Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

한국산 배의 Polyphenol 화합물군이 쥐의 면역기능에 미치는 영향

최희진,한호석,박정혜,배종호,우희섭,안봉전,배만종,김현구,최청 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.4

This study was conducted to investigate immunofunctional activity of the polyphenol fractions isolated from Korean pear. In the experimental of Rosette forming cell, the results showed that all the polyphenol fractions enhance the cell count compared with the control group. Especially polyphenol fraction II and III showed highly significant effect on Rosette forming cell, and allergy inhibition. After antigen challenge, histamine content of blood of the polyphenol groups was lowered to near the normal group.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

단기간의 금연과 일회성 유산소 운동이 흡연자의 심혈관 반응에 미치는 영향

송준경(Jun Kyung·choi),최현민(Hyun Min Choi),김종경(Jong Kyung Kim),노호성(Ho Sung Nho) 한국사회체육학회 2012 한국사회체육학회지 Vol.0 No.48

The purpose of this study was to investigate cardiovascular responses to a bout of aerobic exercise and acute smoking cessation in smoker. nine collegiate male smoker who have been smoking(duration of smoking: 7.5 ± 0.9yrs; number of cigarettes per day: 15.5 ± 1.5) participated in this study. All subjects performed PEI to stimulate Group IV metaboreceptor at immediately after smoking, 24hours after smoking cessation and smoking after a bout of aerobic exercise. they were measured SBP, DBP, HR, SV, CO, TVC using by finometer(FMS co, Netherland). As a result, compared to immediately after smoking, SBP, DBP, and MAP were significantly decreased 24-hours after smoking cessation at rest and PEI(p<0.05). In addition, smoking after a bout of aerobic exercise caused by post-exercise hypotension indicated that SBP was significantly decreased compared to immediately after smoking(p<0.05). also, the changes in DBP and MAP at rest and handgrip exercise were significantly smaller compared to immediately after smoking(p<0.05). Thus, the results showed that smoking cessation during 24-hours and a bout of aerobic exercise relieve excess blood pressure response and enhanced arterial baroreflex in collegiate male smoker. It is concluded that caused exercise pressor reflex by stimulated Group IV may be effective to improve excess blood pressure responses.

백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

최근 10년간 원광의대병원에서 시행한 소아 심장혈관 수술의 임상적 고찰

윤향석,정수미,최두영,오광수,오연균,김종덕,양현웅,이삼윤,김형곤,최종범,최순호,노병석 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

We reviewed 10 years experiences of the clinical aspects and early postoperative results in 544 pediatric patients(age less than 16 years old). Among them. 529 cases had the congenital heart diseases, and 15 had the acquired diseases. Open heart surgery was performed in 413 patients. 115 cases were treated with non-open heart surgery. 16 with interventional catheterization. Annual increase of the cardiac patients was not significant in recent 10 years, except slight increase in 1994. The mean age of the study patients had been evidently changed to younger year by year. Total mortality rate of the open heart surgery was 6.4%. The cyanotic congenital heart diseases were high in early postoperative mortality by 25.7%, whereas 4% in acyanotic group. The age group less than one month was most highest in surgical mortality(66.7%). There were no deaths in patients with patent ductus arteriosus(103 cases), atrial septal defect(83 cases) or pulmonary stenosis(17 cases). Among 220 patients with ventricular septal defects(VSD). 10(4.5%) were died early postoperatively. In cyanotic group, the patients with pulmonary atresia with ventricular septal defects(PAVSD), transposition of the great arteries(TGA), interrupted aortic arch(IAA), complex cardiac anomalies with isomerism seemed to be most susceptible to an early death.

短距離競走의 記錄向上을 위한 技術指導 硏究

崔鍾浩 대구교육대학교 1985 論文集 Vol.21 No.-

A short distance race requires a variety of individual, technical qualities as well as physical, scientific guiding principles on which it could be based. The most important qualities for the record improvement in a short distance race most speed and its continuance. In addition, muscular power, especially that of bodily movement is necessary in order to increase the explosive speed. For the acceleration of speed, the cooperative functioning of the body is required, such as the fast change of nervous system, the clearance of the foreground, continuing the acceleration while lengthening stride, and the driving force of arms and legs for keeping acceleration. It is necessary for the sprinter to avoid the unnecessary motion of the whole body at the moment of speed rising in order in order that the increased speed could be maintained. In particular, acquisition of excellent rhythm, balance, and timing of the body as well as controling of cooperative nerve and muscle are effective for the record improvement through the consummate skill. The record improvement in a short distance race would be possible through the practice of scientific technique and the development of reasonable guiding methods, all of which would be highly contributed to the development of the whole events of athletic sports.

