소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

낭성법랑모세포종, 함치성낭, 치성각화낭의 방사선소견과 Ki-67, PCNA, Cytokeratin 발현과의 연관성에 관한 연구

송만용,이삼선,이진구,이원진,허민석,이재일,민병무,최순철 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To compare the proliferation potential of the epithelial cells between unicystic ameloblastoma (UA), dentigerous cyst (DC), and odontogenic keratocyst (OKC) and to correlate this proliferation potential with the radiographic features of these three pathoses. Materials and Methods : Immunohistochemical expression of PCNA, Ki-67, and cytokeratin as a proliferation marker were assessed for 15 cases of UA, 15 cases of DC, and 15 cases of OKC. The degree of immunochemical expression of three proliferation markers were correlated with the radiographic features, especially cortical expansion (negative and positive) and shape of border (scalloped and round). Results : Using PCNA and Ki-67, OKC showed the highest proliferation potential and UA the lowest. Statistically significant differences were found between the OKC and the UA (p.0.05). However, no statistically significant difference was present according to the radiographic features in all pathoses. Using cytokeratin, there was no significant differences of proliferation potential among three pathoses. Conclusions : OKC epithelium has the most intense proliferation potential, followed by the dentigeous cyst and then unicystic ameloblastoma. There is no significant relation between the radiographic features and the proliferation potential of epithelium of these three pathoses.

1년간의 자발적인 운동참여가 중년 남성 제2형 당뇨병 환자의 혈중 지질 성분에 미치는 영향

허만동(Man Dong Huh),이진훈(Jin Hoon Lee),류승필(Sung Pil Ryu) 한국발육발달학회 2006 한국발육발달학회지 Vol.14 No.3

The purpose of this study is to investigate the effects of voluntary exercise participation on blood lipids in type II diabetes mellitus middle-aged men. Eighteen male volunteers were recruited and exercised, jogging, cycle ergometer, swimming, in the sports center for 12 months without any supervision. They had educated heart rate calculation method to keep their target heart rate during exercise. Before and after the training, their body composition, maximal oxygen uptake, and blood lipids profiles were analyzed. Body weight was not changed during experimental periods; however body fat ratio was statistically decreased at the same periods. Blood glucose, TC, LDL-C, and TG were decreased significantly and HDL-C was significantly increased (p<.05). RQ was significantly lower compared to the before exercise and their maximal oxygen consumption and exercise time to fatigue were increased significantly (p<.05). These results suggested that voluntary exercise with simple exercise prescription was also effectual to enhance blood lipids in type II diabetes mellitus middle-aged men.

건진센터 수검자 자료를 이용한 비만지표에 대한 연구

김상만,최희정,이득주,김광민,윤수진 대한비만학회 1997 The Korean journal of obesity Vol.6 No.2

We have examined l5145 persons(7468 men 7677 women) to determine the role of Obesity Index(body mass index, WHR, body fat percent) on the risk of various chronic disease. All the subjects were pooled from Health Examination Center who took the annual health examination from June, 1994 through Jan, 1997, BMI(Body mass index, kg/㎡), WHR(Waist hip ratio) were measured and correlated with the frequency of chronic disease. In order to evaluated the BMI, WHR, Body Fat as an independent factors on rnorbidity, analysis of covariance was applied adjusted for age. The risk of variows chronic diseases were also analyzed with obesity, defined by BMI above 26kg/㎡ (men), 25kg/㎡ (women), by logistic regression adjusted age and sex. Risk of hypertension was 2.6 with 95% eonfidence interval of 1.9-2.8 in obese group. Risk of diabetes mel1itus, hypercholesterolemia, hypertriglyceridemia, gall stone, fatty liver were 3.2(2.3-4.4), 1.2(0.9-1.4), 2.1(1.7-2.8), 1.4(1.1-2.6) and 1.3(0.8-2.3) respectiveiy. The risk of various chronic diseases were also analyzed with obesity, WHR above 0.9(men), 0.81(women), by logistic regression adjusted age and sex. Risk of hypertension was 1.7 with 95% confidence interval of 1.4 -2.1 in obese group. Risk of diabetes mellitus, hypercholesterolemia, hypertriglyceridemia, gall stone, fatty liver were 3.1(2.3-4.0), 1.2(0.9-l.4), 1.4(1.1-2.6), 2.4(l.8-4.8) and 2.1(1.2-4.1) respectively. The BMI, WHR were independent risk factors for various chronic diseases.

