Protective Effects of Portulaca oleracea L. Extract against Matrix Metalloproteinase Production and Reactive Oxygen Species Generation Induced by Ultraviolet B Radiation in Human Keratinocytes

Jung Hwan Oh(오정환),Fatih Karadeniz(파티 카라데니즈),Jung Im Lee(이정임),So Young Park(박소영),Youngwan Seo(서영완),Chang-Suk Kong(공창숙) 한국생명과학회 2018 생명과학회지 Vol.28 No.8

쇠비름(Portulaca oleracea.L)은 쇠비름과에 속하는 한해살이풀로서 리놀렌산과 같은 불포화지방산, 페놀성 화합물, 플라보노이드, 비타민 C, 미네랄 함량이 높은 것으로 보고되어 있다. 본 연구에서는 쇠비름 추출물을 이용하여 UVB를 조사한 인간각질형성세포에서 광노화 억제능을 확인하였다. Matrix metalloproteinases는 세포의 기질을 분해하는 효소로 MMP-1는 collagenase, MMP-2와 MMP-9는 gelatinases로 피부 진피층을 구성하는 type Ⅰ collagen을 분해시키는데 영향을 미친다. UVB를 조사한 인간각질형성세포에서 쇠비름 추출물을 처리했을 때 MMP-1, -2, -9의 발현이 감소하였으며, type Ⅰ procollagen의 발현은 증가하는 것으로 나타났다. 또한 쇠비름 추출물을 처리한 군에서 UV에 의한 ROS 생성이 감소하였는데 이는 Nrf-2의 활성화를 통한 항산화 인자 SOD-1과 OH-1의 발현 증가로 인해 세포내 ROS 생성이 감소한 것으로 사료된다. 따라서 본 연구 결과를 통해 쇠비름 추출물이 UVB를 조사한 인간각질형성세포에서 MMP 인자 및 항산화 인자의 발현 조절을 통해 광노화로부터의 세포 보호능을 가지는 것을 확인하였으며 나아가 화장품 소재로서의 개발 가능성을 확인하였다. Portulaca oleracea L. is an edible plant widely consumed in daily diet throughout Europe, Asia and America. In this study, protective effects of P. oleracea L. extracts against oxidative stress and matrix metalloproteinase (MMP) activity induced by ultraviolet B (UVB) radiation were investigated using HaCaT immortal human keratinocytes. In this context, the mRNA and protein productions of MMPs (MMP-1, -2, and -9) and type I procollagen, which are major markers of photoaging induced by UVB radiation in HaCaT keratinocytes, were evaluated. Furthermore, UVB-induced reactive oxygen species (ROS) generation and mRNA and protein expression levels of superoxide dismutase-1 (SOD-1), oxygenase-1 (OH-1), and nuclear factor-erythroid 2-related factor-2 (Nrf-2), all of which are associated with the antioxidant balance, were investigated. As shown by the results, UVB radiation induced ROS formation and led to increased production of MMPs and decreased collagen production in human keratinocytes, which resulted in skin photoaging or photodamage. The treatment with P. oleracea L. extracts downregulated MMP (MMP-1, -2, and -9) production and upregulated type I procollagen expression in UVB-induced HaCaT cells. Furthermore, treatment with the extracts decreased UVB-induced ROS generation and increased the expression of antioxidant enzymes, such as SOD-1 and OH-1, through the Nrf-2 pathway. Taken together, these results suggest that P. oleracea L. extracts could be a potential cosmeceutical agent for the prevention of skin photoaging or photodamage.

비만증 환자에서 한약제에 의한 체중 감소효과

Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.

컨벤션센터의 食飮科서비스 運營方案

오정환 한국문화관광학회 1999 문화관광연구 Vol.1 No.1

The Convention Industry came to have footlights as a high supplement industry owing to the expansion of international cooperation and mutual interchange. The demands of the convention Industry is upsurging and the scale of it is enlarged. Now many countries focus on the convention as one of the chief industries. Because of the tendency of globalization, Convention(MICE : Meetings, Incentive Travels, Conventions, Expositions) Industry has high priority among many Industries as a main income source by entertaining foreign tourists. The purpose of this article is to establish a theory for Food & Beverage Facilities of Convention Center, and to conceptualize the efficient operation of convention F&B facilities. Through the critical overview of the related literature the overall concept, kind of considerations of International convention industry studies were delineated. This study is composed of five parts; Ⅰ. Introduction to the purpose and method of this study Ⅱ. The change environment of convention industry in Korea Ⅲ. Framework of the theory Ⅳ. The efficient operation for F&B Facilities of Convention Center Ⅴ. Conclusions The author hopes advancements of this study, and utilization of study results to the empirical study.

