Anti-Inflammatory and Cytoprotective Effects of TMC-256C1 from Marine-Derived Fungus <i>Aspergillus</i> sp. SF-6354 via up-Regulation of Heme Oxygenase-1 in Murine Hippocampal and Microglial Cell Lines

Kim, Dong-Cheol,Cho, Kwang-Ho,Ko, Wonmin,Yoon, Chi-Su,Sohn, Jae Hak,Yim, Joung Han,Kim, Youn-Chul,Oh, Hyuncheol MDPI AG 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.4

<P>In the course of searching for bioactive secondary metabolites from marine fungi, TMC-256C1 was isolated from an ethyl acetate extract of the marine-derived fungus <I>Aspergillus</I> sp. SF6354. TMC-256C1 displayed anti-neuroinflammatory effect in BV2 microglial cells induced by lipopolysaccharides (LPS) as well as neuroprotective effect against glutamate-stimulated neurotoxicity in mouse hippocampal HT22 cells. TMC-256C1 was shown to develop a cellular resistance to oxidative damage caused by glutamate-induced cytotoxicity and reactive oxygen species (ROS) generation in HT22 cells, and suppress the inflammation process in LPS-stimulated BV2 cells. Furthermore, the neuroprotective and anti-neuroinflammatory activities of TMC-256C1 were associated with upregulated expression of heme oxygenase (HO)-1 and nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in HT22 and BV2 cells. We also found that TMC-256C1 activated p38 mitogen-activated protein kinases (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathways in HT22 and BV2 cells. These results demonstrated that TMC-256C1 activates HO-1 protein expression, probably by increasing nuclear Nrf2 levels via the activation of the p38 MAPK and PI3K/Akt pathways.</P>

Thioacetamide에 의한 BALB/c 마우스 간의 시간별 약물대사효소 억제 양상 : A Time-Course Study

이정운,고우석,김갑호,배연경,하현정,한상섭,천영진,정태천 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

Thioacetamide is a potent hepatotoxicant which requires metabolic activation by cytochrome P450s (P450s) for toxicity. In the present study, the elevation kinetic of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by thioacetamide treatment was investigated in male BALB/c mice. Inaddition, the inhibitory effects of thioacetamide on liver microsomal P450 enzymes were further investigated. Thioacetamide at 100 mg/kg/ was treated intraperitoneally for 6, 12, 24, 36, 48 and 72 hr. The blood was collected at the designated time for assaying the serum enzyme activities. To determine the P450 isozyme-specific activities. ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and benzyloxyresorufin O-debenzylase (BROD) activities were determined for P450 1A1, 1A2 and 2B1, respectively, in liver microsomal fractions. The activities of ALT and AST were started to be elevated 6 hr after thioacetamide treatment andreached the maximun at 36 hr after the treatment. The elevated activities were dramatically recovered at 72 hr. The microscopic exmination of the liver specimen also showed a similar profile of hepatotoxicity. All P450-associated enzyme activities were time-dependently inhibited by the treatiment with thioacetamide. The maximum inhibition of P450 enzymes was observed 36 hr after the treatment. Because the inhibition of P450 enzymes by thioacetamide was time-dependent, our present results suggest that thioacetamide might inhibit P450 enzymes in mechanism-based inactivation.

삼성 타워팰리스 구조시스템 평가

이원호,현창국,강정호,노재천,조중현,송한범 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The purpose of this study was to analyze the housing mobility behavior according to the income change and to predict the probability of upward and downward movements. A binary logit model was used with the data which were obtained by the Korea Research Institute for Human Settlements in 1989. The sample size was 1620 households in Seoul. The results of this study were as follows; the upward movement probability in rental housing or in single housing was higher than that in owned housing or in apartment housing as household income increased.

도시거주자의 혈중 카드뮴 농도와 간 및 신장 기능과의 상관성 평가

김호현,임영욱,임종한,양지연,신동천 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.1

The possibility that liver and kidney function is adversely affected by current levels of environmental exposure to cadmium (Cd) as investigated in adult men and women in the general population in Seoul. From February to August in 2001, blood and morning spot urine samples were collected from 136 not occupationally exposed group (age range 20~75 years) at 4 survey sites throughout Seoul. Liver and kidney function parameters in serum and urine were examined by conventional methods. The questionnaire included factors, i.e. sex, age, smoking, alcohol, diet habit etc. The geometric mean values for Cd in blood (Cd-B) were 1.43 μg/l. It seemed prudent to conclude that liver and kidney function as not disturbed by the current environmental exposure to Cd in study.

강재 슬릿형 댐퍼를 부착한 철근콘크리트조 골조의 거동

신종익,이정한,송한범,강대언,오상훈,차승렬,이용재,김상대,이원호 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

This study investigates the performance of the reinforced concrete frame with the steel plate slit damper. The test was carried out to investigate the structural behavior between the reinforced concrete bare frame and the reinforced concrete frame with the steel plate slit damper. The test results showed that the energy absorption capacity of the damped-frame was several times larger than that of the bare frame. For the damage by the input energy is concentrated on the steel plate slit damper, the ductilities of the former structure were increased and the damaged frame can be reused after exchange the steel plate slit damper.

電氣工學敎育의 發展方向에 關한 硏究

李鎭局,徐相壽,朴魯峰,李忠鎬,劉鍾哲,金洪奎,趙武濟,韓運東,池平植 충주대 2003 産業科學論文集 Vol.11 No.-

In this paper, we discuss actual conditions for running school of electrical and electronic engineering in domestic universities. Also, we catch hold of industrial circles's demands in electrical engineering education and analyze questionary results according to goes into effect school of electrical and electronic & information engineering in major of electrical and electronec engineering's students. Conclusively, we present suitable model in electrical engineering by correction of problems.

