Effects of Coptidis Rhizoma Herbal Acupuncture Extract on the Acute Gastric Mucosal Lesion Progression Induced by Compound 48/80 in Rats

Mou, Jong-Cheng,Lee, Sena,Kim, Myung-Gyou,Seo, Il-Bok,Leem, Kang-Hyun The Korea Association of Herbology 2013 大韓本草學會誌 Vol.28 No.1

Objectives : Coptidis Rhizoma has been used for stomach disease. However, its property is so cold that it might be avoided to prescribe for the elderly and the infirm having indigestion or diarrhea. Accordingly, the present study was designed to investigate the protective effects of Coptidis Rhizoma herbal acupuncture extract against acute gastric mucosal lesions induced by compound 48/80 in rats. Methods : The Coptidis Rhizoma herbal acupuncture (CRHA) was injected in Choksamni and Chungwan 1 h before compound 48/80 treatment. The animals were sacrificed under anesthesia 3 h after compound 48/80 treatment. The stomachs were removed and the amount of gastric adherent mucus, gastric mucosal hexosamine, SOD, XO, TBARS and histological examination were performed. Results : The decline of gastric adherent mucus, gastric mucosal hexosamine and the histological defects of gastric mucus were significantly protected by CRHA treatment. Gastric adherent mucus in control group was reduced to $38.2{\pm}5.0%$. CRHA groups significantly protected the loss of mucus to $77.5{\pm}4.9%$. Mucosal hexosamine content showed similar patterns. Mucosal hexosamine content in control group was reduced to $45.2{\pm}6.2%$. CRHA groups significantly protected the loss of mucus to $83.0{\pm}7.0%$. The changes of gastric mucosal SOD and TBARS were recovered by CRHA treatment as well. Conclusions : CRHA showed the protective effects on the acute gastric mucosal lesions induced by compound 48/80 in rats. These results suggest that CRHA may have protective effects on the gastritis.

황기(黃기)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향

모충정 ( Jong Cheng Mou ),이세나 ( Se Na Lee ),김명규 ( Myung Gyou Kim ),김명희 ( Myoung Hee Kim ),김형준 ( Hyung Jun Kim ),조학준 ( Hak Jun Jo ),임강현 ( Kang Hyun Leem ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1

Objectives: Elastic fibers are found in the skin, lungs, arteries, veins and other structures. The defects of elastic matrix aggravate hypertension which is associated with alteration in the great arteries, arteries, and arterioles. The elastase inhibitors were undergoing in clinical studies about emphysema and pulmonary hypertension. This study was designed to investigate the effect of Astragali Radix extracts (AR) on elastase activity and anti-oxidative effects. Methods: The elastase inhibitory activity and DPPH (1,1-diphenyl-2-picrylhydrazyl) and NO free radical scavenging activities of AR were measured. Results: The elastase activity was significantly inhibited by AR. The significant DPPH and NO free radical scavenging activities were observed in AR as well. Conclusion: AR showed the anti-elastase effects and anti-oxidative activities in vitro. These results suggest that AR may be a possible drug for the treatment of pulmonary emphysema and pulmonary hypertension.

Anti-inflammatory Effects of Co-treatment of Jingyoganghwaltang and Cheongsimhwan on Croton Oil Induced Hemorrhoid Model in Rats

( Nayoung Jo ),( Jong-cheng Mou ),( Kanghyun Leem ),( Taeyeon Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.6

Objectives : Hemorrhoids are one of the most common diseases in humans. Jingyoganghwaltang (JG) and Cheongsimhwan (CS) have been used for treating hemorrhoids in Korean traditional clinical practice. The present study was designed to evaluate the traditional effects of JG and CS on the experimental hemorrhoid model in rats. Methods : Hemorrhoids are closely related to inflammation. Accordingly, we examined the nitric oxide (NO) production in macrophage cell line in order to evaluate the anti-inflammatory effect. The expression levels of inflammation related genes including IL-1 beta, IL-6, INOS, and TNF-alpha were examined via a real-time quantitative PCR. Croton oil-induced hemorrhagic animal model was used to test the in vivo efficacy against hemorrhoids. The rectal tissues were weighed and the inflammatory proteins were measured to confirm the anti-inflammatory effects. Results : JG and CS have a statistically significant effect on inhibition of NO production and on the reduction of inflammatory gene expression such as IL-1 beta, IL-6, INOS, and TNF-alpha. The synergistic effects of co-treatment of JG and CS were found out in the IL-6 gene expression. The in vivo study using croton oil-induced hemorrhoid model in rat was performed to check the co-treatment effects. As a result, the co-treatment reduced the inflammation of the rectal tissue and decrease the inflammation related protein productions including ICAM1, MMP2 and MMP9. Conclusions : These results suggest that JG and CS co-treatment demonstrated anti-inflammatory effects in croton oil-induced hemorrhoid model in rat.

