운동성 산화 스트레스와 항산화비타민의 보충이 말초단핵세포의 NF-kB 활성에 미치는 영향

진영수,박건구,박준영,김미정,이왕록,김혜영,이한준,박은경 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

Reactive oxygen species(ROS) are implicated in the pathogenesis of a wide variety of human diseases. Numerous studies indicate that ROS may serve as common intracellular molecules that contribute to the process of nuclear factor(NF)κB activation in response to a diverse stimuli. In our laboratory, we have demonstrated tat antioxidants could reverse the decline of immune function caused by exercise-induced ROS. Furthermore, it in necessary to understand a mechanism underlying ROS-dependent disorder in biological system. Recent studies have been shown that several gene expression were regulated by oxidants, antioxidants and other determinants of the intracellular reduction-oxidation(redox) state. In this process, NF-κB have been shown to play a important role. The purpose of the present investigation was to evaluate the effect of exercise-induced oxidative stress and antioxidnt supplementation on NF-κB activation in peripheral mononuclear cells. Forty male SD rats(4 weeks old) were randomly divided into noraml diet group and antioxidants(ATO) supplement group, and then ATO groups were treated with antioxidants(VE: 2001U/kg, VC: 50mg/rat, β-carotene: 300mg/kg, vitamin B6: 250㎍/100g, selenomethionine: 0.1mg/kg) for 16 weeks. After 16 weeks breeding at each condition, each group divided into two groups: Control group(CR) fed generally, Exercise group(CE) fed generally and followed by acute exercise 16 weeks later. Antioxidant Antioxidants and Exercise group(AE) fed with antioxidants and followed by acute exercise. The activation of NF-κB binding activity was increased after exhaustive exercise bout in both group. In addition, pretreatment of ATO group with the antioxidants mixture lead to the inhibition of NF-κB binding activity. This results suggest that NF-κB activation should be further studies in response to a variety of exercise.

당뇨병 환자에서 발생한 대장 가성폐색증 1예

이재형,강준구,박준성,이창범,박용수,김동선,함준수,김태화,안유헌,박충기 대한당뇨병학회 2002 임상당뇨병 Vol.3 No.2

가성 장폐색은 기계적인 폐색부위가 없이 복부팽만과 구토 등 장폐색의 증상과 징후가 나타나는 질환을 말하며, 선행원인을 찾을 수 없는 원발성 가성 장폐색과 다른 질환에 이차적으로 발생하는 속발성 가성 장폐색으로 나눌 수 있다. 당뇨병은 잘 알려진 속발성 가성 장폐색의 원인이지만 실제로 당뇨병에 의한 가상 장폐색의 발생 빈도는 매우 드문 것으로 보이며, 가성 장폐색의 적절한 치료에 대해서는 아직 논란이 있다. 저자들은 2형 당뇨병 환자에게 대장에 국한된 급성 가성 장폐색을 진단하고 보존적 치료를 시행하여 호전된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Colonic pseudo-obstruction is a disorder characterized by gross dilatation of the colon in the absence of any anatomic lesions, which obstructs the flow of the intestinal contents. Diabetes is a well known cause of secondary pseudo-obstruction but its prevalence in rare and not well understood. A 59-year-old woman patient was admitted due to abdominal distension and nausea of a day's duration. She was diagnosed with diabetes 15 years ago, but the condition had been poorly controoled. On the abdominal radiographs, there were marked small and large bowel dilatation with the, exception of the descending colon. Our initial diagnosis was mechanical obstruction, but the possible causes were not found. Also, we could not find another cause of the secondary pseudo-obstruction, with the exception of diabetes mellitus. Thus, she was diagnosed with a diabetes induced intestinal pseudo-obstruction. The colonic dilatation was resolved by conservative management and she became comfortable in 10 hospital days, and was subsequently discharged.