프로축구팀에 대한 지역주민들의 사회문화적 인식

최동순,이창섭,이종호 충남대학교 체육과학연구소 2006 體育科學硏究誌 Vol.24 No.1

This study is on sociocultural recognition of regional residents for professional football dub, Daejeon Citizen FC. For this 348 persons were participated in survey. Two Analyses were performed : 1) One-way analysis of varience that investigates the significant differences of sociocultural recognition among sex, age, school career, income, and social class, participation degree for soccer activities and interest degree for soccer; 2) multiple regression analysis that investigates the sociocultural cognitions(regional identity, social interaction, cultural life) of regional residents for Daejeon Citizen FC(existence, game, relevance with residents). The result of above analyses gained following conclusions : 1) There are significant differences on sociocultural recognition among sex, social class, participation degree for soccer activities and interest degree for soccer. 2) Game, relevance between residents and Daejeon Citizen FC contribute to reinforcement of regional identity, social interaction and cultural life of residents; existence of Daejeon Citizen FC do not contribute to them instead.

골프 드라이브 스윙시 구질 변화에 따른 운동학적 분석

최성진,박종진,양동호 한국운동역학회 2002 한국운동역학회지 Vol.12 No.2

본 연구에서는 볼 구질의 변화에 따른 스윙 동작의 운동학적인 변인을 3차원적으로 비교 분석하여 스윙 동작의 운동역학적인 원리를 제시하며, 볼 구질이 다른 스윙 동작의 차이점을 밝히는 데 목적이 있으며, 프로골퍼로 경력 3년 이상의 남자 선수 7멍을 대상으로 스트레이트 성공, 페이드 성공과 실패로 구분하여 골프 스윙의 운동학적 변인들을 과학적으로 제시하기 위해 비교 분석한 결과 다음과 같은 결론을 얻었다. 페이드 성공시에는 임팩트를 지나서도 계속 손목의 각도가 근소한 차이로 줄어드는 것으로 나타났고 실패시에는 각 프레임의 각도가 규칙적으로 변하지 않게 나타났다. 이는 스트레이트 성공과 페이드 성공에 대한 손목각도의 차이가 영향을 미치는 것으로 나타나 다른 요인과 손목각도와의 관계를 분석하는 것이 필요하다고 하겠다. 페이드 실패의 경우는 임팩트시의 몸통 비틀림각도가 성공때 보다 작아지면서 클럽헤드가 오픈되지 못한 것으로 나타났다. 골프스윙의 형태의 변화로 인하여 어깨관절의 회전력을 더 많이 사용하여 파워을 증진시키기 위한 동작이며, 골프 스윙에서 어깨회전 운동은 축을 중심으로 회전하기 때문에 어깨회전각도의 차이는 없는 것으로 나타났다. 스트레이트 성공시 보다 페이드 성공시에 고관절의 회전각도가 점진적으로 일정하게 각이 커졌다. 이는 고관절의 회전을 더 크게 하여 지연히팅을 보다 자연스럽게 한 것으로 나타났다. 페이드 볼 구사시 임팩트 존에서 클럽의 페이스가 닫히는 것과 팔의 자연스러운 롤링 동작을 억제시키는 것으로 나타났다. 본 연구에서는 구질 변화에 따른 골프 스윙 동작을 스트레이트 성공, 페이드 성공과 실패시로 구분하여 운동학적 변인들을 3차원 동작 분석을 통하여 경기력 향사에 도움이 되고자 하였으며 앞으로 많은 연구자들이 각 종목에 있어서 경기력 향상을 위한 역학적인 분석이 계속 진행되어져야 할 것이다. This study divided straight success, pade success and failure with 7male golfers who have experiences more than 3 years, analyzed kinematic factors of golf swing to suggest scientifically. The conclusions were follows. 1) The wrist angle has significant difference in straight success ,pade success and failure when swing of every pattern. There is no significant difference in pade success and failure. 2) The body twist angle has no significant difference in straight success ,pade success and failure when swing of every pattern. There is no significant difference in pade success and failure. 3) The shoulder joint rotation angle has no significant difference in success ,pade success and failure when swing of every pattern. There is no significant difference in pade success and failure. 4) The left hip joint vertical angle has no significant difference in straight success ,pade success and failure when swing of every pattern. There is no significant difference in pade success and failure. 5) The hip joint rotation angle has no significant difference in straight success ,pade success and failure when swing of every pattern. There is no significant difference in pade success and failure. 6) The trunk angle has no significant difference in straight success ,pade success and failure when swing of every pattern. There is no significant difference in pade success and failure. 7 )The left knee joint angle has no significant difference in straight success ,pade success and failure when swing of every pattern. There is no significant difference in pade success and failure. This study divided golf swing motion of pattern change in straight success, pade success and failure and analyzed the kinematic factors by 3-dimension cinematography to improve performance In the future, many researchers have to study kinematic analysis to improve performance in every events.