자가면역 간염 3증례: 진단 및 치료 반응

박세진, 채희복, 한정호, 윤순만, 박선미, 윤세진 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

자가면역 간염의 정확한 진단을 위해서는 먼저 특징적인 자가면역 간염의 임상 양상과 검사 소견, 그리고 다른 원인에 의한 만성간염의 가능성을 배제할 수 있어야 한다. 본 연구는 2009년에서 2010 년의 자가면역 간염 증례 3례를 살펴 보았다. 아울러 진단 점수표를 이용하여 자가면역성 간염 진단의 객관성을 검토해 보고자 하였다. 본 증례보고는 2009년에서 2010년까지 본 병원에서 자가면역 간염 으로 진단되었던 세 증례에 관한 연구이며, 주로 진단과 치료에 대한 반응을 중심으로 기술하였다. 혈 액을 이용한 간기능 검사는 치료 전과 치료하는 기간 동안 내내 지속적으로 매 1주일마다 시행되었다. 3명의 환자들은 모두 여성으로 평균 나이는 53.7세(48~64)였다. 세 명의 환자 모두에서 표준 치료 요법에 좋은 반응을 나타내었다. 간조직 검사는 2명의 환자에서 시행되었으며 자가면역간염에 부합하 는 병리소견을 나타내었다. 환자들은 prednisolone 단독요법이나 prednisolone과 azathioprine 병 합요법 치료를 받았다. 치료시작 시점으로부터 치료 종료에까지 걸린 시간은 한 달에서 두 달 사이에 분포하였다. 재발은 한 명의 환자에서 치료 종결 후 5개월 째 관찰되었다. 결론적으로 자가면역 간염 환자 세 명은 모두 전형적인 임상양상과 간기능 검사 소견을 나타내었다. 면역 억제 치료제를 투여하였 을 때의 치료반응은 매우 효과적이었으며, 대체로 한 달에서 두 달 사이에 신속하게 나타났다. 재발은 한 명의 환자에서만 관찰되었다.

치과주조용 저카라트 금합금의 시효경화

김형일,김종렬,안호경,장명익,설효정,김교한 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.2

The age-hardening behavior of a commercial dental casting low-carat gold alloy was investigated by means of differential thermal analysis, hardness test, optical microscopic observation and X-ray diffraction study. The following results were obtained. 1. By the isothermal aging of this alloy at 300∼400℃, the hardness increased markedly at the initial stage and continued to increased gradually for the time, and then decreased dractically after longer aging time. 2. The hardening was attributed to the formation of the metastable AuCu I' ordered phase from the α matrix. 3. The overaging with softening was attributed to the precipitation of the fine lamellar structure which was composed of the Ag-rich α1 and AuCu I ordered phases.

조직학적 진단된 chronic necrotizing pulmonary aspergillosis 2예

김연숙,정숙인,기현균,김춘관,김신우,한정호,김성민,백경란,송재훈 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

CNPA는 기존의 폐질환 및 폐절제술로 인해 국소방어기전에 저하가 있거나 비특이적 전신면역상태저하를 갖고 있는 환자들에게서 주로 발생하는 만성적인 공동성 폐질환이다. 현재까지 우리나라에서는 CNPA의 보고가 없는 상태이고, 저자들은 최근 조직학적으로 진단된 CNPA 2예를 경험하였기에 보고하는 바이다. 저자들이 경험한 CNPA 환자들은 만성알코올 중독 및 기관지확장증과 같은 기저질환과 폐절제술을 받은 병력이 있었다. Aspergillus가 폐실질을 침윤하고 있는 조직소견과 조직배양에서 Aspergillus가 검출되어 조직학적 진단이 가능하였고, amphotericin B의 정주요법 및 경구 itraconazole 투여와 폐절제술 등으로 성공적으로 치료되었다. 아직 많은 임상의들에게는 낯선 질환인 CNPA의 치료성적은 환자의 동반질환 및 CNPA 자체의 중증도, 진단과 치료 시작의 지연 등에 의해 크게 좌우되므로 만성적으로 진행하는 공동성 폐병변을 갖고 있는 환자에게서 CNPA를 감별하는 것이 중요하다. Chronic necrotizing pulmonary aspergillosis (CNPA) is a chronic cavitary form of pulmonary aspergillosis. Dozens of CNPA cases have been reported in patients with systemic immunologic dysfunction or altered local defense mechanism from preexisting pulmonary disease. Review of literatures revealed that no CNPA cases have been reported in Korea yet. We experienced two cases of CNPA proven by lung biopsy. A 53-year-old alcoholic male in poor nutritional state was admitted with generalized weakness and weight loss. Chest CT revealed a cavitary nodule surrounded with ground-glass attenuation. CT-guided fine needle aspiration and biopsy was done. The biopsy specimen demonstrated dichotomously branching septated hyphae consistent with those of Aspergillus sp. Another case was a 39-year-old man with bronchiectasis who was admitted with persistent hemoptysis. He had a past history of pulmonary tuberculosis. A parahilar lesion with intracavitary soft tissue mass was incidentally detected in high-resolution GT. Left lingular segmentectomy was done due to uncontrolled hemoptysis and CNPA was histologically diagnosed. Both patients were successfully treated with intravenous amphotericin B followed by oral itraconazole. Even though CNPA is unfamiliar to most clinicians, it should be included in differential diagnoses of chronic progressive cavitary pulmonary lesion, especially in patients with immunologic dysfunction.

Idiopathic colonic varices: an rare cause of Lower gastrointestinal bleeding

( Joung Ho Han ),( Won Joong Jeon ),( Hee Bok Chae ),( Seon Mee Park ),( Sei Jin Youn ) 대한내과학회 2005 대한내과학회 추계학술대회 Vol.69 No.10