소아의 급성 특발성 혈소판 감소성 자반증에서 ABO 혈액형에 따른 정주용 면역 글로불린의 치료 효과

박경배,민용식,박준수 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Purpose: Idiopathic or immune thrombocytopenic purpura(ITP) is a common childhood illness characterized by thrombocytopenia secondary to shortened platelet survival. Medical therapy includes corticosteroids, intravenous immune globulin (IVIG), and IV Rho(D) immunoglobulin (anti-D). Individuals with Rh-negative blood generally do not respond to treatment with anti-D, but little information is currently available regarding the potential relationship between blood type and response to IVIG. This study was designed to characterize the relationship between ABO and Rh blood type and the response to IVIG in children and adolescents with newly diagnosed ITP. Patients and Method: A retrospective chart review was performed for 31 patients with newly diagnosed ITP initially treated with IVIG by the Department of Pediatrics at Soonchunhyang University Hospital in Seoul and Chunan, Korea. Results: There were no significant differences in response rate or clinical outcome by ABO blood group in children with ITP who received IVIG monotherapy as their initial treatment. However we could not evaluate the effect of Rh type on the reponse to IVIG because our all patients were Rh positive. Conclusion: ABO blood group does not appear to be prognostic factors when IVIG monotherapy is the initial treatment for childhood ITP.

만성 특발성 혈소판 감소성 자반증에서의 Anti-D 정주 치료 4례

박경배,박준수,김영창 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

About 10-20% of patients who present with acute idiopathic thrombocytopenic purpura(ITP) and who have persistent thrombocytopenia for more than 6 months have choronic ITP. Treatment requires differentiating the truly idiopathic state from thrombocytopenias associated with order disorders. Therapeutic options of included corticosteroid, intravenous immunoglobulin(IVIG), and anti-D, as well as splenectomy. Good results were obtained in many chronic ITP patients treated with intravenous anti-D. immunoglobulin. The advantage of anti-D in chronic ITP patients is ease of administration and lower cost as compared to IVIG. We recently treated 4 cases of Rh(D+) chronic ITP patients with intravenous anti-D.

사람의 비후성 반흔에서 면역조직화학기법에 의한 형질전환인자 알파와 표피성장인자 발현의 차이에 관한 면역조직화학적 연구

박영란,김현곤,김종중,문정석,송준섭,설경수,정윤영 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background and Objectives: Transforming growth factor-α (TGF-α) and epidermal growth factor (EGF) are polypeptides which interact with the epidermal growth factor receptor (EGFR) to produce their biological effects. The aim of the present investigation was to elucidate the immunolocalization of TGF-α and EGF in normal human skin, hypertrophic scar with skin graft on soft tissue defect, and postburn hypertrophic scar without graft. Methods: The data presented in this paper focused attention on differences of expression between two kinds of hypertrophic scars in relation to skin graft using immunohistochemistry. Formalin-fixed and paraffin-embedded tissues from 3 normal skin tissues, 3 hypertrophic scars after skin graft and 8 postburn hypertrophic scars without graft were immunolabelled with antibodies directed against TGF-α and EGF. Results: In normal epidermis of skin, strong TGF-α immunoreactivity (IR) was observed in all epidermal layers except the stratum (S.) basale, whereas EGF was immunopositive in a few cells over all epidermal layers. The staining for TGF-α was found in cell membrane, and EGF was seen either diffuse cytoplasmic or peripheral part of cells in normal epidermis. In hypertrophic scar after six months postoperation, TGF-α IR was observed in the upper part of S. spinosum and S. corneum, whereas EGF was diffusely expressed in the S. spinosum, also its intensity was slightly increased compared to normal skin. In hypertrophic scar without skin graft, very strong expression of EGF was detected in the S. spinosum and S. corneum, and intensity of EGF was increased when compared to normal skin and postoperative hypertrophic scar. EGF and TGF-α coexisted in the cells of S. spinosum in normal skin as well as hypertrophic scars. Double-labeled cells were increased in postburn hypertrophic scar compared to normal skin and postoperative hypertrophic scar. In addition, intensity of EGF and TGF-α double IR was the strongest in postburn hypertrophic scar. Conclusion: These results indicate that EGF may have a more complex regulatory role not only in the early stages of wound healing but also in hypertrophic scar.

