inflammatory reactions to suture material, operative manipulations or 32% dextran 70. Moderate fibrosis persisted around the catgut and polypropylene suture, but not at all around the polydioxanone and polyglactin sutures. The routine use of intraperitioneal 32% dextran 70 has been suggested as an adjuvant to prevent postoperative adhesions, however it's used die not reduce the formation of adhesions in this study.