무세포혈관과 자가 골수줄기세포를 이용한 조직공학적 인공혈관 제조

김동익,김병수,조승우,임정은,어현선,이민재,김종성,장인성 대한혈관외과학회 2004 Vascular Specialist International Vol.20 No.1

스프레이 菊花의 왕겨경 循環式 養液栽培에서 養液의 濃度 및 供給回數의 영향

黃仁澤,金光秀,金月洙,金道翊,金正根 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

본 실험은 스프레이 국화 왕겨 배지를 이용한 순환식 양액재배에 있어서 양액의 적정농도와 공급횟수를 구명하고자 실시한 결과 양액 농도 처리에서는 원시표준액의 1.6배 처리구가 초장, 화경장, 경경, 화경수, 엽장, 엽폭, 절화중에서 가장 양호하였고, 1일 공급횟수는 표준농도(1.0배)는 12회가 좋았고 1.3배와1.6배 처리구에서는 6회공급구에서 절화품질이 좋았다. 양액농도별 개화기는 표준농도에 비해 1.3배는 2일, 1.6배 처리구는 3일정도 지연되었다. 양액 농도별 T-N함량은 1.3배 농도에서 많았고 양이온치환용량은 농도가 높은 1.6배 농도에서 높았으며 양액 농도별 엽록소 함량은 1.0배 농도에서 가장 높았다. This experiment was conducted to determine the optimal ionic strength of nutrient solution and fertigation frequency affecting growth and development of spray chrysanthemum (Dendranthema grandiflorum) in recycling system using rice hull. Growth parameters in terms of plant height, flowers diameter, stem diameter, number of flowers, leaf length and width and flower weight were shown better in thd 1.6 ionic strength of nutrient solution than those of 1.0 ionic strength of nutrient solution. Fertigation frequency was good at 12 times during day and night in the standard solution (1.0 ionic strength) while 6 times per day and night were good in both 1.3 and 1.6 strength of nutrient solution. Flowering time was postponed up to 2 day in the treatment of 1.3 ionic strength of nutrient solution, and 3 day in 1.6 ionic strength of nutrient solution. The content of total nitrogen was higher in the 1.3 ionic strength of nutrient solution. Cation exchange capacity was high in the 1.6 ionic strength of nutrient solution and chlorophyll content by the concentrations of nutrient solution was highest in the standard nutrient solution.

가족인형놀이가 정신지체 아동의 자발적 발화에 미치는 효과 : 사례연구 Case Study

김윤선,김수정,이경희,한현민 대진대학교 교육대학원 교육연구소 2002 교육연구 Vol.4 No.1

The experiment was undertaken to determine the effect of family puppet play on spontaneous utterance of a five-year-old child with mental retardation. The intervention was three major activites, that is the meeting with father, the playing with elder brother, and the eating time with mother. The intervention continued over 15 weeks, and lasted 26 sessions (20 minutes a session). The data gathered were response to imitation demand, spontaneous imitation, spontaneous utterance, and emotion expressive utterance. The results revealed that in initial sessions response to imitation demand and spontaneous imitation were dominated, but spontaneous utterance and emotion expressive utterance were rapidly increased after 10th and 12th session, respectively.