자외선 조사에 의한 인체 각질형성세포 세포고사 방어인자에 관한 연구

박수홍,박준홍,이종석,황규왕,김계정 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Back ground: Human skin is continuously exposed to UV irradiation. Ultraviolet irradiation of human skin cause sunburn cell which is relevant to the apoptosis of keratinocytes. In the epidermis, apoptosis inducing factors and anti-apoptotic factors probably exist to maintain the integrity of keratinocytes. Objective: The purpose of this study was to investigate the exsistence of apoptosis inducing factors and anti-apoptotic defence factors by evaluation of UV induced apoptosis in cultured human keratinocyte and other keratinocyte cell lines(A-431 cells, KB cells) and its susceptibility of UV induced apoptosis in various conditions. Method: In this study, the percentages of apoptosis, necrosis and cell viability of irradiated human keratinocytes and othe keratinocyte cell lines by MMT assay and AO/EO stain. The percentages of those were measured before UVB irradiation and 8, 24, 48 hours after UVB irradiation. Also, the same evaluations were performed with irradiated human keratinocytes cultured without growth factor and with enough growth factors, both results were compared with each other. And the effect of cycloheximide, a protein synthesis inhibitor was evaluated. Also that of aurintricarboxylic acid(ATA), an inhibitor of endonucleases which play an important role in inducing apoptosis of human keratinocytes was evaluated. Result: Human keratinocytes and other keratinocyte cell lines(A-431 cells, KB cells) were cultured in vitro developed maximal apoptosis 48 hours after irradiation, keratinocytes were more resistant to UV induced apoptosis than the others. The withdrawal of growth factors from keratinocyte and addition of cycloheximide decreased the cell survival rate following UV irradiation and increased the induction of apoptosis. And ATA inhibited UV induced apoptosis. Conclusion: These results indicate that human keratinocytes have both anti-apoptotic factors and apoptosis inducing factors to maintain the homeostasis. And survival signals mediated through growth factors or cellular proteins are responsible for the resistance to apoptosis observed in keratinocytes in vitro.

소아에서 알레르기 증상과 알레르겐 특이항체와의 관련성

박준수,안현철,남해선,황규윤 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

To evulate usefulness of application of MAST-CLA to identify allergens in children, authors conducted MAST-CLA allergen-specific IgE assay and collected allergy related symptoms in 88 pediatric allegic patients diagnosed at Soonchunhyand University Chunan Hospital during October 1998 through January 2000. The mean (SD) age of study subjects was 8.8(3.2) years. The frequent allergic symptoms were rhinorrhea (40.9%), nasal stuffiness(38.6%), cough(36.4%), sneezing(35.2%), and itching sensation(28.4%) in order. Among them, 56.8% revealed at least more than one allergen specific IgE by MAST-CLA. The three most frequent specific allergens were Dermatophagoides farinae(52.3%) Dermatophagies pteronyssinus(51.1%), and house dust(35.2%). The positive rate of allergen specific IgE was increased by increasing age(P<0.001). Among the symptoms the sneezing showed most high positive rate of allergen specific IgE(80.7%) and total IgE(80.7%). In multivariate analysis, significant odds ratios remained(adjusted OR-4.66, P=0.004). In conclusion, our data suggest that allergic symptoms are associated with allergen-specific IgE using MAST-CLA assay, which is an useful measure for identification of causative allergens in pediatric allergen patients.