경막외마취 환자에서 10% Pentastarch 용액의 혈장확장효과

이정은,박병룡,김은경,김만수,최세진 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

In order to compare the effect of 10 % pentastarch solution with Ringer's lactate solution on plasma volume expansion in thirty epidural anesthetic patients, systolic arterial blood pressure (SABP), diastolic arterial blood pressure(DABP), heart rate(HR), central venous pressure(CVP), cardiac output(CO) and changes of hemoglobin(Hb) were measured. The patients were devided into three groups Group Ⅰ (n=10) ; Epidural anesthesia was performed without fluid infusion previously(control) Group Ⅱ (n=10) ; 500 ml of Ringer's lactate solution was rapid infused intravenously before epidural anesthesia Group Ⅲ (n=10) ; 500 ml of 10% pentastarch solution was rapid infused intravenously before epidural anesthesia All data were collected before fluid infusion, at 15 min, at 30 min, at 45 min, at 60 min, at 120 min and at 180 min after epidural local anesthetic injection. The results are as follows. 1. SABP, DABP, HR, CVP and CO were decreased significantly (p<0.05) at 30, 45 minute after local epidural anesthetic injection in Group Ⅰ and Group Ⅱ. There was no any significant differences between two groups. 2. SABP, DABP, HR and CO were not changed significantly, Besides CVP was increased significantly (p<0.05) after 10% pentastarch solution infusion in group Ⅲ. 3. Changes of Hb were increased in group Ⅲ.

씨고자리파리의 에스테라제에 대한 Dichlorvos의 저해효과

이종진,최만영 전북대학교 유전공학연구소 1989 遺傳工學硏究所報 Vol.2 No.1

씨고자리파리의 변태에 따른 단백질 및 에스테라제의 변화, 에스테라제의 활성에 대한 dichlorvos의 영향, 그리고 에스테라제의 α-Naphthylacetate 가수분해 활성에 대한 dichlorvos의 저해효과를 전기영동법과 Gomori의 효소활성 측정법에 의해 조사한 결과 총 28개의 단백질과 14개의 에스테라제 밴드를 분리하였으며, dichlorvos의 중앙치사약량은 암컷성충에서 15.76μg/g, 3령 중기 유충에서 52.17㎍/g이었다. Dichlorvos 처리후 암컷 성충의 에스테라제 밴드의 변화가 관찰되었다. 雌成蟲 homogenate의 전체 esterase 활성중 약 40.9%가 dichlorvos농도 약 6×10^-5M에 의해서 沮害를 받은 것으로 나타났다. Changes in protein and nonspecific soluble esterase patterns, the effect of dichlorvos on esterase activity, and the inhibition of α-Naphthylacetate hydrolyzing activity of esterase by various concentrations of the dichlorvos in the seedcorn maggot, Delia platura(Meigen), were investigated using PAGE and Gomori's colorimetric methods. Through all stages, 28 electrophoretic protein bands and 14 esterase isozyme bands were separated. LD 50 values of the dichlorvos on the larvae and female adults were 52.17μg/g and 15.76μg/g, respectively. Difference of electrophoretic esterase isozyme band patterns between knock-downed, survived and control adults after treatment of dichlorvos were observed. 40.9% of esterase activity to a-Naphthylacetate in homogenate of female adults was inhibitable by 6×10^-5M dichlorvos.