矮性사과나무의 粗皮症狀 發現要因에 關한 硏究 : 粗皮症狀 發現樹의 無機養分 및 炭水化物 含量 Mineral and Starch Contents of Dwarf Apple Trees with Rough Barks

吳中煥 안성산업대학교 1992 論文集 Vol.24 No.-

본 연구는 粗皮症狀의 原因을 究明함에 있어스는 樹體內 無機成分과 炭水化勿이 조피증상과 어떤 關聯이 있는가를 보기 위하여 粗皮症狀 發現樹의 各部位別 無機成分과 炭水化勿의 含量을 健全樹와 比較하여 調査하였던 바 그 結果를 要約하면 다음과 같다. 1. 葉, 新梢, 主幹樹皮의 無機成分 含量에 있어 Mg, Ca, Mn, Fe, Zn, B, C 成分들이 모두 粗皮發現樹와 健全樹間에 뚜fut한 差異를 볼 수 없으며 健全 果樹園에서의 후지와 쓰가루 品種간에도 차이를 認定할 수 없었다. 2. 뿌리의 無機成分 含量은 대부분 健全樹와 粗皮發現樹간에 差異가 없었으나 Ca 含量은 被害樹가 健全樹보다 낮은 傾向이었고 Fe, Zn의 含量은 粗皮發現樹가 有意하게 낮았다. 3. 主幹樹皮를 內樹皮와 外樹皮로 區分하여 無機成分 含量을 分析해 본 結果, Ca를 除外한 Mg, Mn, Fe, Zn, B는 外樹皮의 含量이 더 높았으며 모든 無機成分이 粗皮 發現樹의 內皮樹에서 含量이 낮았다. 特히 후지 品種의 內樹皮에서 Zn 含量은 粗皮 發現樹에서 高度로 有意하게 낮았다. 4. 粗皮症狀 發現樹에서 뿌리의 炭水化勿 含量이 正常樹에 비하여 全生育期間에 걸쳐서 全般的으로 낮았다. 그러나 品種간 比較에서는 후지 品種이 쓰기루 品種보다 높았다. This study was conducted to investigate the effect of mineral and starch contents of the trees on the rough bark disorder in the dwarf apple trees. The sympton of the disorder may be similar to that of apple measles induced by manganese toxicity or boron deficiency. But this symptom develops on the stem bark, whereas Mn toxicity and B deficiency occur from one and two year-old twigs. The obtained results were summarized as follows: 1. Mineral contents such as Mg, Ca, Mn, Fe, B, Zn and Cu in the leaf, shoot and trunk bark showed little difference between the rough-bark and a normal tree. 2. Ca content in root of disordered 'Fuji' tree was significantly lower than that of normal trees. 3. Contents of Mg, Mn, Fe, b and Zn with the exception of Ca in the outer bark of trunk were higher than those in the inner bakr in a normal tree. Those minerals with the exception of Mn were significantly lower in the inner bark of the disordered tree, particularly Zn content was highly significantly lower as compared with that in the inner bark of a normal tree. 4. Starch and glucose contents in the stem bark and roots of the disordered tree were lower than in the normal tree throughout the year. However, 'Fuji' showed higher starch concentration than 'Tsugaru'.

압력용기의 설계기준 및 손상 평가

오환섭,정효진,박상필,손두익 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

The damage of the pressure courage by degradation can become the reason of unexpected break down or failure accident and it is very important because safety accident, the production loss, environmental pollution, social problems are occur. Consequently The result to investigat of failure accident for domestic pressure vessel, the factor of degradation is SCC, Sorrosion, Cavity, Crack

배나무잎 검은점병의 발생원인 조사

오중환 안성산업대학교 1996 論文集 Vol.28 No.-

Symptom development of pear abnormal leaf spot was highest at the day/night temperature regimes of 23/18℃, and reduced remarkably at the higher and lower temperature regimes as 28/23℃ and 18/13℃. When the pear leaves were bagged or wrapped with various covering materials, little or no symptoms were developed. This was considered to be due to the masking effect of the symptom development induced by high temperatures as up to 46.8℃ after leaf covering. Chemical properties of soil samples from diseased and healthy trees did not show a consisent trend except phosphate whose content was higher in the soil from diseased trees. Simptom development was delayed when moisture levels in soil was over on below the optimum conditron, although the final disease severity did not differ greatly.