Indomethacin 子宮內 揷入이 卵巢와 血漿 Hormone에 미치는 影響에 關한 實驗的 硏究

朴俊相,蘿重烈,李壽鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.3

The effects of intrauterine administration of indomethacin on the ovary and plasma steroid hormones were studied. Pseudopregnancy was induced in seven rabbit by the injection of 50 ug of ethinyl estradiol and 100 IU of hCG subcutaneously. And these seven pseudopregnant rabbits served as control group. In the indomethacin-treated group, pseudopregnancy was induced with 50 ug of ethinyl estradiol and 100 IU of hCG, and indomethacin was implanted in uterus on Day seven under laparotomy and uterine incision with pentothal anesthesia. The plasma estrogen and progesterone level was determined by radioimmunoassay with about three days interval in both control and indomethacin-treated group. The uterus and ovaries were submitted to histologic study on Day 30. The following results were obtained; 1. Serum progesterone level in pseudopregnant rabbis was decreased from 15th day of pseudopregnancy and returned to normal values by 18th day, while, in indomethacin-treated group, the high level of serum progesterone was maintained by 7 more days then control group. 2. Serum estrogen levels of pseudopregnant rabbit was higher than pretreatment state and returned to normal value by 30th day, while, in indomethacin-treated group, the serum estrogen values was higher than control group and did not return to normal value by 30th day. 3. The corpus luteum regresses from 15th day in control group, but in indomethacin-treated group, the corpus luteum was maintained till 25th day. The follicles are 4 to 5 in number in control group and 9 to 15 in indomethacin-treated group. The endometrium was papillary growth pattern in control group, but it was functionless in indomethacin-treated group. It seemed that the prostaglandin from the endometrium acts on corporal regression, but it's action on the follicles was different from that on corpus luteum.

체지방 측정기(Futrex-1000 body fat tester)로 측정한 소아의 체지방율에 관한 연구 : 학동기와 청소년기 아동을 대상으로

박상철,이동환,신상만,이상주,박준수,최석민,안재억 순천향대학교 1992 논문집 Vol.15 No.2

Using the infrared interactance(NIR method), the percentage body fat in 1,401 Korean children, aged 6-18years, was evaluated for normal and standard values. Correlation of percentage of body fat with age, weight, height, overweight(%), Kaup index and Rohrer index was also analyzed. The following results were obtained ; 1) In all the subject, weight and height showed standard growth(50-90 percentile) compared to growth data of korean children. 2) The percentage of body fat was decreased with increasing age and was measured higher in female than male. 3) The percentage of body fat was decreased with increasing height in both sex. 4) The percentage of body fat correlated negatively with age, heght, weight in both sex. In boys, the Rohrer index appeared to have a significant positive correlation with the percentage of body fat. (r=0.52454) In girls, the overweight(%) appeared to have a significant positive correlation with percentage of body fat. (r=0.56994) The Kaup index had no correlation with the percentage of body fat. This NIR method is safe, non-invasive, rapid, easy to use, and may be useful predict percentage of body fat, especially in the mass screening.

농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례

박태혁,김정일,손지언,김종국,김형수,정갑열,김준연 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.4

목적 : 농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례 보고. 방법 : 피로감, 전신무력감, 어지럼증, 보행장애 등의 증상을 주소로 내원한 브롬화메틸을 사용하여 방역작업에 종사하는 건강한 20세와 18세의 근로자를 대상으로 혈액검사, 신경정도 및 근전도검사, 뇌자기공명영상 등의 임상검사와 작업력조사를 시행하였다. 결과 : 1례에서 뇌자기공명영상에서 연수와 소뇌의 양측 측충부의 고신호강도가 관찰되었고 11일 후의 추적조사에서 동일 부위의 고신호강토가 감소된 소견이 관찰되었으며 뇌병증으로 최종 진단되었다. 다른 1례는 신경전도검사에서 다발성 신경병 소견과 뇌유발전위검사에서 우측 하부 뇌간부위 병소가 관찰되었고 뇌병증 및 말초신경 병으로 최종 진단되었다. 결론 : 근로자들의 증상은 소독과정에서 브롬화메틸 노출로 인한 신경 병으로 판단되며 훈증소독과방역작업에 종사하는 근로자들의 실태조사와 안전에 대한 대책이 시급하다고 하겠다. Objectives : To report two cases of neuropathy due to methyl bromide intoxication. Methods : Workers, engaged in the fumigating process, complained fatigue, general weakness, ataxia, and hypersomnia. We evaluated them with blood tests, neurophysiologic studies and MRI and investigated their occupational history. Results : Increased signal intensities were found in the medulla oblongata and paraver mian of cerebellum in MRI and after 11 days, high signal intensities were reduced in the following MRI. In the other case, polyneuropathy and rlght lower brainstem lesion were observed In the NCV and BAEP studies. Conclusions : We confirmed that worker's symptoms were related to methyl bromide exposure in the fumigation. It is necessary that we should evaluate present condition of fumigating process and prepare appropriate methods to protect workers engaged in the fumigation.