Anti-inflammatory Effects of Co-treatment of Jingyoganghwaltang and Cheongsimhwan on Croton Oil Induced Hemorrhoid Model in Rats

Jo, Nayoung,Mou, Jong-Cheng,Leem, Kanghyun,Kim, Taeyeon The Korea Association of Herbology 2018 大韓本草學會誌 Vol.33 No.6

Objectives : Hemorrhoids are one of the most common diseases in humans. Jingyoganghwaltang (JG) and Cheongsimhwan (CS) have been used for treating hemorrhoids in Korean traditional clinical practice. The present study was designed to evaluate the traditional effects of JG and CS on the experimental hemorrhoid model in rats. Methods : Hemorrhoids are closely related to inflammation. Accordingly, we examined the nitric oxide (NO) production in macrophage cell line in order to evaluate the anti-inflammatory effect. The expression levels of inflammation related genes including IL-1 beta, IL-6, INOS, and TNF-alpha were examined via a real-time quantitative PCR. Croton oil-induced hemorrhagic animal model was used to test the in vivo efficacy against hemorrhoids. The rectal tissues were weighed and the inflammatory proteins were measured to confirm the anti-inflammatory effects. Results : JG and CS have a statistically significant effect on inhibition of NO production and on the reduction of inflammatory gene expression such as IL-1 beta, IL-6, INOS, and TNF-alpha. The synergistic effects of co-treatment of JG and CS were found out in the IL-6 gene expression. The in vivo study using croton oil-induced hemorrhoid model in rat was performed to check the co-treatment effects. As a result, the co-treatment reduced the inflammation of the rectal tissue and decrease the inflammation related protein productions including ICAM1, MMP2 and MMP9. Conclusions : These results suggest that JG and CS co-treatment demonstrated anti-inflammatory effects in croton oil-induced hemorrhoid model in rat.

Effects of Coptidis Rhizoma Herbal Acupuncture Extract on the Acute Gastric Mucosal Lesion Progression Induced by Compound 48/80 in Rats

( Sena Lee ),( Jong Cheng Mou ),( Myung Gyou Kim ),( Il Bok Seo ),( Kang Hyun Leem ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.1

Objectives : Coptidis Rhizoma has been used for stomach disease. However, its property is so cold that it might be avoided to prescribe for the elderly and the infirm having indigestion or diarrhea. Accordingly, the present study was designed to investigate the protective effects of Coptidis Rhizoma herbal acupuncture extract against acute gastric mucosal lesions induced by compound 48/80 in rats, Methods : The Coptidis Rhizoma herbal acupuncture (CRHA) was injected in Choksamni and Chungwan 1 h before compound 48/80 treatment. The animals were sacrificed under anesthesia 3 h after compound 48/80 treatment. The stomachs were removed and the amount of gastric adherent mucus, gastric mucosal hexosamine, SOD, XO, TBARS and histological examination were performed, Results : The decline of gastric adherent mucus, gastric mucosal hexosamine and the histological defects of gastric mucus were significantly protected by CRHA treatment. Gastric adherent mucus in control group was reduced to 38.2 ± 5.0%. CRHA groups significantly protected the loss of mucus to 77.5 ± 4.9%. Mucosal hexosamine content showed similar patterns. Mucosal hexosamine content in control group was reduced to 45.2 ± 6.2%. CRHA groups significantly protected the loss of mucus to 83.0 ± 7.0%. The changes of gastric mucosal SOD and TBARS were recovered by CRHA treatment as well, Conclusions : CRHA showed the protective effects on the acute gastric mucosal lesions induced by compound 48/80 in rats. These results suggest that CRHA may have protective effects on the gastritis.