친환경농업 농가 컨설팅에 대한 반응 조사

오환중,차광홍,정우진,김길용,박노동 全南大學校 農業科學技術硏究所 2011 農業科學技術硏究 Vol.46 No.-

The interactions between Environment-Friendly Agriculture Research Center (EFARC) and farmers in Jeonnam Province area who have practiced environment-friendly agriculture (EFA) under guidances of EFARC were investigated through enquete. The enquete was conducted during July - September, 2010. One hundred and eighty nine farmers responded all inquiries among 200 farmers. The main results are summarized as follows: 1. Rice was the predominant crop grown by farmers (49%), and the next major was hot pepper (12%). Most farmers (95.3%) have practiced environment-friendly agriculture at the levels of no-pesticide (55.6%), lower pesticide (32.8%), and organic (6.9%). 2. Major motivations of practicing EFA are for the production of safe foodstuffs (52.6%) and for an increase of income (40.1%). 3. Around 3 quarters of the environment-friendly agriculture products are shipped through Nonghyup, the cooperative marketing. The price margin between ordinary agriculture products and EFA products was of 5~10% for 63.5% farmers, of 10~20% for 14.3% ones, and of 20~30% for 14.8% ones. 4. Bottlenecks for practicing EFA are in the order of the unstability of shipping price (34.9%), cultivation techniques (20.1%), excess labor input (19.6%), and control of disease and insect pest (18%). 5. It turned out that more than 90% farmers who have practiced EFA under guidance of EFARC recognized the consulting activities of EFARC as to be helpful for them. 6. Establishment of national organizations which are specialized in consultation for EFApracticing farmers has been suggested.

Stabilized 위성안테나의 PCU 제어알고리즘의 구현

오정환,김종철 慶南專門大學 1998 論文集 Vol.26 No.1

배나무잎 이상반점 증상의 접목전염에 관한 연구

오중환 안성산업대학교 1995 論文集 Vol.27 No.-

배나무잎 이상반점 증상의 접목전염 여부를 온실과 포장에서 여러가지 접목방법으로 시험 하였다. 이병가지와 건전가지를 2월에 채취하여 건전한 실생대목에 접목한 결과 이병가지를 접목한 가지에서만 발병되었다. 또한 이병가지와 건전가지를 각각 첫 번째 접수와 두번째 접수로 하여 건전한 실생대목에 접목했을때도 예외 없이 두번째 접수에서의 발병이 확인되었다. 이병주와 건전주를 기부에 설접(舌接)하여도 두주 모두 발병하였다. 지표식물 HN-39를 두번째 접수로 이용하고 첫번째 접수를 이병가지로하여 배나무 실생대목에 접목하면 HN-39에서 심하게 발병하였다. 농업과학기술원 과수원에서 건전주에 이병가지를 고접하면 고접한 당년에는 접수에서만 발병되었고 다음 해에는 고접한 주지에, 3년차에는 배나무 전체가 심하게 발병되었다. 이상의 결과로 볼 때 배나무 이상반점증상은 병원이 접목전염하는 것으로 생각된다. Nature of graft transmissibility of pear necrotic leaf spot disease was examined by various grafting methods in the greenhouse and field. When the diseased and symptomless twigs were collected in winter and grafted in the next spring to the seed-originated healthy root stock, the abnormal leaf spot was developed only in the case of the diseased twigs. Double grafting on a seed-originated healthy root stock, where the diseased and the symptomless twigs were used as 1st and 2nd scion, respectively, developed necrotic leaf spot lesions without exception on the 2nd scion. Tongue-graft with the diseased and the symptomless trees also incited abnormal leaf spots on the both trees. Necrotic leaf spots were also developed on HN-39, an indicator pear tree, used as a 2nd scion in a double graft test, where the diseased twig and a seed-originated healthy tree were used ad the 1st scion and the root stock, respectively. when the diseased twig was top-grafted to the healthy root stock, lesion developtment of necrotic spot was limited to the grafted twig itself in the 1st yea, but expanded to the main branches in the 2nd year, and spread over the whole tree in the 3rd year. This result indicates that the causal agent of necrotic leaf spot disease is transmitted by graft.

상업스포츠센터 종사자의 직무만족도에 관한 연구

오종환 목포대학교 스포츠 산업연구소 2004 스포츠 산업연구소 논문집 Vol.3 No.-

This study on the commercial sports managers' their official satisfactions. For the purpose of that, we shall study the relations of the personal characters and their identities and the relations of their organization satisfactions and their identities targeting 135 managers engaged in sports centers so that we provide the managers with basic datum for their management of a change of occupation. Moreover they can increase the satisfaction of the customers using the sports centers, and the managers can also become higher their official satisfaction and their engagement identities. After verifying one-way ANOVA and post-test using SPSS 10.0version, we can reach a conclusion below listed. A. The relations between personal characters and their organization identities 1. There is no difference between the level of the manager's organization identity and their sex. 2. There is no differences between the level of the manager's organization identity and their age. 3. There is some differences between the level of the manager's organization identity and their salary. 4. There is some differences between the level of the manager's organization identity and their duties. 5. There is no difference between the level of the manager's organization identity and scholarship. 6. There is some differences between the level of the manager's organization identity and their period of service in the position B. The relations between their official satisfaction and their organization satisfaction. A constant is 1.376 and a value conforming to the official satisfaction is 0.725. So we can come to a conclusion because each presumed value is smaller than significance level 0.05