정착마을 나음성자에 대한 임상적 관찰 : 일제검진 성적을 중심으로

金基洪,鄭守和,엠마프라이징거 大韓癩學會 1984 癩學 會誌 Vol.17 No.1

치경부 5급 와동 수복물의 표면 거칠기와 미세누출에 관한 연구 : 표면 전색의 효과

김민정,이미정,유미경,박수정,이광원 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.1

The purpose of this study was to compare the effect of surface sealing materials on microleakage and surface roughness in Class V composite restorations. Twenty five standardized Class V cavity preparations were made on the facial surface of human premolars and were randomly assigned to 5 groups. The teeth were restored with Z-250 after applying Single Bond. Following 7 days storage in distilled water at 37℃, the restorations were sealed as following systems : No sealing ; Single Bond Adhesive ; Biscover ; Fortify ; Optiguard. Then, toothbrush abrasion test was conducted using a wear testing machine. Surface roughness was measured by means of profilometer before and after toothbrushing and the results were statistically analysed by using a paired t-test and ANOVA. The bonded interfaces and the changes of surface roughness were examined by SEM. For microleakage test, specimens were stained in a 2% methylene blue solution, then longitudinally sectioned and analyzed for leakage at occlusal and cervical interfaces using stereomicroscope. The results were statistically analysed by using a Kruskal-Wallis and Mann-Whitney U test. Surface roughness was increasing in all groups after toothbrushing, but no statistically significant differences. In SEM observation, surface sealant was partially retained and partially detached in bonded interfaces. Especially, microgap was identified in cervical margins. In microleakage test, there was better seal in the enamel region and a significant difference between groups at occlusal margin. Control group and Single Bond group had significantly better marginal seal at enamel margin than cervical margin. 수복물의 변연 미세 누출과 마모 개선을 위해 표면 전색제가 개발되었으며 이의 효과에 대해 알아보기 위해, 규격화된 치경부 5급 와동을 25개의 소구치의 협면에 형성하고 Single Bond와 Z-250으로 수복한 뒤, 37℃의 증류수에 7일간 보관한 후 무작위로 5군으로 나누고 다음과 같은 재료로 제조사의 지시에 따라 표면 처리하였다 : No sealing ; Single Bond Adhesive ; Biscover ; Fortify ; Optiguard. 그 후 잇솔질 마모시험을 시행하였다. 표면 조도는 잇솔질 전후에 profilometer로 측정되었고 paired t-test와 ANOVA로 비교하였다. 접착면과 표면변화는 전자현미경으로 관찰하였다. 미세누출 평가를 위해 시편은 2% methylene blue 용액으로 염색한 뒤, 협설로 잘라 제작하였다. 입체현미경을 이용하여 교합면쪽과 치경부쪽 계면에서 미세누출을 관찰하고 Kruskal-Wallis와 Mann-Whitney U test를 이용하여 그 결과를 비교하였다. 표면 조도는 잇솔질 후 수치가 증가하였으나 통계학적으로 유의한 차이를 나타내지는 않았다. 전자 현미경 관찰 결과, 표면 전색제가 접착면에 부분적으로 남아있기도 하였으나 많은 부분에서 떨어져 나간 상태였다. 특히, 미세 틈새가 치경부쪽 변연에서 나타났다. 미세누출 실험에서는 법랑질에서 더 봉쇄효과가 좋았으며 교합면쪽 변연에서 군간에 유의할만한 차이를 나타내었다. 조절군과 Single Bond를 도포한 군에서는 치경부쪽 변연보다 법랑질쪽 변연에서 유의하게 좋은 변연 봉쇄 효과를 나타내었다.

규폐결핵 3례의 임상 및 병리학적 소견의 비교 검토

김상호,이수영,윤임중,이승한,조규상,김영제 카톨릭대학산업의학센타 산업의학연구소 1972 韓國의 産業醫學 Vol.11 No.3

Among the pneumoconiosis, the silicosis is an occupational lung disease, ordinally due to prolonged inhalation of air containing free silicon dioxide. We had chance to perform autopsy of three cases of silicotuberculosis. Both lungs with hard and contracted appearance, were showed massive fibrous adhesion to pleura and also revealed gritty appearance on cut sectioning, and the cut surfaces were shown dark greenish to black pigmentation. Characteristically, many dense black or grey colored silicotic nodules measuring upto 1-4 mm in diameter, with anthracotic pigments distributed throughout both lung parenchyma especially along the peribronchial regions in all three cases, commonly. Microscopically, the nodules were noted as the mass of concentrically laminated dense fibrotic tissue. In addition to those findings, the pulmonary parenchymas were replaced by wide areas of fibrosis and also accompanied with exudative and productive form of tuberculosis (common in all three cases), bronchopneumonia (except case-1) and also compensating emphysema, atelectatic lesions. At the peripheral areas of the silicotic nodules, focal accumulation of the plasma cells, lymphocytes and many foci of endarteritis were shown in three cases, commonly. The silica was confirmed by polarizing microscopy as birefringent, white colored crystals.