Rifamycin 발효에 관한 연구 - 2단 전배양 및 배양 유도기 단축에 관한 연구 -

이진만 東國專門大學 1997 金龜論叢 Vol.4 No.1

표준 rifamycin 2단 전배양에 있어서 배지조성의 강화를 위하여 soy bean flour, corn steep liquor 및 barbiturate등을 보강 또는 첨가하여 전배양 배지를 고농도화함으로서 균체증식 촉진과 본 배양 발효시 유도기 단축으로 rifamycin 생성능을 향상시켰다.

천마(天麻, Gastrodia elata Blume) 종균배양의 최적화에 대한 연구

이진만,김성호 東國專門大學 2002 金龜論叢 Vol.9 No.1

천마는 뽕나무버섯과 공생관계로 버섯에 의한 영양공급에 의해서만 생육이 가능한 것으로 알려져 있다. 따라서 천마의 다수확재배를 위한 가장 중요한 인자중의 하나로 우수한 품질의 종균배양은 천마의 재배지의 활착율에 큰 영향을 미치므로 생육 및 수확량, 재배기간 등과 직결된다고 생각된다. 한편 5-6년 전부터 경북 칠곡군 관내에서 대량인공 재배되기 시작하여 그 재배방법에 대한 기초연구가 절실한 실정이다. 따라서 천마재배에 대한 기초연구로서 천마의 종균배양의 최적화를 통해 천마의 다수확재배법의 구축을 위한 기초연구를 수행한 결과, 종균배양에 있어서 액체배양시 물에 용해될 때 친수기 부분이 이온으로 해리되지 않는 계면활성제인 Tween80의 첨가량을 0.0066%, 5mm glass bead ball 30개를 첨가하여 배양시 pulpy growth를 유도할 수 있었으며, 균사체의 질과 양적인 면에서 가장 우수하였다. 따라서 상기의 조건에서 배양한 종균을 참나무 톱밥배지에 5%접종하여 45일간 종균 배양후 25℃, 60%의 습도조건에서 참나무 활착도가 가장 우수하였다.

N- 및 -4-Amino-N-(6-Alkyl-, 6-Alkoxy-2-Benzothiazolyl) Benzenesulfonamides類의 合成과 抗菌作用에 關한 硏究

李萬佶,閔庚鎭 嶺南大學校 環境問題硏究所 1984 環境硏究 Vol.4 No.1

Eight new sulfonamides containing benzothiazole ring at N¹ were prepared. The compounds were synthesized by reacting 2-amino-6-methyl-, -6-ethyl-, -6-methoxy-, -6-ethoxybenzothiazole with benzenesulfonyl chloride or p-acetamidobenzenesulfonyl chloride, respectively. All the other analytical methods, as well as IR, NMR, elemental analysis, agreed with the proposed structures. The compounds exhibited antibacterial activity, in vitro, against Gram positive, Staphylococcus aureusa, and Gram negative, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae at concentrations of 100 to 250㎍/㎖. And they also exhibited antifungal activity against Cryptococcus neoformans at concentrations of 50 to 250㎍/㎖. It is of interest that these compounds revealed significant antifungal activity. This result means that these compounds are different from other sulfonamides already developed.

HP/3000에서 연속적인 값을 갖는 시스템의 모의 실험 방법에 관한 연구

李萬鎬,劉寬鍾,陳成日 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.2

There are four methods of solving differential equations appearing in engineering problems. First, analytic method. Second, numerical analytic method using digital computer. Third, method using analog computer. Last, method which simulates analog computer by using digital computer processors. In case where me equations are non-linear, the first and the second method are very difficult and the third method is not used well because of the rareness of the analog computer. So it is economical to simulate the analog computer by using digital computer widely used. But, since mere are no such digital-analog simulation processors in HP/3000 computers currently in KOREA, many computer users have difficulties in solving problems. In this paper, we design and implement a simulation processor which enables the fourth method in HP/3000 computers.