嶺湖南地方 瘠薄林地의 오리나무와 아까시나무의 適地에 關한 硏究

金昌浩,鄭印九,吳正壽 동국대학교 농림과학연구소 1969 農林科學 論文集 Vol.3 No.-

The objectives of these studies were to investigate how every kinds of Black Locust and Alder influence on the growth of woods in the Yongnam and Honam district. The result of this study appeared as follows: 1. At the point of soil depth. the deeper the soil depth is, the better for Black Locust and Alder grows well regardless of it. 2. Black Locust shows good growth in loose soil but even in consistant soil Alder was excellently brought up. 3. Black Locust grows well in clay loam while Alder is appropriate to grow in sandy soil. 4. Black Locust requires an adequate soil depth so it grows well in gentle slope area but Alder grows well even in steep slope area which unproductive land enables to grow Alder satisfactorilly. 5. The constitution of cover vegetation could, to some extent, be attained in the superior forest land of Black Locust but it was impossible to make cover vegetatioe in that of Alder : It can therefore, be easily attained the open area between trees of Alder pure forest. 6. Black Locust and Alder are soil improving trees and so the content of effectivn phosphorus had a great effect on tree growth. 7. Black Loust forest land had more organic substance than Alder and this fact was closely connected with tree growth. 8. C. E. C is deeply concerned with the growth of black locust but less concernment related with Alder. 9. The suitable land for Black Locust. 1) A deep soil depth. 2) On less consistancy. 3) Contained much of P_2O_5 in soil. 4) Much of organic substance. 5) On a clay loam. 6) Forest land with greater C. E. C. 10. The suitable land for Alder : 1) Contained much of P_2O_5 in soil. 2) Much of organic substance. 3) With moderate consistancy and effective soil depth 4) on sandy soil. 11. It is assumed that Black Locust requires more soil conditions than Alder. 12. Haenam district of Chulanam-Do where severly damaged by disease was excluded from this survey study.

Coacervation-상분리법으로 만든 Ethyl Cellulose 마이크로캅셀의 Glibenclamide 방출특성 및 수용성 첨가제의 영향

김병조,장정수,이문주,김상헌,최영욱 중앙대학교 약학연구소 1996 약학 논총 Vol.10 No.-

Glibenclamide (GBC) is a well-known antidiabetic agent, which stimulates the secretion of endogenous insulin by pancreatic βcells. However, administration of GBC at a high dose can occasionally induced the fatal hypoglycemia, especially in the patients with impaired liver function. Therefore, in order to develop an oral sustained-release preparation which reduces the side effect, microcapsulation of GBC has been carried out in this experiment. Microcapsules of GBC were prepared by coacervation-phase separation method with ethylcellulose as a wall-forming material in cyclohexane, using polyisobutylene as a coacervation-inducing agent. Different amounts of hydrophilic additives. L-arginine(ARG) and polyethylene glycol 4000 (PEG), were added to the microcapsule wall, in order to alter the porosity of the wall and hence to enhance the release of the core material. The microcapsules prepared were examined for physical properties by scanning electron microscopy (SEM) and particle size analysis. The release of the poorly water-soluble GBC was found to be very slow from the microcapsules and strongly dependent on the core to wall ratio of the microcapsules, but it was accelerated considerably with increasing amount of PEG or ARG. In general, on the basis of dissolution kinetics, GBC releases from microcapsules were followed apparent first-order kinetics, while the dissolution data of GBC powder conformed to Hixon-Crowell's cube root law. Therefore, in conclusion, it might be possible to design the sustained-release formulation by the combination of both GBC powder and microcapsules of different core wall ratio with or without hydrophilic additives.

축력과 모멘트를 받는 가셋트 K형 상관접합부의 강도 추정에 관한 연구

박순정,신의균,김우범,정수영 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

A numerical analysis and finite element analysis were performed to suggest formula of gusset-tube connection that is subjected to axial and lateral load. Through the finite element analysis, the infulence of parameters on the ultimate strength was estimated and design formula was obtained from the case-study of result of the finite element analysis. The formulas suggested by other authors and the analytical of this paper were compared. The primary parameter of the analysis are axial load, lateral load, thickness of the primary menber, diameter of the primary menber and length of the gusset-plate. It was noted that the strength of connetion by the previous proposed formulas was much differ from result of the finite element analysis, but the difference of result between the proposed formula and the finite element analysis was within thirty percentage range. On the future research, small scale and full scale test will be performed to verify a formula suggested by this paper.

황정(黃精) 추출물의 화학구조결정에 관한 연구 (Ⅰ)

윤중호,박주희,김정주,권기락,안철진,주우홍,강진호,신동수 Institute of Genetic Engineering Changwon National 1998 Gene and Protein Vol.2 No.1

본 연구에서는 황정 속에 포함되어 있는 생리활성 물질을 hexane, CHCl₃과 n-butanol층에서 각각의 성분들을 추출하였고, hexane층에서 분리된 화합물 I과 H 중에서, 화합물 I의 봐학구조를 ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC 스펙트럼 및 MS 스펙트럼 등의 분광학적인 방법에 의해 결정하였다. 화합물 I의 구조는 9,12-(9E, 12E)-octadecadienoic acid 임을 확인하였다. In this Paper, biologically active compounds were extracted using organic solvents as hexane. CHC1₃, n-butanol to give each component. Chemical structure of compound I was characterized using ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC spectrum and MS-spectrum, in separated compound I and Ⅱ from hexane layer. Finally, chemical structure of compound I was determined as 9,12-(9E,12E)-octadecadienoic acid.